Brisingida Fisher 1928 is one of the seven currently recognised starfish orders, and one of the least known taxa as being exclusive deep-sea inhabitants. Modern deep-sea expeditions revealed their common occurrences in various deep-sea settings including seamounts, basins and hydrothermal vent peripheral, underlining the necessity of clarifying their global diversity and phylogeny. In this study, we present a comprehensive molecular phylogeny of Brisingida which encompasses the highest taxonomic diversity to date. DNA sequences (COI, 16S, 12S and 28S) were obtained from 225 specimens collected in the global ocean, identified as 58 species spanning 15 of the 17 extant genera. Phylogenetic relationship was inferred using both maximum likelihood and Bayesian inference methods, revealing polyphyletic families and genera and indicating nonnegligible bias in prior morphology-based systematics. Based on the new phylogeny, a novel classification of the order, consisting of 5 families and 17 genera, is proposed. Families Odinellidae, Brisingasteridae and Novodiniidae (sensu Clark and Mah, 2001) were resurrected to encompass the genera Odinella, Brisingaster and Novodinia. Brisingidae and Freyellidae were revised to include 11 and 3 genera, respectively. A new genus and species, two new subgenera and seven new combinations are described and a key to each genus and family is provided. Transformations of morphological traits were evaluated under the present phylogenetic hypothesis. A series of paedomorphic characters were found in many genera and species, which led to a high degree of homoplasy across phylogenetically distant groups. Our results provide new insights in the phylogeny and ontogeny of the order, and highlight the necessity to evaluate character convergence under sound phylogenetic hypothesis.

Zooplankton plays a crucial role in marine ecosystems. However, due to issues with morphological identification and sampling methods, knowledge of their biodiversity and community structure in the vast pelagic zone of the western tropical Pacific Ocean is still limited compared with other areas in the North Pacific Ocean. In this study, we used environmental DNA (eDNA) metabarcoding and morphological identification approaches to investigate mesozooplankton’s biodiversity and vertical assemblages in the western tropical Pacific Ocean from the surface to 1000 m deep. Two different barcodes were used in metabarcoding: mitochondrial cytochrome oxidase I (COI) and small subunit ribosomal RNA genes (18S). Our results showed that eDNA metabarcoding revealed 2-4 times more medusae taxa than morphological processing. Both methods detected that small copepod species were dominant in the community and six of the top-10 most abundant copepod genera overlapped. Morphological results showed that mesozooplankton communities were classified into three groups: epipelagic (0–200 m), upper mesopelagic (200-500 m), and lower mesopelagic (500–1000 m). And eDNA results showed distinct vertical variations of mesozooplankton communities within the epipelagic. Both methods showed that the dominant genera Clausocalanus, Paracalanus, and Calocalanus exhibited diel patterns in relative abundance, demonstrating that the eDNA method can capture diel vertical migration (DVM) signals of copepods. Overall, our results indicate that eDNA metabarcoding is an effective and efficient tool for the study and monitoring of mesozooplankton diversity and vertical assemblages in the open sea.

Several squat lobster specimens of the genus Munidopsis were collected from an artificially placed carcass fall (cow bones) on Weijia Guyot in the western Pacific Ocean. Based on morphological comparisons and molecular analysis, three specimens were confirmed as juveniles of M. albatrossae Pequegnat & Pequegnat, 1973, which represents the first record of this species in the western Pacific. The other specimens collected are newly described as Munidopsis spinifrons sp. nov., which is distinguished from the closely related species in having a spinose rostrum and basal lateral eyespine on the eyestalk. The M. albatrossae from Weijia Guyot exhibited very low genetic distances when compared with a conspecific sample from Monterey Bay, USA, and the closely related species M. aries (A. Milne Edwards, 1880) from the northeastern Atlantic. A phylogenetic tree based on the mtCOI gene shows M. spinifrons sp. nov. as sister to M. vrijenhoeki Jones & Macpherson, 2007 and M. nitida (A. Milne Edwards, 1880), although M. vrijenhoeki presents a complex relationship with other species in the clade. The systematic status of the new species and the closely related species are discussed.

A Gram-negative, aerobic, neutrophilic, rod-shaped bacterium, designated strain CN85^T, was isolated from a sediment sample collected from the East China Sea and was subjected to a polyphasic taxonomic characterization. This isolate grew in the presence of 0.5–6.0 % (w/v) NaCl and at 20–40 °C; optimum growth was observed with 3 % (w/v) NaCl and at 35 °C. Chemotaxonomic analysis showed that Q-8 was the predominant respiratory quinone and that iso-C_15 : 0, iso-C_11 : 0 3-OH, iso-C_17 : 1ω9c, iso-C_17 : 0, iso-C_11 : 0 and C_16 : 0 were the major fatty acids. The G+C content of the genomic DNA was 57.8 mol%. On the basis of 16S rRNA gene sequence analysis, the isolate was affiliated to the genus Microbulbifer. Strain CN85^T exhibited most phylogenetic affinity with respect to the type strain of Microbulbifer maritimus (97.0 % sequence similarity) and showed less than 97 % sequence similarity with respect to other described Microbulbifer species with known 16S rRNA gene sequences. The DNA–DNA hybridization between strain CN85^T and M. maritimus JCM 12187^T was 44 %. On the basis of phenotypic and genotypic data, strain CN85^T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer donghaiensis sp. nov. is proposed. The type strain is CN85^T (=CGMCC 1.7063^T =JCM 15145^T).

The Clarion-Clipperton Fracture Zone (CCFZ) is located in the northeastern equatorial Pacific and contains abundant polymetallic nodules. To investigate its bacterial diversity, four libraries of 16S rRNA genes were constructed from sediments of four stations in different areas of the CCFZ. In total, 313 clones sequenced from the 4 libraries were assigned into 14 phylogenetic groups and 1 group of 28 unclassified bacteria. High bacterial diversity was predicted by the rarefaction analysis. The most dominant group overall was Proteobacteria, but there was variation in each library: Gammaproteobacteria was the most dominant group in two libraries, E2005-01 and ES0502, while Alphaproteobacteria and Deltaproteobacteria were the most dominant groups in libraries EP2005-03 and WS0505, respectively. Seven groups, including Alphaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, Betaproteobacteria, Acidobacteria, Actinobacteria, and Bacteroidetes, were common to all four libraries. The remaining minor groups were distributed in libraries with different patterns. Most clones sequenced in this study were clustered with uncultured bacteria obtained from the environment, such as the ocean crust and marine sediment, but only distantly related to isolates. Bacteria involved in the cycling of metals, sulfur and nitrogen were detected, and their relationship with their habitat was discussed. This study sheds light on the bacterial communities associated with polymetallic nodules in the CCFZ and provides primary data on the bacterial diversity of this area.