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Vitellogenin is an essential protein involved in ovary maturation in many animals. Detection of this protein correlated with reproductive capacity may be important if carried out on marine organisms such as the red spiny lobster Palinurus elephas, a crustacean economically important crop from wild fish catches. Moreover, in recent years, vitellogenin has assumed an important role as a possible biomarker of marine environmental pollution, as its expression levels can be influenced by the presence of similar oestrogen pollutants and affect the reproductive sphere of marine organisms such as crustaceans. The P. elephas vitellogenin protein and its coding gene have never been isolated, so there is little information about its presence in this lobster. The aim of the present study was to develop a molecular strategy to create, for the first time, an antibody for the detection and quantization of vitellogenin in P. elephas.
Faustina B Cannea; Cristina Follesa; Cristina Porcu; Rossano Rossino; Alessandra Olianas; Antonio Rescigno; Alessandra Padiglia. Development of the first antibody targeting vitellogenin using the Palinurus elephas mRNA molecular pathway. 2021, 1 .
AMA StyleFaustina B Cannea, Cristina Follesa, Cristina Porcu, Rossano Rossino, Alessandra Olianas, Antonio Rescigno, Alessandra Padiglia. Development of the first antibody targeting vitellogenin using the Palinurus elephas mRNA molecular pathway. . 2021; ():1.
Chicago/Turabian StyleFaustina B Cannea; Cristina Follesa; Cristina Porcu; Rossano Rossino; Alessandra Olianas; Antonio Rescigno; Alessandra Padiglia. 2021. "Development of the first antibody targeting vitellogenin using the Palinurus elephas mRNA molecular pathway." , no. : 1.
: The process of melanogenesis, that takes place in the melanocytes of the epidermis, leads to hyperpigmentation. The biosynthetic pathway for production of melanin involves the enzyme tyrosinase that has been an attractive target for cosmaceutical research. Numerous synthetic, semisynthetic and natural, especially plant-based, inhibitors of tyrosinase have been reported in the literature. In plants, the secondary metabolites like flavonoids, chalcones, stilbenes, tannins, hydroquinone and kojic acid, etc... have been shown to possess the anti-tyrosinase activity. In the current review, we have covered the progress in this sphere that would be useful for not only further mechanistic investigations but also for the optimization of the structure of the metabolites for improved activity and selectivity. Thus the review presents a comprehensive report on tyrosinase inhibitors of plant origin reported in the extract form or as isolated compounds. Huge gap has been found between research and industry due to inconsistent pursual of the potent plant based extracts. There is a need to completely evaluate the extracts for structure optimization using molecular docking and evaluation of the safety inorder to benefit the industry with non toxic biological friendly products through invivo and exvivo optimization.
Rabia Riaz; Paolo Zucca; Antonio Rescigno; Stefania Peddio; Rahman Shah Zaib Saleem; Salma Batool. Plants as a Promising Reservoir of Tyrosinase Inhibitors. Mini-Reviews in Organic Chemistry 2020, 17, 1 -23.
AMA StyleRabia Riaz, Paolo Zucca, Antonio Rescigno, Stefania Peddio, Rahman Shah Zaib Saleem, Salma Batool. Plants as a Promising Reservoir of Tyrosinase Inhibitors. Mini-Reviews in Organic Chemistry. 2020; 17 ():1-23.
Chicago/Turabian StyleRabia Riaz; Paolo Zucca; Antonio Rescigno; Stefania Peddio; Rahman Shah Zaib Saleem; Salma Batool. 2020. "Plants as a Promising Reservoir of Tyrosinase Inhibitors." Mini-Reviews in Organic Chemistry 17, no. : 1-23.
Polyphenol oxidase (PPO, E.C. 1.14.18.1) is a nearly ubiquitous enzyme that is widely distributed among organisms. Despite its widespread distribution, the role of PPO in plants has not been thoroughly elucidated. In this study, we report for the absence of PPO in Cynomorium coccineum, a holoparasitic plant adapted to withstand unfavorable climatic conditions, growing in Mediterranean countries and amply used in traditional medicine. The lack of PPO has been demonstrated by the absence of enzymatic activity with various substrates, by the lack of immunohistochemical detection of the enzyme, and by the absence of the PPO gene and, consequently, its expression. The results obtained in our work allow us to exclude the presence of the PPO activity (both latent and mature forms of the enzyme), as well as of one or more genes coding for PPO in C. coccineum. Finally, we discuss the possible significance of PPO deficiency in parasitic plants adapted to abiotic stress.
Alessandra Padiglia; Paolo Zucca; Faustina B. Cannea; Andrea Diana; Cristina Maxia; Daniela Murtas; Antonio Rescigno. Absence of Polyphenol Oxidase in Cynomorium coccineum, a Widespread Holoparasitic Plant. Plants 2020, 9, 964 .
AMA StyleAlessandra Padiglia, Paolo Zucca, Faustina B. Cannea, Andrea Diana, Cristina Maxia, Daniela Murtas, Antonio Rescigno. Absence of Polyphenol Oxidase in Cynomorium coccineum, a Widespread Holoparasitic Plant. Plants. 2020; 9 (8):964.
Chicago/Turabian StyleAlessandra Padiglia; Paolo Zucca; Faustina B. Cannea; Andrea Diana; Cristina Maxia; Daniela Murtas; Antonio Rescigno. 2020. "Absence of Polyphenol Oxidase in Cynomorium coccineum, a Widespread Holoparasitic Plant." Plants 9, no. 8: 964.
Weeds thrive in agricultural environments. However, in certain areas of the world, they are consumed by humans as food, and they can represent a source of valuable active ingredients of ethnomedical interest. In this study, three North African parasitic plants of the Orobanchaceae family,...
Imen Ben Attia; Paolo Zucca; Flaminia Cesare Marincola; Mariella Nieddu; Alessandra Piras; Antonella Rosa; Antonio Rescigno; Mohamed Chaieb. Evaluation of the Antioxidant and Cytotoxic Activities on Cancer Cell Line of Extracts of Parasitic Plants Harvested in Tunisia. Polish Journal of Food and Nutrition Sciences 2020, 253 -263.
AMA StyleImen Ben Attia, Paolo Zucca, Flaminia Cesare Marincola, Mariella Nieddu, Alessandra Piras, Antonella Rosa, Antonio Rescigno, Mohamed Chaieb. Evaluation of the Antioxidant and Cytotoxic Activities on Cancer Cell Line of Extracts of Parasitic Plants Harvested in Tunisia. Polish Journal of Food and Nutrition Sciences. 2020; ():253-263.
Chicago/Turabian StyleImen Ben Attia; Paolo Zucca; Flaminia Cesare Marincola; Mariella Nieddu; Alessandra Piras; Antonella Rosa; Antonio Rescigno; Mohamed Chaieb. 2020. "Evaluation of the Antioxidant and Cytotoxic Activities on Cancer Cell Line of Extracts of Parasitic Plants Harvested in Tunisia." Polish Journal of Food and Nutrition Sciences , no. : 253-263.
Sisymbrium officinale (hedge mustard and formerly called Erysimum officinale) is a common plant in wild lands of Europe and Africa. It is also cultivated for its seeds and leaves to be used in salad or mustard. Sisymbrium officinale is useful not only in culinary preparations, but it also seems to possess interesting therapeutic properties, especially for throat diseases such as aphonia and hoarseness. For this reason, it is commonly called “herb of singers” (in Italian, “Erba dei cantanti”). Indeed a cup of Sisymbrium officinale infusion is frequently consumed by singers before artistic performance, even if its beneficial ability still needs to be scientifically demonstrated. Some preliminary data can be analyzed, but new efforts and resources should be devoted to study and investigate a plant with valuable therapeutic potential. This review summarizes the data available for Sisymbrium officinale.
Maira Zorzan; Paolo Zucca; Daniela Collazuol; Stefania Peddio; Antonio Rescigno; Raffaele Pezzani. Sisymbrium officinale, the Plant of Singers: A Review of Its Properties and Uses. Planta Medica 2020, 86, 307 -311.
AMA StyleMaira Zorzan, Paolo Zucca, Daniela Collazuol, Stefania Peddio, Antonio Rescigno, Raffaele Pezzani. Sisymbrium officinale, the Plant of Singers: A Review of Its Properties and Uses. Planta Medica. 2020; 86 (5):307-311.
Chicago/Turabian StyleMaira Zorzan; Paolo Zucca; Daniela Collazuol; Stefania Peddio; Antonio Rescigno; Raffaele Pezzani. 2020. "Sisymbrium officinale, the Plant of Singers: A Review of Its Properties and Uses." Planta Medica 86, no. 5: 307-311.
Avocado and soybean unsaponifiables (ASU) constitute vegetable extracts made from fruits and seeds of avocado and soybean oil. Characterized by its potent anti-inflammatory effects, this ASU mixture is recommended to act as an adjuvant treatment for osteoarthritic pain and slow-acting symptomatic treatment of hip and knee osteoarthritis; autoimmune diseases; diffuse scleroderma and scleroderma-like states (e.g., morphea, sclerodactyly, scleroderma in bands). Besides, it was reported that it can improve the mood and quality of life of postmenopausal women in reducing menopause-related symptoms. This article aims to summarize the studies on biological effects of the avocado–soybean unsaponifiable, its chemical composition, pharmacotherapy as well as applications in autoimmune, osteoarticular and menopausal disorders. Finally, we will also discuss on its safety, toxicological and regulatory practices.
Bahare Salehi; Antonio Rescigno; Tinuccia Dettori; Daniela Calina; Anca Oana Docea; Laxman Singh; Fatma Cebeci; Beraat Özçelik; Mohammed Bhia; Amirreza Dowlati Beirami; Javad Sharifi-Rad; Farukh Sharopov; William C. Cho; Natália Martins. Avocado–Soybean Unsaponifiables: A Panoply of Potentialities to Be Exploited. Biomolecules 2020, 10, 130 .
AMA StyleBahare Salehi, Antonio Rescigno, Tinuccia Dettori, Daniela Calina, Anca Oana Docea, Laxman Singh, Fatma Cebeci, Beraat Özçelik, Mohammed Bhia, Amirreza Dowlati Beirami, Javad Sharifi-Rad, Farukh Sharopov, William C. Cho, Natália Martins. Avocado–Soybean Unsaponifiables: A Panoply of Potentialities to Be Exploited. Biomolecules. 2020; 10 (1):130.
Chicago/Turabian StyleBahare Salehi; Antonio Rescigno; Tinuccia Dettori; Daniela Calina; Anca Oana Docea; Laxman Singh; Fatma Cebeci; Beraat Özçelik; Mohammed Bhia; Amirreza Dowlati Beirami; Javad Sharifi-Rad; Farukh Sharopov; William C. Cho; Natália Martins. 2020. "Avocado–Soybean Unsaponifiables: A Panoply of Potentialities to Be Exploited." Biomolecules 10, no. 1: 130.
The botanical identity of the ancient vernacular cynomorium does not correspond to the modern scientific genus while it is not clear how many species of hipocistis (Cytinus sp.) were differentiated by the ancient physicians and whether Cynomorium coccineum was subsumed. The early history of therapeutic uses related to the herbal drugs derived from these parasitic taxa is therefore not easily accessible. Cynomorium coccineum became an important pharmaceutical commodity after the Siege of Malta but its importance decreased in the 18th century and now is considered obsolete. We compare the morphological, ecological and therapeutic information of Cynomorium and other parasitizing plant taxa across the past 2000 years and contextualize their uses with the pharmacological properties of their principal metabolites focusing on the raise and fall of C. coccineum as a medicine. The therapeutic uses of C. coccineum, the Maltese mushroom, seem to become clearly traceable since the Canon of Medicine by Avicenna. Styptic and astringent drugs such as Cynomorium, Cytinus but also gall apples and many others have been selected for their protein-linking capacity leading to the formation of a protective layer on the mucous membranes, which can be used to reduce the secretion of water and electrolytes in case of diarrhoea, dysentery and external bleedings. Whether C. coccineum is effective as a systemically applied anti-haemorrhagic drug is questionable. It appears that the vernacular cynomorium of the ancients corresponds to an edible Orobanche sp. while it remains doubtful whether the vernacular hipocistis was next to Cytinus sp. also applied to C. coccineum as evidence of C. coccineum parasitizing Cistus sp. is scarce. The isolation of gallic acid used as a styptic and the increasing availability of chemical styptics in the 18th century together with the availability of effective alternative anti-diarrhoeic drugs with a more reliable supply very probably led to the decline of the importance of the Maltese mushroom in pharmacy during the 18th century. The effectiveness of gallic acid as a systemic anti-haemorrhagic remains uncertain.
Marco Leonti; Sidonie Bellot; Paolo Zucca; Antonio Rescigno. Astringent drugs for bleedings and diarrhoea: The history of Cynomorium coccineum (Maltese Mushroom). Journal of Ethnopharmacology 2019, 249, 112368 .
AMA StyleMarco Leonti, Sidonie Bellot, Paolo Zucca, Antonio Rescigno. Astringent drugs for bleedings and diarrhoea: The history of Cynomorium coccineum (Maltese Mushroom). Journal of Ethnopharmacology. 2019; 249 ():112368.
Chicago/Turabian StyleMarco Leonti; Sidonie Bellot; Paolo Zucca; Antonio Rescigno. 2019. "Astringent drugs for bleedings and diarrhoea: The history of Cynomorium coccineum (Maltese Mushroom)." Journal of Ethnopharmacology 249, no. : 112368.
Cynomorium coccineum is a non-photosynthetic plant that grows in Mediterranean countries and that is amply used in the traditional medicine. The aim of this study was to extend previous studies on the chemical and biological properties of C. coccineum, evaluating the potential antiviral and antiproliferative activity of the methanolic extract. The MTT assay was used for the in vitro cytotoxic studies against human cancer-derived cell lines, while both MTT and plaque reduction (PRT) methods were used to evaluate the potential inhibitory effect of the extract against a panel of mammal viruses. The results obtained showed no selective activity against any DNA and RNA virus but revealed an interesting antiproliferative activity against human leukaemia-derived cell lines.
Sarah Vascellari; Paolo Zucca; Daniela Perra; Alessandra Serra; Alessandra Piras; Antonio Rescigno. Antiproliferative and antiviral activity of methanolic extracts from Sardinian Maltese Mushroom (Cynomorium coccineum L.). Natural Product Research 2019, 1 -5.
AMA StyleSarah Vascellari, Paolo Zucca, Daniela Perra, Alessandra Serra, Alessandra Piras, Antonio Rescigno. Antiproliferative and antiviral activity of methanolic extracts from Sardinian Maltese Mushroom (Cynomorium coccineum L.). Natural Product Research. 2019; ():1-5.
Chicago/Turabian StyleSarah Vascellari; Paolo Zucca; Daniela Perra; Alessandra Serra; Alessandra Piras; Antonio Rescigno. 2019. "Antiproliferative and antiviral activity of methanolic extracts from Sardinian Maltese Mushroom (Cynomorium coccineum L.)." Natural Product Research , no. : 1-5.
In the continuous scientific search for new safe and effective drugs, there has recently been a rediscovery of natural substances as a potential reservoir of innovative therapeutic solutions for human health, with the prospect of integrating with and sometimes replacing conventional drugs. Cynomorium coccineum subsp. coccineum is a holoparasitic plant well known in ethnopharmacology, although its current use as a curative remedy is reported only in some ethnic groups of North Africa and the Arabian Peninsula. Often known as 'Maltese mushroom' due to its unique appearance and the absence of chlorophyll, C. coccineum is present in almost all of the Mediterranean Basin. It is only recently that a few research groups have begun to look for confirmation of some of its traditional uses to highlight previously unknown biological activities. Here, we review the recent scientific findings on the plant's phytochemistry and the most significant descriptions of some of its antioxidant and biological activities (antimicrobial, anticancer, pro-erectile, and anti-tyrosinase enzyme) both in vivo and in vitro. Some of these may be promising from the perspective of food and cosmetic formulations. The purpose of this review is to provide an initial impetus to those who, in the foreseeable future, will want to increase the knowledge and possible applications of this plant full of history, charm, and mystery.
Paolo Zucca; Sidonie Bellot; Antonio Rescigno. The Modern Use of an Ancient Plant: Exploring the Antioxidant and Nutraceutical Potential of the Maltese Mushroom (Cynomorium Coccineum L.). Antioxidants 2019, 8, 289 .
AMA StylePaolo Zucca, Sidonie Bellot, Antonio Rescigno. The Modern Use of an Ancient Plant: Exploring the Antioxidant and Nutraceutical Potential of the Maltese Mushroom (Cynomorium Coccineum L.). Antioxidants. 2019; 8 (8):289.
Chicago/Turabian StylePaolo Zucca; Sidonie Bellot; Antonio Rescigno. 2019. "The Modern Use of an Ancient Plant: Exploring the Antioxidant and Nutraceutical Potential of the Maltese Mushroom (Cynomorium Coccineum L.)." Antioxidants 8, no. 8: 289.
This work is an updated snapshot of Lamium plants and their biological activities. The main features of the plant are described and the components of its essential oils are summarized. The traditional medicinal uses of Lamium plants has been reported. The presence of these chemicals i.e., hydroxycinnamic acids, iridoids, secoiridoids, flavonoids, anthocyanins, phenylpropanoids, phytoecdysteroids, benzoxazinoids, betaine can provide biological activities. After the discussion of antioxidant properties documented for Lamium plants, the biological activities, studied using in vitro models, antimicrobial, antiviral, anti-inflammatory, anti-nociceptive activity, and pain therapy and cytotoxicity and cytoprotective activity are here described and discussed. Finally, targeted examples of in vivo studies are reported.
Bahare Salehi; Lorene Armstrong; Antonio Rescigno; Balakyz Yeskaliyeva; Gulnaz Seitimova; Ahmet Beyatli; Jugreet Sharmeen; Mohamad Fawzi Mahomoodally; Farukh Sharopov; Alessandra Durazzo; Massimo Lucarini; Antonello Santini; Ludovico Abenavoli; Raffaele Capasso; Javad Sharifi-Rad. Lamium Plants-A Comprehensive Review on Health Benefits and Biological Activities. Molecules 2019, 24, 1913 .
AMA StyleBahare Salehi, Lorene Armstrong, Antonio Rescigno, Balakyz Yeskaliyeva, Gulnaz Seitimova, Ahmet Beyatli, Jugreet Sharmeen, Mohamad Fawzi Mahomoodally, Farukh Sharopov, Alessandra Durazzo, Massimo Lucarini, Antonello Santini, Ludovico Abenavoli, Raffaele Capasso, Javad Sharifi-Rad. Lamium Plants-A Comprehensive Review on Health Benefits and Biological Activities. Molecules. 2019; 24 (10):1913.
Chicago/Turabian StyleBahare Salehi; Lorene Armstrong; Antonio Rescigno; Balakyz Yeskaliyeva; Gulnaz Seitimova; Ahmet Beyatli; Jugreet Sharmeen; Mohamad Fawzi Mahomoodally; Farukh Sharopov; Alessandra Durazzo; Massimo Lucarini; Antonello Santini; Ludovico Abenavoli; Raffaele Capasso; Javad Sharifi-Rad. 2019. "Lamium Plants-A Comprehensive Review on Health Benefits and Biological Activities." Molecules 24, no. 10: 1913.
Tyrosinase is one of the key enzymes in mammalian melanin biosynthesis. Decreasing tyrosinase activity has been targeted for the prevention of conditions related to the hyperpigmentation of the skin, such as melasma and age spots. This paper is devoted to the engineering of vesicle formulations loaded with 3-hydroxycoumarin for topical pharmaceutical applications. At first, it was demonstrated the strong inhibiting ability of 3-hydroxycoumarin against recombinant human tyrosinase. Then, such a drug was effectively encapsulated within liquid or gel-like vesicle formulations, both based on monoolein and lauroylcholine chloride. In vitro skin penetration and permeation studies proved these formulations efficiently overcome the barrier represented by the stratum corneum, delivering 3-hydroxycoumarin to the deeper skin layers. The effect of applying for different times the liquid and the gel formulation was also evaluated. Results revealed that application of the gel formulation for 2 hours favored the drug accumulation into the skin with low transdermal delivery, thus indicating this combination of administration time and formulation as ideal to locally inhibit tyrosinase activity with minimal systemic absorption. Moreover, when incubated with B16F10 melanoma cells, the liquid vesicle formulations did not show cytotoxic activity.
Michele Schlich; Marco Fornasier; Mariella Nieddu; Chiara Sinico; Sergio Murgia; Antonio Rescigno. 3-hydroxycoumarin loaded vesicles for recombinant human tyrosinase inhibition in topical applications. Colloids and Surfaces B: Biointerfaces 2018, 171, 675 -681.
AMA StyleMichele Schlich, Marco Fornasier, Mariella Nieddu, Chiara Sinico, Sergio Murgia, Antonio Rescigno. 3-hydroxycoumarin loaded vesicles for recombinant human tyrosinase inhibition in topical applications. Colloids and Surfaces B: Biointerfaces. 2018; 171 ():675-681.
Chicago/Turabian StyleMichele Schlich; Marco Fornasier; Mariella Nieddu; Chiara Sinico; Sergio Murgia; Antonio Rescigno. 2018. "3-hydroxycoumarin loaded vesicles for recombinant human tyrosinase inhibition in topical applications." Colloids and Surfaces B: Biointerfaces 171, no. : 675-681.
Cynomorium coccineum is a parasitic plant that has been known for centuries in ethnopharmacology. However, its biological activities have been scarcely studied, particularly in the case of plant grown in North Africa. Thus, we compared the chemical composition and antioxidant potential of C. coccineum taken from two regions characterized by very different climates: the Tataouine region in southeast Tunisia, which lies near the desert, and Sardinia in south Italy, which lies near the coast. The antioxidant potential of freeze-dried specimens from the hexane, ethyl acetate, acetone, methanolic, and aqueous extracts was tested using both electron transfer (ET) methods (i.e., TEAC-ABTS, FRAP, and DPPH) and hydrogen atom transfer (HAT) assay (ORAC-PYR). The metabolic profiles of both the methanolic and aqueous extracts were further investigated by 1H NMR spectroscopy. We also obtained fixed oils by supercritical fractioned extraction (SFE) with CO2. GC and HPLC were then used combined to determine the fatty acids profile. All extracts showed significantly different antioxidant capacities. 1H NMR spectroscopy showed evidence for the presence of amino acids, organic acids, and carbohydrates. The main fatty acids in the oils were 18:1 n-9, 18:2 n-6, 16:0, and 18:3 n-3. All the results showed a difference in chemical composition that influenced the total antioxidant power and the biological activity, verifying that climate affects the chemical composition of plants.
Imen Ben Attia; Paolo Zucca; Flaminia Cesare Marincola; Alessandra Piras; Antonella Rosa; Mohamed Chaieb; Antonio Rescigno. Chemical Composition and Antioxidant Potential Differences between Cynomorium coccineum L. Growing in Italy and in Tunisia: Effect of Environmental Stress. Diversity 2018, 10, 53 .
AMA StyleImen Ben Attia, Paolo Zucca, Flaminia Cesare Marincola, Alessandra Piras, Antonella Rosa, Mohamed Chaieb, Antonio Rescigno. Chemical Composition and Antioxidant Potential Differences between Cynomorium coccineum L. Growing in Italy and in Tunisia: Effect of Environmental Stress. Diversity. 2018; 10 (3):53.
Chicago/Turabian StyleImen Ben Attia; Paolo Zucca; Flaminia Cesare Marincola; Alessandra Piras; Antonella Rosa; Mohamed Chaieb; Antonio Rescigno. 2018. "Chemical Composition and Antioxidant Potential Differences between Cynomorium coccineum L. Growing in Italy and in Tunisia: Effect of Environmental Stress." Diversity 10, no. 3: 53.
The byssus of Pinna nobilis, the largest bivalve mollusc in the Mediterranean Sea, was investigated by histochemistry, immunohistochemistry, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). At low magnification, the byssus threads appeared distinctively elliptical in cross-section, with a typical size approaching 50 x 25 micron and a featureless glassy appearance. Histochemical and immunohistochemical techniques confirmed the presence of elastic domains but the absence of collagen, which is known to be the main component in other molluscs. Ultrastructural analysis by TEM revealed the presence of at least two components within the thread, and an inner arrangement of straight, tightly packed longitudinal streaks. SEM observations while confirming the inner packing of straight, parallel subfibrils, suggested in the fracture surfaces the presence of unidentified substance which cemented together the same subfibrils and which was removed by exposure to extreme pH values. AFM micrographs added further evidence for the tight packing of subfibrils and provided some evidence of orthogonal, barely visible connecting structures. Finally, HCl or NaOH treatment left the subfibrils clean and free from any other component.Â
Andrea Diana; Marcella Reguzzoni; Terenzio Congiu; Antonio Rescigno; Federica Sollai; Mario Raspanti. The byssus threads of Pinna nobilis: A histochemical and ultrastructural study. European Journal of Histochemistry 2017, 61, 1 .
AMA StyleAndrea Diana, Marcella Reguzzoni, Terenzio Congiu, Antonio Rescigno, Federica Sollai, Mario Raspanti. The byssus threads of Pinna nobilis: A histochemical and ultrastructural study. European Journal of Histochemistry. 2017; 61 (4):1.
Chicago/Turabian StyleAndrea Diana; Marcella Reguzzoni; Terenzio Congiu; Antonio Rescigno; Federica Sollai; Mario Raspanti. 2017. "The byssus threads of Pinna nobilis: A histochemical and ultrastructural study." European Journal of Histochemistry 61, no. 4: 1.
Cynomorium coccineum L., the desert thumb, is a rather exotic, parasitic plant unable to engage in photosynthesis, yet rich in a variety of unique compounds with a wide spectrum of biological applications. Whilst extraction, separation and isolation of such compounds is time consuming, the particular properties of the plant, such as dryness, hardness and lack of chlorophyll, render it a prime target for possible nanosizing. The entire plant, the external layer (coat) as well as its peel, are readily milled and high pressure homogenized to yield small, mostly uniform spherical particles with diameters in the range of 300 to 600 nm. The best quality of particles is obtained for the processed entire plant. Based on initial screens for biological activity, it seems that these particles are particularly active against the pathogenic fungus Candida albicans, whilst no activity could be observed against the model nematode Steinernema feltiae. This activity is particularly pronounced in the case of the external layer, whilst the peeled part does not seem to inhibit growth of C. albicans. Thanks to the ease of sample preparation, the good quality of the nanosuspension obtained, and the interesting activity of this natural product, nanosized coats of Cynomorium may well provide a lead for future development and applications as “green” materials in the field of medicine, but also environmentally, for instance in agriculture.
Sharoon Griffin; Reem Alkhayer; Seda Mirzoyan; Astghik Turabyan; Paolo Zucca; Muhammad Sarfraz; Muhammad Jawad Nasim; Armen Trchounian; Antonio Rescigno; Cornelia M. Keck; Claus Jacob. Nanosizing Cynomorium: Thumbs up for Potential Antifungal Applications. Inventions 2017, 2, 24 .
AMA StyleSharoon Griffin, Reem Alkhayer, Seda Mirzoyan, Astghik Turabyan, Paolo Zucca, Muhammad Sarfraz, Muhammad Jawad Nasim, Armen Trchounian, Antonio Rescigno, Cornelia M. Keck, Claus Jacob. Nanosizing Cynomorium: Thumbs up for Potential Antifungal Applications. Inventions. 2017; 2 (3):24.
Chicago/Turabian StyleSharoon Griffin; Reem Alkhayer; Seda Mirzoyan; Astghik Turabyan; Paolo Zucca; Muhammad Sarfraz; Muhammad Jawad Nasim; Armen Trchounian; Antonio Rescigno; Cornelia M. Keck; Claus Jacob. 2017. "Nanosizing Cynomorium: Thumbs up for Potential Antifungal Applications." Inventions 2, no. 3: 24.
Maltese Mushroom (Cynomorium coccineum L.) is a non-photosynthetic plant that has been used in traditional medicine for many centuries. In this paper, water extracts from the whole plant, external layer and peeled plant were studied to determine the main components responsible for its biological activities, i.e., its antimicrobial, antioxidant, and anti-tyrosinase activities; its cytotoxicity against mouse melanoma B16F10 cells; and its pro-erectile activity in adult male rats. The results of electron transfer and hydrogen transfer assays showed that the antioxidant activity was mainly due to anthocyanins in the external layer, whereas the external layer and peeled plant extracts both inhibited the microbial growth of several Gram-positive strains. In contrast, the whole plant extract had the highest anti-tyrosinase activity and exhibited pro-erectile activity when administered subcutaneously. Overall, this study elucidated which parts of Maltese Mushroom are responsible for its antimicrobial, antioxidant, and anti-tyrosinase activities and thus which extracts have potential for use in nutraceutical formulations.
Paolo Zucca; Antonio Argiolas; Mariella Nieddu; Manuela Pintus; Antonella Rosa; Fabrizio Sanna; Francesca Sollai; Daniela Steri; Antonio Rescigno. Biological Activities and Nutraceutical Potentials of Water Extracts from Different Parts of Cynomorium coccineum L. (Maltese Mushroom). Polish Journal of Food and Nutrition Sciences 2016, 66, 179 -188.
AMA StylePaolo Zucca, Antonio Argiolas, Mariella Nieddu, Manuela Pintus, Antonella Rosa, Fabrizio Sanna, Francesca Sollai, Daniela Steri, Antonio Rescigno. Biological Activities and Nutraceutical Potentials of Water Extracts from Different Parts of Cynomorium coccineum L. (Maltese Mushroom). Polish Journal of Food and Nutrition Sciences. 2016; 66 (3):179-188.
Chicago/Turabian StylePaolo Zucca; Antonio Argiolas; Mariella Nieddu; Manuela Pintus; Antonella Rosa; Fabrizio Sanna; Francesca Sollai; Daniela Steri; Antonio Rescigno. 2016. "Biological Activities and Nutraceutical Potentials of Water Extracts from Different Parts of Cynomorium coccineum L. (Maltese Mushroom)." Polish Journal of Food and Nutrition Sciences 66, no. 3: 179-188.
Manel Belkhir; Karima Dhaouadi; Antonella Rosa; Angela Atzeri; Mariella Nieddu; Carlo Tuberoso; Antonio Rescigno; Mohamed Amri; Sami Fattouch. Protective effects of azarole polyphenolic extracts against oxidative damage using in vitro biomolecular and cellular models. Industrial Crops and Products 2016, 86, 239 -250.
AMA StyleManel Belkhir, Karima Dhaouadi, Antonella Rosa, Angela Atzeri, Mariella Nieddu, Carlo Tuberoso, Antonio Rescigno, Mohamed Amri, Sami Fattouch. Protective effects of azarole polyphenolic extracts against oxidative damage using in vitro biomolecular and cellular models. Industrial Crops and Products. 2016; 86 ():239-250.
Chicago/Turabian StyleManel Belkhir; Karima Dhaouadi; Antonella Rosa; Angela Atzeri; Mariella Nieddu; Carlo Tuberoso; Antonio Rescigno; Mohamed Amri; Sami Fattouch. 2016. "Protective effects of azarole polyphenolic extracts against oxidative damage using in vitro biomolecular and cellular models." Industrial Crops and Products 86, no. : 239-250.
R. Orrù; Paolo Zucca; M. Falzoi; E. Atzori; Antonio Rescigno; A. Padiglia. First step towards the biomolecular characterization of Pompia, an endemic Citrus-like fruit from Sardinia (Italy). Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 2016, 151, 464 -473.
AMA StyleR. Orrù, Paolo Zucca, M. Falzoi, E. Atzori, Antonio Rescigno, A. Padiglia. First step towards the biomolecular characterization of Pompia, an endemic Citrus-like fruit from Sardinia (Italy). Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 2016; 151 (3):464-473.
Chicago/Turabian StyleR. Orrù; Paolo Zucca; M. Falzoi; E. Atzori; Antonio Rescigno; A. Padiglia. 2016. "First step towards the biomolecular characterization of Pompia, an endemic Citrus-like fruit from Sardinia (Italy)." Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology 151, no. 3: 464-473.
Kojic acid is a natural antifungal and antibacterial agent that has been extensively studied for its tyrosinase inhibitory and metal coordination properties. Tyrosinase is a metalloenzyme with two copper ions in the active site. It is widely accepted that the tyrosinase inhibitory activity of kojic acid is related to its ability to coordinate metals. Over the past five years, we have used kojic acid to synthesize new and efficient bis-kojic acid chelators of iron and aluminium. In parallel, we investigated whether the de novo designed ligands could interfere with proper tyrosinase functioning. The present study combines our experience with inhibition and coordination studies of the new ligand: Kojic-βAla-Kojic. Research aimed at the assembly of a new potent tyrosinase inhibitor was based on the well-known crystal structure of the enzyme. Two questions were whether two kojic acids could act better than one and to what extent the length and kind of linker could ameliorate metal coordination, and inhibitory activity. Our results show that Kojic-βAla-Kojic has high affinity for Fe(III), Al(III), Zn(II), and Cu(II) and strong tyrosinase inhibitory effect and it can be proposed for use in industrial and pharmaceutical applications.
Joanna Izabela Lachowicz; Valeria Marina Nurchi; Guido Crisponi; María De Guadalupe Jaraquemada-Peláez; Antonio Rescigno; Piotr Stefanowicz; Marta Cal; Zbigniew Szewczuk. Metal coordination and tyrosinase inhibition studies with Kojic-βAla-Kojic. Journal of Inorganic Biochemistry 2015, 151, 36 -43.
AMA StyleJoanna Izabela Lachowicz, Valeria Marina Nurchi, Guido Crisponi, María De Guadalupe Jaraquemada-Peláez, Antonio Rescigno, Piotr Stefanowicz, Marta Cal, Zbigniew Szewczuk. Metal coordination and tyrosinase inhibition studies with Kojic-βAla-Kojic. Journal of Inorganic Biochemistry. 2015; 151 ():36-43.
Chicago/Turabian StyleJoanna Izabela Lachowicz; Valeria Marina Nurchi; Guido Crisponi; María De Guadalupe Jaraquemada-Peláez; Antonio Rescigno; Piotr Stefanowicz; Marta Cal; Zbigniew Szewczuk. 2015. "Metal coordination and tyrosinase inhibition studies with Kojic-βAla-Kojic." Journal of Inorganic Biochemistry 151, no. : 36-43.
The structure–activity relationships of four hydroxycoumarins, two with the hydroxyl group on the aromatic ring of the molecule and two with the hydroxyl group replacing hydrogen of the pyrone ring, and their interactions with mushroom tyrosinase were studied. These compounds displayed different behaviors upon action of the enzyme. The two compounds, ar-hydroxylated 6-hydroxycoumarin and 7-hydroxycoumarin, were both weak substrates of the enzyme. Interestingly, in both cases, the product of the catalysis was the 6,7-hydroxycoumarin, although 5,6- and 7,8-isomers could also theoretically be formed. Additionally, both were able to reduce the formation of dopachrome when tyrosinase acted on its typical substrate, l-tyrosine. Although none of the compounds that contained a hydroxyl group on the pyrone ring were substrates of tyrosinase, the 3-hydroxycoumarin was a potent inhibitor of the enzyme, and the 4-hydroxycoumarin was not an inhibitor. These results were compared with those obtained by in silico molecular docking predictions to obtain potentially useful information for the synthesis of new coumarin-based inhibitors that resemble the structure of the 3-hydroxycoumarin.
Shailendra Asthana; Paolo Zucca; Attilio Vargiu; Enrico Sanjust; Paolo Ruggerone; Antonio Rescigno. Structure–Activity Relationship Study of Hydroxycoumarins and Mushroom Tyrosinase. Journal of Agricultural and Food Chemistry 2015, 63, 7236 -7244.
AMA StyleShailendra Asthana, Paolo Zucca, Attilio Vargiu, Enrico Sanjust, Paolo Ruggerone, Antonio Rescigno. Structure–Activity Relationship Study of Hydroxycoumarins and Mushroom Tyrosinase. Journal of Agricultural and Food Chemistry. 2015; 63 (32):7236-7244.
Chicago/Turabian StyleShailendra Asthana; Paolo Zucca; Attilio Vargiu; Enrico Sanjust; Paolo Ruggerone; Antonio Rescigno. 2015. "Structure–Activity Relationship Study of Hydroxycoumarins and Mushroom Tyrosinase." Journal of Agricultural and Food Chemistry 63, no. 32: 7236-7244.
The present study aimed to examine the potential anticancer properties of fixed oil obtained from Maltese mushroom (Cynomorium coccineum L.), an edible, non-photosynthetic plant, used in traditional medicine of Mediterranean countries to treat various ailments and as an emergency food during the famine. We investigated the effect of the oil, obtained from dried stems by supercritical fractioned extraction with CO2, on B16F10 melanoma and colon cancer Caco-2 cell viability and lipid profile. The oil, rich in essential fatty acids (18:3n-3 and 18:2n-6), showed a significant growth inhibitory effect on melanoma and colon cancer cells. The incubation (24 h) with non-toxic oil concentrations (25 and 50 μg/mL) induced in both cancer cell lines a significant accumulation of the fatty acids 18:3n-3 and 18:2n-6 and an increase of the cellular levels of eicosapentaenoic acid (20:5n-3) with anticancer activity. Moreover, the oil exhibited the ability to potentiate the growth inhibitory effect of the antitumor drug 5-fluorouracil in Caco-2 cells and to influence the melanin content in B16F10 cells. The results qualify C. coccineum as a resource of oil, with potential benefits in cancer prevention, for nutraceutical and pharmaceutical applications.
Antonella Rosa; Mariella Nieddu; Alessandra Piras; Angela Atzeri; Danilo Putzu; Antonio Rescigno. Maltese Mushroom (Cynomorium coccineum L.) as Source of Oil with Potential Anticancer Activity. Nutrients 2015, 7, 849 -864.
AMA StyleAntonella Rosa, Mariella Nieddu, Alessandra Piras, Angela Atzeri, Danilo Putzu, Antonio Rescigno. Maltese Mushroom (Cynomorium coccineum L.) as Source of Oil with Potential Anticancer Activity. Nutrients. 2015; 7 (2):849-864.
Chicago/Turabian StyleAntonella Rosa; Mariella Nieddu; Alessandra Piras; Angela Atzeri; Danilo Putzu; Antonio Rescigno. 2015. "Maltese Mushroom (Cynomorium coccineum L.) as Source of Oil with Potential Anticancer Activity." Nutrients 7, no. 2: 849-864.