Introduction: Gibberellins (GA) are terpenoids that serve as important plant hormones by acting as growth and response modulators against injuries and parasitism. In this study, we investigated the in vitro anti-NF-κB, anti-Candida, and antioxidant activity of gibberellin A4 (GA4) and A7 (GA7) compounds, and further determined their toxicity in vivo. Methods: GA4 and GA7 in vitro toxicity was determined by MTT method, and nontoxic concentrations were then tested to evaluate the GA4 and GA7 anti-NF-κB activity in LPS-activated RAW-luc macrophage cell culture (luminescence assay). GA4 in silico anti-NF-κB activity was evaluated by molecular docking with the software “AutoDock Vina”, “MGLTools”, “Pymol”, and “LigPlot+”, based on data obtained from “The Uniprot database”, “Protein Data Bank”, and “PubChem database”. The GA4 and GA7 in vitro anti-Candida effects against Candida albicans (MYA 2876) were determined (MIC and MFC). GA7 was also evaluated regarding the viability of C. albicans preformed biofilm (microplate assay). In vitro antioxidant activity of GA4 and GA7 was evaluated against peroxyl radicals, superoxide anions, hypochlorous acid, and reactive nitrogen species. GA4 and GA7 in vivo toxicity was determined on the invertebrate Galleria mellonella larvae model. Results: Our data show that GA4 at 30 µM is nontoxic and capable of reducing 32% of the NF-κB activation on RAW-luc macrophages in vitro. In vitro results were confirmed via molecular docking assay (in silico), since GA4 presented binding affinity to NF-κB p65 and p50 subunits. GA7 did not present anti-NF-κB effects, but exhibited anti-Candida activity with low MIC (94 mM) and MFC (188 mM) values. GA7 also presented antibiofilm properties at 940 mM concentration. GA4 did not present anti-Candida effects. Moreover, GA4 and GA7 showed antioxidant activity against peroxyl radicals, but did not show scavenging activity against the other tested radicals. Both compounds did not affect the survival of G. mellonella larvae, even at extremely high doses (10 g/Kg). Conclusion: Our study provides preclinical evidence indicating that GA4 and GA7 have a favorable low toxicity profile. The study also points to GA4 and GA7 interference with the NF-κB via, anti-Candida activity, and a peroxyl radical scavenger, which we argue are relevant biological effects.

Os cirurgiões-dentistas se utilizam de medicamentos para o tratamento e prevenção de problemas orais, o que torna necessário um certo conhecimento para a correta indicação do uso na doença do paciente. A Hipertensão, o Diabetes Mellitus e a paciente Gestante se apresentam com alta prevalência entre os pacientes do consultório, desse modo, o dentista precisa estar preparado quanto aos seus sintomas e sinais os quais possam apresentar para atender esses pacientes. Dessa forma o objetivo do estudo foi indicar o perfil dos cirurgiões-dentistas em relação aos anestésicos locais utilizados e à terapêutica medicamentosa para hipertensos, diabéticos e gestantes e investigar se a conduta dos cirurgiões-dentistas está de acordo com a literatura odontológica. Foram entrevistados cirurgiões-dentistas que cursam pós-graduação em odontologia no UNIFUNEC que responderam a 24 questões, entre elas, questões pessoais e sobre a prática profissional no atendimento clínico de pacientes especiais. Os dados obtidos foram divididos em certos e errados e registrados no Software Microsoft Excel em tabelas elaboradas e estão esclarecidos nos resultados e na discussão. Participaram da pesquisa 36 alunos, sendo que a maioria era do sexo feminino. Com relação ao número de acertos nas questões, o percentual foi em torno de 65%, bem semelhante ao da literatura. Do total das questões, as que apresentaram um percentual menor de 50% de acerto foram as relacionadas à prescrição medicamentosa e anestesia local ao paciente diabético. Com este trabalho, podemos concluir que a compreensão dos dentistas no atendimento clínico de pacientes hipertensos, diabéticos e gestantes foi em torno de 76%. DENTAL APPOINTMENT FOR HYPERTENSIVE, DIABETIC, AND PREGNANT PATIENTS: BACKGROUND AND PRACTICE REVIEW OF DENTAL SURGEONS ABSTRACT Dental surgeons use drugs to treat and prevent oral disorders; it requires a certain level of knowledge in order to prescribe proper medication according to the patient’s disease. Hypertension, Diabetes Mellitus, and Pregnancy are very common among patients and have high prevalence in dental offices, thus, dentists must be aware of the symptoms and signs which may be presented by patients in order to assist them. Therefore, this paper aims to highlight the dental surgeons’ profile regarding local anesthetic applied as well as drug therapy for hypertensive, diabetic and pregnant women, also investigate if the dental surgeons’ practice is in compliance with dental literature. Dental surgeons who attended post-graduation degrees at UNIFUNEC were interviewed and they were asked to answer 24 questions, among them, questions related to their personal and clinical professional practice for those particular patients. All obtained data were classified as right and wrong and recorded on Microsoft Excel Software in designed tables which are explained in the results and discussion. Thirty-six scholars participated in this research, most were female. Regarding the number of right questions, the percentage was 65%, coequal to literature. From the total, questions presenting less than 50% accuracy were related to drug prescription and local anesthetic for diabetic patients. In light of this paper, we may conclude that clinical dentists’ background for hypertensive, diabetic, and pregnant patients was around 76%. Descriptors: Hypertension; Diabetes Mellitus; Pregnant Women.

Introdução: Os pacientes, diabéticos é um público comum no consultório. Os cirurgiões dentistas fazem uso e prescrevem medicamentos que auxiliam na prevenção e cura de doenças orais, tornando necessário um conhecimento apropriado para a correta indicação de uma determinada doença. Dessa forma, o objetivo do presente estudo é descrever a compreensão dos acadêmicos do Curso de Odontologia do Unifunec sobre seus conhecimentos e o manejo de pacientes com hipertensão, diabetes e gestantes em relação aos anestésicos locais utilizados e à terapêutica medicamentosa. Material e Métodos: Os alunos matriculados no quinto ano do curso de odontologia foram convidados a responder 22 questões sobre perfil pessoal e a prática clínica de atendimento odontológico dos pacientes especiais. Os dados obtidos foram divididos em certos e errados e registrados no Software Microsoft Excelem tabelas elaboradas. Resultados: As tabelas e os gráficos estão exemplificados nos resultados e na discussão. Participaram da pesquisa 68 alunos, sendo que a maioria era do sexo feminino, atendem em clínica escola 100% dos alunos entrevistados. A maioria das questões obteve uma porcentagem de acertos favorável ao da literatura com algumas questões chegando a 90%. Do total de perguntas, as que apresentaram um percentual de acerto menor de 50% foram as questões sobre a anestesia local no paciente diabético e gestante. Conclusão: A compreensão dos graduandos sobre a consulta odontológica de pacientes hipertensos, diabéticos e gestantes encontrou-se em torno de 56%. UNDERGRADUATE STUDENT’S KNOWLEDGE ABOUT DENTAL APPOINTMENT FOR HYPERTENSIVE, DIABETIC AND PREGNANT PATIENTS ABSTRACT Introduction: Hypertensive and diabetic patients are frequent in dental offices. Dental surgeons make use and prescribe medications which help in the prevention and cure of oral diseases; it requires appropriate knowledge in order to properly write prescriptions to certain diseases. Consequently, this paper aims at describing the understanding of the undergraduate students’ Dentistry School at Unifunec about their knowledge and management of hypertensive, diabetic, and pregnant patients regarding the use of local anesthetic and drug therapy. Material and methods: Undergraduate students who were enrolled in the fifth year of Dental School were invited to answer 22 questions about personal profile and clinical practice for those particular patients’ dental care. The obtained data was classified as right and wrong and recorded at the Software Microsoft Excel spreadsheets. Results: Spreadsheets and graphics are exemplified in results and discussion. Sixty-eight undergraduate students participated in the study, most of them were female, 100% of them assisting patients at a dental school clinic. Most of the questions obtained a favorable percentage of correctness in accordance to the literature with some questions hitting 90% correctness. From the total of the questions, questions about local anesthetics for diabetic and pregnant patients obtained less than 50% correctness. Conclusion: The understanding of the undergraduate students about dental appointment for hypertensive, diabetic, and pregnant patients was found to be around 56%. Keywords: Hypertension. Diabetes Mellitus. Pregnant women.