Rhizoctonia solani is an important soil-borne fungal pathogen that causes serious diseases on many agricultural crops and vegetables. Here, we report a complete genome assembly of R. solani AG4 (assembly: 45.47 Mb; contig N₅₀: 1.56 Mb), using a combination of Illumina paired-end and PacBio long-read sequencing data. A total of 267 noncoding RNAs and 11,592 genes were predicted, including 109 genes associated with carbohydrate-active enzymes and 2,488 genes involved in host-pathogen interactions. The complete genome of R. solani AG4 represents a valuable base for studying interactions between host plants and pathogenic fungi and to search for potential antimicrobial targets. [ ] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

Larch wood, a prevalent cultivation medium for Ganoderma tsugae, has yet to be scrutinized concerning the differential impacts of sterilized and non-sterilized substrates on the growth and development of this fungus. Our present investigation sought to elucidate these effects in a forest-like environment. After larch wood segments were sun-dried, they were divided into two groups; one group was bagged and autoclaved, while the other group was bagged without any treatment. Subsequently, all segments were inoculated with the G. tsugae strain HLXL1 and ensconced under the canopy of a Pinus koraiensis forest, thereby approximating the conditions of natural growth. Wild G. tsugae was used as the control. Data on agronomic traits, production days, fruiting body yield, and effective constituent content were analyzed. The results indicated no significant differences between sterilized and non-sterilized substrates in terms of agronomic traits. However, the mineral content and bioactive compounds in G. tsugae fruiting bodies significantly differed across various growth stages. The outcomes were optimal for non-sterilized substrates, followed by sterilized substrates, while the wild strains were markedly less effective than the cultivated ones.