Salinity stress significantly hampers cotton seed germination and seedling growth. Employing plant growth regulators stands out as an effective strategy to mitigate salt stress. In this study, we assessed the impact of varying concentrations of natural composite salt conditions (0%, 0.6%, and 1.2%) on cotton seed germination, seedling growth, and physiology. Additionally, we explored the effects of compound sodium nitrophenolate (CSN: 2 mg·L⁻¹ and 10 mg·L⁻¹), 24-epibrassinolide (EBR: 0.02 mg·L⁻¹ and 0.1 mg·L⁻¹), and gibberellic acid (GA: 60 mg·L⁻¹ and 300 mg·L⁻¹), against a control (CK: distilled water) group. The results indicate that with an increase in the composite salt concentration, the germination potential (GP) and germination rate (GR) of cotton seeds gradually decrease. Simultaneously, the fresh weight and root vitality of seedlings also correspondingly decrease, while the degree of membrane lipid peroxidation increases. Under high-salt (1.2%) conditions, soaking treatments with CSN and EBR significantly enhance both GP (45–59% and 55–64%) and GR (30–33% and 39–36%) compared to the CK. However, the GA treatment does not increase the GP and GR of cotton. Moreover, under high-salt (1.2%) conditions, CSN and EBR treatments result in the increased activities of superoxide dismutase (56–66% and 71–80%), peroxidase (20–24% and 37–51%), and catalase (26–32% and 35–46%). Consequently, cotton exhibits a relatively good tolerance to natural composite salts. Soaking treatments with CSN and EBR effectively improve cotton germination by enhancing antioxidant enzyme activities, thereby strengthening cotton’s tolerance to salt stress. These findings offer new insights for enhancing the salt tolerance of cotton.

Excessive salt content in soil has adverse effects on cotton production, especially during the germination and seedling stages. γ-aminobutyric acid (GABA) is an important active substance that is expected to improve the resistance of plants to abiotic stresses. This study focused on two cotton cultivars (Gossypium hirsutum L.: Tahe 2 and Xinluzhong 62) and investigated the impact of exogenous GABA (0, 1, 2, 3, and 4 mM) on seed germination, seedling growth, and related morphological, physiological, and biochemical indicators under salt stress (150 mM NaCl). The results showed that salt stress significantly reduced the germination rate and germination index of cotton seeds (decreased by 20.34% and 32.14% for Tahe 2 and Xinluzhong 62, respectively), leading to decreased seedling height and biomass and causing leaf yellowing. Salt stress induced osmotic stress in seedlings, resulting in ion imbalance (marked reduction in K⁺/Na⁺ ratio) and oxidative damage. Under salt stress conditions, exogenous GABA increased the germination rate (increased by 10.64~23.40% and 2.63~31.58% for Tahe 2 and Xinluzhong 62, respectively) and germination index of cotton seeds, as well as plant height and biomass. GABA treatment improved leaf yellowing. Exogenous GABA treatment increased the content of proline and soluble sugars, with varying effects on betaine. Exogenous GABA treatment reduced the Na⁺ content in seedlings, increased the K⁺ content, and increased the K⁺/Na⁺ ratio (increased by 20.44~28.08% and 29.54~76.33% for Tahe 2 and Xinluzhong 62, respectively). Exogenous GABA treatment enhanced the activities of superoxide dismutase and peroxidase, and reduced the accumulation of hydrogen peroxide and malondialdehyde, but had a negative impact on catalase activity. In conclusion, exogenous GABA effectively improved cotton seed germination. By regulating osmoprotectant levels, maintaining ion homeostasis, and alleviating oxidative stress, GABA mitigated the adverse effects of salt stress on cotton seedling growth.

Soil salinization is a primary environmental factor leading to reduced crop yields, and oil shale waste residues may have the potential to alleviate plant salt stress. This study aims to investigate the effects of three types of oil shale waste residues (fine concentrate ore, fine ore, and semi-coke) on the growth and physiological characteristics of maize seedlings in saline–alkali soil. The results indicate the following: (1) All three types of oil shale waste residues increased the root vitality of seedlings and reduced the root proline content. (2) The three types of oil shale waste residues increased the activity of superoxide dismutase (1.70% to 97.19%) and peroxidase (29.39% to 61.21%) in maize seedlings, but there were differences in their effects on catalase activity. The fine ore and semi-coke treatments increased catalase activity (4.98% to 77.42%), while fine concentrate ore decreased catalase activity (39.28% to 5.30%). (3) The three types of oil shale waste residues effectively alleviated the degree of membrane lipid peroxidation in maize seedling leaves. (4) Principal component analysis showed that the semi-coke treatment was beneficial to the growth and physiology of maize seedlings in saline–alkali soil, with the optimal effect occurring at a 0.2% addition rate. In conclusion, adding semi-coke to saline–alkali soil promotes the growth of maize by regulating its physiological and biochemical mechanisms, alleviating the salt stress on maize seedlings caused by salt content.