We investigated the use of boron-doped diamond (BDD) with different surface morphologies for the enhanced detection of nine different peptides by matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS). For the first time, we compared three different nanostructured BDD film morphologies (Continuous, Nanograss, and Nanotips) with differently terminated surfaces (-H, -O, and -F) to commercially available Ground Steel plates. All these surfaces were evaluated for their effectiveness in detecting the nine different peptides by MALDI-MS. Our results demonstrated that certain nanostructured BDD surfaces exhibited superior performance for the detection of especially hydrophobic peptides (e.g., bradykinin 1–7, substance P, and the renin substrate), with a limit of detection of down to 2.3 pM. Further investigation showed that hydrophobic peptides (e.g., bradykinin 1–7, substance P, and the renin substrate) were effectively detected on hydrogen-terminated BDD surfaces. On the other hand, the highly acidic negatively charged peptide adrenocorticotropic hormone fragment 18–39 was effectively identified on oxygen-/fluorine-terminated BDD surfaces. Furthermore, BDD surfaces reduced sodium adduct contamination significantly.

This paper presents the development and testing of the AP-NURSE monitoring tool aimed to ease the everyday life of patients with Alzheimer’s and Parkinson’s diseases as well as those who take care of such patients. It summarises the activities carried out as part of the international Interreg CE niCE-life project. The team of STU designed, developed, constructed, and tested the AP-NURSE intelligent tool. The paper starts with demographic information on Slovakia. It highlights the fact that population aging is a significant issue, which requires measures to mitigate its consequences at local and European levels. The next part describes the final design of AP-NURSE Home and Care devices and their versions, including details on their hardware and software. A significant part of the paper deals with the testing of AP-NURSE in both laboratory and authentic environments. The laboratory testing was carried out directly at STU. The final testing in an authentic environment was conducted in two social and healthcare centres in Bratislava and in Warsaw. After the activities described in this paper, AP-NURSE reached TRL-6. The AP-NURSE Home platform developed based on the ESP microcontrollers demonstrated the best functionality and has the potential to be used in real practice.