There are numerous reports of animals being struck by lightning, but the number of science-oriented studies in which a necropsy is performed, and the findings are documented, is relatively few and mostly focused on mammals. In contrast, analogous information about birds is scarce. The purpose of this article is to present the findings after a postmortem examination of an 85–90-day-old Eurasian Griffon Vulture (Gyps fulvus) that was most likely killed by lightning while in the nest in Sinite Kamani Nature Park in Bulgaria in 2021. The body was visibly burned, with keraunographic markings, lacked feathers and had fractured bones—typical findings in lightning strike injuries.

The globally endangered saker falcon (Falco cherrug) is currently being re-introduced in Bulgaria, where the falcons are bred in captivity and released through the hacking method. We relied on the birds’ pedigree when forming the breeding pairs from 2011. In 2021-2022, we had the opportunity to evaluate our captive population via DNA tests. We performed the first genetic assessment of the sakers in the WRBC through a genome evaluation of the most important founders (n = 12) and, in 2022, we executed a microsatellite analysis on 30 saker falcons from the programme. We compared the results with the known pedigree and history of the saker falcons. The genetic tests helped to assign relatedness to some birds with missing or incomplete pedigrees, indicating the test can complement that information and lead to better management of the captive group. One pair was separated as a precaution as it was indicated by one the tests that the two birds are more closely related than expected. The research could be beneficial to other raptor captive breeding programmes dealing with a similar group composition.

Tomamos muestras de sangre de 44 individuos de Falco cherrug cherrug y de 68 individuos de Falco cherrug milvipes en cautividad en dos instalaciones en Bulgaria para evaluar la salud de los individuos involucrados en un proyecto de reintroducción, y para obtener información sobre los procedimientos de cuidado veterinario y de tratamiento de las aves. Comparamos los valores de 18 parámetros bioquímicos entre las dos subespecies y también entre hembras y machos. Encontramos diferencias significativas entre las dos subespecies para algunos de los parámetros evaluados; sin embargo, probablemente fueron el resultado de diferentes regímenes de alimentación en las dos instalaciones de cría: una albergaba a la mayoría de los individuos de F. c. cherrug y la otra a todos los individuos de F. c. milvipes. Las evaluaciones adicionales deberían comparar los valores bioquímicos entre las aves de las dos subespecies alojadas en la misma instalación. También encontramos diferencias significativas en la bioquímica de individuos machos y hembras en algunos parámetros probados. Después de 20 años sin registros de nidificación de F. cherrug en Bulgaria, esta especie en peligro de extinción a nivel mundial ha estado reproduciéndose exitosamente allí desde 2018 como resultado de un proyecto de reintroducción en curso. Al establecer información de base sobre las diferencias en la bioquímica de F. cherrug, nuestra investigación puede guiar los estudios futuros de esta rara especie y ayudar al cuidado veterinario y al tratamiento de las aves que se reproducen en cautividad. [Traducción del equipo editorial]

After a massive decline, the Griffon Vulture (Gyps fulvus) population in Bulgaria has now stabilised as a result of the hard work of conservation programmes, although it is still listed as an Endangered species (EN) in the country's Red Data Book. Due to a series of unfortunate events, another species - the Cinereous Vulture (Aegypius monachus) became extinct in Bulgaria and recovered only recently due to systematic re-introduction efforts. Along with the poor living conditions, a predisposing factor for the decreasing population of the vultures is the fact they hatch only a single egg; two may be laid on exceptions. The survival rate of the young is from medium to low. In that order, a method of double clutching has been applied in the hope of preserving and retrieving both of the vultures. This article aims to introduce the double clutching method in detail, as well as to show the results of its implementation into the breeding programme of the Cinereous Vulture and the Griffon Vulture at the Wildlife Rehabilitation and Breeding Centre of Green Balkans – Stara Zagora NGO. This research studies the time period 2019-2023. The purpose of this study is to compare the results of natural copulation and hatching and those of the double clutching method. The data taken are used for studies for both the Cinereous and the Griffon Vulture’s reproduction in correlation with their feeding programme and living conditions. The results of the study will be summarised into the following text for the purposes of shedding light on the positives of the double clutching method.

Two subspecies of Saker Falcon are commonly accepted - Western (Falco cherrug cherrug) and Eastern (Falco cherrug milvipes), which are differentiated by their distribution range and phenotype. In Bulgaria, Western Saker Falcons are breeding ex situ in the Wildlife Rehabilitation and Breeding Centre, part of Green Balkans - Stara Zagora NGO, with the aim of restoring the nesting population of the species in the country and both Western and Eastern - in the Breeding Centre for Birds of Prey in Burgas for the purpose of sale for the needs of falconry in the country and abroad. In 2021, a total of 115 birds from the two breeding centres were sampled. The samples were analysed in Bielefeld University (Germany) at nine microsatellite loci. Structure analyses were performed to establish the optimal explanatory number of groups. We compared the putative genetic groups with the known/expected origin of falcons. A separation in two groups best explained the allelic variation between samples. Out of 68 Saker Falcons with putatively Eastern origin, 66 were ascribed to genetic group 2 and two falcons had unclear, mixed or hybrid genetic fingerprints. Out of 42 Sakers with putatively Western origin, 33 were ascribed to genetic group 1, seven to genetic group 2 and two individuals appeared to have a mixed signature of genetic groups 1 and 2 with dominating alleles of group 2. Five known hybrids were scored as mixed signature with dominating genetic cluster 2. This suggests that the two (Eastern and Western) populations of Saker Falcon origin suggested by the subspecies' definitions are also adequate to be considered in breeding programmes. Genetic cluster 1 might represent the ancestral alleles shared with other falcons, while specific novel alleles allow the discrimination of secured Eastern Sakers (group 2), while these populations may be occasionally invaded by individuals from the west.

This study presents preliminary data on the diet composition of reintroduced Lesser Kestrels (Falco naumanni) in two restored colonies in Bulgaria—near Stara Zagora and in the Sakar Mountains at Levka village—based on pellet analysis. A total of 103 and 48 identifiable prey items (occurrences in the pellets) were recovered from the respective sites. Insects (particularly Coleoptera) dominated the diet at both colonies, but some differences were observed in the occurrence of vertebrate prey. Birds (Aves) were the second most common category in Stara Zagora, while small mammals (Mammalia) were more prevalent in Sakar. Annelids were detected only in Stara Zagora, and millipedes (Diplopoda) only in Sakar. Synthetic materials were found in pellets from Stara Zagora, suggesting plastic ingestion. Levins’ index indicated similar niche breadths (1.915 vs. 2.008), and Pianka’s index showed a high dietary overlap (0.981), pointing to broadly similar trophic preferences despite local prey availability.