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Dr. Emmanuel Panteris
Aristotle University of Thessaloniki, School of Biology, Department of Botany, Thessaloniki, Greece

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0 Cytoskeleton
0 development
0 morphogenesis
0 cell division
0 cell wall

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Microtubules
cell wall
Cytoskeleton
cell division
cytokinesis
F-actin
morphogenesis
development
mitosis

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Journal article
Published: 03 June 2021 in Journal of Biological Research-Thessaloniki
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Background Cortical microtubules regulate cell expansion by determining cellulose microfibril orientation in the root apex of Arabidopsis thaliana. While the regulation of cell wall properties by cortical microtubules is well studied, the data on the influence of cell wall to cortical microtubule organization and stability remain scarce. Studies on cellulose biosynthesis mutants revealed that cortical microtubules depend on Cellulose Synthase A (CESA) function and/or cell expansion. Furthermore, it has been reported that cortical microtubules in cellulose-deficient mutants are hypersensitive to oryzalin. In this work, the persistence of cortical microtubules against anti-microtubule treatment was thoroughly studied in the roots of several cesa mutants, namely thanatos, mre1, any1, prc1-1 and rsw1, and the Cellulose Synthase Interacting 1 protein (csi1) mutant pom2-4. In addition, various treatments with drugs affecting cell expansion were performed on wild-type roots. Whole mount tubulin immunolabeling was applied in the above roots and observations were performed by confocal microscopy. Results Cortical microtubules in all mutants showed statistically significant increased persistence against anti-microtubule drugs, compared to those of the wild-type. Furthermore, to examine if the enhanced stability of cortical microtubules was due to reduced cellulose biosynthesis or to suppression of cell expansion, treatments of wild-type roots with 2,6-dichlorobenzonitrile (DCB) and Congo red were performed. After these treatments, cortical microtubules appeared more resistant to oryzalin, than in the control. Conclusions According to these findings, it may be concluded that inhibition of cell expansion, irrespective of the cause, results in increased microtubule stability in A. thaliana root. In addition, cell expansion does not only rely on cortical microtubule orientation but also plays a regulatory role in microtubule dynamics, as well. Various hypotheses may explain the increased cortical microtubule stability under decreased cell expansion such as the role of cell wall sensors and the presence of less dynamic cortical microtubules.

ACS Style

Veronica Giourieva; Emmanuel Panteris. Inhibition of cell expansion enhances cortical microtubule stability in the root apex of Arabidopsis thaliana. Journal of Biological Research-Thessaloniki 2021, 28, 1 -16.

AMA Style

Veronica Giourieva, Emmanuel Panteris. Inhibition of cell expansion enhances cortical microtubule stability in the root apex of Arabidopsis thaliana. Journal of Biological Research-Thessaloniki. 2021; 28 (1):1-16.

Chicago/Turabian Style

Veronica Giourieva; Emmanuel Panteris. 2021. "Inhibition of cell expansion enhances cortical microtubule stability in the root apex of Arabidopsis thaliana." Journal of Biological Research-Thessaloniki 28, no. 1: 1-16.

Journal article
Published: 18 May 2021 in Molecules
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We evaluated photosystem II (PSII) functionality in potato plants (Solanum tuberosum L.) before and after a 15 min feeding by the leaf miner Tuta absoluta using chlorophyll a fluorescence imaging analysis combined with reactive oxygen species (ROS) detection. Fifteen minutes after feeding, we observed at the feeding zone and at the whole leaf a decrease in the effective quantum yield of photosystem II (PSII) photochemistry (ΦPSII). While at the feeding zone the quantum yield of regulated non-photochemical energy loss in PSII (ΦNPQ) did not change, at the whole leaf level there was a significant increase. As a result, at the feeding zone a significant increase in the quantum yield of non-regulated energy loss in PSII (ΦNO) occurred, but there was no change at the whole leaf level compared to that before feeding, indicating no change in singlet oxygen (1O2) formation. The decreased ΦPSII after feeding was due to a decreased fraction of open reaction centers (qp), since the efficiency of open PSII reaction centers to utilize the light energy (Fv′/Fm′) did not differ before and after feeding. The decreased fraction of open reaction centers resulted in increased excess excitation energy (EXC) at the feeding zone and at the whole leaf level, while hydrogen peroxide (H2O2) production was detected only at the feeding zone. Although the whole leaf PSII efficiency decreased compared to that before feeding, the maximum efficiency of PSII photochemistry (Fv/Fm), and the efficiency of the water-splitting complex on the donor side of PSII (Fv/Fo), did not differ to that before feeding, thus they cannot be considered as sensitive parameters to monitor biotic stress effects. Chlorophyll fluorescence imaging analysis proved to be a good indicator to monitor even short-term impacts of insect herbivory on photosynthetic function, and among the studied parameters, the reduction status of the plastoquinone pool (qp) was the most sensitive and suitable indicator to probe photosynthetic function under biotic stress.

ACS Style

Ilektra Sperdouli; Stefanos Andreadis; Julietta Moustaka; Emmanuel Panteris; Aphrodite Tsaballa; Michael Moustakas. Changes in Light Energy Utilization in Photosystem II and Reactive Oxygen Species Generation in Potato Leaves by the Pinworm Tuta absoluta. Molecules 2021, 26, 2984 .

AMA Style

Ilektra Sperdouli, Stefanos Andreadis, Julietta Moustaka, Emmanuel Panteris, Aphrodite Tsaballa, Michael Moustakas. Changes in Light Energy Utilization in Photosystem II and Reactive Oxygen Species Generation in Potato Leaves by the Pinworm Tuta absoluta. Molecules. 2021; 26 (10):2984.

Chicago/Turabian Style

Ilektra Sperdouli; Stefanos Andreadis; Julietta Moustaka; Emmanuel Panteris; Aphrodite Tsaballa; Michael Moustakas. 2021. "Changes in Light Energy Utilization in Photosystem II and Reactive Oxygen Species Generation in Potato Leaves by the Pinworm Tuta absoluta." Molecules 26, no. 10: 2984.

Journal article
Published: 30 January 2021 in International Journal of Molecular Sciences
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Cytokinesis is accomplished in higher plants by the phragmoplast, creating and conducting the cell plate to separate daughter nuclei by a new cell wall. The microtubule-severing enzyme p60-katanin plays an important role in the centrifugal expansion and timely disappearance of phragmoplast microtubules. Consequently, aberrant structure and delayed expansion rate of the phragmoplast have been reported to occur in p60-katanin mutants. Here, the consequences of p60-katanin malfunction in cell plate/daughter wall formation were investigated by transmission electron microscopy (TEM), in root cells of the fra2 Arabidopsis thaliana loss-of-function mutant. In addition, deviations in the chemical composition of cell plate/new cell wall were identified by immunolabeling and confocal microscopy. It was found that, apart from defective phragmoplast microtubule organization, cell plates/new cell walls also appeared faulty in structure, being unevenly thick and perforated by large gaps. In addition, demethylesterified homogalacturonans were prematurely present in fra2 cell plates, while callose content was significantly lower than in the wild type. Furthermore, KNOLLE syntaxin disappeared from newly formed cell walls in fra2 earlier than in the wild type. Taken together, these observations indicate that delayed cytokinesis, due to faulty phragmoplast organization and expansion, results in a loss of synchronization between cell plate growth and its chemical maturation.

ACS Style

Emmanuel Panteris; Anna Kouskouveli; Dimitris Pappas; Ioannis-Dimosthenis Adamakis. Cytokinesis in fra2 Arabidopsis thaliana p60-katanin Mutant: Defects in Cell Plate/Daughter Wall Formation. International Journal of Molecular Sciences 2021, 22, 1405 .

AMA Style

Emmanuel Panteris, Anna Kouskouveli, Dimitris Pappas, Ioannis-Dimosthenis Adamakis. Cytokinesis in fra2 Arabidopsis thaliana p60-katanin Mutant: Defects in Cell Plate/Daughter Wall Formation. International Journal of Molecular Sciences. 2021; 22 (3):1405.

Chicago/Turabian Style

Emmanuel Panteris; Anna Kouskouveli; Dimitris Pappas; Ioannis-Dimosthenis Adamakis. 2021. "Cytokinesis in fra2 Arabidopsis thaliana p60-katanin Mutant: Defects in Cell Plate/Daughter Wall Formation." International Journal of Molecular Sciences 22, no. 3: 1405.

Preprint
Published: 12 January 2021
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Cytokinesis is accomplished in higher plants by the phragmoplast, creating and conducting the cell plate, to separate daughter nuclei by a new cell wall. The microtubule-severing enzyme p60-katanin plays an important role in the centrifugal expansion and timely disappearance of phragmoplast microtubules. Consequently, aberrant structure and delayed expansion rate of the phragmoplast occur in p60-katanin mutants. Here, the consequences of p60-katanin malfunction in cell plate/daughter wall formation were investigated by transmission electron microscopy (TEM), while deviations in the chemical composition of cell plate/new cell wall were identified by immunolabeling and confocal microscopy, in root cells of the fra2 Arabidopsis thaliana mutant. It was found that, apart from defective phragmoplast microtubule organization, cell plates/new cell walls appeared also faulty in structure, being unevenly thick and perforated by large gaps. In addition, demethylesterified homogalacturonans were prematurely present in fra2 cell plates, while callose content was significantly lower than in the wild-type. Furthermore, KNOLLE syntaxin disappeared from newly formed cell walls in fra2 earlier than in the wild-type. Taken together, these observations indicate that delayed cytokinesis, due to faulty phragmoplast organization and expansion, results in a loss of synchronization between cell plate growth and its chemical maturation.

ACS Style

Emmanuel Panteris; Anna Kouskouveli; Dimitris Pappas; Ioannis-Dimosthenis S. Adamakis. Cytokinesis in fra2 Arabidopsis thaliana p60-katanin Mutant: Defects in Cell Plate/Daughter Wall Formation. 2021, 1 .

AMA Style

Emmanuel Panteris, Anna Kouskouveli, Dimitris Pappas, Ioannis-Dimosthenis S. Adamakis. Cytokinesis in fra2 Arabidopsis thaliana p60-katanin Mutant: Defects in Cell Plate/Daughter Wall Formation. . 2021; ():1.

Chicago/Turabian Style

Emmanuel Panteris; Anna Kouskouveli; Dimitris Pappas; Ioannis-Dimosthenis S. Adamakis. 2021. "Cytokinesis in fra2 Arabidopsis thaliana p60-katanin Mutant: Defects in Cell Plate/Daughter Wall Formation." , no. : 1.

Journal article
Published: 17 December 2020 in International Journal of Molecular Sciences
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Microcystins (MCs) are cyanobacterial toxins and potent inhibitors of protein phosphatases 1 (PP1) and 2A (PP2A), which are involved in plant cytoskeleton (microtubules and F-actin) organization. Therefore, studies on the toxicity of cyanobacterial products on plant cells have so far been focused on MCs. In this study, we investigated the effects of extracts from 16 (4 MC-producing and 12 non-MC-producing) cyanobacterial strains from several habitats, on various enzymes (PP1, trypsin, elastase), on the plant cytoskeleton and H2O2 levels in Oryza sativa (rice) root cells. Seedling roots were treated for various time periods (1, 12, and 24 h) with aqueous cyanobacterial extracts and underwent either immunostaining for α-tubulin or staining of F-actin with fluorescent phalloidin. 2,7-dichlorofluorescein diacetate (DCF-DA) staining was performed for H2O2 imaging. The enzyme assays confirmed the bioactivity of the extracts of not only MC-rich (MC+), but also MC-devoid (MC−) extracts, which induced major time-dependent alterations on both components of the plant cytoskeleton. These findings suggest that a broad spectrum of bioactive cyanobacterial compounds, apart from MCs or other known cyanotoxins (such as cylindrospermopsin), can affect plants by disrupting the cytoskeleton.

ACS Style

Dimitris Pappas; Manthos Panou; Ioannis-Dimosthenis S. Adamakis; Spyros Gkelis; Emmanuel Panteris. Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells. International Journal of Molecular Sciences 2020, 21, 9649 .

AMA Style

Dimitris Pappas, Manthos Panou, Ioannis-Dimosthenis S. Adamakis, Spyros Gkelis, Emmanuel Panteris. Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells. International Journal of Molecular Sciences. 2020; 21 (24):9649.

Chicago/Turabian Style

Dimitris Pappas; Manthos Panou; Ioannis-Dimosthenis S. Adamakis; Spyros Gkelis; Emmanuel Panteris. 2020. "Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells." International Journal of Molecular Sciences 21, no. 24: 9649.

Preprint
Published: 26 November 2020
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Microcystins (MCs) are cyanobacterial toxins and potent inhibitors of protein phosphatases 1 (PP1) and 2A (PP2A), which are involved in plant cytoskeleton (microtubules and F-actin) organization. Therefore, studies on the toxicity of cyanobacterial products on plant cells have so far being focused on MCs. In this study, we investigated the effects of extracts from 16 (4 MC-producing and 12 non-MC-producing) cyanobacterial strains from several habitats, on various enzymes (PP1, trypsin, elastase), on the plant cytoskeleton and H2O2 levels in Oryza sativa (rice) root cells. Seedling roots were treated for various time periods (1, 12 and 24h) with aqueous cyanobacterial extracts and underwent either immunostaining for α-tubulin or staining of F-actin with fluorescent phalloidin. DCF-DA staining was performed for H2O2 imaging. The enzyme assays confirmed the bioactivity of the extracts of not only MC-rich (MC+), but also MC-devoid (MC-) extracts, which induced major time-dependent alterations on both components of the plant cytoskeleton. These findings suggest that a broad spectrum of bioactive cyanobacterial compounds, apart from MCs or other known cyanotoxins (such as cylindrospermopsin), can affect plants by disrupting the cytoskeleton.

ACS Style

Dimitris Pappas; Manthos Panou; Ioannis-Dimosthenis S. Adamakis; Spyros Gkelis; Emmanuel Panteris. Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells. 2020, 1 .

AMA Style

Dimitris Pappas, Manthos Panou, Ioannis-Dimosthenis S. Adamakis, Spyros Gkelis, Emmanuel Panteris. Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells. . 2020; ():1.

Chicago/Turabian Style

Dimitris Pappas; Manthos Panou; Ioannis-Dimosthenis S. Adamakis; Spyros Gkelis; Emmanuel Panteris. 2020. "Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells." , no. : 1.

Journal article
Published: 18 September 2020 in Journal of Hazardous Materials
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The organic pollutant bisphenol A (BPA) causes adverse effects on aquatic biota. The present study explored the toxicity mechanism of environmentally occurring BPA concentrations (0.03-3 μg L−1) on the seagrass Cymodocea nodosa intermediate leaf photosynthetic machinery. A "mosaic" type BPA effect pattern was observed, with “unaffected” and “affected” leaf areas. In negatively affected leaf areas cells had a dark appearance and lost their chlorophyll auto-fluorescence, while hydrogen peroxide (H2O2) content increased time-dependently. In the “unaffected” leaf areas, cells exhibited increased phenolic compound production. At 1 μg L-1 of BPA exposure, there was no effect on the fraction of open reaction centers (qP) compared to control and also no significant effect on the quantum yield of non-regulated non-photochemical energy loss in PSII (ΦΝΟ). However, a 3 μg L−1 BPA application resulted in a significant ΦΝΟ increase, even from the first exposure day. Ultrastructural observations revealed electronically dense damaged thylakoids in the plastids, while effects on Golgi dictyosomes and the endoplasmic reticulum were also observed at 3 μg L−1 BPA. The up-regulated H2O2 BPA-derived production seems to be a key factor causing both oxidative damages but probably also triggering retrograde signalling, conferring tolerance to BPA in the “unaffected” leaf areas.

ACS Style

Ioannis-Dimosthenis S. Adamakis; Paraskevi Malea; Ilektra Sperdouli; Emmanuel Panteris; Danae Kokkinidi; Michael Moustakas. Evaluation of the spatiotemporal effects of bisphenol A on the leaves of the seagrass Cymodocea nodosa. Journal of Hazardous Materials 2020, 404, 124001 .

AMA Style

Ioannis-Dimosthenis S. Adamakis, Paraskevi Malea, Ilektra Sperdouli, Emmanuel Panteris, Danae Kokkinidi, Michael Moustakas. Evaluation of the spatiotemporal effects of bisphenol A on the leaves of the seagrass Cymodocea nodosa. Journal of Hazardous Materials. 2020; 404 ():124001.

Chicago/Turabian Style

Ioannis-Dimosthenis S. Adamakis; Paraskevi Malea; Ilektra Sperdouli; Emmanuel Panteris; Danae Kokkinidi; Michael Moustakas. 2020. "Evaluation of the spatiotemporal effects of bisphenol A on the leaves of the seagrass Cymodocea nodosa." Journal of Hazardous Materials 404, no. : 124001.

Journal article
Published: 25 July 2020 in Pharmaceutics
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Amphotericin B (AmpB), one of the most commonly used agents in the treatment of severe fungal infections and life-threatening parasitic diseases such as visceral Leishmaniasis, has a negligible oral bioavailability, primarily due to a low solubility and permeability. To develop an oral formulation, medium chain triglycerides and nonionic surfactants in a self-nano-emulsifying drug delivery system (SNEDDS) containing AmpB were combined with room temperature ionic liquids (RTILs) of imidazolium. The presence of ionic liquids significantly enhanced the solubility of AmpB, exhibited a low toxicity and increased the transport of AmpB across Caco-2 cell monolayers. The combination of RTILs with a lipid formulation might be a promising strategy to improve the oral bioavailability of AmpB.

ACS Style

Eleni Kontogiannidou; Thomas Meikopoulos; Helen Gika; Emmanuel Panteris; Ioannis S. Vizirianakis; Anette Müllertz; Dimitrios G. Fatouros. In Vitro Evaluation of Self-Nano-Emulsifying Drug Delivery Systems (SNEDDS) Containing Room Temperature Ionic Liquids (RTILs) for the Oral Delivery of Amphotericin B. Pharmaceutics 2020, 12, 699 .

AMA Style

Eleni Kontogiannidou, Thomas Meikopoulos, Helen Gika, Emmanuel Panteris, Ioannis S. Vizirianakis, Anette Müllertz, Dimitrios G. Fatouros. In Vitro Evaluation of Self-Nano-Emulsifying Drug Delivery Systems (SNEDDS) Containing Room Temperature Ionic Liquids (RTILs) for the Oral Delivery of Amphotericin B. Pharmaceutics. 2020; 12 (8):699.

Chicago/Turabian Style

Eleni Kontogiannidou; Thomas Meikopoulos; Helen Gika; Emmanuel Panteris; Ioannis S. Vizirianakis; Anette Müllertz; Dimitrios G. Fatouros. 2020. "In Vitro Evaluation of Self-Nano-Emulsifying Drug Delivery Systems (SNEDDS) Containing Room Temperature Ionic Liquids (RTILs) for the Oral Delivery of Amphotericin B." Pharmaceutics 12, no. 8: 699.

Journal article
Published: 24 July 2020 in Cancers
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Downregulation of the cylindromatosis (CYLD) tumor suppressor has been associated with breast cancer development and progression. Here, we report a critical role for CYLD in maintaining the phenotype of mammary epithelial cells in vitro and in vivo. CYLD downregulation or inactivation induced an epithelial to mesenchymal transition of mammary epithelial cells that was dependent on the concomitant activation of the transcription factors Yes-associated protein (YAP)/transcriptional coactivator with PDZ-binding motif (TAZ) and transforming growth factor beta (TGF)signaling. CYLD inactivation enhanced the nuclear localization of YAP/TAZ and the phosphorylation of Small Mothers Against Decapentaplegic (SMAD)2/3 proteins in confluent cell culture conditions. Consistent with these findings were the hyperplastic alterations of CYLD-deficient mouse mammary epithelia, which were associated with enhanced nuclear expression of the YAP/TAZ transcription factors. Furthermore, in human breast cancer samples, downregulation of CYLD expression correlates with enhanced YAP/TAZ-regulated target gene expression. Our results identify CYLD as a critical regulator of a signaling node that prevents the coordinated activation of YAP/TAZ and the TGF pathway in mammary epithelial cells, in order to maintain their phenotypic identity and homeostasis. Consequently, they provide a novel conceptual framework that supports and explains a causal implication of deficient CYLD expression in aggressive human breast cancers.

ACS Style

Athanasios Pseftogas; Konstantinos Xanthopoulos; Theofilos Poutahidis; Chrysanthi Ainali; Dimitra Dafou; Emmanuel Panteris; Joseph G. Kern; Xaralabos Varelas; Alexander Hardas; Christos Gonidas; Anastasia Tsingotjidou; Eudoxia Hatzivassiliou; George Mosialos. The Tumor Suppressor CYLD Inhibits Mammary Epithelial to Mesenchymal Transition by the Coordinated Inhibition of YAP/TAZ and TGF Signaling. Cancers 2020, 12, 2047 .

AMA Style

Athanasios Pseftogas, Konstantinos Xanthopoulos, Theofilos Poutahidis, Chrysanthi Ainali, Dimitra Dafou, Emmanuel Panteris, Joseph G. Kern, Xaralabos Varelas, Alexander Hardas, Christos Gonidas, Anastasia Tsingotjidou, Eudoxia Hatzivassiliou, George Mosialos. The Tumor Suppressor CYLD Inhibits Mammary Epithelial to Mesenchymal Transition by the Coordinated Inhibition of YAP/TAZ and TGF Signaling. Cancers. 2020; 12 (8):2047.

Chicago/Turabian Style

Athanasios Pseftogas; Konstantinos Xanthopoulos; Theofilos Poutahidis; Chrysanthi Ainali; Dimitra Dafou; Emmanuel Panteris; Joseph G. Kern; Xaralabos Varelas; Alexander Hardas; Christos Gonidas; Anastasia Tsingotjidou; Eudoxia Hatzivassiliou; George Mosialos. 2020. "The Tumor Suppressor CYLD Inhibits Mammary Epithelial to Mesenchymal Transition by the Coordinated Inhibition of YAP/TAZ and TGF Signaling." Cancers 12, no. 8: 2047.

Research paper
Published: 08 April 2020 in Plant Biology
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Microcystins are toxins produced by cyanobacteria, notorious for negatively affecting a wide range of living organisms, among which several plant species. Although microtubules are a well‐established target of microcystin toxicity, its effect on filamentous actin (F‐actin) in plant cells has not been studied yet. Τhe effects of microcystin‐LR (MC‐LR) and the extract of a microcystin‐producing freshwater cyanobacterial strain (Microcystis flos‐aquae TAU‐MAC 1510) on the cytoskeleton (F‐actin and microtubules) of Oryza sativa (rice) root cells, were studied by light, confocal, and transmission electron microscopy. Considering the role of F‐actin in endomembrane system distribution, the endoplasmic reticulum and the Golgi apparatus in extract‐treated cells were also examined. F‐actin in both MC‐LR‐ and extract‐treated meristematic and differentiating root cells exhibited time‐dependent alterations, ranging from disorientation and bundling to the formation of ring‐like structures, eventually resulting to a collapse of the F‐actin network at longer treatments. Disorganization and eventual depolymerization of microtubules, as well as abnormal chromatin condensation were observed following treatment with the extract, effects which could be attributed to microcystins and other bioactive compounds. Moreover, cell cycle progression was inhibited in extract‐treated roots, specifically affecting the mitotic events. As a consequence of F‐actin network disorganization, endoplasmic reticulum elements appeared stacked and diminished, while Golgi dictyosomes appeared aggregated. These results support that F‐actin is a prominent target of MC‐LR, both in pure form and as an extract ingredient. Endomembrane system alterations can also be attributed to the effects of cyanobacterial bioactive compounds (including microcystins) on F‐actin cytoskeleton.

ACS Style

D. Pappas; S. Gkelis; E. Panteris. The effects of microcystin‐LR in Oryza sativa root cells: F‐actin as a new target of cyanobacterial toxicity. Plant Biology 2020, 22, 839 -849.

AMA Style

D. Pappas, S. Gkelis, E. Panteris. The effects of microcystin‐LR in Oryza sativa root cells: F‐actin as a new target of cyanobacterial toxicity. Plant Biology. 2020; 22 (5):839-849.

Chicago/Turabian Style

D. Pappas; S. Gkelis; E. Panteris. 2020. "The effects of microcystin‐LR in Oryza sativa root cells: F‐actin as a new target of cyanobacterial toxicity." Plant Biology 22, no. 5: 839-849.

Preprint content
Published: 18 February 2020
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Microcystins are toxins produced by cyanobacteria, notorious for negatively affecting a wide range of living organisms, among which several plant species. Although microtubules are a well-established target of microcystin toxicity, its effect on filamentous actin (F-actin) in plant cells has not been studied yet.The effects of microcystin-LR (MC-LR) and the extract of a microcystin-producing freshwater cyanobacterial strain (Microcystis flos-aquae TAU-MAC 1510) on the cytoskeleton (F-actin and microtubules) of Oryza sativa (rice) root cells, were studied by light, confocal, and transmission electron microscopy. Considering the role of F-actin in endomembrane system distribution, the endoplasmic reticulum and the Golgi apparatus in extract-treated cells were also examined.F-actin in both MC-LR- and extract-treated meristematic and differentiating root cells exhibited time-dependent alterations, ranging from disorientation and bundling to the formation of ring-like structures, eventually resulting to a collapse of the F-actin network at longer treatments. Disorganization and eventual depolymerization of microtubules, as well as abnormal chromatin condensation were observed following treatment with the extract, effects which could be attributed to microcystins and other bioactive compounds. Moreover, cell cycle progression was inhibited in extract-treated roots, specifically affecting the mitotic events. As a consequence of F-actin network disorganization, endoplasmic reticulum elements appeared stacked and diminished, while Golgi dictyosomes appeared aggregated.These results support that F-actin is a prominent target of MC-LR, both in pure form and as an extract ingredient. Endomembrane system alterations can also be attributed to the effects of cyanobacterial bioactive compounds (including microcystins) on F-actin cytoskeleton.

ACS Style

Dimitris Pappas; Spyros Gkelis; Emmanuel Panteris. The effects of microcystin-LR in Oryza sativa root cells: F-actin as a new target of cyanobacterial toxicity. 2020, 1 .

AMA Style

Dimitris Pappas, Spyros Gkelis, Emmanuel Panteris. The effects of microcystin-LR in Oryza sativa root cells: F-actin as a new target of cyanobacterial toxicity. . 2020; ():1.

Chicago/Turabian Style

Dimitris Pappas; Spyros Gkelis; Emmanuel Panteris. 2020. "The effects of microcystin-LR in Oryza sativa root cells: F-actin as a new target of cyanobacterial toxicity." , no. : 1.

Journal article
Published: 11 May 2019 in Biomolecules
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The effects of bisphenol A (BPA), a prevalent endocrine disruptor, on both interphase and mitotic microtubule array organization was examined by immunofluorescence microscopy in meristematic root cells of Triticum turgidum (durum wheat) and Allium cepa (onion). In interphase cells of A. cepa, BPA treatment resulted in substitution of cortical microtubules by annular/spiral tubulin structures, while in T. turgidum BPA induced cortical microtubule fragmentation. Immunolocalization of acetylated α-tubulin revealed that cortical microtubules of T. turgidum were highly acetylated, unlike those of A. cepa. In addition, elevation of tubulin acetylation by trichostatin A in A. cepa resulted in microtubule disruption similar to that observed in T. turgidum. BPA also disrupted all mitotic microtubule arrays in both species. It is also worth noting that mitotic microtubule arrays were acetylated in both plants. As assessed by BPA removal, its effects are reversible. Furthermore, taxol-stabilized microtubules were resistant to BPA, while recovery from oryzalin treatment in BPA solution resulted in the formation of ring-like tubulin conformations. Overall, these findings indicate the following: (1) BPA affects plant mitosis/cytokinesis by disrupting microtubule organization. (2) Microtubule disassembly probably results from impairment of free tubulin subunit polymerization. (3) The differences in cortical microtubule responses to BPA among the species studied are correlated to the degree of tubulin acetylation.

ACS Style

Ioannis-Dimosthenis S. Adamakis; Emmanuel Panteris; Eleftherios P. Eleftheriou. Tubulin Acetylation Mediates Bisphenol A Effects on the Microtubule Arrays of Allium cepa and Triticum turgidum. Biomolecules 2019, 9, 185 .

AMA Style

Ioannis-Dimosthenis S. Adamakis, Emmanuel Panteris, Eleftherios P. Eleftheriou. Tubulin Acetylation Mediates Bisphenol A Effects on the Microtubule Arrays of Allium cepa and Triticum turgidum. Biomolecules. 2019; 9 (5):185.

Chicago/Turabian Style

Ioannis-Dimosthenis S. Adamakis; Emmanuel Panteris; Eleftherios P. Eleftheriou. 2019. "Tubulin Acetylation Mediates Bisphenol A Effects on the Microtubule Arrays of Allium cepa and Triticum turgidum." Biomolecules 9, no. 5: 185.

Research article
Published: 26 April 2019 in Molecular Pharmaceutics
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Combination therapy has been conferred with manifold assets leveraging the synergy of different agents to achieve sufficient therapeutic outcome with lower administered drug doses and reduced side effects. The therapeutic potency of a self-assembling peptide hydrogel for the co-delivery of doxorubicin (DOX) and curcumin (CUR) combination was evaluated against head and neck cancer cells. The dual loaded peptide hydrogel enabled control over the rate of drug release based on drug’s aqueous solubility. A significantly enhanced cell growth inhibitory effect was observed after treatment with the combination drug loaded hydrogel formulations compared to the respective combination drug solution. The synergistic pharmacological effect of selected hydrogel formulations was further confirmed with enhanced apoptotic cell response, interference in cell cycle progression and significantly altered apoptotic/anti-apoptotic gene expression profiles obtained in dose levels well below the half-maximal inhibitory concentrations of both drugs. The in vivo antitumour efficacy of the drug loaded peptide hydrogel formulation was confirmed in HSC-3 cell-xenografted SCID mice and visualized with μCT imaging. Histological and TUNEL assay analyses of major organs were implemented to assess the safety of the topically administered hydrogel formulation. Overall, results demonstrated the therapeutic utility of the dual drug-loaded peptide hydrogel as a promising approach for the local treatment of head and neck cancer.

ACS Style

Christina Karavasili; Dimitrios A. Andreadis; Orestis L. Katsamenis; Emmanuel Panteris; Pinelopi Anastasiadou; Zacharias Kakazanis; Vasilis Zoumpourlis; Catherine K. Markopoulou; Sotirios Koutsopoulos; Ioannis S. Vizirianakis; Dimitrios G. Fatouros. Synergistic Antitumor Potency of a Self-Assembling Peptide Hydrogel for the Local Co-delivery of Doxorubicin and Curcumin in the Treatment of Head and Neck Cancer. Molecular Pharmaceutics 2019, 16, 2326 -2341.

AMA Style

Christina Karavasili, Dimitrios A. Andreadis, Orestis L. Katsamenis, Emmanuel Panteris, Pinelopi Anastasiadou, Zacharias Kakazanis, Vasilis Zoumpourlis, Catherine K. Markopoulou, Sotirios Koutsopoulos, Ioannis S. Vizirianakis, Dimitrios G. Fatouros. Synergistic Antitumor Potency of a Self-Assembling Peptide Hydrogel for the Local Co-delivery of Doxorubicin and Curcumin in the Treatment of Head and Neck Cancer. Molecular Pharmaceutics. 2019; 16 (6):2326-2341.

Chicago/Turabian Style

Christina Karavasili; Dimitrios A. Andreadis; Orestis L. Katsamenis; Emmanuel Panteris; Pinelopi Anastasiadou; Zacharias Kakazanis; Vasilis Zoumpourlis; Catherine K. Markopoulou; Sotirios Koutsopoulos; Ioannis S. Vizirianakis; Dimitrios G. Fatouros. 2019. "Synergistic Antitumor Potency of a Self-Assembling Peptide Hydrogel for the Local Co-delivery of Doxorubicin and Curcumin in the Treatment of Head and Neck Cancer." Molecular Pharmaceutics 16, no. 6: 2326-2341.

Regular article
Published: 18 April 2019 in Journal of Phycology
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Cyanobacterial diversity associated with sponges remains underestimated, though it is of great scientific interest in order to understand the ecology and evolutionary history of the symbiotic relationships between the two groups. Of the filamentous cyanobacteria, the genus Leptolyngbya is the most frequently found in association with sponges as well as the largest and obviously polyphyletic group. In this study, five Leptolyngbya‐like sponge‐associated isolates were investigated using a combination of molecular, chemical, and morphological approach and revealed a novel marine genus herein designated Leptothoe gen. nov. In addition, three new species of Leptothoe, Le. sithoniana, Le. kymatousa and Le. spongobia, are described based on a suite of distinct characters compared to other marine Leptolyngbyaceae species/strains. The three new species, hosted by four sponge species, showed different degrees of host specificity. Leptothoe sithoniana and Le. kymatousa hosted by the sponges Petrosia ficiformis and Chondrilla nucula respectively, seem to be more specialized than Le. spongobia, which was hosted by the sponges Dysidea avara and Acanthella acuta. All three species contained nitrogen‐fixing genes and may contribute to the nitrogen budget of sponges. Leptothoe spongobia TAU‐MAC 1115 isolated from Acanthella acuta, was shown to produce microcystin‐RR, indicating that microcystin production among marine cyanobacteria could be more widespread than previously determined.

ACS Style

Despoina Konstantinou; Eleni Voultsiadou; Emmanuel Panteris; Sevasti‐Kiriaki Zervou; Anastasia Hiskia; Spyros Gkelis. Leptothoe, a new genus of marine cyanobacteria (Synechococcales) and three new species associated with sponges from the Aegean Sea. Journal of Phycology 2019, 55, 882 -897.

AMA Style

Despoina Konstantinou, Eleni Voultsiadou, Emmanuel Panteris, Sevasti‐Kiriaki Zervou, Anastasia Hiskia, Spyros Gkelis. Leptothoe, a new genus of marine cyanobacteria (Synechococcales) and three new species associated with sponges from the Aegean Sea. Journal of Phycology. 2019; 55 (4):882-897.

Chicago/Turabian Style

Despoina Konstantinou; Eleni Voultsiadou; Emmanuel Panteris; Sevasti‐Kiriaki Zervou; Anastasia Hiskia; Spyros Gkelis. 2019. "Leptothoe, a new genus of marine cyanobacteria (Synechococcales) and three new species associated with sponges from the Aegean Sea." Journal of Phycology 55, no. 4: 882-897.

Journal article
Published: 01 August 2018 in Ecotoxicology and Environmental Safety
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Bisphenol A (BPA) is an emerging pollutant of environmental concern, classified as "moderately toxic" and "toxic", causing adverse effects on aquatic biota. Although information about BPA toxicity on aquatic fauna is available, the data about BPA effects on aquatic flora remain scarce, missing for marine macrophytes. The effects of environmentally relevant BPA concentrations (ranging from 0.03 to 3 μg L) on juvenile leaf elongation and the cytoskeleton (microtubules, MTs and actin filaments, AFs) were studied in the seagrass Cymodocea nodosa for 1-10 days. The suitability of cytoskeleton disturbance and leaf elongation impairment as "biomarkers" for BPA stress were tested. The highest BPA concentrations (0.3, 0.5, 1 and 3 μg L) affected significantly leaf elongation from the onset of the experiment, while defects of the cytoskeleton were observed even at lower concentrations. In particular, MTs were initially disrupted (i.e. "lowest observed effect concentrations", LOECs) at 0.1 μg L, while AFs were damaged even at 0.03 μg L. AFs appeared thus to be more sensitive to lower BPA concentrations, while there was a correlation between leaf elongation impairment and MT defects. Thus, AF damages, MT disruption and leaf elongation impairment in C. nodosa, in this particular order, appear to be sensitive "biomarkers" of BPA stress, at the above environmentally relevant BPA concentrations.

ACS Style

Ioannis-Dimosthenis S. Adamakis; Paraskevi Malea; Emmanuel Panteris. The effects of Bisphenol A on the seagrass Cymodocea nodosa: Leaf elongation impairment and cytoskeleton disturbance. Ecotoxicology and Environmental Safety 2018, 157, 431 -440.

AMA Style

Ioannis-Dimosthenis S. Adamakis, Paraskevi Malea, Emmanuel Panteris. The effects of Bisphenol A on the seagrass Cymodocea nodosa: Leaf elongation impairment and cytoskeleton disturbance. Ecotoxicology and Environmental Safety. 2018; 157 ():431-440.

Chicago/Turabian Style

Ioannis-Dimosthenis S. Adamakis; Paraskevi Malea; Emmanuel Panteris. 2018. "The effects of Bisphenol A on the seagrass Cymodocea nodosa: Leaf elongation impairment and cytoskeleton disturbance." Ecotoxicology and Environmental Safety 157, no. : 431-440.

Research paper
Published: 25 June 2018 in Pharmaceutical Research
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Localized chemotherapy has gained significant impetus for the management of malignant brain tumors. In the present study, we appraised the versatility of an in-situ gel forming self-assembling peptide, ac-(RADA)4-CONH2, as a biocompatible delivery depot of the chemotherapeutic drug doxorubicin (DOX) and the anticancer agent curcumin (CUR), respectively. The morphology and mechanical properties of ac-(RADA)4-CONH2 were assessed with scanning electron microscopy (SEM) and rheological studies. The in vitro drug release from ac-(RADA)4-CONH2 was monitored in phosphate-buffered saline pH 7.4. Distribution of the fluorescent actives within the peptide matrix was visualized with confocal laser scanning microscopy (CLSM). The in vitro biological performance of the ac-(RADA)4-CONH2-DOX and ac-(RADA)4-CONH2-CUR was evaluated on the human glioblastoma U-87 MG cell line. SEM studies revealed that the ac-(RADA)4-CONH2 hydrogel contains an entangled nanofiber network. Rheology studies showed that the more hydrophobic CUR resulted in a stiffer hydrogel compared with ac-(RADA)4-CONH2 and ac-(RADA)4-CONH2-DOX, due to the interaction of CUR with the hydrophobic domains of the peptide nanofibers as confirmed by CLSM. In vitro release studies showed a complete DOX release from ac-(RADA)4-CONH2 within 4 days and a prolonged release for ac-(RADA)4-CONH2-CUR over 20 days. An increased cellular uptake and a higher cytotoxic effect were observed for ac-(RADA)4-CONH2-DOX, compared with DOX solution. Higher levels of early apoptosis were observed for the cells treated with the ac-(RADA)4-CONH2-CUR, compared to CUR solution. The current findings highlight the potential utility of the in-situ depot forming ac-(RADA)4-CONH2 hydrogel for the local delivery of both water soluble and insoluble chemotherapeutic drugs.

ACS Style

Christina Karavasili; Emmanuel Panteris; Ioannis Vizirianakis; Sotirios Koutsopoulos; Dimitrios G. Fatouros. Chemotherapeutic Delivery from a Self-Assembling Peptide Nanofiber Hydrogel for the Management of Glioblastoma. Pharmaceutical Research 2018, 35, 166 .

AMA Style

Christina Karavasili, Emmanuel Panteris, Ioannis Vizirianakis, Sotirios Koutsopoulos, Dimitrios G. Fatouros. Chemotherapeutic Delivery from a Self-Assembling Peptide Nanofiber Hydrogel for the Management of Glioblastoma. Pharmaceutical Research. 2018; 35 (8):166.

Chicago/Turabian Style

Christina Karavasili; Emmanuel Panteris; Ioannis Vizirianakis; Sotirios Koutsopoulos; Dimitrios G. Fatouros. 2018. "Chemotherapeutic Delivery from a Self-Assembling Peptide Nanofiber Hydrogel for the Management of Glioblastoma." Pharmaceutical Research 35, no. 8: 166.

Journal article
Published: 15 June 2018 in Journal of Biological Research-Thessaloniki
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Transverse cortical microtubule orientation, critical for anisotropic cell expansion, is established in the meristematic root zone. Intending to elucidate the possible prerequisites for this establishment and factors that are involved, microtubule organization was studied in roots of Arabidopsis thaliana, wild-type and the p60-katanin mutants fra2, ktn1-2 and lue1. Transverse cortical microtubule orientation in the meristematic root zone has proven to persist under several regimes inhibiting root elongation. This persistence was attributed to the constant moderate elongation of meristematic cells, prior to mitotic division. Therefore, A. thaliana wild-type seedlings were treated with aphidicolin, in order to prevent mitosis and inhibit premitotic cell elongation. In roots treated with aphidicolin for 12 h, cell divisions still occurred and microtubules were transverse. After 24 and 48 h of treatment, meristematic cell divisions and the prerequisite elongation ceased, while microtubule orientation became random. In meristematic cells of the p60-katanin mutants, apart from a general transverse microtubule pattern, cortical microtubules with random orientation were observed, also converging at several cortical sites, in contrast to the uniform transverse pattern of wild-type cells. Taken together, these observations reveal that transverse cortical microtubule orientation in the meristematic zone of A. thaliana root is cell division-dependent and requires severing by katanin.

ACS Style

Emmanuel Panteris; Barbara-Evelin Diannelidis; Ioannis-Dimosthenis S. Adamakis. Cortical microtubule orientation in Arabidopsis thaliana root meristematic zone depends on cell division and requires severing by katanin. Journal of Biological Research-Thessaloniki 2018, 25, 12 .

AMA Style

Emmanuel Panteris, Barbara-Evelin Diannelidis, Ioannis-Dimosthenis S. Adamakis. Cortical microtubule orientation in Arabidopsis thaliana root meristematic zone depends on cell division and requires severing by katanin. Journal of Biological Research-Thessaloniki. 2018; 25 (1):12.

Chicago/Turabian Style

Emmanuel Panteris; Barbara-Evelin Diannelidis; Ioannis-Dimosthenis S. Adamakis. 2018. "Cortical microtubule orientation in Arabidopsis thaliana root meristematic zone depends on cell division and requires severing by katanin." Journal of Biological Research-Thessaloniki 25, no. 1: 12.

Original research article
Published: 13 June 2018 in Frontiers in Plant Science
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Although cytoskeleton is a driving force for cell division and growth in higher plants, there is little evidence about its components in parasitic angiosperms. Microtubules and actin filaments in cells of shoot apical meristem and root-like structure of stem holoparasites European (C. europaea L.) and Eastern (C. monogyna Vahl.) dodders, as well as in prehaustorium, the specific organ adapted to parasitism, were visualized for the first time by immunolabeling and fluorescence microscopy. The significance of cytoskeletal elements during germination and prehaustorium formation was addressed by treatments with taxol, oryzalin, latrunculin B, cytochalasin B/D, jasplakinolide, and 2,3-butanedione monoxime. In shoot apical meristem many dividing cells were observed, in contrast to root-like structure, devoid of cell divisions. Cortical microtubules were oriented transversely and/or obliquely, while actin filaments were randomly distributed in cells of both organs. Furthermore, longitudinal cortical microtubules were present in digitate cells of prehaustorium, and transverse arrays were found in its file cells. Long and short random actin filaments were also observed in prehaustorium cells. Thus, it was shown that the cytoskeleton in dodder shoot cells is organized in a similar way to non-parasitic dicots, while cytoskeletal organization has some peculiarities in quickly senescing root-like structure and prehaustorium.

ACS Style

Peter Kaštier; Yuliya A. Krasylenko; Michaela Martinčová; Emmanuel Panteris; Jozef Šamaj; Alžbeta Blehová. Cytoskeleton in the Parasitic Plant Cuscuta During Germination and Prehaustorium Formation. Frontiers in Plant Science 2018, 9, 1 .

AMA Style

Peter Kaštier, Yuliya A. Krasylenko, Michaela Martinčová, Emmanuel Panteris, Jozef Šamaj, Alžbeta Blehová. Cytoskeleton in the Parasitic Plant Cuscuta During Germination and Prehaustorium Formation. Frontiers in Plant Science. 2018; 9 ():1.

Chicago/Turabian Style

Peter Kaštier; Yuliya A. Krasylenko; Michaela Martinčová; Emmanuel Panteris; Jozef Šamaj; Alžbeta Blehová. 2018. "Cytoskeleton in the Parasitic Plant Cuscuta During Germination and Prehaustorium Formation." Frontiers in Plant Science 9, no. : 1.

Journal article
Published: 06 June 2018 in Molecules
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Cellulose microfibrils reinforce the cell wall for morphogenesis in plants. Herein, we provide evidence on a series of defects regarding stomatal complex development and F-actin organization in Zea mays leaf epidermis, due to inhibition of cellulose synthesis. Formative cell divisions of stomatal complex ontogenesis were delayed or inhibited, resulting in lack of subsidiary cells and frequently in unicellular stomata, with an atypical stomatal pore. Guard cells failed to acquire a dumbbell shape, becoming rounded, while subsidiary cells, whenever present, exhibited aberrant morphogenesis. F-actin organization was also affected, since the stomatal complex-specific arrays were scarcely observed. At late developmental stages, the overall F-actin network was diminished in all epidermal cells, although thick actin bundles persisted. Taken together, stomatal complex development strongly depends on cell wall mechanical properties. Moreover, F-actin organization exhibits a tight relationship with the cell wall.

ACS Style

Emmanuel Panteris; Theonymphi Achlati; Gerasimos Daras; Stamatis Rigas. Stomatal Complex Development and F-Actin Organization in Maize Leaf Epidermis Depend on Cellulose Synthesis. Molecules 2018, 23, 1365 .

AMA Style

Emmanuel Panteris, Theonymphi Achlati, Gerasimos Daras, Stamatis Rigas. Stomatal Complex Development and F-Actin Organization in Maize Leaf Epidermis Depend on Cellulose Synthesis. Molecules. 2018; 23 (6):1365.

Chicago/Turabian Style

Emmanuel Panteris; Theonymphi Achlati; Gerasimos Daras; Stamatis Rigas. 2018. "Stomatal Complex Development and F-Actin Organization in Maize Leaf Epidermis Depend on Cellulose Synthesis." Molecules 23, no. 6: 1365.

Journal article
Published: 01 March 2018 in Chemosphere
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Bisphenol A (BPA) is a widespread environmental pollutant, reportedly harmful to living organisms. In plant cells, BPA was shown to disrupt microtubule (MT) arrays and perturb mitosis, but its effects on filamentous actin (F-actin) have not been explored. Here we studied the effects of BPA on actin filaments (AFs) in meristematic root tip and leaf cells of Zea mays, by fluorescent labeling and confocal microscopy. Considering the typical dynamic interaction between MTs and AFs, the effects on these two essential components of the plant cytoskeleton were correlated. It was found that BPA disorganized rapidly AFs in a concentration- and time-dependent manner. The fine filaments were first to be affected, followed by the subcortical bundles, resulting in rod- and ring-like conformations. The observed differences in sensitivity between protodermal and cortex cells were attributed to the deeper location of the latter. Depolymerization or stabilization of MTs by relevant drugs (oryzalin, taxol) revealed that AF susceptibility to BPA depends on MT integrity. Developing leaves required harder and longer treatment to be affected by BPA. Ontogenesis of stomatal complexes was highly disturbed, arrangement of AFs and MT arrays was disordered and accuracy of cell division sequence was deranged or completely arrested. The effect of BPA confirmed that subsidiary cell mother cell polarization is not mediated by F-actin patch neither of preprophase band organization. On the overall, it is concluded that AFs in plant cells constitute a subcellular target of BPA and their disruption depends on their crosstalk with MTs.

ACS Style

Konstantina Stavropoulou; Ioannis-Dimosthenis S. Adamakis; Emmanuel Panteris; Ermioni-Makedonia Arseni; Eleftherios P. Eleftheriou. Disruption of actin filaments in Zea mays by bisphenol A depends on their crosstalk with microtubules. Chemosphere 2018, 195, 653 -665.

AMA Style

Konstantina Stavropoulou, Ioannis-Dimosthenis S. Adamakis, Emmanuel Panteris, Ermioni-Makedonia Arseni, Eleftherios P. Eleftheriou. Disruption of actin filaments in Zea mays by bisphenol A depends on their crosstalk with microtubules. Chemosphere. 2018; 195 ():653-665.

Chicago/Turabian Style

Konstantina Stavropoulou; Ioannis-Dimosthenis S. Adamakis; Emmanuel Panteris; Ermioni-Makedonia Arseni; Eleftherios P. Eleftheriou. 2018. "Disruption of actin filaments in Zea mays by bisphenol A depends on their crosstalk with microtubules." Chemosphere 195, no. : 653-665.