The aim of the present study was to explore heavy metal tolerance and accumulation potential in Anthyllis vulneraria subsp. maritima plants from coastal sand dunes in controlled conditions. Plants were established from seeds collected in coastal sand dunes and cultivated in substrates in greenhouse conditions. A gradual treatment with CdCl₂, PbOAc, CuSO₄, MnSO₄, and ZnSO₄ was performed until three final concentrations for each metal were reached. The number of leaves, their biomass, and biomass of roots were negatively affected by increasing concentrations of lead (Pb) and manganese (Mn) in substrate, but no negative effect was evident for cadmium (Cd), copper (Cu), and zinc (Zn). Visible effects of metal toxicity were evident for Pb-treated plants (appearance of thinner leaves, yellowing of older leaves), as well as for Mn-treated plants (reduced leaf size, curled leaves, red leaf venation). There was a significant decrease in water content in old leaves at high Pb and increasing Mn concentration, indicating accelerated leaf senescence. Increase in polyphenol oxidase activity in leaves was evident in all the plants treated with heavy metals. In contrast, an increase in peroxidase activity was evident only for plants treated with 50 and 100 mg L⁻¹ Cd, 500 mg L⁻¹ Pb, 200–1000 mg L⁻¹ Mn, and 500 mg L⁻¹ Zn. Metal accumulation potential for Cd and Cu was the highest in the roots, but for Pb, Mn, and Zn, more metal accumulated in old leaves. It can be concluded that A. vulneraria subsp. maritima plants are tolerant to high Cd, Cu, and Zn, but moderately susceptible to Pb and Mn. However, oxidative enzyme activity cannot be unequivocally used as a specific indicator of metal tolerance. In respect to phytoremediation potential, the plants have very good accumulation capacity for Pb, Mn, and Zn.

The aim of the present study was to assess heavy metal tolerance and its accumulation potential in coastal nitrophilic species Rumex maritimus, and to study the possible effects of nitrogen fertilizer and salinity on the characteristics of metal-treated plants. Two experiments were performed in partially controlled greenhouse conditions: (1) gradual treatment with increasing concentrations of Cd, Pb, Cu, Mn, and Zn; and (2) acute treatment with Cd, Pb, and Cu on the background of different nitrogen fertilizer amendment rates (0.15 and 0.30 g L⁻¹ N) and salinity (50 and 100 mM). R. maritimus plants were extremely tolerant to treatment with all metals, with no negative effect on total leaf biomass both in the case of gradual or acute treatment. However, the number and biomass of dry leaves increased under high doses of heavy metals, and the effect was more pronounced in the case of acute treatment. All studied metals were excluded from the roots and young leaves, predominantly accumulating in the dry leaves, reaching 250 mg kg⁻¹ for Cd, 2000 mg kg⁻¹ for Pb, and 500 mg kg⁻¹ for Cu. In the second experiment, the presence of increased nitrogen in the substrate positively affected the growth of R. maritimus plants and their morphological response to heavy metals, but salinity affected metal accumulation. Photosynthesis-related parameters, leaf chlorophyll concentration, and the chlorophyll a fluorescence parameter Performance Index Total confirmed that heavy metals had no negative effect on the physiological state of photosynthetically active leaves. It is concluded that R. maritimus plants have exceptional potential for practical phytoremediation needs due to the high tolerance and accumulation potential for heavy metals.

This study focuses on the endangered neo-endemic Baltic dunes species Linaria loeselii Schweigg. (Plantaginaceae), also known as Linaria odora (M. Bieb.). By utilizing in vitro cultures, we successfully germinated seeds collected in situ. Our method, which involved using media supplemented with 5 µmol/L 6-benzylaminopurine, led to the indirect regeneration of shoots after 60 days of culture in the dark, significantly increasing the number of progeny plants. Additionally, the medium supplemented with 2.85 μmol/L indole-3-acetic acid and 10.2 μmol/L paclobutrazol allowed rooting after 30 days of shoot fragments. This research provides a potential basis for developing Linaria loeselii introduction programs into the environment, thereby contributing to the conservation of this endangered species.

Ex situ collections of rare and endangered plant species are crucial components of integrated conservation systems, as outlined in the Global Strategy for Plant Conservation. Plant tissue culture collections play an essential role in achieving conservation objectives, as they offer a means of propagating plant material for habitat restoration and other practical applications. This study analyzes existing tissue culture protocols for coastal plant species of the Baltic Sea region, reviewing micropropagation research spanning 25 families and 112 highly coastal-specific species. The analysis of 232 experimental studies showed that tissue culture studies have been conducted with 38 coastal-specific species of the Baltic Sea region, which is only 34% of the initially identified target species. Special attention in the field of tissue culture technology development should be paid to several highly coastal-specific species with great potential for practical use, such as Blysmus rufus, Bolboschoenus maritimus, Schoenoplectus tabernaemontani, Halimione spp., Cochlearia spp., Euphrasia spp., and Odontites spp. Comprehensive research on tissue cultures of coastal plant species is imperative to establish micropropagation protocols for underrepresented taxonomic groups. The research should prioritize functional studies with ecological relevance and the development of practical biotechnological approaches for commercial applications.

Gatunki roślin wydmowych są narażone na wiele zagrożeń, zarówno naturalnych, jak i spowodowanych przez człowieka. Zmiany klimatu skutkują erozją wydm i zaburzeniami w naturalnej sukcesji tych ekosystemów. Do czynników antropogenicznych należą m.in. wprowadzanie gatunków obcych, nadmierna eksploatacja turystyczna oraz urbanizacja. Celem artykułu jest przedstawienie zagrożeń i form ochrony gatunków roślin wydmowych regionu bałtyckiego. Gatunki te pełnią kluczową rolę w utrzymaniu stabilności wydm i różnorodności biologicznej. Przedstawione zostały główne czynniki wpływające na degradację roślinności wydmowej, takie jak erozja, zanieczyszczenie środowiska, inwazja obcych gatunków, zmiany klimatu i presja turystyczna oraz możliwe sposoby ochrony tych gatunków roślin, opierając się na przykładach z różnych krajów nadbałtyckich. Artykuł ma charakter przeglądowy i podkreśla potrzebę współpracy międzynarodowej w tej dziedzinie.