Understanding how endangered carnivores partition spatiotemporal distribution in human-dominated landscapes is pivotal for mitigating biodiversity loss in climate-sensitive boreal ecosystems. Here, we used kernel density data derived from a 16-month camera-trap survey (140 UVL7 cameras), cold single-season (November–April) occupancy models, and MaxEnt 3.4.4 to identify the effects of biotic interactions, anthropogenic disturbance, and environmental factors on the spatiotemporal distribution of the wolverine (Gulo gulo) in Beijicun National Nature Reserve, Heilongjiang Province, China. We found that wolverines exhibited crepuscular activity patterns using night-time relative abundance index (NRAI) = 50.29% with bimodal peaks (05:00–07:00, 13:00–15:00), with dawn activity predominant during the warm season (05:00–06:00) and a bimodal activity pattern in the cold season (08:00–09:00, 14:00–15:00). Temporal overlap with prey (overlap coefficient Δ = 0.84) and competitors (Δ = 0.70) was high, but overlap with human-dominated temporal patterns was low (Δ = 0.58). Wolverines avoided human settlements and major roads, preferred moving along forest trails and gentle slopes, and avoided high-altitude deciduous forests. Populations were mainly concentrated in southern Hedong and Qianshao Forest Farms, which are characterized by high habitat integrity, high prey densities, and minimal anthropogenic disturbance. These findings suggest that wolverines may influence boreal trophic networks, especially in areas with intact prey communities, competitors, and spatial refugia from human disturbances. We recommend that habitat protection and management within the natural reserve be prioritized and that sustainable management practices for prey species be implemented to ensure the long-term survival of wolverines.

The global focus on fostering harmonious interactions and promoting rational coexistence among wildlife species to uphold or reinstate biodiversity remains a prominent area of interest. We conducted a study on the sable and yellow-throated marten in Taipinggou National Nature Reserve, Heilongjiang, China, using the line transect method and infrared camera traps from 2022 to 2023. We then analyzed the overlap of their suitable habitats and niches with the aim of gaining insight into the interspecific competition between these two species. We found that the suitable habitat areas for the sable and yellow-throated marten were 55.20 km² and 23.28 km², accounting for 24.86% and 10.48% of the total area of this study, respectively. The overlap between the suitable habitats for the sable and yellow-throated marten was 15.73 km², accounting for 28.5% and 67.6% of their suitable habitat, supporting our Hypothesis 1. The first principal component (Dim1) of the niche explained 35.4% of the overall variability, which is mainly related to the environmental variables “Distance from Settlements” and “Distance from Roads”. Overall, 25.5% of the total variability was explained by the second principal component (Dim2), associated with “Slope” and “Distance from Coniferous and Broadleaved Mixed Forest”. The niches occupied by the sable and yellow-throated marten were both off-center of the environmental background space, with the niches of the sable being larger than those of the yellow-throated marten. Schoener’s D index was 0.56, indicating a high degree of niche overlap between the sable and yellow-throated marten, supporting our Hypothesis 2. Our study is helpful in terms of formulating conservation and management policies for the sable and yellow-throated marten.

This study focuses on the Siberian roe deer population in the Greater Khingan Mountains, Northeast China. The cold temperate forest ecosystem in this area is distinctive. The Siberian roe deer is a crucial ecological indicator species, and its living conditions hold significant importance for ecological balance. From the winter of 2019 to 2022, 269 fecal samples of Siberian roe deer were collected from four protected areas in the northern part of the Greater Khingan Mountains, Heilongjiang Province. These samples were comprehensively analyzed using mitochondrial DNA and microsatellite markers, combined with conservation genetics evaluation methods. The results revealed that 244 individuals were identified in the fecal samples. The results of a Cyt b genetic analysis of the samples indicated that the haplotype and nucleotide diversity were 88.1% and 20.3%, respectively. The evaluation of 14 pairs of microsatellite loci showed that the average number of alleles was 11.2, and the average expected and observed heterozygosity were 0.672 and 0.506, respectively. Therefore, the overall genetic diversity level is high, but some populations of Siberian roe deer are at risk. AMOVA analysis and STRUCTURE Bayesian clustering confirmed the existence of obvious genetic differentiation among the populations. Historical studies have shown that the HZ and SH populations underwent the earliest diffusion events, and the BJC and SL populations also exhibited related signs (HZ: Huzhong Nature Reserve in the Greater Khingan Mountains; SH: Shuanghe National Nature Reserve in Heilongjiang Province; BJC: Heilongjiang Beijicun National Nature Reserve; SL: Songling District in Heilongjiang Province). Mismatch distribution and neutral tests indicated no expansion events or bottleneck effects in the population, and the inbreeding coefficient was positive, suggesting the possibility of inbreeding. The development potential of the population in the future varies among the various local populations. This study supports the biodiversity of Siberian roe deer at the genetic level to save the population and provides important scientific basis and reference for the protection and management of Siberian roe deer.