Traditional ecotoxicology primarily investigates pollutant toxicity through physiological, biochemical, and single-molecular indicators. However, the limited data obtained through this approach constrain its application in the mechanistic analysis of pollutant toxicity. Omics technologies have emerged as a major research focus in recent years, enabling the comprehensive and accurate analysis of biomolecular-level changes. The integration of multi-omics technologies can holistically reveal the molecular toxicity mechanisms of pollutants, representing a primary emphasis in current toxicological research. This paper introduces the fundamental concepts of genomics, transcriptomics, proteomics, and metabolomics, while reviewing recent advancements in integrated omics approaches within aquatic toxicology. Furthermore, it provides a reference framework for the implementation of multi-omics strategies in ecotoxicological investigations. While multi-omics integration enables the unprecedented reconstruction of pollutant-induced molecular cascades and earlier biomarker discovery (notably through microbiome–metabolome linkages), its full potential requires experimental designs, machine learning-enhanced data integration, and validation against traditional toxicological endpoints within environmentally relevant models.

The chemical flame retardants represented by organophosphate esters (OPEs) are widely used and have a serious impact on the environment. In this study, we collected data on the exposure levels of ten OPEs in Chinese soils in recent years and performed an ecological risk assessment. The results showed that the levels of OPEs varied considerably throughout different regions of China, with high exposure levels in highly urbanized or industrialized areas such as Guangdong Province and Northeast China, where the mean value was >200 ng/g. The content of OPEs in the soil in industrial and commercial areas was significantly higher than in other regions, indicating that the concentration of OPEs in the soil is closely related to local economic development and the degree of industrialization. Meanwhile, the number of studies reporting on OPEs and their exposure concentrations have increased significantly since 2018. Through the ecological risk assessment, it was found that TCP, EHDPP and TEHP pose high ecological risks. Although some OPEs, such as TCIPP, have low ecological risk levels overall, their high exposure concentrations are still worthy of attention. This study details the general status of OPE contamination in Chinese soils, which can serve as a reference for ecological environmental supervision.