Different microbial electrochemical technologies are being developed for many diverse applications, including wastewater treatment, biofuel production, water desalination, remote power sources, and biosensors. Current and energy densities will always be limited relative to batteries and chemical fuel cells, but these technologies have other advantages based on the self-sustaining nature of the microorganisms that can donate or accept electrons from an electrode, the range of fuels that can be used, and versatility in the chemicals that can be produced. The high cost of membranes will likely limit applications of microbial electrochemical technologies that might require a membrane. For microbial fuel cells, which do not need a membrane, questions about whether larger-scale systems can produce power densities similar to those obtained in laboratory-scale systems remain. It is shown here that configuration and fuel (pure chemicals in laboratory media vs actual wastewaters) remain the key factors in power production, rather than the scale of the application. Systems must be scaled up through careful consideration of electrode spacing and packing per unit volume of the reactor.

Anaerobic ammonium oxidation (anammox) bacteria contribute significantly to the global nitrogen cycle and play a major role in sustainable wastewater treatment. Anammox bacteria convert ammonium (NH₄⁺) to dinitrogen gas (N₂) using intracellular electron acceptors such as nitrite (NO₂⁻) or nitric oxide (NO). However, it is still unknown whether anammox bacteria have extracellular electron transfer (EET) capability with transfer of electrons to insoluble extracellular electron acceptors. Here we show that freshwater and marine anammox bacteria couple the oxidation of NH₄⁺ with transfer of electrons to insoluble extracellular electron acceptors such as graphene oxide or electrodes in microbial electrolysis cells. ¹⁵N-labeling experiments revealed that NH₄⁺ was oxidized to N₂ via hydroxylamine (NH₂OH) as intermediate, and comparative transcriptomics analysis revealed an alternative pathway for NH₄⁺ oxidation with electrode as electron acceptor. Complete NH₄⁺ oxidation to N₂ without accumulation of NO₂⁻ and NO₃⁻ was achieved in EET-dependent anammox. These findings are promising in the context of implementing EET-dependent anammox process for energy-efficient treatment of nitrogen.

Recycling CO2 into organic products through microbial electrosynthesis (MES) is attractive from the perspective of circular bioeconomy. However, several challenges need to be addressed before scaling-up MES systems. In this review, recent advances in electrode materials, microbe-catalyzed CO2 reduction and MES energy consumption are discussed in detail. Anode materials are briefly reviewed first, with several strategies proposed to reduce the energy input for electron generation and enhance MES bioeconomy. This was followed by discussions on MES cathode materials and configurations for enhanced chemolithoautotroph growth and CO2 reduction. Various chemolithoautotrophs, effective for CO2 reduction and diverse bioproduct formation, on MES cathode were also discussed. Finally, research efforts on developing cost-effective process for bioproduct extraction from MES are presented. Future perspectives to improve product formation and reduce energy cost are discussed to realize the application of the MES as a chemical production platform in the context of building a circular economy.