Manganese (Mn) is involved in plant metabolism as an enzyme cofactor. However, the role of Mn in the formation of volatile compounds in grapes has rarely been studied. To address this gap, this study explored the effect of foliar Mn application on the aroma traits of grapes and wine. Mn nutrient solutions at different concentrations (0 (CK), 300, 1200, and 2400 mg/L) were sprayed on grapevines in 2017 and 2018 and the volatile compounds, odor activity, and sensory features of grapes and wine were investigated. The results showed that Mn application significantly increased Mn content in grape leaves and fruits at harvest. Compared with CK, the total volatile content of grapes was significantly increased by Mn treatment in both years because of the promotion of the accumulation of alcohols and esters. Particularly, 1200 mg/L Mn treatment resulted in a higher sensory score than CK, especially in terms of intensity, duration, and harmony. Multivariate analysis and odor activity values jointly identified eight volatile compounds (ethyl acetate, phenylethyl acetate, and phenylethyl alcohol, etc.) as key odorants that contribute to the floral and fruity flavors of Mn-treated wine. Overall, this study indicated that a moderate concentration of Mn is beneficial for improving the fragrance characteristics of grapes and wines. The results have implications for micronutrient management of grapevines to improve wine flavor quality.

Vitis vinifera L. is a commercially important horticultural plant with abundant microbial resources. However, the impact of grape-associated microbiota on grape quality and flavor has been largely overlooked. We integrated volatomics and microbiomics to explore temporal variations in berry volatiles and microbial diversity of ‘Cabernet Sauvignon’ in Ningxia (NX) and Shanxi (SX), and the correlation between microbial communities and volatiles. A total of 38 and 35 free and bound aroma compounds, respectively, were identified in NX berries and SX berries. For free aroma, these 38 compounds were classified into aldehydes (69%), alcohols (22%), acids (4%), aromatics (4%), terpenes (0.6%), esters (0.37%), and norisoprenoids (0.3%). Similarly, the 35 bound aromas were attributed to aromatics (58%), acids (29%), terpenes (4%), esters (3%), alcohols (2.82%), aldehydes (2.78%), and norisoprenoids (0.4%). Additionally, a total of 616 bacterial genera and 254 fungal genera were detected in all samples from both regions. The results demonstrated that vineyard sites significantly shaped the characteristics of berry volatiles and microbial biogeographic patterns. SX berries exhibited more abundant free aroma and higher microbial diversity than NX berries, with three key taxa (Sphingomonas, Massilia, and Bacillus) identified in the bacterial network. Correlation analysis results highlighted that these key taxa might play an important role in berry-free aroma. This study reveals the crucial role of microbes in shaping grape flavor and uncovers the link between microbial diversity and the regional attributes of grapes and wine.