Kwak KH, et al. Korean J Orthod. 2016 Sep;46(5):323-330. https://doi.org/10.4041/kjod.2016.46.5.323

Flexible scintillators for digital x-ray image sensors were designed, fabricated and characterized. In these scintillaotrs, terbium-doped gadolinium oxysulfide (Gd2O2S:Tb) scintillator pixels were embedded into a polyethylene (PE) substrate. To evaluate the difference in the spatial resolution according to the pixel size, we designed three scintillators with pixels of different pitch sizes: 50 μm pitch size (P50), 100 μm pitch size (P100) and 200 μm pitch size (P200). Because of the high flexibility and good formability, polyethylene was used as the substrate of the scintillator. To fabricate nickel micromolds with high-aspect-ratio microstructures, two microfabrication techniques were employed: silicon dry-etching using a deep reactive ion etching (DRIE) process and nickel electroforming. The pixelated PE microstructures were fabricated by a hot embossing process. Because the solution-type Gd2O2S:Tb precursor can be handled at room temperature, Gd2O2S:Tb was used as the scintillator material. The measured sensitivities of the P50 and P100 models were, respectively, about 65% and 97% of that of the P200 model. The lower sensitivity values of the models with a small pitch size were due to two factors, such as the different pixel heights and the different fill factors. Because a scintillator with a small pixel size has a low fill factor, the sensitivity of the scintillator decreases as the pixel size decreases. The fill factors of the P50, P100 and P200 models were 36%, 49% and 56.25%, respectively. On the other hand, the spatial resolution of the scintillator increases as the pixel size decreases. Therefore, P50 gave the best spatial resolution among the designed models. The spatial frequency at 10% of the modulation transfer function (MTF) with P50 was 13.5 mm−1, while that with P200 was 10.0 mm−1. The resolution pattern and the tooth x-ray images obtained from a scintillator with a smaller pixel size was also clearer than that obtained from a scintillator with a larger pixel size. PE-based flexible Gd2O2S:Tb scintillators can be utilized directly in flexible x-ray image sensors. (Some figures in this article are in colour only in the electronic version)

Melatonin is a hormone secreted by the pineal gland that is involved in the biorhythm of reproductive activities. The present study investigated the inhibitory effects of melatonin on osteoclastogenesis in RAW 264.7 cells according to changes in V-ATPase and the corresponding inhibition of the MAPK and NFATc1 signaling processes. Methods: the cytotoxic effect of melatonin was investigated by MTT assay. Osteoclast differentiation and gene expression of osteoclast-related factors were confirmed via TRAP staining, pit formation assay, immunofluorescence imaging, western blot, and real-time PCR. Results: melatonin was found to inactivate the p38 and JNK of MAP kinase in RAW264.7 cells treated with RANKL and treated with a combination RANKL and melatonin for 1, 3, and 5 days. The melatonin treatment group showed a reduction in osteoclastogenesis transcription factors and ATP6v0d2 gene expression. Conclusions: melatonin inhibits osteoclast differentiation and cell fusion by inhibiting the expression of Atp6v0d2 through the inactivation of MAPK and NFATc1 signaling in RANKL-stimulated RAW264.7 macrophages. The findings of the present study suggest that melatonin could be a suitable therapy for bone loss and imply a potential role of melatonin in bone health.

Kim SS, et al. Korean J Orthod. 2012 Dec;42(6):307-317. http://dx.doi.org/10.4041/kjod.2012.42.6.307