Curcumin-Hydroxypropyl-β-Cyclodextrin Inclusion Complex Preparation Methods: Effect of Common Solvent Evaporation, Freeze Drying, and pH Shift on Solubility and Stability of Curcumin

The aim of this study was to investigate the drug release behavior from bacterial cellulose (BC). Ibuprofen and propranolol hydrochloride were used as model drugs to represent low and highly water soluble drugs. The drug was loaded into the BC by immersing the partially swollen BC in a solution of drug concentrations ranging from 0.05 to 0.5 mg mL⁻¹ and then drying by two different methods: air-drying and freeze-drying. The results showed that the type of drug and the drying method influenced the drug loading efficiency and drug release behavior. For ibuprofen, high drug loading efficiency was found when loading the drug into BC at low concentration and vice versa for propranolol hydrochloride. The drug-loaded BC prepared by the freeze-drying method showed a sustained release regardless of drug type and drug-loaded amount. The sustained release followed the Higuchi and Korsmeyer–Peppas models. On the other hand, when using the air-drying method, BC loaded with ibuprofen showed immediate release at every drug-loaded amount. However, BC loaded with propranolol hydrochloride showed immediate release at the high drug-loaded amount but showed sustained release at the low drug-loaded amount. The release of drug from a drug-loaded BC prepared by air-drying method tended to follow first-order kinetics. In conclusion, the drug loading concentration and the drying method in the drug-loaded BC preparation influenced the drug release characteristics of the BC-based drug delivery system.

Bacterial cellulose (BC) has been used in the combination with molecularly imprinted polymer (MIP) for controlled-release drug delivery. In the present study, the molecular imprinting was directly performed on BC to avoid the use of synthetic materials for sustained-release of quercetin, which was used as the template molecule. The phase inversion method was successfully used to prepare molecularly imprinted BC (MI-BC). The molecular recognition ability and controlled drug release behavior of MI-BC were then evaluated. MI-BC was found to have approximately 1.6 times higher ability to bind quercetin than the non-imprinted BC (NI-BC) did. The composite membrane containing MI-BC and quercetin (MI-BC-com) delayed and sustained drug release more effectively than the composite membrane containing NI-BC and quercetin (NI-BC-com). MI-BC-com released quercetin approximately two times more slowly than NI-BC-com did at the final hour of the drug release study. The mechanism of quercetin release followed the Higuchi model. Due to the relatively simple method of preparing the drug delivery system without using synthetic MIP, the application of MI-BC may be of great interest in medicine and pharmaceutics.

Curcumin is a naturally occurring substance with various pharmacological activities. It has not been developed as a drug because of its low bioavailability due to its low solubility and absorption. Piperine is a natural enhancer that is popularly used to increase the absorption of curcumin in oral applications; however, it has not been applied for transdermal curcumin delivery. This study aims to develop a transdermal curcumin delivery system using piperine as a skin permeation enhancer in the form of composite double-layer membrane; the upper layer consisted of curcumin and the lower layer consisted of piperine. The amount of curcumin was fixed, but the amount of piperine varied at three levels from 1.96% to 7.41%. The composite membrane had moderate mechanical strength (15–22 MPa) with a good swelling degree (~435%). From an in vitro skin permeation study, piperine had the effect to increase the permeation of curcumin. The permeation rate was related to the amount of piperine. The composite membrane containing piperine at 7.41% could increase the permeation rate of curcumin by about 1.89 times compared with non-piperine contained membrane. Bacterially-derived cellulose containing curcumin and piperine may have the potential for transdermal curcumin delivery in order to improve curcumin’s bioavailability.

The herbal ball has been used as a Thai traditional medicine for relieving many diseases including acne. However, the application process of the herbal ball in practice is complicated and time consuming. The objective of this work was to utilize an herbal ball extract to formulate a gel to reach a more favorable use of the herbal ball for acne treatment. An herbal ball consisting of Andrographis paniculata, Centella asiatica, the Benchalokawichian remedy and the stem bark powder of Hesperethusa crenulata was prepared. The obtained herbal ball was steamed and squeezed to obtain the extract. Gel formulations containing the herbal ball extract at concentrations of 0.1, 1 and 5% w/w were prepared based on a carbomer gel. The herbal ball extract had antioxidant (EC₅₀ = 219.27 ± 36.98 μg/mL) and anti Propionibacterium acnes activities (minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) = 31.25 μg/mL). The 5% w/w gel formulation had antimicrobial activity against P. acnes, showing an inhibition zone value of 10.00 ± 1.00 mm. This indicates that the developed gel formulation has potential for acne treatment. In comparison to the traditional method of herbal ball usage, the application of herbal ball extract in the form of gel should be more convenient to use.

This study presents an efficient high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method for monitoring valproic acid (VPA) level in human plasma. This method is distinguished by its simplicity, cost-effectiveness, and rapid execution, addressing the limitations associated with other advanced analytical techniques like liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), and immunoassays, which are generally complex and costly for routine application. A challenge in analyzing VPA is its non-linear protein binding profile and the absence of a chromophore in its structure, making direct detection difficult. To overcome this, the study developed an efficient HPLC-UV for VPA determination in human plasma, utilizing a simplified and rapid microwave-assisted derivatization process. Due to the lack of a chromophore in VPA structure, this work developed a microwave-assisted derivatization of VPA using phenylhydrazine hydrochloride (PH HCl). The process optimization was achieved at 450 W for 50 s, facilitating effective HPLC-UV detection. The derivatized product was characterized using ¹H nuclear magnetic resonance (NMR) and Fourier transform infrared spectrometer (FT-IR). The derivative, identified as (Z)-N-phenyl-2-propylpentanehydrazonic acid, demonstrated specificity in plasma analysis with no detectable interference. The method exhibited a linear response for VPA concentrations ranging from 30 to 150 μg/mL, with a correlation coefficient exceeding 0.99. Recovery varied between 86.7% and 107%, with a maximum coefficient of variation (CV) of 10.0%. The findings suggest that the microwave-assisted derivatization technique substantially improves the feasibility and cost-effectiveness of HPLC-UV for the analysis of VPA in plasma. This method provides a viable alternative to conventional HPLC methodologies, offering a balance of efficiency and economic practicality for VPA quantification.