Diffusion and immobilization of molecules in biomembranes are essential for life. Understanding it is crucial for biomimetic approaches where well-defined substrates are created for live cell assays or biomaterial development. Here, we present biomimetic model systems consisting of a supported lipid bilayer and membrane coupled proteins to study the influence of lipid–lipid and lipid–protein interactions on membrane mobility. To characterize the diffusion of lipids or proteins, the continuous photobleaching technique is used. Either Neutravidin coupled to DOPE-cap-Biotin lipids or GFP coupled to DOGS-NTA lipids is studied at 0.005–0.5 mol% concentration of the linker lipid. Neutravidin creates mobile obstacles in the membrane, while GFP coupling results in immobile obstacles. By actin filament coupling to Neutravidin-lipid complexes, obstacles are crosslinked, resulting in lipid mobility reduction along with the appearance of a membrane texture. Theoretical considerations accurately describe lipid diffusion changes at high obstacle concentration as a function of obstacle size and viscous effects. The mobility of membrane lipids depends on the concentration of protein-binding lipids and on the concentration and charge of the coupled protein. Next to diffusion and friction coefficients, we determine the effective obstacle size as well as a charge-dependent effect that dominates the decrease in lipid mobility.

The human iron storage protein ferritin represents an appealing template to realise a semisynthetic magnetic nanoparticle (MNP) for spatial manipulation or inductive heating applications on a nanoscale. Ferritin consists of a protein cage of well-defined size (12 nm), which is genetically modifiable, biocompatible, and into which a magnetic core is synthesised. Here, we probe the magnetic response and hence the MNP’s suitability for (bio-)nanotechnological or nanomedical applications, when the core is doped with 7 % cobalt or 7 % zinc, in comparison to the undoped iron oxide MNP. The samples exhibit almost identical core and hydrodynamic sizes, along with their tunable magnetic core characteristics as verified by structural and magnetic characterisation. Cobalt doping significantly increased the MNP’s anisotropy and hence the heating power in comparison to the other magnetic cores with potential application as a mild heat mediator. Spatial magnetic manipulation was tested with MNPs inside droplets, the cell cytoplasm, or the cell nucleus, where the MNP surface conjugation with mEGFP and poly(ethylene glycol) gave rise to excellent intracellular stability and traceability within the complex biological environment. A magnetic stimulus (smaller than fN forces) results in the quick and reversible redistribution of the MNPs. The obtained data suggest that semisynthetic ferritin MNPs are highly versatile nanoagents and promising candidates for theranostic or (bio-)nanotechnological applications.