Biophotovoltaics is a relatively new discipline in microbial fuel cell research. The basic idea is the conversion of light energy into electrical energy using photosynthetic microorganisms. The microbes will use their photosynthetic apparatus and the incoming light to split the water molecule. The generated protons and electrons are harvested using a bioelectrochemical system. The key challenge is the extraction of electrons from the microbial electron transport chains into a solid-state anode. On the cathode, a corresponding electrochemical counter reaction will consume the protons and electrons, e.g., through the oxygen reduction to water, or hydrogen formation. In this review, we are aiming to summarize the current state of the art and point out some limitations. We put a specific emphasis on cyanobacteria, as these microbes are considered future workhorses for photobiotechnology and are currently the most widely applied microbes in biophotovoltaics research. Current progress in biophotovoltaics is limited by very low current outputs of the devices while a lack of comparability and standardization of the experimental set-up hinders a systematic optimization of the systems. Nevertheless, the fundamental questions of redox homeostasis in photoautotrophs and the potential to directly harvest light energy from a highly efficient photosystem, rather than through oxidation of inefficiently produced biomass are highly relevant aspects of biophotovoltaics.

The microbial fuel cell (MFC) is a promising environmental biotechnology that has been proposed mainly for power production and wastewater treatment. Though small power output constrains its application for directly operating most electrical devices, great progress in its chemical, electrochemical, and microbiological aspects has expanded the applications of MFCs into other areas such as the generation of chemicals (e.g., formate or methane), bioremediation of contaminated soils, water desalination, and biosensors. In recent decades, MFC-based biosensors have drawn increasing attention because of their simplicity and sustainability, with applications ranging from the monitoring of water quality (e.g., biochemical oxygen demand (BOD), toxicants) to the detection of air quality (e.g., carbon monoxide, formaldehyde). In this review, we summarize the status quo of MFC-based biosensors, putting emphasis on BOD and toxicity detection. Furthermore, this review covers other applications of MFC-based biosensors, such as DO and microbial activity. Further, challenges and prospects of MFC-based biosensors are briefly discussed.

Pseudomonas putida is a promising host for the bioproduction of chemicals, but its industrial applications are significantly limited by its obligate aerobic character. The aim of this paper is to empower the anoxic metabolism of wild-type Pseudomonas putida to enable bioproduction anaerobically, with the redox power from a bioelectrochemical system (BES). The obligate aerobe Pseudomonas putida F1 was able to survive and produce almost exclusively 2–Keto-gluconate from glucose under anoxic conditions due to redox balancing with electron mediators in a BES. 2-Keto-gluconate, a precursor for industrial anti-oxidant production, was produced at an overall carbon yield of over 90 % based on glucose. Seven different mediator compounds were tested, and only those with redox potential above 0.207 V (vs standard hydrogen electrode) showed interaction with the cells. The productivity increased with the increasing redox potential of the mediator, indicating this was a key factor affecting the anoxic production process. P. putida cells survived under anaerobic conditions, and limited biofilm formation could be observed on the anode’s surface. Analysis of the intracellular pools of ATP, ADP and AMP showed that cells had an increased adenylate energy charge suggesting that cells were able to generate energy using the anode as terminal electron acceptor. The analysis of NAD(H) and NADP(H) showed that in the presence of specific extracellular electron acceptors, the NADP(H) pool was more oxidised, while the NAD(H) pool was unchanged. This implies a growth limitation under anaerobic conditions due to a shortage of NADPH and provides a way to limit biomass formation, while allowing cell maintenance and catalysis at high purity and yield. For the first time, this study proved the principle that a BES-driven bioconversion of glucose can be achieved for a wild-type obligate aerobe. This non-growth bioconversion was in high yields, high purity and also could deliver the necessary metabolic energy for cell maintenance. By combining this approach with metabolic engineering strategies, this could prove to be a powerful new way to produce bio-chemicals and fuels from renewables in both high yield and high purity.

The degradation of waste organics through microbial electrolysis cell (MEC) generates hydrogen (H2) gas in an economically efficient way. MEC is known as the advanced concept of the microbial fuel cell (MFC) but requires a minor amount of supplementary electrical energy to produce H2 in the cathode microenvironment. Different bio/processes could be integrated to generate additional energy from the substrate used in MECs, which would make the whole process more sustainable. On the other hand, the energy required to drive the MEC mechanism could be harvested from renewable energy sources. These integrations could advance the efficiency and economic feasibility of the whole process. The present review critically discusses all the integrations investigated to date with MECs such as MFCs, anaerobic digestion, microbial desalination cells, membrane bioreactors, solar energy harvesting systems, etc. Energy generating non-biological and eco-friendly processes (such as dye-sensitized solar cells and thermoelectric microconverters) which could also be integrated with MECs, are also presented and reviewed. Achieving a comprehensive understanding about MEC integration could help with developing advanced biorefineries towards more sustainable energy management. Finally, the challenges related to the scaling up of these processes are also scrutinized with the aim to identify the practical hurdles faced in the MEC processes.