This study aimed to verify the presence ofListeria monocytogenes,Salmonellaspp., andEscherichia coliin two Brazilian swine slaughterhouses, as well as to perform antibiograms, detect virulence and antimicrobial resistance genes, and evaluate thein vitrobiofilm-forming capability of bacterial isolates from these environments. OneSalmonellaTyphi isolate and 21E.coliisolates were detected, whileL.monocytogeneswas not detected.S. Typhi was isolated from the carcass cooling chamber’s floor, resistant to several antimicrobials, including nalidixic acid, cefazolin, chloramphenicol, doxycycline, streptomycin, gentamicin, tetracycline, and sulfonamide, and contained resistance genes, such astet(B),tet(C),tet(M), andampC. It also showed moderate biofilm-forming capacity at 37°C after incubating for 72 h. The prevalence of the 21E.coliisolates was also the highest on the carcass cooling chamber floor (three of the four samplings [75%]). TheE.coliisolates were resistant to 12 of the 13 tested antimicrobials, and none showed sensitivity to chloramphenicol, an antimicrobial prohibited in animal feed since 2003 in Brazil. The resistance genesMCR-1,MCR-3,sul1,ampC,clmA,cat1,tet(A),tet(B), andblaSHV, as well as the virulence genesstx-1,hlyA,eae,tir α,tir β,tir γ, andsaawere detected in theE.coliisolates. Moreover, 5 (23.8%) and 15 (71.4%)E.coliisolates presented strong and moderate biofilm-forming capacity, respectively. In general, the biofilm-forming capacity increased after incubating for 72 h at 10°C. The biofilm-forming capacity was the lowest after incubating for 24 h at 37°C. Due to the presence of resistance and virulence genes, multi-antimicrobial resistance, and biofilm-forming capacity, the results of this study suggest a risk to the public health as these pathogens are associated with foodborne diseases, which emphasizes the hazard of resistance gene propagation in the environment.