Yaneselli KM, et al. J Vet Sci. 2018 Jan;19(1):13-20. https://doi.org/10.4142/jvs.2018.19.1.13

Objectives: The aim of this study was to isolate feline dental pulp stem cells (fDPSCs) and characterize their clonogenic and proliferative abilities, as well as their multipotency, immunophenotype and cytogenetic stability. Methods: Dental pulp was isolated by explant culture from two cats <1 year old at post mortem. Their clonogenicity was characterized using a colony-forming unit fibroblast assay, and their proliferative ability was quantified with a doubling time assay in passages 2, 4 and 6 (P₂, P₄ and P₆, respectively). Multipotency was characterized with an in vitro trilineage differentiation assay in P₂, and cells were immunophenotyped in P₄ by flow cytometry. Chromosomic stability was evaluated by cytogenetic analysis in P₂, P₄ and P₆. Results: The fDPSCs displayed spindle and epithelial-like morphologies. Isolated cells showed a marked clonogenic capacity and doubling time was maintained from P₂ to P₆. Trilineage differentiation was obtained in one sample, while the other showed osteogenic and chondrogenic differentiation. Immunophenotypic analysis showed fDPSCs were CD45⁻, CD90⁺ and CD44⁺. Structural and numerical cytogenetic aberrations were observed in P₂–P₄. Conclusions and relevance: In this study, fDPSCs from two cats were isolated by explant culture and immunophenotyped. Cells displayed clonogenic and proliferative ability, and multipotency in vitro, and signs of chromosomic instability were observed. Although a larger study is needed to confirm these results, this is the first report of fDPSC isolation and in vitro characterization.

Stem cell therapy in cat patients needs a high quantity of mesenchymal stromal/stem cells (MSCs) requiring in vitro propagation under culture conditions which may potentially impact cellular characteristics and genetic stability. This study aimed to assess the in vitro characteristics and cytogenetic stability of cat adipose tissue-derived MSCs (cAT-MSCs). For this purpose, morphological features, clonogenic potential, and proliferative capacity of cAT-MSCs were assessed at passages 2 (P₂), P₄, and P₆. Multipotency and immunophenotype were evaluated. Cytogenetic analyses were conducted up to P₆. The cAT-MSCs exhibited a spindle-shaped morphology in early passages. The doubling time increased from 2.5 days at P₂ to 9.4 at P₄ and 10.5 at P₆, accompanied by the observation of nuclear abnormalities such as cluster formation, karyorrhexis, karyolysis, and a decline in the mitotic index at P₄. Cells demonstrated multipotency capacity and were CD45−, CD90+, and CD44+. Metaphase analysis at P₂ and P₄ revealed some indications of structural instability such as gaps, breaks, deletions, duplications, and early chromatid segregation, but these alterations did not show an increase across passages. In conclusion, cAT-MSCs decreased their proliferative capacity after P₄, accompanied by morphological alterations and signs of structural instability.

El uso de plasma rico en plaquetas (PRP) y otros productos derivados de plaquetas (PDP) ha despertado gran interés tanto en medicina humana como veterinaria. Esto se debe a que el PRP puede contribuir a promover la regeneración tisular y la cicatrización de diferentes lesiones de difícil resolución, gracias a que en él se concentran factores de crecimiento. En la Facultad de Veterinaria se brinda el servicio de Medicina Regenerativa Veterinaria (MRV) para el desarrollo de nuevas herramientas terapéuticas a los profesionales veterinarios. El objetivo del presente trabajo fue describir la casuística de solicitud de PDP al servicio de MRV por parte de profesionales en el período de 2018 a 2022. Al recopilar los datos del servicio MRV se encontró que existieron un total de 55 solicitudes del servicio para PDP. Su uso fue principalmente para patologías ortopédicas (73 %), mientras que patologías dermatológicas y odontológicas fueron <10 %. Asimismo, el PRP autólogo fue el más solicitado (93 %), en menor proporción (<5 %) se solicitó fibrina rica en plaquetas (PRF). La especie predominante en las solicitudes fue la canina (56 %), seguida de la equina (38 %) y la felina (con solo un 2 %). La franja etaria de los animales con mayor frecuencia de solicitudes al servicio se ubicó entre 5 y 10 años (44 %). En conclusión, los profesionales veterinarios del Uruguay solicitan el servicio de MRV para la obtención de herramientas complementarias al tratamiento como puede ser el uso de PRP autólogo en su mayoría. La aplicación de PRP en patologías ortopédicas fue lo más solicitado en el período estudiado, asimismo el servicio fue pedido principalmente para caninos y equinos.