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Prof. Nelson Lima
Micoteca da Universidade do Minho, CEB-Biological Engineering Centre, University of Minho

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0 Mycology
0 Mycotoxins
0 enviromental biotechnology
0 Microbial Preservation
0 Envrionmental Education

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Review
Published: 21 May 2021 in Mycotoxin Research
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Gallbladder cancer (GBC) is one of the most common sites for biliary tract cancers. It has a worldwide distribution being endemic in South America and Southern Asia. These high GBC rates have previously been linked to the determinants of health such as nutrition, genetics, lifestyle, and environment. Exposure to aflatoxin B1 (AFB1), a human carcinogen, is suggested to be involved with GBC development. This work aims to analyse the interplay of social, lifestyle, and genetic predisposing factors to GBC. AFB1 plays a pivotal role in carcinogenic onset by genetic and epigenetic modifications. AFB1 can induce molecular changes involved in the GBC pathogenesis, such as overexpression of UCHL1 gene, mutagenesis of TP53 gene, abnormal expression of oncogenes BCL-2, and aberrantly methylation of ERBB family receptors. However, a large-scale scientific cooperation is needed to confirm these molecular links through which AFB1 may increase the GBC risk. For that, monitoring AFB1 exposure through AF-albumin and AFB1-lysine will clarify the level of exposure of the population to AFB1 in the GBC hotspot. Further, analyses of AFB1-adduct concentrations in GBC cases (fatal and non-fatal) are needed to understanding if AF contamination can trigger gallbladder cancer.

ACS Style

Jéssica Costa; Nelson Lima; Cledir Santos. An overview on possible links between aflatoxin B1 exposure and gallbladder cancer. Mycotoxin Research 2021, 37, 205 -214.

AMA Style

Jéssica Costa, Nelson Lima, Cledir Santos. An overview on possible links between aflatoxin B1 exposure and gallbladder cancer. Mycotoxin Research. 2021; 37 (3):205-214.

Chicago/Turabian Style

Jéssica Costa; Nelson Lima; Cledir Santos. 2021. "An overview on possible links between aflatoxin B1 exposure and gallbladder cancer." Mycotoxin Research 37, no. 3: 205-214.

Journal article
Published: 20 May 2021 in Water
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Biofilms can be considered the main source of microorganisms in drinking water distribution systems (DWDS). The ecology of a biofilm is dependent on a variety of factors, including the presence of microbial metabolites excreted by its inhabitants. This study reports the effect of the Gram-negative bacteria Methylobacterium oryzae on the idh gene expression levels and patulin production of Penicillium expansum mature biofilms. For this purpose, a RT-qPCR method to quantify idh mRNA levels was applied. In addition, the idh expression levels were compared with the patulin production. The results obtained revealed that the effect of the bacterium on pre-established P. expansum biofilms is dependent on the time of interaction. More mature P. expansum biofilms appear to be more resistant to the inhibitory effect that M. oryzae causes towards idh gene expression and patulin production. A positive trend was observed between the idh expression and patulin production values. The results indicate that M. oryzae affects patulin production by acting at the transcriptional level of the idh gene.

ACS Style

Tiago Afonso; Lúcia Simões; Nelson Lima. Methylobacterium oryzae Influences Isoepoxydon Dehydrogenase Gene Expression and Patulin Production by Penicillium expansum. Water 2021, 13, 1427 .

AMA Style

Tiago Afonso, Lúcia Simões, Nelson Lima. Methylobacterium oryzae Influences Isoepoxydon Dehydrogenase Gene Expression and Patulin Production by Penicillium expansum. Water. 2021; 13 (10):1427.

Chicago/Turabian Style

Tiago Afonso; Lúcia Simões; Nelson Lima. 2021. "Methylobacterium oryzae Influences Isoepoxydon Dehydrogenase Gene Expression and Patulin Production by Penicillium expansum." Water 13, no. 10: 1427.

Research article
Published: 09 January 2021 in Journal of the Science of Food and Agriculture
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BACKGROUND Fermented cocoa beans (Theobroma cacao L.) is a pivotal raw material for chocolate production. A cocktail yeast applied in the cocoa fermentation process can promote the formation of the pleasant metabolites. Saccharomyces, Pichia, and Hanseniaspora have been widely used in fermentation to improve the final product organoleptic profile. Highlighting that fermentation is a critical point for chocolate flavour precursors production. This study aims to evaluate the impact of Pichia kluyveri and Saccharomyces cerevisiae strains as starters cultures on the two cocoa hybrids fermentation FA13 and CEPEC2002. RESULTS During fermentation processes, volatile organic compounds (VOCs) and protein profiles were assessed. Chocolates produced were also assessed regarding the presence of VOCs. Eighty VOCs were identified by gas chromatography coupled to mass spectrometry analysis (HS‐SPME GC‐MS). Mass spectrometry by the MALDI‐TOF technique provided the protein profiles evolution over fermentation and showed they changed with inoculation type (spontaneous vs. inoculated fermentation). Chocolate obtained by FA13 inoculated with S. cerevisiae strain contained higher organics acids amount, being categorised sourer than chocolate produced by spontaneous fermentation of FA13. CEPEC2002 inoculated with S. cerevisiae strain in co‐culture with P. kluyveri strain generated less sour and sweeter chocolate than spontaneous fermentation only. CONCLUSION Chocolates from inoculated assays with starter cultures were more accepted by evaluators. Highlighting that P. kluyveri and S. cerevisiae influence the composition of VOCs. Besides, protein profiles also changed throughout fermentation. Further investigation should be conducted to clarify protein degradation dynamics during inoculated fermentations to defining which of both microbial cultures positively affect the chocolate sensory characteristics.

ACS Style

Igor Moreira; Jéssica Costa; Leonardo Vilela; Nelson Lima; Cledir Santos; Rosane Schwan. Influence of S. cerevisiae and P. kluyveri as starters on chocolate flavour. Journal of the Science of Food and Agriculture 2021, 101, 4409 -4419.

AMA Style

Igor Moreira, Jéssica Costa, Leonardo Vilela, Nelson Lima, Cledir Santos, Rosane Schwan. Influence of S. cerevisiae and P. kluyveri as starters on chocolate flavour. Journal of the Science of Food and Agriculture. 2021; 101 (10):4409-4419.

Chicago/Turabian Style

Igor Moreira; Jéssica Costa; Leonardo Vilela; Nelson Lima; Cledir Santos; Rosane Schwan. 2021. "Influence of S. cerevisiae and P. kluyveri as starters on chocolate flavour." Journal of the Science of Food and Agriculture 101, no. 10: 4409-4419.

Journal article
Published: 30 December 2020 in RILEM Technical Letters
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The cracks in concrete reduce their resistance capacity and allow the entry of harmful agents both for their microstructure and for the reinforcements located inside the structure. Several studies have been done to promote sustainable solutions for this problem. The microbiologically induced calcium carbonate precipitation (MICCP) is an alternative to traditionally used methods and a way to reduce the environmental impact of using more cement and polymers. Most of the biocementation studies to fill cracks or to promote bio self-healing on concrete present bacteria as the microorganisms responsible for the CaCO3 induction process. Fungi are potentially better for the biocementation process because they have more biomass and some develop filaments that can be used as microfibers on materials. Thus, the present work proposes the development of a methodology to analyse the potential use of two urease-positive fungi (Penicillium chrysogenum MUM 9743 and Neurospora crassa MUM 9208) to produce bioconcrete. The microstructure and chemical constituents of biocrystals formed due to MICCP were observed under Scanning Electron Microscopy (SEM). SEM showed fungal mycelia as bio-based fiber in bioconcrete and clusters of probable calcite crystals on and around mycelia. Both fungi were able to promote biocimentation of sand.

ACS Style

Carolina Martuscelli; Célia Soares; Nelson Lima; Aires Camões. Potential of fungi to produce bioconcrete. RILEM Technical Letters 2020, 5, 157 -162.

AMA Style

Carolina Martuscelli, Célia Soares, Nelson Lima, Aires Camões. Potential of fungi to produce bioconcrete. RILEM Technical Letters. 2020; 5 ():157-162.

Chicago/Turabian Style

Carolina Martuscelli; Célia Soares; Nelson Lima; Aires Camões. 2020. "Potential of fungi to produce bioconcrete." RILEM Technical Letters 5, no. : 157-162.

Review article
Published: 13 November 2020 in Research in Microbiology
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Water is indispensable to life and safe and accessible supply must be available to all. The presence of microorganisms is a threat to this commitment. Biofilms are the main reservoir of microorganisms inside water distribution systems and they are extremely ecologically diverse. Filamentous fungi and bacteria can coexist inside these systems forming inter-kingdom biofilms. This review has the goal of summarizing the most relevant and recent reports on the occurrence of filamentous fungi in water distribution systems along with the current knowledge and gaps about filamentous fungal biofilm formation. Special focus is given on fungal-bacterial interactions in water biofilms.

ACS Style

Tiago Barros Afonso; Lúcia Chaves Simões; Nelson Lima. Occurrence of filamentous fungi in drinking water: their role on fungal-bacterial biofilm formation. Research in Microbiology 2020, 172, 103791 .

AMA Style

Tiago Barros Afonso, Lúcia Chaves Simões, Nelson Lima. Occurrence of filamentous fungi in drinking water: their role on fungal-bacterial biofilm formation. Research in Microbiology. 2020; 172 (1):103791.

Chicago/Turabian Style

Tiago Barros Afonso; Lúcia Chaves Simões; Nelson Lima. 2020. "Occurrence of filamentous fungi in drinking water: their role on fungal-bacterial biofilm formation." Research in Microbiology 172, no. 1: 103791.

Journal article
Published: 06 September 2020 in Microorganisms
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Identification of filamentous fungi by conventional phenotypic methods are time-consuming, and a correct identification at the species level is prone to errors. Therefore, a more accurate and faster time-to-results, and cost-effective technique, is required, such as the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). In this study, we describe the development of an in-house spectra library for the identification of filamentous fungi frequently isolated from patients with infections. An in-house spectra library was constructed using 14 reference strains grown in solid medium. Clinical isolates were identified either by the in-house spectra library or the Biotyper commercial library from Bruker Daltonics. Fungal identification was carried following the Biotyper’s established scores: ≤1.699: not reliably identified (NRI); 1.700–1.999: genus-level; ≥2.000: species-level. Clinical isolates were identified, with the in-house library, at species- and genus-level at 88.70% (55) and 3.22% (2), respectively. While 4.80% (3) was NRI and 3.22% (2) was discrepant concerning sequencing. On the contrary, identification up to species and genus-level with the commercial library was 44.44% (16) and 22.22% (8), respectively. NRI and the discrepancy was 30.55% (11) and 2.77% (1), respectively. For the reaming 26 isolates, 16 from Neoscytalidium dimidiatum and 10 from Sporothrix spp., respectively, the absence of spectrum and the specific spectra within the Sporothrix complex in the commercial library resulted in the inability to obtain an identification. In conclusion, the current results advocate the importance that each clinical microbiological laboratory needs to develop an ad hoc library associated with the MALDI-TOF MS fungal identification to overcome the limitations of the available commercial libraries.

ACS Style

Juan C. Gómez-Velásquez; Natalia Loaiza-Díaz; Gilma Norela Hernández; Nelson Lima; Ana C. Mesa-Arango. Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia). Microorganisms 2020, 8, 1362 .

AMA Style

Juan C. Gómez-Velásquez, Natalia Loaiza-Díaz, Gilma Norela Hernández, Nelson Lima, Ana C. Mesa-Arango. Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia). Microorganisms. 2020; 8 (9):1362.

Chicago/Turabian Style

Juan C. Gómez-Velásquez; Natalia Loaiza-Díaz; Gilma Norela Hernández; Nelson Lima; Ana C. Mesa-Arango. 2020. "Development and Validation of an In-House Library for Filamentous Fungi Identification by MALDI-TOF MS in a Clinical Laboratory in Medellin (Colombia)." Microorganisms 8, no. 9: 1362.

Journal article
Published: 20 August 2020 in International Journal of Food Microbiology
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This work aims to provide the first study on the mycobiota present in Chilean pepper Capsicum annuum L. cv. “Cacho de Cabra” throughout the early production stages. Two hundred and forty berry fruits were sampled: 1) at the ripe fruits harvest day; 2) during drying; and 3) smoking processes. A total of 192 strains, encompassing 11 genera and 44 species, were identified through analysis of β-tubulin (benA) gene and internal transcribed spacer of ribosomal DNA (ITS) region. All collection points showed samples with high fungal contamination, but the mycobiota composition varied as a result of different environmental conditions. Alternaria spp. and Fusarium spp. were predominantly isolated from fresh fruits of C. annuum. Penicillium spp. was the most frequent genus in all analysed points. Penicillium brevicompactum and P. crustosum were the most abundant species. Among Aspergillus, A. niger and A. flavus were dominant after the drying phase. In our study, none of the analysed strains of Penicillium (113) and Aspergillus (35) produced Ochratoxin A at detectable levels. The broad characterization of the fungal community of C. annuum carried out in this study, could be a guideline for future mycotoxin analyses performed directly on the pod. Understanding the role and dynamics of mycobiota and its relationship with the toxins present in this substrate, will be useful to establish and improve control measures considering the specificities of each point in the C. annuum production chain.

ACS Style

Jéssica Costa; Rodrigo Rodríguez; Carla Santos; Célia Soares; Nelson Lima; Cledir Santos. Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén. International Journal of Food Microbiology 2020, 334, 108833 .

AMA Style

Jéssica Costa, Rodrigo Rodríguez, Carla Santos, Célia Soares, Nelson Lima, Cledir Santos. Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén. International Journal of Food Microbiology. 2020; 334 ():108833.

Chicago/Turabian Style

Jéssica Costa; Rodrigo Rodríguez; Carla Santos; Célia Soares; Nelson Lima; Cledir Santos. 2020. "Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén." International Journal of Food Microbiology 334, no. : 108833.

Journal article
Published: 31 July 2020 in Journal of Fungi
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Guarana plant is a native of the Amazon region. Due to its high amount of caffeine and tannins, the seed has medicinal and stimulating properties. The guarana industry has grown exponentially in recent years; however, little information is available about associated mycobiota, particularly endophytic fungi. The present study aimed to compare the distribution and diversity of endophytic fungi associated with the leaves and seeds of anthracnose-resistant and susceptible guarana plants produced in Maués and Manaus, Amazonas State, Brazil. A total of 7514 endophytic fungi were isolated on Potato Dextrose Agar, Sabouraud and Czapek media, and grouped into 77 morphological groups. Overall, fungal communities in guarana leaves and seeds were mainly composed by Colletotrichum and Fusarium genera, but also by Chondrostereum, Clonostachys, Curvularia, Hypomontagnella, Lentinus, Neopestalotiopsis, Nigrospora, Peroneutypa, Phyllosticta, Simplicillium and Tinctoporellus. Obtained results indicate that some members of Colletotrichum and Fusarium genera may have experienced dysbiosis during the guarana domestication process, suggesting that some individuals may behave as latent pathogens. The susceptible guarana genotype cultivated in Manaus presented higher fungal diversity. The relative abundance of taxa and diversity among samples suggests that communities are structured by genotype and geographic location. This is the first report of mycobiota in both guarana leaves and seeds.

ACS Style

Carla Santos; Blenda Naara Santos Da Silva; Ana Francisca Tibúrcia Amorim Ferreira E Ferreira; Cledir Santos; Nelson Lima; Jânia Lília Da Silva Bentes. Fungal Endophytic Community Associated with Guarana (Paullinia cupana Var. Sorbilis): Diversity Driver by Genotypes in the Centre of Origin. Journal of Fungi 2020, 6, 123 .

AMA Style

Carla Santos, Blenda Naara Santos Da Silva, Ana Francisca Tibúrcia Amorim Ferreira E Ferreira, Cledir Santos, Nelson Lima, Jânia Lília Da Silva Bentes. Fungal Endophytic Community Associated with Guarana (Paullinia cupana Var. Sorbilis): Diversity Driver by Genotypes in the Centre of Origin. Journal of Fungi. 2020; 6 (3):123.

Chicago/Turabian Style

Carla Santos; Blenda Naara Santos Da Silva; Ana Francisca Tibúrcia Amorim Ferreira E Ferreira; Cledir Santos; Nelson Lima; Jânia Lília Da Silva Bentes. 2020. "Fungal Endophytic Community Associated with Guarana (Paullinia cupana Var. Sorbilis): Diversity Driver by Genotypes in the Centre of Origin." Journal of Fungi 6, no. 3: 123.

Journal article
Published: 04 May 2020 in Construction and Building Materials
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This study aimed to develop a mortar based on hydraulic lime and micro cork granules with antifungal properties. For this purpose, five different compositions were developed, where the amount of cork granules was different and physical and mechanical characterisations were performed. It was observed that the incorporation of micro cork granules causes significant differences in properties such as workability, compressive strength and flexural strength. Some changes in mortars were circumvented by varying the dosage of superplasticizer. The antifungal properties of micro cork granules - added mortars were observed. The results obtained can be considered as a starting point for a transition from current antifungal protections to a new antifungal technique using cork industrial waste materials.

ACS Style

Alexandre Jerónimo; Célia Soares; Barroso Aguiar; Nelson Lima. Hydraulic lime mortars incorporating micro cork granules with antifungal properties. Construction and Building Materials 2020, 255, 119368 .

AMA Style

Alexandre Jerónimo, Célia Soares, Barroso Aguiar, Nelson Lima. Hydraulic lime mortars incorporating micro cork granules with antifungal properties. Construction and Building Materials. 2020; 255 ():119368.

Chicago/Turabian Style

Alexandre Jerónimo; Célia Soares; Barroso Aguiar; Nelson Lima. 2020. "Hydraulic lime mortars incorporating micro cork granules with antifungal properties." Construction and Building Materials 255, no. : 119368.

Journal article
Published: 01 May 2020 in International Journal of Systematic and Evolutionary Microbiology
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This study describes a novel fungal species belonging to the genus Gongronella. During a previous work focusing on metalaxyl degradation by Mucorales strains, two isolates from vineyard soil samples collected in the Alentejo region, south Portugal, were identified as a putative novel species based on combined molecular and MALDI-TOF MS data. This new species is described here using a polyphasic approach that combines morphology, internal transcribed spacer of ribosomal DNA (ITS) and 28S ribosomal DNA (LSU) sequence data analysis and proteomic profiling by MALDI-TOF MS. Phenotypic and molecular data enabled this novel species to be clearly distinguished from other Gongronella species with results of combined ITS+LSU analysis showing that the Gongronella species is related to Gongronella butleri and Gongronella brasiliensis. Therefore, from the results of morphological and molecular analyses, isolates MUM 10.262 and MUM 10.263 seem to represent a new Gongronella species and the name Gongronella eborensis sp. nov. is proposed, with the ex-type strain MUM 10.262 (=CCMI 1100=CBS 128763).

ACS Style

Maria Rosário Martins; Carla Santos; Célia Soares; Cledir Santos; Nelson Lima. Gongronella eborensis sp. nov., from vineyard soil of Alentejo (Portugal). International Journal of Systematic and Evolutionary Microbiology 2020, 70, 3475 -3482.

AMA Style

Maria Rosário Martins, Carla Santos, Célia Soares, Cledir Santos, Nelson Lima. Gongronella eborensis sp. nov., from vineyard soil of Alentejo (Portugal). International Journal of Systematic and Evolutionary Microbiology. 2020; 70 (5):3475-3482.

Chicago/Turabian Style

Maria Rosário Martins; Carla Santos; Célia Soares; Cledir Santos; Nelson Lima. 2020. "Gongronella eborensis sp. nov., from vineyard soil of Alentejo (Portugal)." International Journal of Systematic and Evolutionary Microbiology 70, no. 5: 3475-3482.

Original article
Published: 02 January 2020 in Mycological Progress
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Stereoid fungi are an artificial group with mostly effused-reflexed to stipitate basidiomata, smooth hymenophore, and hyaline spores. From recent surveys in the Brazilian Atlantic Forest, Caatinga, and Cerrado, six specimens of this group had their identity tested with the nrITS and nrLSU sequences. Two of them were nested within the Lopharia s.s. clade and represent a new species L. erubescens, characterized by the dextrinoid reaction of the cystidia, and small basidia and spores. The other four were initially identified as Hjortstamia amethystea, but nested in the Phlebiopsis clade. Thus, we proposed the new combination, Phlebiopsis amethystea. We also provide keys to neotropical Lopharia s.l. and Phlebiopsis s.l. and allied species.

ACS Style

Vitor Xavier De Lima; Carla De Sousa Lira; Renata Dos Santos Chikowski; Carla Santos; Nelson Lima; Tatiana Baptista Gibertoni. Additions to neotropical stereoid fungi (Polyporales, Basidiomycota): one new species of Lopharia and one new combination in Phlebiopsis. Mycological Progress 2020, 19, 31 -40.

AMA Style

Vitor Xavier De Lima, Carla De Sousa Lira, Renata Dos Santos Chikowski, Carla Santos, Nelson Lima, Tatiana Baptista Gibertoni. Additions to neotropical stereoid fungi (Polyporales, Basidiomycota): one new species of Lopharia and one new combination in Phlebiopsis. Mycological Progress. 2020; 19 (1):31-40.

Chicago/Turabian Style

Vitor Xavier De Lima; Carla De Sousa Lira; Renata Dos Santos Chikowski; Carla Santos; Nelson Lima; Tatiana Baptista Gibertoni. 2020. "Additions to neotropical stereoid fungi (Polyporales, Basidiomycota): one new species of Lopharia and one new combination in Phlebiopsis." Mycological Progress 19, no. 1: 31-40.

Short communications
Published: 23 December 2019 in Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology
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Our study included a total of 36 Fomes fomentarius specimens obtained from Portugal, Spain, Italy, Greece and the Czech Republic. After multiple sequence alignment analysis we observed that samples collected in Southern Europe belong to the lineage B with the exception of one specimen from northern Greece.

ACS Style

Kateřina Náplavová; Ján Gáper; Svetlana Gáperová; Terézia Beck; Peter Pristaš; Célia Soares; Nelson Lima. Genetic and plant host differences of Fomes fomentarius in selected parts of Southern Europe. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 2019, 154, 125 -127.

AMA Style

Kateřina Náplavová, Ján Gáper, Svetlana Gáperová, Terézia Beck, Peter Pristaš, Célia Soares, Nelson Lima. Genetic and plant host differences of Fomes fomentarius in selected parts of Southern Europe. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 2019; 154 (1):125-127.

Chicago/Turabian Style

Kateřina Náplavová; Ján Gáper; Svetlana Gáperová; Terézia Beck; Peter Pristaš; Célia Soares; Nelson Lima. 2019. "Genetic and plant host differences of Fomes fomentarius in selected parts of Southern Europe." Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 154, no. 1: 125-127.

Case report
Published: 21 December 2019 in Microorganisms
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Sporotrichosis is a globally distributed subcutaneous fungal infection caused by dimorphic fungi belonging to the Sporothrix species complex that affects the skin of limbs predominantly, but not exclusively. A rare case of ocular sporotrichosis in an immunocompetent Brazilian patient from the countryside of Rio de Janeiro State is reported. A 68-year-old woman presented with a subconjunctival infiltrative lesion in the right eye with pre-auricular lymphadenopathy of onset 4 months ago that evolved to suppurative nodular lesions on the eyelids. Conjunctival secretion was evaluated by histopathological examination and inoculated on Sabouraud Dextrose Agar (SDA). Histopathology showed oval bodies within giant cells and other mononucleated histiocytes. Fungus grown on SDA was identified as Sporothrix sp. by morphological observations. The isolated strain was finally identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) associated with an in-house database enriched with reference Sporothrix complex spectra. The strain presented a MALDI spectrum with the ion peaks of the molecular mass profile of S. brasiliensis. The patient was adequately treated with amphotericin B subsequently replaced by itraconazole. Due to scars left by the suppurative process, the patient presented poor final visual acuity. The present work presents an overview of ocular sporotrichosis and discusses the diagnostic difficulty that can lead to visual sequelae in these cases.

ACS Style

Aline M. F. Matos; Lucas M. Moreira; Bianca F. Barczewski; Lucas X. De Matos; Jordane B. V. De Oliveira; Maria Ines F. Pimentel; Rodrigo Almeida-Paes; Murilo G. Oliveira; Tatiana C. A. Pinto; Nelson Lima; Magnum De O. Matos; Louise G. De M. E Costa; Cledir Santos; Manoel Marques Evangelista Oliveira. Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient. Microorganisms 2019, 8, 22 .

AMA Style

Aline M. F. Matos, Lucas M. Moreira, Bianca F. Barczewski, Lucas X. De Matos, Jordane B. V. De Oliveira, Maria Ines F. Pimentel, Rodrigo Almeida-Paes, Murilo G. Oliveira, Tatiana C. A. Pinto, Nelson Lima, Magnum De O. Matos, Louise G. De M. E Costa, Cledir Santos, Manoel Marques Evangelista Oliveira. Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient. Microorganisms. 2019; 8 (1):22.

Chicago/Turabian Style

Aline M. F. Matos; Lucas M. Moreira; Bianca F. Barczewski; Lucas X. De Matos; Jordane B. V. De Oliveira; Maria Ines F. Pimentel; Rodrigo Almeida-Paes; Murilo G. Oliveira; Tatiana C. A. Pinto; Nelson Lima; Magnum De O. Matos; Louise G. De M. E Costa; Cledir Santos; Manoel Marques Evangelista Oliveira. 2019. "Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient." Microorganisms 8, no. 1: 22.

Articles
Published: 26 November 2019 in Biofouling
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The main focus so far in the study of biofilm formation in drinking water has been bacteria. Studies on biofilm formation involving filamentous fungi are, therefore, scarce. This study aimed to assess and characterize the ability of these microorganisms to interact with bacteria whilst forming inter-kingdom biofilms. Biofilms were analysed in terms of total biomass, metabolic activity, bacterial colony forming units and morphology by epifluorescence microscopy. The quantitative methods revealed that biofilm mass increased over time for both single and inter-kingdom biofilms, while specific metabolic activity decreased, in general, along the time points evaluated. Microscopic data visually confirmed the biofilm mass increase over time. This study shows that fungal stage development is important in the first 24 h of biofilm formation. Inter-kingdom biofilm formation is microorganism dependent and inter-kingdom biofilms may provide an advantage to the opportunistic bacterium Acinetobacter calcoaceticus to replicate and proliferate when compared with Methylobacterium oryzae.

ACS Style

Tiago Afonso; Lúcia Simões; Nelson Lima. In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system. Biofouling 2019, 35, 1041 -1054.

AMA Style

Tiago Afonso, Lúcia Simões, Nelson Lima. In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system. Biofouling. 2019; 35 (10):1041-1054.

Chicago/Turabian Style

Tiago Afonso; Lúcia Simões; Nelson Lima. 2019. "In vitro assessment of inter-kingdom biofilm formation by bacteria and filamentous fungi isolated from a drinking water distribution system." Biofouling 35, no. 10: 1041-1054.

Original article
Published: 18 November 2019 in Annals of Microbiology
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Purpose The diversity of lactic acid bacteria (LAB) in silages produced in warm climate countries is not well known. This study aimed to identify and characterise the metabolic and genotypic aspects of autochthonous LAB isolated from corn silage produced in the state of Minas Gerais, Brazil. Methods Eighty-eight LAB were isolated. To evaluate their performance at the strain level, all isolates were distinguished among strains using random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) and repetitive extragenic palindromic PCR (REP-PCR) techniques. The organic acid and ethanol production were determined by high-performance liquid chromatography (HPLC). Result The fingerprints obtained by RAPD-PCR with a M13 primer were more discriminatory than those obtained with the REP-PCR technique using a (GACA)4 primer. Moreover, 28 representative isolates were identified as Lactobacillus acidophilus, L. buchneri, L. casei, L. diolivorans, L. hilgardii, L. paracasei, L. parafarraginis, L. plantarum, L. rhamnosus, L. zeae and Pediococcus acidilactici. Different fingerprinting profiles between isolates within the same species were observed. However, some strains isolated from different silages showed the same band profile, thus suggesting the presence of clusters with high similar fingerprints in silages from various regions. Conclusion A variation in LAB diversity was observed in the silages of the evaluated regions, with L. rhamnosus and L. buchneri showing the highest distribution. Differences in organic acid production were observed among the strains belonging to the same species. This research contributes to a better understanding of the LAB community present in corn silage produced in warm climates. These strains will be studied as potential silage starters.

ACS Style

Andréia Santos; Carla Luiza Da Silva Ávila; Célia Soares; Beatriz Ferreira Carvalho; Rosane Schwan; Nelson Lima. Lactic acid bacteria diversity in corn silage produced in Minas Gerais (Brazil). Annals of Microbiology 2019, 69, 1445 -1459.

AMA Style

Andréia Santos, Carla Luiza Da Silva Ávila, Célia Soares, Beatriz Ferreira Carvalho, Rosane Schwan, Nelson Lima. Lactic acid bacteria diversity in corn silage produced in Minas Gerais (Brazil). Annals of Microbiology. 2019; 69 (13):1445-1459.

Chicago/Turabian Style

Andréia Santos; Carla Luiza Da Silva Ávila; Célia Soares; Beatriz Ferreira Carvalho; Rosane Schwan; Nelson Lima. 2019. "Lactic acid bacteria diversity in corn silage produced in Minas Gerais (Brazil)." Annals of Microbiology 69, no. 13: 1445-1459.

Journal article
Published: 01 November 2019 in Water Research
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Filamentous fungi (ff) are consistently recognized as drinking water (DW) inhabitants, typically harboured in biofilms. Their sessile behaviour is still poorly understood. This study aimed the evaluation of the influence of several abiotic factors (substratum, hydrodynamic conditions and nutrient availability) on biofilm formation by Penicillium brevicompactum and P. expansum isolated from DW. Fungal adhesion was quantified on high density polyethylene (HDPE) and polyvinyl chloride (PVC) surfaces using synthetic tap water (STW) and R2A broth, under stagnant or agitated (25 and 150 rpm) conditions. Fungal spore numbers were assessed after staining with Calcofluor White MR2 and epifluorescence microscopy. The surface charge and physicochemical properties of spores were characterized for a mechanistic understanding on the adhesion process. The adhesion kinetics of spores was represented accurately by the Logistic model, in which adhesion increased with time until a maximum level attained before spore germination (8 h after incubation). In general, P. brevicompactum demonstrated to adhere in a higher extent than P. expansum (12 × 104 spores/cm2vs 1.7 × 104 spores/cm2). Moreover, fungal adhesion was potentiated under stagnation and using R2A broth. HDPE and PVC allowed spore adhesion at similar extents. Adhesion predictions based on the extended Derjaguin, Landau, Verwey and Overbeek (XDLVO) theory corroborated the experimental results, highlighting the role of physicochemical surface properties on the adhesion of spores. These results allowed to refine a model for ff biofilm formation. The overall results help to understand key aspects determining the presence of P. brevicompactum and P. expansum biofilms in DW, where stagnant conditions and the presence of nutrients should be avoided to prevent ff biofilm formation.

ACS Style

Susana Fernandes; Lúcia Simões; Nelson Lima; Manuel Simões. Adhesion of filamentous fungi isolated from drinking water under different process conditions. Water Research 2019, 164, 114951 .

AMA Style

Susana Fernandes, Lúcia Simões, Nelson Lima, Manuel Simões. Adhesion of filamentous fungi isolated from drinking water under different process conditions. Water Research. 2019; 164 ():114951.

Chicago/Turabian Style

Susana Fernandes; Lúcia Simões; Nelson Lima; Manuel Simões. 2019. "Adhesion of filamentous fungi isolated from drinking water under different process conditions." Water Research 164, no. : 114951.

Journal article
Published: 01 September 2019 in Microorganisms
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Within the Neoscytalidium genus, N. dimidiatum, N. oculus, N. orchidacearum, and N. novaehollandiae have been recognized. Although these species are frequently found in soil, N. dimidiatum has been identified as an etiologic agent of onychomycosis or dermatomycosis, and N. oculus has been identified as an etiologic agent of an ocular lesion. All these species can be cultured in vitro, but their morphological identification by macroscopic and microscopic traits is difficult and imprecise due to their similarity. In this study, 34 isolates of Neoscytalidium spp. from 32 onychomycosis and two dermatomycosis cases in Medellin (Colombia) were identified at the species level using sequencing of the ITS1+5.8S+ITS2 nuclear rDNA region and MALDI-TOF mass spectrometry (MS). Neoscytalidium dimidiatum strain MUM 17.21 was used to construct the reference spectrum in the in-house library to identify the clinical isolates by MALDI-TOF MS. Additionally, N. dimidiatum PPC-216 and PLAB-055 strains were used to validate the in-house constructed reference spectra. Although four groups were observed in the dendrogram obtained from the proteins of each isolate profile, MALDI-TOF MS and sequencing results are in accordance, since all isolates were identified as N. dimidiatum.

ACS Style

Sindy V. Flórez-Muñoz; Juan C. Gómez-Velásquez; Natalia Loaiza-Díaz; Célia Soares; Carla Santos; Nelson Lima; Ana C. Mesa-Arango. ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated from Onychomycosis and Dermatomycosis Cases in Medellin (Colombia). Microorganisms 2019, 7, 306 .

AMA Style

Sindy V. Flórez-Muñoz, Juan C. Gómez-Velásquez, Natalia Loaiza-Díaz, Célia Soares, Carla Santos, Nelson Lima, Ana C. Mesa-Arango. ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated from Onychomycosis and Dermatomycosis Cases in Medellin (Colombia). Microorganisms. 2019; 7 (9):306.

Chicago/Turabian Style

Sindy V. Flórez-Muñoz; Juan C. Gómez-Velásquez; Natalia Loaiza-Díaz; Célia Soares; Carla Santos; Nelson Lima; Ana C. Mesa-Arango. 2019. "ITS rDNA Gene Analysis Versus MALDI-TOF MS For Identification of Neoscytalidium dimidiatum Isolated from Onychomycosis and Dermatomycosis Cases in Medellin (Colombia)." Microorganisms 7, no. 9: 306.

Journal article
Published: 25 August 2019 in Microorganisms
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This study aims to evaluate the effect of freeze-drying and long-term storage on the biotechnological potential of Aspergillus section Nigri strains. Twelve selected strains were freeze-dried and aged by accelerated storage, at 37 °C in the dark, for 2 and 4 weeks. To assess possible changes as a consequence of the ageing in the freeze-drying ampoules, morphological characteristics, mycotoxins and enzymes production, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALTI-TOF MS) spectra, and M13 phage probe fingerprinting were used as part of a polyphasic approach. Phenotypical changes were observed; nevertheless, they did not substantially affect the potential biotechnological use of these strains. The activity of hydrolytic enzymes (protease, carboxymethylcellulase, xylanase, pectinase and mannanase) was maintained or increased after freeze-drying. MALDI-TOF MS data originated spectra that grouped, for the majority of samples, according to strain independently of preservation time point. M13 profiles revealed the presence of some genetic polymorphisms after preservation. However, the three studied times still clustered for more than 50% of strains. Our results show that the studied strains maintain their biotechnological potential after preservation, with minimal phenotypic alterations. These findings provide evidence that freeze-drying preservation is a suitable option to preserve biotechnologically relevant aspergilli strains from section Nigri, and one should consider that the observed effects might be species/strain-dependent.

ACS Style

Rodrigo Rodriguez; Carla Santos; Marta F. Simões; Célia Soares; Cledir Santos; Nelson Lima. Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains. Microorganisms 2019, 7, 291 .

AMA Style

Rodrigo Rodriguez, Carla Santos, Marta F. Simões, Célia Soares, Cledir Santos, Nelson Lima. Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains. Microorganisms. 2019; 7 (9):291.

Chicago/Turabian Style

Rodrigo Rodriguez; Carla Santos; Marta F. Simões; Célia Soares; Cledir Santos; Nelson Lima. 2019. "Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains." Microorganisms 7, no. 9: 291.

Journal article
Published: 25 March 2019 in Applied Surface Science
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The use of lime-based coatings came into disuse with the appearance of cement, a stronger and easier to apply binder. However, cement mortars have proven to be rigid to replace lime mortars. In rehabilitation the use of lime-based mortars has been more frequent. With the increase of this type of works it is expected to increase the application of this type of coatings. Fungi develop on the surface, inside pores and cracks of walls and ceilings and they play a very important role in the degradation of the quality of the buildings and their interior environment. Inside the buildings, the growth of fungi in wet areas, in addition to hygiene and aesthetic problems can also cause respiratory allergy problems. This study aimed to develop hydraulic lime mortar incorporating nano additives with antifungal properties. The main objective of this study was to verify the development of fungi in mortars added with nano TiO2. The physical and mechanical characteristics were also verified. For this, four different compositions were developed. The amount of nano TiO2 was varied. It has been possible to observe that the incorporation of nano TiO2 causes significant differences in properties such as workability, compressive and flexural strength. Some changes observed in the mortars were compensated by varying the dosage of the superplasticizer. The antifungal properties of nano additivated mortars with TiO2 were verified. The results obtained could be a starting point for a transition from the current antifungal protections to new antifungal protections using nano additives. This transition of antifungal protection will allow an improvement of the quality of the constructions, the health of the users as well as the environmental quality.

ACS Style

Alexandre Jerónimo; Aires Camões; Barroso Aguiar; Nelson Lima. Hydraulic lime mortars with antifungal properties. Applied Surface Science 2019, 483, 1192 -1198.

AMA Style

Alexandre Jerónimo, Aires Camões, Barroso Aguiar, Nelson Lima. Hydraulic lime mortars with antifungal properties. Applied Surface Science. 2019; 483 ():1192-1198.

Chicago/Turabian Style

Alexandre Jerónimo; Aires Camões; Barroso Aguiar; Nelson Lima. 2019. "Hydraulic lime mortars with antifungal properties." Applied Surface Science 483, no. : 1192-1198.

Opinion article
Published: 27 February 2019 in Frontiers in Microbiology
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Fungal Community Ecology Using MALDI-TOF MS Demands Curated Mass Spectral Databases

ACS Style

Matheus Sanitá Lima; Rosymar Coutinho de Lucas; Nelson Lima; Maria De Lourdes Teixeira De Moraes Polizeli; Cledir Santos. Fungal Community Ecology Using MALDI-TOF MS Demands Curated Mass Spectral Databases. Frontiers in Microbiology 2019, 10, 315 .

AMA Style

Matheus Sanitá Lima, Rosymar Coutinho de Lucas, Nelson Lima, Maria De Lourdes Teixeira De Moraes Polizeli, Cledir Santos. Fungal Community Ecology Using MALDI-TOF MS Demands Curated Mass Spectral Databases. Frontiers in Microbiology. 2019; 10 ():315.

Chicago/Turabian Style

Matheus Sanitá Lima; Rosymar Coutinho de Lucas; Nelson Lima; Maria De Lourdes Teixeira De Moraes Polizeli; Cledir Santos. 2019. "Fungal Community Ecology Using MALDI-TOF MS Demands Curated Mass Spectral Databases." Frontiers in Microbiology 10, no. : 315.