Ureolysis-driven microbially induced carbonate precipitation (MICP) is a naturally occurring process facilitated through microbial activities and biogeochemical reactions to produce calcium carbonate (CaCO3) mineral. MICP serves as an alternative ground improvement binder method to conventional technologies which is sustainable, requires low energy for its treatment process, results in a minimal carbon footprint and could offer economic benefits. In the last two decades, MICP has drawn great interest from the scientific community because of its practicality to stabilize granular soils, repair concrete cracks and remediate heavy metals. To obtain successful MICP application, it is vital to understand the conditions that favor its process. This paper, therefore, provides an overview of literature on CaCO3 precipitation mediated by ureolysis-driven MICP and its mechanism. The review includes a discussion on sources of urease enzyme from microorganisms used to induce CaCO3 crystal formation required for implementation of MCIP for ground improvement. Moreover, the key factors that influence the outcome of MICP and bio-engineering testing methods typically used to evaluate MICP performance are also highlighted. Finally, this review also provides insight on the current drawbacks (i.e. ammonium production, scale-up bioprocess and treatment cost) affecting MICP technology and recommendations for future consideration.
1 March 2020 in Biocatalysis and Agricultural Biotechnology
Microbial-induced carbonate precipitation (MICP) is gaining attention as an eco-friendly and sustainable method for concrete crack repair. However, key challenges related to its large-scale implementation, regulatory approval, and integration into existing construction standards remain underexplored. This review examines recent advances in MICP, emphasizing its role in circular economy practices and sustainable building solutions. Traditional synthetic sealants contribute to environmental pollution and have limited long-term durability, highlighting the need for greener alternatives. Global research trends reveal an increasing focus on self-healing materials, biomineralization, and durability enhancement, alongside emerging innovations such as encapsulation technologies, marine applications, and bio-based composites. Unlike previous reviews, this study integrates bibliometric analysis to systematically assess research trends, identify key collaboration networks, and evaluate regulatory challenges that impact MICP adoption. While MICP offers significant advantages, including self-healing capabilities and compatibility with industrial by-products, barriers related to cost, scalability, and policy integration persist. This review identifies critical thematic clusters which include microbial action, sustainability, and engineering applications. This helps to provide actionable insights for researchers, engineers, and policymakers. By fostering interdisciplinary collaboration, MICP has the potential to become a transformative solution for resilient and environmentally sustainable infrastructure.