Honey bees use pollen and nectar from flowers to produce food. Because they often forage on crops, they are at risk of being exposed to plant protection products (PPPs), both directly and in stored food. Due to the adverse effects of synthetic PPPs on pollinators, biopesticides may be a viable alternative. Common tansy extract is used as one of the natural substitutes for synthetic pesticides. In our study, the effect of fermented common tansy extract on aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and gamma-glutamyl transpeptidase (GGTP) activity and the concentration of triglycerides (TGs), total protein (TP), total antioxidant status (TAS), and glucose in honey bee workers’ hemolymph was assessed. These biochemical markers give valuable information about the immunity, detoxification, and nutrition of a bee’s body. Caged bees were given tansy extract added at various concentrations in sugar syrup for 24 h. Then, they were provided with only sugar syrup. After 7 days of the experiment, hemolymph was collected and analyzed. We observed changes in the activity of AST, ALT, GGTP enzymes and TG, TP, and glucose levels, but not all changes were statistically significant. In terms of AST activity, statistically significant differences were found. All groups tested, including the negative control group, showed reduced enzyme activity values compared to the positive control group. In TG concentration, differences were observed between the groups receiving 2% extract and 1% ethanol. Glucose levels differed between the groups receiving 1% extract and 2% extract and between the positive control group and 1% extract. Bee body proper functioning is affected by changes in enzyme activity, especially those responsible for immunity and detoxification, such as AST, ALT, ALP, and GGTP. Despite the short time of bees’ exposure to the agent, the results of study show visible effects. Our results provide a basis for further research on the impact of tansy extract on honey bees.

The development of wireless technology and the desire to improve communication electromagnetic fields (EMFs) of various frequencies have become common across the honey bee’s foraging landscape. There has been discussion for many years about the possible impact of electromagnetic fields on living organisms. Artificial radio fields emit frequencies ranging from 100 kHz to 300 GHz. The presented research aimed to demonstrate the influence of the radiofrequency electromagnetic field (RF-EMF) with a frequency of 900 MHz on the behavior of honey bees in laboratory conditions. For this experiment, we used wooden cages to house honey bee workers immediately after they emerged. Bee workers were divided into control and experimental groups. Bees in the control group were not exposed to RF fields, while the experimental groups were exposed to 900 MHz electromagnetic fields of different intensities and durations of exposure. Bees’ behavior was analyzed with an appropriate computer program. Behavioral analysis of bees was performed immediately after exposure and seven days after exposure. Our research has shown that the radio field (900 MHz) affects the behavior of bees compared to the control group, although not all results are statistically significant. Significant effects were observed seven days after exposure in walking, flight, and individual contact. However, it is worth extending the study to include the impact of an RF-EMF on the expression of genes responsible for bee behavior.