Background: Exploration of composition and chemical characteristics of soil dissolved organic matter (DOM) is significant to understand its biogeochemical role in terrestrial ecosystems. A total of 43 cropped and 16 natural soils (0–20 cm) under four soil types (cinnamon, chernozem, red and paddy soils) across China were collected to investigate the spectral characteristics of DOM using UV–Vis and 3D-EEM spectroscopy.Results: The chernozem soils exhibited the highest aromaticity and humification degree among the four soil types. Ranges of biological index (BIX, 0.53–1.17) and fluorescence index (FI, 1.55–2.10) were found in the investigated DOM, showing joint contribution from allochthonous and autochthonous sources. Higher BIX and FI in the DOM of the paddy and red soils indicated a greater reliance on autochthonous sources for these two soil types. The cropped soils showed no significant differences in chemical characteristics and sources from the natural soils for the cinnamon, chernozem and red soils. UVA (16.2–47.9%) and UVC fulvic-like substances (15.4–40.5%) were the prevailing DOM components, which were highest in the chernozem soils. Additionally, the cropped soils had a higher proportion of humic-like substances than the natural soils in the DOM.Conclusions: Both soil type and land-use strongly affected the chemical characteristics of soil DOM, but only soil type had an impact on the DOM composition for the collected soils. These findings may contribute to the prediction of the biochemical behavior of soil DOM under different soil types and land-uses in terrestrial ecosystems.