Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most devastating diseases affecting rice production worldwide, resulting in significant yield losses and threatening global food security. The severity of rice blast, particularly in susceptible regions, underscores the urgent need for available effective resistance strategies. In this study, six sets of gene-specific molecular markers for the Pik locus associated with rice blast resistance were developed based on publicly available gene sequences. Experimental validation confirmed their high accuracy. During the marker development process, a novel haplotype of the Pik locus was identified. This haplotype is characterized by 14 bp mutations and a 9 bp insertion within the coding sequence region when compared to the Pikh allele. Subsequently, a molecular marker specific to this haplotype was developed and validated. The application of these seven sets of markers to analyze 163 japonica rice cultivars bred in Yunnan Province between 1980 and 2020 revealed that 38.65% of the cultivars carry the Piks allele, indicating a low resistance frequency against the rice blast fungus under field conditions. In contrast, only a small proportion of cultivars possess other Pik locus alleles, which exhibit higher resistance frequencies. These findings highlight the limited utilization of Pik locus genes in japonica rice breeding in Yunnan. Furthermore, 21.47% of the cultivars lack any of the aforementioned Pik locus alleles, indicating the genetic diversity and complexity of the rice genetic resources of Yunnan Province.

Cross-border e-commerce platforms are one of the important channels for Chinese green tea to be sold globally, and the description of green tea products is a key factor affecting its sales. Therefore, optimizing the translation of product descriptions will positively promote the overseas sales of Chinese green tea. With a quantitative comparative analysis based on a self-built small comparable corpus, this study identified similarities and differences between the translated texts offered by Chinese vendors and the native texts offered by English-speaking vendors in the aspect of product information and textual functions, and it is proposed that the translator could resort to native texts as references to optimize their translation of product descriptions for online shops on cross-border e-commerce platforms.