Abiotic stresses are significant environmental issues that restrict plant growth, productivity, and survival while also posing a threat to global food production and security. Plants produce compatible solutes known as osmolytes to adapt themselves in such changing environment. Osmolytes contribute to homeostasis maintenance, provide the driving gradient for water uptake, maintain cell turgor by osmotic adjustment, and redox metabolism to remove excess level of reactive oxygen species (ROS) and reestablish the cellular redox balance as well as protect cellular machinery from osmotic stress and oxidative damage. Perceiving the mechanisms how plants interpret environmental signals and transmit them to cellular machinery to activate adaptive responses is important for crop improvement programs to get stress–tolerant varieties. A large number of studies conducted in the last few decades have shown that osmolytes accumulate in plants and have strong associations with abiotic stress tolerance. Production of abundant osmolytes is needed for tolerance in many plant species. In addition, transgenic plants overexpressing genes for different osmolytes showed enhanced tolerance to various abiotic stresses. Many important aspects of their mechanisms of action are yet to be largely identified, especially regarding the relevance and relative contribution of specific osmolytes to the stress tolerance of a given species. Therefore, more efforts and resources should be invested in the study of the abiotic stress responses of plants in their natural habitats. The present review focuses on the possible roles and mechanisms of osmolytes and their association toward abiotic stress tolerance in plants. This review would help the readers in learning more about osmolytes and how they behave in changing environments as well as getting an idea of how this knowledge could be applied to develop stress tolerance in plants.

Climate change intensifies biotic and abiotic stresses, threatening global crop productivity. High-throughput phenotyping (HTP) technologies provide a non-destructive approach to monitor plant responses to environmental stresses, offering new opportunities for both crop stress resilience and breeding research. Innovations, such as hyperspectral imaging, unmanned aerial vehicles, and machine learning, enhance our ability to assess plant traits under various environmental stresses, including drought, salinity, extreme temperatures, and pest and disease infestations. These tools facilitate the identification of stress-tolerant genotypes within large segregating populations, improving selection efficiency for breeding programs. HTP can also play a vital role by accelerating genetic gain through precise trait evaluation for hybridization and genetic enhancement. However, challenges such as data standardization, phenotyping data management, high costs of HTP equipment, and the complexity of linking phenotypic observations to genetic improvements limit its broader application. Additionally, environmental variability and genotype-by-environment interactions complicate reliable trait selection. Despite these challenges, advancements in robotics, artificial intelligence, and automation are improving the precision and scalability of phenotypic data analyses. This review critically examines the dual role of HTP in assessment of plant stress tolerance and crop performance, highlighting both its transformative potential and existing limitations. By addressing key challenges and leveraging technological advancements, HTP can significantly enhance genetic research, including trait discovery, parental selection, and hybridization scheme optimization. While current methodologies still face constraints in fully translating phenotypic insights into practical breeding applications, continuous innovation in high-throughput precision phenotyping holds promise for revolutionizing crop resilience and ensuring sustainable agricultural production in a changing climate.

Water scarcity leads to significant ecological challenges for global farming production. Sustainable agriculture depends on developing strategies to overcome the impacts of drought on important crops, including soybean. In this present study, seven promising soybean genotypes were evaluated for their drought tolerance potential by exposing them to water deficit conditions. The control group was maintained at 100% field capacity (FC), while the drought-treated group was maintained at 50% FC on a volume/weight basis. This treatment was applied at the second trifoliate leaf stage and continued until maturity. Our results demonstrated that water shortage exerted negative impacts on soybean phenotypic traits, physiological and biochemical mechanisms, and yield output in comparison with normal conditions. Our results showed that genotype G00001 exhibited the highest leaf area plant⁻¹ (483.70 cm²), photosynthetic attributes like stomatal conductance (gs) (0.15 mol H₂O m⁻² s⁻¹) and photosynthetic rate (Pn) (13.73 μmol CO₂ m⁻² s⁻¹), and xylem exudation rate (0.25 g h⁻¹) under drought conditions. The G00001 genotype showed greater leaf greenness by preserving photosynthetic pigments (total chlorophylls (Chls) and carotenoids; 4.23 and 7.34 mg g⁻¹ FW, respectively) in response to drought conditions. Soybean plants accumulated high levels of stress indicators like proline and malondialdehyde when subjected to drought stress. However, genotype G00001 displayed lower levels of proline (4.49 μg g⁻¹ FW) and malondialdehyde (3.70 μmol g⁻¹ FW), indicating that this genotype suffered from less oxidative stress induced by drought stress compared to the other investigated soybean genotypes. Eventually, the G00001 genotype had a greater yield in terms of seeds pod⁻¹ (SP) (1.90) and 100-seed weight (HSW) (14.60 g) under drought conditions. On the other hand, BD2333 exhibited the largest decrease in plant height (37.10%), pod number plant⁻¹ (85.90%), SP (56.20%), HSW (54.20%), gs (90.50%), Pn (71.00%), transpiration rate (59.40%), relative water content (34.40%), Chl a (79.50%), total Chls (72.70%), and carotenoids (56.70%), along with the maximum increase in water saturation deficit (290.40%) and malondialdehyde content (280.30%) under drought compared to control conditions, indicating its higher sensitivity to drought stress. Our findings suggest that G00001 is a promising candidate to consider for field trials and further evaluation of its molecular signature may help breeding other elite cultivars to develop drought-tolerant, high-yielding soybean varieties.

Rice (Oryza sativa) is a major crop and a main food for a major part of the global population. Rice species have derived from divergent agro-climatic regions, and thus, the local germplasm has a large genetic diversity. This study investigated the relationship between phenotypic and genetic variabilities of yield and yield-associated traits in Aus rice to identify short-duration, high-yielding genotypes. Targeting this issue, a field experiment was carried out to evaluate the performance of 51 Aus rice genotypes, including 50 accessions in F₅ generation and one short-duration check variety BINAdhan-19. The genotypes exhibited a large and significant variation in yield and its associated traits, as evidenced by a wide range of their coefficient of variance. The investigated traits, including days to maturity (DM), plant height (PH), panicle length (PL) and 1000-grain weight (TW) exhibited a greater genotypic coefficient of variation than the environmental coefficient of variation. In addition, the high broad-sense heritability of DM, PH, PL and TW traits suggests that the genetic factors significantly influence the observed variations in these traits among the F₅ Aus rice accessions. This study also revealed that the grain yield per hill (GY) displayed a significant positive correlation with PL, number of filled grains per panicle (FG) and TW at both genotype and phenotype levels. According to the hierarchical and K-means cluster analyses, the accessions BU-R-ACC-02, BU-R-ACC-08 and R2-36-3-1-1 have shorter DM and relatively higher GY than other Aus rice accessions. These three accessions could be employed in the ongoing and future breeding programs for the improvement of short-duration and high-yielding rice cultivars.