In the present study, a green, sustainable, simple and low-cost method was adopted for the synthesis of ZnO NPs, for the first time, using the aqueous extract of sea lavender, Limonium pruinosum (L.) Chaz., as a reducing, capping, and stabilizing agent. The obtained ZnO NPs were characterized using ultraviolet–visible spectroscopy (UV–VIS), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), energy-dispersive X-ray analysis (EDX), transmission electron microscopy (TEM), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). The UV–Vis spectra of the green synthesized ZnO NPs showed a strong absorption peak at about 370 nm. Both electron microscopy and XRD confirmed the hexagonal/cubic crystalline structure of ZnO NPs with an average size ~ 41 nm. It is worth noting that the cytotoxic effect of the ZnO NPs on the investigated cancer cells is dose-dependent. The IC₅₀ of skin cancer was obtained at 409.7 µg/ml ZnO NPs. Also, the phyto-synthesized nanoparticles exhibited potent antibacterial and antifungal activity particularly against Gram negative bacteria Escherichia coli (ATCC 8739) and the pathogenic fungus Candida albicans (ATCC 10221). Furthermore, they showed considerable antioxidant potential. Thus, making them a promising biocompatible candidate for pharmacological and therapeutic applications.

Although, different plant species were utilized for the fabrication of polymorphic, hexagonal, spherical, and nanoflower ZnO NPs with various diameters, few studies succeeded in synthesizing small diameter ZnO nanorods from plant extract at ambient temperature. This work sought to pioneer the ZnO NPs fabrication from the aqueous extract of a Mediterranean salt marsh plant species Limoniastrum monopetalum (L.) Boiss. and assess the role of temperature in the fabrication process. Various techniques have been used to evaluate the quality and physicochemical characteristics of ZnO NPs. Ultraviolet–visible spectroscopy (UV–VIS) was used as the primary test for formation confirmation. TEM analysis confirmed the formation of two different shapes of ZnO NPs, nano-rods and near hexagonal NPs at varying reaction temperatures. The nano-rods were about 25.3 and 297.9 nm in diameter and in length, respectively while hexagonal NPs were about 29.3 nm. The UV–VIS absorption spectra of the two forms of ZnO NPs produced were 370 and 365 nm for nano-rods and hexagonal NPs, respectively. FT-IR analysis showed Zn–O stretching at 642 cm⁻¹ and XRD confirmed the crystalline structure of the produced ZnO NPs. Thermogravimetric analysis; TGA was also used to confirm the thermal stability of ZnO NPs. The anti-tumor activities of the two prepared ZnO NPs forms were investigated by the MTT assay, which revealed an effective dose-dependent cytotoxic effect on A-431 cell lines. Both forms displayed considerable antioxidant potential, particularly the rod-shaped ZnO NPs, with an IC₅₀ of 148.43 µg mL⁻¹. The rod-shaped ZnO NPs were superior candidates for destroying skin cancer, with IC₅₀ of 93.88 ± 1 µg mL⁻¹ ZnO NPs. Thus, rod-shaped ZnO NPs are promising, highly biocompatible candidate for biological and biomedical applications. Furthermore, both shapes of phyto-synthesized NPs demonstrated effective antimicrobial activity against various pathogens. The outcomes highlight the potential of phyto-synthesized ZnO NPs as an eco-friendly alternative for water and wastewater disinfection.

Lake Edku receives considerable amounts of domestic, agricultural, and industrial waste water. Accordingly, the response of benthic foraminifera to heavy metal pollution has been assessed. Surficial sediment samples were collected at 11 stations throughout the lake during June and December 2013. At each station, pH, dissolved oxygen, temperature, salinity, transparency, water depth, concentrations of Pb, Cu and Cd were measured. Benthic foraminiferal distribution and its relationship with environmental parameters and heavy metal concentrations have been investigated using redundancy analysis (RDA) and cluster analysis. The resulted data show that the foraminiferal distribution was significantly affected by these heavy metals pollution. The distribution pattern shows great dominance of Ammonia tepida which confirms its tolerance to heavy metals pollution especially Cu and Cd. Porosononion spp.correlates positively with Cu, while Saccorhiza ramosa correlates positively with Pb and Cd. The most sensitive species to higher concentrations of heavy metals is Ammonia Parkinsoniana.It has negative correlation with Pb, Cu and Cd. Limited number of living foraminiferal specimens, low foraminiferal density and diversity have been recorded in Lake Edku. Relatively high percentage of deformed foraminiferal tests (~ 21%) has been observed in the lake.