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Human adenovirus (HAdV) is recognized as frequent cause of acute gastroenteritis and enteric viruses can be preserved in frozen stored feces for long periods of times. The purpose of the present study was to investigate enteric HAdV genotypic diversity in archival fecal specimens stored from 1998 to 2005 in order to understand the natural history of HAdV in diarrheal patients in Brazil before rotavirus vaccine introduction. A total of 3346 specimens were tested for HAdV using conventional PCR. Genotypes were identified by sequencing. HAdV was detected in 6.8% (228/3346). Positivity was higher in children ≤ 5 years and males (p < 0.05). HAdV was most frequently observed during winter and spring seasons (p < 0.05). HAdV-F41 was the most prevalent genotype (59.2%;135/228), followed by HAdV-F40 (16.2%;37/228), HAdV-C1 (5.2%;12/228), HAdV-C2 (5.2%;12/228), HAdV-C5 (3.1%;7/228), HAdV-A12 (1.3%;3/228), HAdV-E4 (0.9%;2/228), HAdV-B3 (0.9%;2/228) and HAdV-B21 (0.4%;1/228). In 7.6% (17/228) only species D could be defined. HAdV-E4 strains were phylogenetic analyzed and classified as lineage (a)-like PG II. HAdV prevalence remained stable in Brazilian population, regardless rotavirus vaccine introduction. The predominant HAdV genotypes detected did not change over time, highlighting a high diversity of circulating strains in the country throughout decades. Due to the historical lack of HAdV genotyping surveillance in Brazil, HAdV-E4 epidemiology is virtually unknown in the country. The present study contributed significantly to the understanding of the natural history of HAdV in diarrheal patients in Brazil. The acquired data are important for clinical diagnosis, particularly for studies investigating enteric viruses' prevalence and molecular epidemiology of archival clinical specimens.
Yasmin França Viana Pires de Souza; Ellen Viana de Souza; Lais Sampaio de Azevedo; Roberta Salzone Medeiros; Maria Do Carmo Sampaio Tavares Timenetsky; Adriana Luchs. Enteric adenovirus epidemiology from historical fecal samples in Brazil (1998–2005): Pre-rotavirus vaccine era. Infection, Genetics and Evolution 2021, 94, 105007 .
AMA StyleYasmin França Viana Pires de Souza, Ellen Viana de Souza, Lais Sampaio de Azevedo, Roberta Salzone Medeiros, Maria Do Carmo Sampaio Tavares Timenetsky, Adriana Luchs. Enteric adenovirus epidemiology from historical fecal samples in Brazil (1998–2005): Pre-rotavirus vaccine era. Infection, Genetics and Evolution. 2021; 94 ():105007.
Chicago/Turabian StyleYasmin França Viana Pires de Souza; Ellen Viana de Souza; Lais Sampaio de Azevedo; Roberta Salzone Medeiros; Maria Do Carmo Sampaio Tavares Timenetsky; Adriana Luchs. 2021. "Enteric adenovirus epidemiology from historical fecal samples in Brazil (1998–2005): Pre-rotavirus vaccine era." Infection, Genetics and Evolution 94, no. : 105007.
Investigation of human enterovirus (EV) in diarrheic fecal specimens is valuable to address EV diversity circulating worldwide. However, the detection of EV strains exclusively in fecal specimens must be interpreted cautiously. EV are well known causative agents associated with a spectrum of human diseases, but not acute gastroenteritis. EV isolation in stool samples could not necessarily be associated with diarrheic symptoms, as most EV infections appear to be asymptomatic, and healthy children could excrete EV in their stool. The diagnostic of EV is only confirmed when the neutralization test presents a significant increase in antibody titers (three times or more) in the paired serum samples (acute-phase and convalescent-phase) against the same EV serotype isolated in feces. In addition, patients suffering from acute gastroenteritis, even during an EV investigation, must be screened in parallel for gastroenteric viruses (i.e. norovirus and rotavirus) in order to clarify if the symptoms could be linked to other viral agent detected in their fecal samples. Surveillance of EV diversity among distinct patient groups, including diarrheic individuals, must be taken into consideration and can considerably increase the power of non-polio EV surveillance system in Brazil. More well-designed studies are necessary to further elucidate the role of EV in acute gastroenteritis.
Adriana Luchs. Comments on “Detection and identification of enteroviruses circulating in children with acute gastroenteritis in Pará State, Northern Brazil (2010–2011)”. Virology Journal 2021, 18, 1 -3.
AMA StyleAdriana Luchs. Comments on “Detection and identification of enteroviruses circulating in children with acute gastroenteritis in Pará State, Northern Brazil (2010–2011)”. Virology Journal. 2021; 18 (1):1-3.
Chicago/Turabian StyleAdriana Luchs. 2021. "Comments on “Detection and identification of enteroviruses circulating in children with acute gastroenteritis in Pará State, Northern Brazil (2010–2011)”." Virology Journal 18, no. 1: 1-3.
Echoviruses (E) are a diverse group of viruses responsible for various pathological conditions in humans including aseptic meningitis, myocarditis, and acute flaccid paralysis. The detection and identification of echovirus genotypes in clinical samples is challenging due to its high genetic diversity. Here, we report the complete genome sequences of nine echoviruses, obtained by next-generation sequencing of 238 fecal samples from individuals with gastroenteritis in regions of Brazil. Detected viruses were classified into six genotypes: Three E1 sequences (BRA/TO-028, BRA/TO-069 and BRA/TO-236), one E3 (BRA/TO-018), one E11 (BRA/TO-086), one E20 (BRA/TO-016), two E29 (BRA/TO-030 and BRA/TO-193), and one E30 sequence (BRA/TO-032). Phylogenetic analysis indicated that the echoviruses E1 and E29 circulating in Brazil are divergent from strains circulating worldwide. The genotype diversity identified in our study may under-represent the total echovirus diversity in Brazil because of the small sample size and the restricted geographical distribution covered by the survey.
Endrya Do Socorro Fôro Ramos; Ulisses Rosa; Geovani De Oliveira Ribeiro; Fabiola Villanova; Flávio de Pádua Milagres; Rafael Brustulin; Vanessa Dos Santos Morais; Mayara Bertanhe; Roberta Marcatti; Emerson Araújo; Steven Witkin; Eric Delwart; Adriana Luchs; Antonio da Costa; Élcio Leal. High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis. Viruses 2021, 13, 595 .
AMA StyleEndrya Do Socorro Fôro Ramos, Ulisses Rosa, Geovani De Oliveira Ribeiro, Fabiola Villanova, Flávio de Pádua Milagres, Rafael Brustulin, Vanessa Dos Santos Morais, Mayara Bertanhe, Roberta Marcatti, Emerson Araújo, Steven Witkin, Eric Delwart, Adriana Luchs, Antonio da Costa, Élcio Leal. High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis. Viruses. 2021; 13 (4):595.
Chicago/Turabian StyleEndrya Do Socorro Fôro Ramos; Ulisses Rosa; Geovani De Oliveira Ribeiro; Fabiola Villanova; Flávio de Pádua Milagres; Rafael Brustulin; Vanessa Dos Santos Morais; Mayara Bertanhe; Roberta Marcatti; Emerson Araújo; Steven Witkin; Eric Delwart; Adriana Luchs; Antonio da Costa; Élcio Leal. 2021. "High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis." Viruses 13, no. 4: 595.
From 2010-2016, a total of 251 stool samples were screened for norovirus using next-generation sequencing (NGS) followed by phylogenetic analysis to investigate the genotypic diversity of noroviruses in rural and low-income urban areas in northern Brazil. Norovirus infection was detected in 19.9% (50/251) of the samples. Eight different genotypes were identified: GII.4_Sydney[P31] (64%, 32/50), GII.6[P7] (14%, 7/50), GII.17[P17] (6%, 3/50), GII.1[P33] (6%, 3/50), GII.3[P16] (4%, 2/50), GII.2[P16] (2%, 1/50), GII.2[P2] (2%, 1/50), and GII.4_New Orleans[P4] (2%, 1/50). Distinct GII.6[P7] variants were recognized, indicating the presence of different co-circulating strains. Elucidating norovirus genetic diversity will improve our understanding of their potential health burden, in particular for the GII.4_Sydney[P31] variant.
Rory J. Tinker; Antonio Charlys da Costa; Roozbeh Tahmasebi; Flavio Augusto De Pádua Milagres; Vanessa Dos Santos Morais; Ramendra Pati Pandey; Alexis José-Abrego; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Mariana Sequetin Cunha; Emerson Luiz Lima Araújo; Mariela Martínez Gómez; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Elcio Leal; Adriana Luchs. Norovirus strains in patients with acute gastroenteritis in rural and low-income urban areas in northern Brazil. Archives of Virology 2021, 166, 905 -913.
AMA StyleRory J. Tinker, Antonio Charlys da Costa, Roozbeh Tahmasebi, Flavio Augusto De Pádua Milagres, Vanessa Dos Santos Morais, Ramendra Pati Pandey, Alexis José-Abrego, Rafael Brustulin, Maria Da Aparecida Rodrigues Teles, Mariana Sequetin Cunha, Emerson Luiz Lima Araújo, Mariela Martínez Gómez, Xutao Deng, Eric Delwart, Ester Cerdeira Sabino, Elcio Leal, Adriana Luchs. Norovirus strains in patients with acute gastroenteritis in rural and low-income urban areas in northern Brazil. Archives of Virology. 2021; 166 (3):905-913.
Chicago/Turabian StyleRory J. Tinker; Antonio Charlys da Costa; Roozbeh Tahmasebi; Flavio Augusto De Pádua Milagres; Vanessa Dos Santos Morais; Ramendra Pati Pandey; Alexis José-Abrego; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Mariana Sequetin Cunha; Emerson Luiz Lima Araújo; Mariela Martínez Gómez; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Elcio Leal; Adriana Luchs. 2021. "Norovirus strains in patients with acute gastroenteritis in rural and low-income urban areas in northern Brazil." Archives of Virology 166, no. 3: 905-913.
During 2006-2011, 5035 fecal samples were tested by PCR for human adenovirus (HAdV) and sequenced. HAdV was detected in 198 cases (3.9%), with the highest rate in children ≤ 5 years. Enteric HAdVs were the most prevalent genotypes (78%; 146/187): HAdV-F41 (63.6%; 119/187), HAdV-F40 (12.3%; 23/187), HAdV-A12 (1.6%; 3/187) and HAdV-A31 (0.5%; 1/187). Non-enteric HAdVs were detected in 22% (41/187): HAdV-C1 (8.0%; 15/187), HAdV-C2 (6.9%; 13/187), HAdV-C5 (4.3%; 8/187), HAdV-D8 (1.3%; 2/187), HAdV-B21 (0.5%; 1/187), HAdV-B3 (0.5%; 1/187) and HAdV-C6 (0.5%; 1/187). This 6-year retrospective study points out a high diversity of HAdV types circulating in Brazil and highlights the need to carry out molecular epidemiological studies of HAdV among patients with acute diarrheal infection on a regular basis.
Ellen Viana de Souza; Yasmin França Viana Pires de Souza; Roberta Salzone Medeiros; Lais Sampaio de Azevedo; Talita Gonçalves Aires de Queiroz; Rodrigo Lopes Sanz-Duro; Robson Dos Santos Souza Marinho; Shirley Vasconcelos Komninakis; Maria Do Carmo Sampaio Tavares Timenetsky; Adriana Luchs. Diversity of enteric and non-enteric human adenovirus strains in Brazil, 2006-2011. Archives of Virology 2021, 166, 897 -903.
AMA StyleEllen Viana de Souza, Yasmin França Viana Pires de Souza, Roberta Salzone Medeiros, Lais Sampaio de Azevedo, Talita Gonçalves Aires de Queiroz, Rodrigo Lopes Sanz-Duro, Robson Dos Santos Souza Marinho, Shirley Vasconcelos Komninakis, Maria Do Carmo Sampaio Tavares Timenetsky, Adriana Luchs. Diversity of enteric and non-enteric human adenovirus strains in Brazil, 2006-2011. Archives of Virology. 2021; 166 (3):897-903.
Chicago/Turabian StyleEllen Viana de Souza; Yasmin França Viana Pires de Souza; Roberta Salzone Medeiros; Lais Sampaio de Azevedo; Talita Gonçalves Aires de Queiroz; Rodrigo Lopes Sanz-Duro; Robson Dos Santos Souza Marinho; Shirley Vasconcelos Komninakis; Maria Do Carmo Sampaio Tavares Timenetsky; Adriana Luchs. 2021. "Diversity of enteric and non-enteric human adenovirus strains in Brazil, 2006-2011." Archives of Virology 166, no. 3: 897-903.
Enterovirus (EV) is commonly associated with central nervous system (CNS) syndromes. Recently, gastroenteric viruses, including rotavirus (RVA), human astrovirus (HAstV), and norovirus (NoV), have also been associated with CNS neurological disorders. The aim of the present study was to investigate the presence of EV, RVA, HAst, and NoV associated to CNS infections with undiagnosed etiology in Northwest region of São Paulo State, Brazil, and to conduct the molecular characterization of the positive samples detected. A total of 288 cerebrospinal fluid samples collected from July to December 2017 were tested for EV and NoV by quantitative real‐time polymerase chain reaction (RT‐qPCR), HAstV by conventional RT‐PCR, and RVA by enzyme‐linked immunosorbent assay. Positive‐EV samples were inoculated in cells lines, amplified by RT‐PCR and sequenced. RVA, NoV, and HAstV were not detected. EV infection was detected in 5.5% (16/288), and five samples successful genotyped: echovirus 3 (E3) (1/5), coxsackie virus A6 (CVA6) (1/5), and coxsackie virus B4 (CVB4) (3/5). Meningitis was the main syndrome observed (12/16; 75%). CVA6, CVB4, and E3 were identified associated with aseptic meningitis. Reports of CVA6 associated with aseptic meningitis are rare, E3 had not been previously reported in Brazil, and epidemiological data on CVB4 in the country is virtually unknown. The present investigation illustrates the circulation of diverse EV types in a small regional sample set and in a short period of time, highlighting the importance of an active EV surveillance system in CNS infections. Enhanced understanding of undiagnosed CNS infections will assist in public health and health care planning.
Leonardo Cecílio da Rocha; Cássia Fernanda Estofolete; Bruno Henrique Gonçalves De Aguiar Milhim; Marcos Tayar Augusto; Nathalia Zini; Gislaine Celestino Dutra da Silva; Hélio Correa Ferraz‐Junior; Vania Ms Brienze; Elisabete Liso; Mariana Sequetin Cunha; Ester Cerdeira Sabino; Antonio Charlys da Costa; Maurício Lacerda Nogueira; Adriana Luchs; Ana Carolina Bernardes Terzian. Enteric viruses circulating in undiagnosed central nervous system infections at tertiary hospital in São José do Rio Preto, São Paulo, Brazil. Journal of Medical Virology 2020, 93, 3539 -3548.
AMA StyleLeonardo Cecílio da Rocha, Cássia Fernanda Estofolete, Bruno Henrique Gonçalves De Aguiar Milhim, Marcos Tayar Augusto, Nathalia Zini, Gislaine Celestino Dutra da Silva, Hélio Correa Ferraz‐Junior, Vania Ms Brienze, Elisabete Liso, Mariana Sequetin Cunha, Ester Cerdeira Sabino, Antonio Charlys da Costa, Maurício Lacerda Nogueira, Adriana Luchs, Ana Carolina Bernardes Terzian. Enteric viruses circulating in undiagnosed central nervous system infections at tertiary hospital in São José do Rio Preto, São Paulo, Brazil. Journal of Medical Virology. 2020; 93 (6):3539-3548.
Chicago/Turabian StyleLeonardo Cecílio da Rocha; Cássia Fernanda Estofolete; Bruno Henrique Gonçalves De Aguiar Milhim; Marcos Tayar Augusto; Nathalia Zini; Gislaine Celestino Dutra da Silva; Hélio Correa Ferraz‐Junior; Vania Ms Brienze; Elisabete Liso; Mariana Sequetin Cunha; Ester Cerdeira Sabino; Antonio Charlys da Costa; Maurício Lacerda Nogueira; Adriana Luchs; Ana Carolina Bernardes Terzian. 2020. "Enteric viruses circulating in undiagnosed central nervous system infections at tertiary hospital in São José do Rio Preto, São Paulo, Brazil." Journal of Medical Virology 93, no. 6: 3539-3548.
Human Adenovirus species C (HAdV-C) is the most common etiologic agent of respiratory disease. In the present study, we characterized the nearly full-length genome of one potential new HAdV-C recombinant strain constituted by Penton and Fiber proteins belonging to type 89 and a chimeric Hexon protein of types 1 and 89. By using viral metagenomics techniques, we screened out, in the states of Tocantins and Pará, Northern and North regions of Brazil, from 2010 to 2016, 251 fecal samples of children between 0.5 to 2.5 years old. These children were presenting acute diarrhea not associated with common pathogens (i.e., rotavirus, norovirus). We identified two HAdV-C strains in two distinct patients. Phylogenetic analysis performed using all complete genomes available at GenBank database indicated that one strain (HAdV-C BR-245) belonged to type 1. The phylogenetic analysis also indicated that the second strain (HAdV-C BR-211) was located at the base of the clade formed by the newly HAdV-C strains type 89. Recombination analysis revealed that strain HAdV-C BR-211 is a chimera in which the variable regions of Hexon gene combined HAdV-C1 and HAdV-C89 sequences. Therefore, HAdV-C BR-211 strain possesses a genomic backbone of type HAdV-C89 and a unique insertion of HAdV-C1 in the Hexon sequence. Recombination may play an important driving force in HAdV-C diversity and evolution. Studies employing complete genomic sequencing on circulating HAdV-C strains in Brazil are needed to understand the clinical significance of the presented data.
Roozbeh Tahmasebi; Antonio Charlys Da Costa; Kaelan Tardy; Rory J. Tinker; Flavio Augusto De Padua Milagres; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Rogério Togisaki Das Chagas; Cassia Vitória De Deus Alves Soares; Aripuana Sakurada Aranha Watanabe; Cecilia Salete Alencar; Fabiola Villanova; Xutao Deng; Eric Delwart; Adriana Luchs; Élcio Leal; Ester Cerdeira Sabino. Genomic Analyses of Potential Novel Recombinant Human Adenovirus C in Brazil. Viruses 2020, 12, 508 .
AMA StyleRoozbeh Tahmasebi, Antonio Charlys Da Costa, Kaelan Tardy, Rory J. Tinker, Flavio Augusto De Padua Milagres, Rafael Brustulin, Maria Da Aparecida Rodrigues Teles, Rogério Togisaki Das Chagas, Cassia Vitória De Deus Alves Soares, Aripuana Sakurada Aranha Watanabe, Cecilia Salete Alencar, Fabiola Villanova, Xutao Deng, Eric Delwart, Adriana Luchs, Élcio Leal, Ester Cerdeira Sabino. Genomic Analyses of Potential Novel Recombinant Human Adenovirus C in Brazil. Viruses. 2020; 12 (5):508.
Chicago/Turabian StyleRoozbeh Tahmasebi; Antonio Charlys Da Costa; Kaelan Tardy; Rory J. Tinker; Flavio Augusto De Padua Milagres; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Rogério Togisaki Das Chagas; Cassia Vitória De Deus Alves Soares; Aripuana Sakurada Aranha Watanabe; Cecilia Salete Alencar; Fabiola Villanova; Xutao Deng; Eric Delwart; Adriana Luchs; Élcio Leal; Ester Cerdeira Sabino. 2020. "Genomic Analyses of Potential Novel Recombinant Human Adenovirus C in Brazil." Viruses 12, no. 5: 508.
Surveillance of Rotavirus A (RVA) throughout the national territory is important to establish a more complete epidemiological-molecular scenario of this virus circulation in Brazil. The aim of the present study was to investigate the genetic diversity of RVA strains circulating in Tocantins State (Northern Brazil) during six years of post-vaccination follow-up (2010-2016). A total of 248 stool samples were screened by next generation sequencing and 107 (43.1%) nearly full length RVA genome sequences were obtained; one sample was co-infected with two RVA strains (G2/G8P[4]). Six G and P genotypes combinations were detected: G12P[8] strains (78.6%), as well as the G3P[8] (9.3%) and G1P[8] (0.9%) were associated with a Wa-like genogroup backbone. All G2P[4] (5.6%) and G8P[4] (2.8%) strains, including the mixed G2/G8P[4] infection (0.9%) showed the DS-1-like genetic background. The two G12P[4] strains (1.9%) were associated with distinct genetic backbones: Wa-like and DS-1-like. The phylogenetic analysis revealed the circulation of lineages G1-I, G2-IV, G3-III, G8-I and G12-III, and P[4]-V and P[8]-III of the VP7 and VP4 genes, respectively. Conserved clustering pattern and low genetic diversity were observed regarding VP1-VP3 and VP6, as well as NSP1-5 segments. We identified the same RVA circulation pattern reported in other Brazilian regions in the period of 2010-2016, suggesting that rural and low-income areas may not have a different RVA genotypic distribution compared to other parts of the country. The unique presentation of whole-genome data of RVA strains detected in the Tocantins State provides a baseline for monitoring variations in the genetic composition of RVA in this area.
Marcelle Silva-Sales; Elcio Leal; Flavio Augusto De Pádua Milagres; Rafael Brustulin; Vanessa Dos Santos Morais; Roberta Marcatti; Emerson Luiz Lima Araújo; Steven S. Witkin; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Adriana Luchs; Antonio Charlys Da Costa. Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016). Revista do Instituto de Medicina Tropical de São Paulo 2020, 62, e98 .
AMA StyleMarcelle Silva-Sales, Elcio Leal, Flavio Augusto De Pádua Milagres, Rafael Brustulin, Vanessa Dos Santos Morais, Roberta Marcatti, Emerson Luiz Lima Araújo, Steven S. Witkin, Xutao Deng, Ester Cerdeira Sabino, Eric Delwart, Adriana Luchs, Antonio Charlys Da Costa. Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016). Revista do Instituto de Medicina Tropical de São Paulo. 2020; 62 ():e98.
Chicago/Turabian StyleMarcelle Silva-Sales; Elcio Leal; Flavio Augusto De Pádua Milagres; Rafael Brustulin; Vanessa Dos Santos Morais; Roberta Marcatti; Emerson Luiz Lima Araújo; Steven S. Witkin; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Adriana Luchs; Antonio Charlys Da Costa. 2020. "Genomic constellation of human Rotavirus A strains identified in Northern Brazil: a 6-year follow-up (2010-2016)." Revista do Instituto de Medicina Tropical de São Paulo 62, no. : e98.
We characterized the 24 nearly full-length genomes of human parechoviruses (PeV) from children in the north of Brazil. The initial phylogenetic analysis indicated that 17 strains belonged to genotype 1, 5 to genotype 4, and 1 to genotype 17. A more detailed analysis revealed a high frequency of recombinant strains (58%): A total of 14 of our PeV-As were chimeric, with four distinct recombination patterns identified. Five strains were composed of genotypes 1 and 5 (Rec1/5); five strains shared a complex mosaic pattern formed by genotypes 4, 5, and 17 (Rec4/17/5); two strains were composed of genotypes 1 and 17 (Rec1/17); and two strains were composed of genotype 1 and an undetermined strain (Rec1/und). Coalescent analysis based on the Vp1 gene, which is free of recombination, indicated that the recombinant strains most likely arose in this region approximately 30 years ago. They are present in high frequencies and are circulating in different small and isolated cities in the state of Tocantins. Further studies will be needed to establish whether the detected recombinant strains have been replacing parental strains or if they are co-circulating in distinct frequencies in Tocantins.
Élcio Leal; Adriana Luchs; Flávio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Danielle Elise Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cássia Vitória De Deus Alves Soares; Fabiola Villanova; Steven S. Witkin; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Antônio Charlys Da Costa. Recombinant Strains of Human Parechovirus in Rural Areas in the North of Brazil. Viruses 2019, 11, 488 .
AMA StyleÉlcio Leal, Adriana Luchs, Flávio Augusto De Pádua Milagres, Shirley Vasconcelos Komninakis, Danielle Elise Gill, Márcia Cristina Alves Brito Sayão Lobato, Rafael Brustulin, Rogério Togisaki Das Chagas, Maria De Fátima Neves Dos Santos Abrão, Cássia Vitória De Deus Alves Soares, Fabiola Villanova, Steven S. Witkin, Xutao Deng, Ester Cerdeira Sabino, Eric Delwart, Antônio Charlys Da Costa. Recombinant Strains of Human Parechovirus in Rural Areas in the North of Brazil. Viruses. 2019; 11 (6):488.
Chicago/Turabian StyleÉlcio Leal; Adriana Luchs; Flávio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Danielle Elise Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cássia Vitória De Deus Alves Soares; Fabiola Villanova; Steven S. Witkin; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Antônio Charlys Da Costa. 2019. "Recombinant Strains of Human Parechovirus in Rural Areas in the North of Brazil." Viruses 11, no. 6: 488.
The nearly complete genome sequences of two Cucumis melo endornavirus (CmEV) strains were obtained using deep sequencing while investigating fecal samples for the presence of gastroenteritis viruses. The Brazilian CmEV BRA/TO-23 (aa positions 116-5027) and BRA/TO-74 (aa positions 26-5057) strains were nearly identical to the reference CmEV CL-01 (USA) and SJ1 (South Korea) strains, showing 97% and 98% of nucleotide and amino acid identity, respectively. Endornaviruses are not known to be associated with human disease and their presence may simply reflect recent dietary consumption. Metagenomic analyses offered an opportunity to identify for the first time in Brazil a newly described endornavirus species.
Antonio Charlys Da Costa; Elcio Leal; Danielle Gill; Flavio Milagres; Shirley Vasconcelos Komninakis; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Márcia Cristina Alves Brito Sayão Lobato; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Adriana Luchs. Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil. Virus Genes 2019, 55, 332 -338.
AMA StyleAntonio Charlys Da Costa, Elcio Leal, Danielle Gill, Flavio Milagres, Shirley Vasconcelos Komninakis, Rafael Brustulin, Maria Da Aparecida Rodrigues Teles, Márcia Cristina Alves Brito Sayão Lobato, Rogério Togisaki Das Chagas, Maria De Fátima Neves Dos Santos Abrão, Cassia Vitória De Deus Alves Soares, Xutao Deng, Eric Delwart, Ester Cerdeira Sabino, Adriana Luchs. Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil. Virus Genes. 2019; 55 (3):332-338.
Chicago/Turabian StyleAntonio Charlys Da Costa; Elcio Leal; Danielle Gill; Flavio Milagres; Shirley Vasconcelos Komninakis; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Márcia Cristina Alves Brito Sayão Lobato; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Adriana Luchs. 2019. "Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil." Virus Genes 55, no. 3: 332-338.
Emergence of DS-1-like-G1P[8] rotavirus in Asia have been recently reported. We report for the first time the detection and the whole genome phylogenetic analysis of DS-1-like-G1P[8] strains in America. From 2013 to 2017, a total of 4226 fecal samples were screened for rotavirus by ELISA, PAGE, RT-PCR and sequencing. G1P[8] represented 3.7% (30/800) of all rotavirus-positive samples. DS-1-like-G1P[8] comprised 1.6% (13/800) detected exclusively in 2013, and Wa-like-G1P[8] comprised 2.1% (17/800) detected from 2013 to 2015. Whole genome sequencing confirmed the DS-1-like backbone I2-R2-C2-M2-A2-N2-T2-E2-H2. All genome segments of the Brazilian DS-1-like-G1P[8] strains clustered with those of Asian strains, and apart from African DS-1-like-G1P[8] strains. In addition, Brazilian DS-1-like-G1P[8] reassortants distantly clustered with DS-1-like backbone strains simultaneously circulating in the country, suggesting that the Brazilian DS-1-like-G1P[8] strains are likely imported from Asia. Two distinct NSP4 E2 genotype lineages were also identified, indicating the existence of a co-circulating pool of different DS-1-like G1P[8] strains. Surveillance systems must be developed to examine if RVA vaccines are still effective for the prevention against unusual DS-1-like-G1P[8] strains.
Adriana Luchs; Antonio Charlys Da Costa; Audrey Cilli; Shirley Cavalcante Vasconcelos Komninakis; Rita Carmona; Simone Guadagnucci Morillo; Ester Cerdeira Sabino; Maria Do Carmo Sampaio Tavares Timenetsky. First Detection of DS-1-like G1P[8] Double-gene Reassortant Rotavirus Strains on The American Continent, Brazil, 2013. Scientific Reports 2019, 9, 2210 .
AMA StyleAdriana Luchs, Antonio Charlys Da Costa, Audrey Cilli, Shirley Cavalcante Vasconcelos Komninakis, Rita Carmona, Simone Guadagnucci Morillo, Ester Cerdeira Sabino, Maria Do Carmo Sampaio Tavares Timenetsky. First Detection of DS-1-like G1P[8] Double-gene Reassortant Rotavirus Strains on The American Continent, Brazil, 2013. Scientific Reports. 2019; 9 (1):2210.
Chicago/Turabian StyleAdriana Luchs; Antonio Charlys Da Costa; Audrey Cilli; Shirley Cavalcante Vasconcelos Komninakis; Rita Carmona; Simone Guadagnucci Morillo; Ester Cerdeira Sabino; Maria Do Carmo Sampaio Tavares Timenetsky. 2019. "First Detection of DS-1-like G1P[8] Double-gene Reassortant Rotavirus Strains on The American Continent, Brazil, 2013." Scientific Reports 9, no. 1: 2210.
Human enteroviruses (EVs) are associated with a wide spectrum of human diseases. Here we report the complete genome sequences of one EV-C99 strain and one E29 strain obtained from children suffering from acute gastroenteritis, without symptoms of enteroviral syndromes. This is the first report of EV-C99 in South America, and the second E29 genome described worldwide. Continuous surveillance on EVs is vital to provide further understanding of the circulation of new or rare EV serotypes in the country. The present study also highlights the capacity of EVs to remain in silent circulation in populations.
Adriana Luchs; Elcio Leal; Kaelan Tardy; Flavio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Márcia Cristina Alves Brito Sayão Lobato; Rogério Togisaki das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Antonio Charlys da Costa. The rare enterovirus c99 and echovirus 29 strains in Brazil: potential risks associated to silent circulation. Memórias do Instituto Oswaldo Cruz 2019, 114, e190160 .
AMA StyleAdriana Luchs, Elcio Leal, Kaelan Tardy, Flavio Augusto De Pádua Milagres, Shirley Vasconcelos Komninakis, Rafael Brustulin, Maria Da Aparecida Rodrigues Teles, Márcia Cristina Alves Brito Sayão Lobato, Rogério Togisaki das Chagas, Maria De Fátima Neves Dos Santos Abrão, Cassia Vitória De Deus Alves Soares, Xutao Deng, Eric Delwart, Ester Cerdeira Sabino, Antonio Charlys da Costa. The rare enterovirus c99 and echovirus 29 strains in Brazil: potential risks associated to silent circulation. Memórias do Instituto Oswaldo Cruz. 2019; 114 ():e190160.
Chicago/Turabian StyleAdriana Luchs; Elcio Leal; Kaelan Tardy; Flavio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Márcia Cristina Alves Brito Sayão Lobato; Rogério Togisaki das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Antonio Charlys da Costa. 2019. "The rare enterovirus c99 and echovirus 29 strains in Brazil: potential risks associated to silent circulation." Memórias do Instituto Oswaldo Cruz 114, no. : e190160.
Enterovirus B73 is a new member of the Enterovirus B species. First detected in the USA, it has been subsequently identified in China, India, Oman, and the Netherlands. In this study, we characterize the first B73 strain (named TO-127) to be detected in South America. TO-127 was obtained from a child with acute gastroenteritis living in a rural area in Northern Brazil. The subject was not infected with any known enteric pathogens such as norovirus, rotavirus, helminths, or enteric bacteria. Analysis of the nearly full-length TO-127 genome (6993 nt) indicated a 74–75% nucleotide similarity with EV-B73 strains from other countries. Evolutionary analysis suggests that B73 is endemic and widespread.
Geovani De Oliveira Ribeiro; Adriana Luchs; Flávio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Danielle Elise Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Steven S. Witkin; Fabiola Villanova; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Antonio Charlys Da Costa; Élcio Leal. Detection and Characterization of Enterovirus B73 from a Child in Brazil. Viruses 2018, 11, 16 .
AMA StyleGeovani De Oliveira Ribeiro, Adriana Luchs, Flávio Augusto De Pádua Milagres, Shirley Vasconcelos Komninakis, Danielle Elise Gill, Márcia Cristina Alves Brito Sayão Lobato, Rafael Brustulin, Rogério Togisaki Das Chagas, Maria De Fátima Neves Dos Santos Abrão, Cassia Vitória De Deus Alves Soares, Steven S. Witkin, Fabiola Villanova, Xutao Deng, Ester Cerdeira Sabino, Eric Delwart, Antonio Charlys Da Costa, Élcio Leal. Detection and Characterization of Enterovirus B73 from a Child in Brazil. Viruses. 2018; 11 (1):16.
Chicago/Turabian StyleGeovani De Oliveira Ribeiro; Adriana Luchs; Flávio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Danielle Elise Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Steven S. Witkin; Fabiola Villanova; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Antonio Charlys Da Costa; Élcio Leal. 2018. "Detection and Characterization of Enterovirus B73 from a Child in Brazil." Viruses 11, no. 1: 16.
The present investigation does not suggest that HAdV has assumed an epidemiological importance in Brazil after the RVA vaccine introduction and contributed to the definition of the clinical and public health significance of HAdV infections.
Dieli Primo; Gabriela Tayna Pacheco; Maria Do Carmo Sampaio Tavares Timenetsky; Adriana Luchs. Surveillance and molecular characterization of human adenovirus in patients with acute gastroenteritis in the era of rotavirus vaccine, Brazil, 2012–2017. Journal of Clinical Virology 2018, 109, 35 -40.
AMA StyleDieli Primo, Gabriela Tayna Pacheco, Maria Do Carmo Sampaio Tavares Timenetsky, Adriana Luchs. Surveillance and molecular characterization of human adenovirus in patients with acute gastroenteritis in the era of rotavirus vaccine, Brazil, 2012–2017. Journal of Clinical Virology. 2018; 109 ():35-40.
Chicago/Turabian StyleDieli Primo; Gabriela Tayna Pacheco; Maria Do Carmo Sampaio Tavares Timenetsky; Adriana Luchs. 2018. "Surveillance and molecular characterization of human adenovirus in patients with acute gastroenteritis in the era of rotavirus vaccine, Brazil, 2012–2017." Journal of Clinical Virology 109, no. : 35-40.
Here we report the nearly full-length genome of a recombinant Saffold virus strain (SAFV-BR-193) isolated from a child with acute gastroenteritis. Evolutionary analysis performed using all available near-full length Saffold picornavirus genomes showed that the breakpoint found in the Brazilian strain (SAFV-BR-193) is indeed a recombination hotspot. Notably, this hotspot is located just one nucleotide after the ribosomal frameshift GGUUUUU motif in the SAFV genome. Empirical studies will be necessary to determine if this motif also affects the binding affinity of RNA-dependent RNA-polymerase (RdRp) and therefore increases the changes of RdRp swap between molecules during the synthesis of viral genomes.
Antônio Charlys Da Costa; Adriana Luchs; Flávio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Danielle Elise Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Élcio Leal. Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus. Viruses 2018, 10, 520 .
AMA StyleAntônio Charlys Da Costa, Adriana Luchs, Flávio Augusto De Pádua Milagres, Shirley Vasconcelos Komninakis, Danielle Elise Gill, Márcia Cristina Alves Brito Sayão Lobato, Rafael Brustulin, Rogério Togisaki Das Chagas, Maria De Fátima Neves Dos Santos Abrão, Cassia Vitória De Deus Alves Soares, Xutao Deng, Ester Cerdeira Sabino, Eric Delwart, Élcio Leal. Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus. Viruses. 2018; 10 (10):520.
Chicago/Turabian StyleAntônio Charlys Da Costa; Adriana Luchs; Flávio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Danielle Elise Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Élcio Leal. 2018. "Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus." Viruses 10, no. 10: 520.
In the present article we report the nearly full length genome of a Cosavirus strain (BRTO-83) isolated from a child with acute gastroenteritis, and who is an inhabitant of a rural area in the central region of Brazil. The sample was previously screened and negative for both: common enteric viruses (i.e. rotavirus and norovirus), bacteria, endoparasites and helminthes. Evolutionary analysis and phylogenetic inferences indicated that the Brazilian BRTO-83 Cosavirus strain was a recombinant virus highly related to the E/D recombinant NG385 strain (Genbank JN867757), which was isolated in Nigeria from an acute flaccid paralysis patient. This is the first report of a recombinant E/D Cosavirus strain detected in Brazil, and the second genome described worldwide. Further surveillance and molecular studies are required to fully understand the epidemiology, distribution and evolution of the Cosavirus.
Antonio Charlys Da Costa; Adriana Luchs; Flavio Milagres; Shirley Vasconcelos Komninakis; Danielle Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Élcio Leal. Near full length genome of a recombinant (E/D) cosavirus strain from a rural area in the central region of Brazil. Scientific Reports 2018, 8, 1 -5.
AMA StyleAntonio Charlys Da Costa, Adriana Luchs, Flavio Milagres, Shirley Vasconcelos Komninakis, Danielle Gill, Márcia Cristina Alves Brito Sayão Lobato, Rafael Brustulin, Rogério Togisaki Das Chagas, Maria De Fátima Neves Dos Santos Abrão, Cassia Vitória De Deus Alves Soares, Xutao Deng, Ester Cerdeira Sabino, Eric Delwart, Élcio Leal. Near full length genome of a recombinant (E/D) cosavirus strain from a rural area in the central region of Brazil. Scientific Reports. 2018; 8 (1):1-5.
Chicago/Turabian StyleAntonio Charlys Da Costa; Adriana Luchs; Flavio Milagres; Shirley Vasconcelos Komninakis; Danielle Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Élcio Leal. 2018. "Near full length genome of a recombinant (E/D) cosavirus strain from a rural area in the central region of Brazil." Scientific Reports 8, no. 1: 1-5.
We report here the complete genome sequence of a bipartite virus, herein denoted WLPRV/human/BRA/TO-34/201, from a sample collected in 2015 from a two-year-old child in Brazil presenting acute gastroenteritis. The virus has 98-99% identity (segments 2 and 1, respectively) with the Wuhan large pig roundworm virus (unclassified RNA virus) that was recently discovered in the stomachs of pigs from China. This is the first report of a Wuhan large pig roundworm virus detected in human specimens, and the second genome described worldwide. However, the generation of more sequence data and further functional studies are required to fully understand the ecology, epidemiology, and evolution of this new unclassified virus.
Adriana Luchs; Elcio Leal; Shirley Vasconcelos Komninakis; Flavio Milagres; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Danielle Gill; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Antonio Charlys Da Costa. Wuhan large pig roundworm virus identified in human feces in Brazil. Virus Genes 2018, 54, 470 -473.
AMA StyleAdriana Luchs, Elcio Leal, Shirley Vasconcelos Komninakis, Flavio Milagres, Rafael Brustulin, Maria Da Aparecida Rodrigues Teles, Danielle Gill, Xutao Deng, Eric Delwart, Ester Cerdeira Sabino, Antonio Charlys Da Costa. Wuhan large pig roundworm virus identified in human feces in Brazil. Virus Genes. 2018; 54 (3):470-473.
Chicago/Turabian StyleAdriana Luchs; Elcio Leal; Shirley Vasconcelos Komninakis; Flavio Milagres; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Danielle Gill; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Antonio Charlys Da Costa. 2018. "Wuhan large pig roundworm virus identified in human feces in Brazil." Virus Genes 54, no. 3: 470-473.
The aims of this study were to investigate the human bocavirus (HBoV) frequency and genotypes in hospitalized children <5 years presenting acute respiratory infections (ARI) within the São Paulo metropolitan area. Nasopharyngeal samples from 300 patients, previously screened for common respiratory viruses, were tested by qPCR for the NSP1 and NP-1 genes. The VP1/2 gene in positive samples was then amplified by PCR and sequenced. A total of 49 positive HBoV cases (16.3%; mean Ct value of 34.41) were detected with the mean age being 18.1 months (range 1 month to 5 years) and the median age being 1 year of age. Children aged between 0 and 12 months had higher detection rates of HBoV (69.4%; 34/49; mean Ct = 34.45) than children from other age groups (30.6%; 15/49; mean Ct = 34.34). No significant differences were observed between HBoV Ct levels and clinical illness. The occurrence was more frequently associated with fall (38.8%; 19/49) and spring (36.7%; 18/49). All 12 sequenced isolates were identified as HBoV-1, displaying minor genetic variation compared to the Swedish reference strains ST1 and ST2 (99.1–99.7% nt). The sole identification of HBoV-1 supports the hypothesis that this particular genotype is strongly related to ARI, and contributes to the role of this virus in the aetiology of respiratory diseases.
Patrícia Evelin Silva; Cristina Adelaide Figueiredo; Adriana Luchs; Terezinha Maria De Paiva; Margarete Aparecida Benega Pinho; Renato Sousa Paulino; Daniela Bernardes Borges Da Silva; Katia Correa De Oliveira Santos; Ana Maria Sardinha Afonso; Maria Isabel De Oliveira. Human bocavirus in hospitalized children under 5 years with acute respiratory infection, São Paulo, Brazil, 2010. Archives of Virology 2018, 163, 1325 -1330.
AMA StylePatrícia Evelin Silva, Cristina Adelaide Figueiredo, Adriana Luchs, Terezinha Maria De Paiva, Margarete Aparecida Benega Pinho, Renato Sousa Paulino, Daniela Bernardes Borges Da Silva, Katia Correa De Oliveira Santos, Ana Maria Sardinha Afonso, Maria Isabel De Oliveira. Human bocavirus in hospitalized children under 5 years with acute respiratory infection, São Paulo, Brazil, 2010. Archives of Virology. 2018; 163 (5):1325-1330.
Chicago/Turabian StylePatrícia Evelin Silva; Cristina Adelaide Figueiredo; Adriana Luchs; Terezinha Maria De Paiva; Margarete Aparecida Benega Pinho; Renato Sousa Paulino; Daniela Bernardes Borges Da Silva; Katia Correa De Oliveira Santos; Ana Maria Sardinha Afonso; Maria Isabel De Oliveira. 2018. "Human bocavirus in hospitalized children under 5 years with acute respiratory infection, São Paulo, Brazil, 2010." Archives of Virology 163, no. 5: 1325-1330.
The aims of this study were to monitor human astrovirus (HAstV) infections in patients presenting with acute gastroenteritis in Brazil and to determine the HAstV genotypes of these viruses. From May 2010 to July 2012, a total of 140 samples that were negative for both rotaviruses and noroviruses were randomly selected and tested for the presence of HAstV using an RT-PCR assay specific for the ORF2 region. Viral genotypes were identified and genetic diversity was investigated by sequencing. HAstV infection was detected in 2.9% of samples (4/140). The viruses in three samples were shown by phylogenetic analysis to belong to HAstV-4 lineage “c”, clustering together with strains detected in Europe and the Middle East. The virus in one sample was genotyped as HAstV-1 lineage “a”, clustering with strains from Uruguay, Brazil and Russia. Our findings provide further evidence for a global distribution of HAstV-1a and suggest a possible emergent importance of the HAstV-4c lineage in this country. The present study does not suggest that HAstVs currently have a major epidemiological impact, even after the introduction of a rotavirus vaccine in 2006.
Simone Guadagnucci Morillo; Adriana Luchs; Audrey Cilli; Rita Carmona; Maria Do Carmo S. T. Timenetsky. Detection and genetic characterization of classic human astroviruses in Brazil, 2010-2012. Archives of Virology 2018, 163, 1293 -1297.
AMA StyleSimone Guadagnucci Morillo, Adriana Luchs, Audrey Cilli, Rita Carmona, Maria Do Carmo S. T. Timenetsky. Detection and genetic characterization of classic human astroviruses in Brazil, 2010-2012. Archives of Virology. 2018; 163 (5):1293-1297.
Chicago/Turabian StyleSimone Guadagnucci Morillo; Adriana Luchs; Audrey Cilli; Rita Carmona; Maria Do Carmo S. T. Timenetsky. 2018. "Detection and genetic characterization of classic human astroviruses in Brazil, 2010-2012." Archives of Virology 163, no. 5: 1293-1297.
The present study described a group A rotavirus (RVA) outbreak in an age-care facility in Brazil, using epidemiologic and molecular diagnostic methods. A descriptive clinical, epidemiological and environmental investigation was conducted. Stool samples were collected and screened for RVA, Norovirus (NoV), Enteric Adenovirus 40/41 (AdV 40/41) and Astrovirus (AstV) using ELISA, RT-PCR, qRT-PCR, electron microscopy and sequencing methods. Outbreak occurred during 26th-29th October, 2015; 28 individuals affected (22 residents; 6 staff). The attack rate was 25.9% and 8.5% among residents (median-age: 85.5 years) and staff (median-age: 28 years), respectively. Female staff was identified as the index case. RVA G2P[4] genotype was detected in 87.5% (7/8). Genetic analysis demonstrated that the outbreak involved one single strain, suggesting a common-source infection. RVA should be considered during outbreaks investigations in residential facilities, and raise the question if the current licensed RVA vaccines for children could also be helpful for the elderly.
Adriana Luchs; Geraldine Madalosso; Audrey Cilli; Simone Guadagnucci Morillo; Sandra Regina Martins; Karen Aparecida Farias De Souza; Gislene Mitsue Namiyama; Cláudia Regina Gonçalves; Rita Carmona; Maria Do Carmo Sampaio Tavares Timenetsky. Outbreak of G2P[4] rotavirus gastroenteritis in a retirement community, Brazil, 2015: An important public health risk? Geriatric Nursing 2017, 38, 283 -290.
AMA StyleAdriana Luchs, Geraldine Madalosso, Audrey Cilli, Simone Guadagnucci Morillo, Sandra Regina Martins, Karen Aparecida Farias De Souza, Gislene Mitsue Namiyama, Cláudia Regina Gonçalves, Rita Carmona, Maria Do Carmo Sampaio Tavares Timenetsky. Outbreak of G2P[4] rotavirus gastroenteritis in a retirement community, Brazil, 2015: An important public health risk? Geriatric Nursing. 2017; 38 (4):283-290.
Chicago/Turabian StyleAdriana Luchs; Geraldine Madalosso; Audrey Cilli; Simone Guadagnucci Morillo; Sandra Regina Martins; Karen Aparecida Farias De Souza; Gislene Mitsue Namiyama; Cláudia Regina Gonçalves; Rita Carmona; Maria Do Carmo Sampaio Tavares Timenetsky. 2017. "Outbreak of G2P[4] rotavirus gastroenteritis in a retirement community, Brazil, 2015: An important public health risk?" Geriatric Nursing 38, no. 4: 283-290.