Air-polishing with low abrasiveness powders is fast arising as a valid and mini-invasive instrument for the management of biofilm colonizing dental implants. In general, the reported advantage is the efficient removal of plaque with respect to the titanium integrity. In the present study, we evaluated the in situ plaque removal and the preventive efficacy in forestalling further infection of an innovative erythritol/chlorhexidine air-polishing powder and compared it with sodium bicarbonate. Accordingly, two peri-implantitis-linked biofilm formers, strains Staphylococcus aureus and Aggregatibacter actinomycetemcomitans, were selected and used to infect titanium disks before and after the air-polishing treatment to test its ability in biofilm removal and re-colonization inhibition, respectively. Biofilm cell numbers and viability were assayed by colony-forming unit (CFU) count and metabolic-colorimetric (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (XTT) assay. Results demonstrated that air-polishing performed with either sodium bicarbonate or erythritol/chlorhexidine was effective in reducing bacteria biofilm viability and number on pre-infected specimens, thus showing a similar ability in counteracting existing infection in situ; on the other hand, when air-polished pre-treated disks were infected, only erythritol/chlorhexidine powder showed higher post-treatment biofilm re-growth inhibition. Finally, surface analysis via mechanical profilometry failed to show an increase in titanium roughness, regardless of the powder selected, thus excluding any possible surface damage due to the use of either sodium bicarbonate or erythritol/chlorhexidine.

Objectives: To evaluate the clinical efficacy in the short‐term resolution of gingivitis of a novel protocol involving full‐mouth erythritol powder air polishing followed by ultrasonic calculus removal.Methods: Forty‐one healthy patients completed the study. Following a split‐mouth design, quadrants 1–4 and 2–3 were randomly allocated to receive air polishing followed by ultrasonic calculus removal (A+US) or traditional full‐mouth ultrasonic debridement followed by polishing with a rubber cup and prophylactic paste (US+P). Bleeding on probing (BoP) and plaque index (PI) were collected at baseline and 2 and 4 weeks. Moreover, the residual plaque area (RPA), treatment time and patient comfort/satisfaction were evaluated at the end of the treatment.Results: Both treatments showed a significant reduction in BoP and PI. At 4 weeks, A+US seems to reach a statistically significant lower BoP (8.7% [6.9; 10.9] vs. 11.6%[9.3; 14.4], p < 0.0001) and PI (10.7% [8.9; 13.0] vs. 12.3% [10.2; 14.9], p = 0.033). Moreover, A+US treatment time lasted on average 9.2% less than US+P (p < 0.0001) and was the preferred treatment for a significantly higher number of patients (73.2% vs. 17.1%, p = 0.0001).Conclusion: The A+US protocol is suitable for the short‐term resolution of plaque‐induced gingivitis.

Objectives: To date, scarce evidence exists around the application of subgingival air-polishing during treatment of severe periodontitis. The aim of this study was to evaluate the effect on the health-related and periodontitis-related subgingival microbiome of air-polishing during non-surgical treatment of deep bleeding pockets in stage III–IV periodontitis patients. Materials and methods: Forty patients with stage III–IV periodontitis were selected, and pockets with probing depth (PD) 5–9 mm and bleeding on probing were selected as experimental sites. All patients underwent a full-mouth session of erythritol powder supragingival air-polishing and ultrasonic instrumentation. Test group received additional subgingival air-polishing at experimental sites. Subgingival microbial samples were taken from the maxillary experimental site showing the deepest PD at baseline. Primary outcome of the first part of the present study was the 3-month change in the number of experimental sites. Additional analysis of periodontal pathogens and other sub-gingival plaque bacteria sampled at one experimental site at baseline and 3 months following treatment was performed through a real-time quantitative PCR microarray. Results: In the test group, a statistical increase of some health-related species was observed (Abiotropha defectiva, Capnocytophaga sputigena, and Lautropia mirabilis), together with the decrease of pathogens such as of Actinomyces israelii, Catonella morbi, Filifactor alocis, Porphyromonas endodontalis, Sele-nomonas sputigena, Tannerella forsythia, Treponema denticola, and Treponema socranskii. In the control group, statistical significance was found only in the decrease of Filifactor alocis, Tannerella forsythia, and Treponema socranskii. Conclusions: The addition of erythritol-chlorhexidine powder seems to cause a shift of the periodontal micro-biome toward a more eubiotic condition compared to a conventional treatment. The study was registered on Clinical Trials.gov (NCT04264624). Clinical relevance: Subgingival air-polishing could help re-establishing a eubiotic microbioma in deep bleeding periodontal pockets after initial non-surgical treatment.

The present parallel randomized clinical trial aimed to assess, after a 3-year follow-up period, whether the choice of surgical technique-either manual or guided-and of the operator - non-expert operator or skilled - can affect the stability of peri‑implant marginal bone levels in implants placed 1 mm sub-crestal. Patients received platform-switched implants (Anyridge, MegaGen Implant Co., Gyeongbuk, South Korea) featuring a 5-degree internal conical connection and supporting single screw-retained fixed crowns. The implants were randomly assigned to be placed through a digitally static guided surgery procedure (Test group - GS) or a freehand surgical technique (Control Group - FH). A non-expert operator (fewer than 20 implants placed in his professional activity) was selected to perform procedures for the GS Group, while a skilled operator (with over 1000 implants placed in his professional activity) was chosen for the FH Group. Marginal bone level (MBL) was measured at prosthesis installation (t0) and at 1 (t1), 2 (t2) and 3 years (t3) of follow-up. Changes in MBL from t0 to t3 were analyzed through periapical radiographs. Moreover, MBL changes at all time points were correlated to different supra-crestal soft tissue heights (STH): less than 3 and ≥ 3 mm, respectively. 60 implants in 18 patients were examined, with 30 implants allocated to the GS group and 30 to the FH group. The difference in MBL change between the two groups was 0.11 ± 0.22 mm, which was not statistically significant (p = 0.61). At the time of prosthetic loading, the mean MBL for implants with STH less than 3 mm was 0.33 mm higher than implants with STH ≥ 3 mm, though this difference was not statistically significant (P = 0.065). Digitally static guided implant placement, performed by a non-expert operator, does not limit marginal bone remodeling, when compared to a freehand procedure performed by an experienced operator. After correct and careful planning, early marginal bone levels (MBL) around conical connection, platform-switched implants placed sub-crestally may be stable in time. Digital planning and surgery have the potential to assist non-expert clinicians in achieving implant placements with comparable outcomes to those performed by experts.