Antibiotic residue in meat is a serious public health concern due to its harmful effects on consumer health. This study aimed at estimating the residue levels of four commonly used antibiotics in meat samples using three analytical methods (ELISA, TLC and HPLC). A total of 150 samples of raw meat from sales points were analysed for ciprofloxacin, streptomycin, tetracycline, and sulphanilamide residues. Overall, ELISA analysis showed that 56, 34, 18, and 25.3% of the samples tested positive for ciprofloxacin, streptomycin, sulphanilamide and tetracycline residues respectively while TLC and HPLC detected 21.4, 29.4, 92.5, and 14.6%, and 8.3, 41.1, 88.8, and 14.6% of the samples as containing the residues, with ciprofloxacin and sulphanilamide having the lowest and highest occurrence, respectively. Furthermore, the concentrations of antibiotic residues were in the ranges of 19.8–92.8, 26.6–489.1, 14.2–1280.8, and 42.6–355.6 μg/kg with ELISA, while HPLC detected concentration ranges of 20.7–82.1, 41.8–320.8, 65.2–952.2 and 32.8–95.6 μg/kg for sulphanilamide, tetracycline, streptomycin, and ciprofloxacin, respectively. Mean ciprofloxacin and streptomycin residue levels were above the Codex/SA MRL recommended limit, while 3% of the samples contained multidrug residues. Although some of the mean residues levels were below the permissible limits, the co-occurrence of multidrug residues in some of the samples calls for concern.

Little is known of the mycobiota present in ready-to-eat peanuts consumed in Southern Africa. Knowledge of the mycobiota and aflatoxigenic species can elucidate potential health risks associated with consumption of ready-to-eat peanuts sold by supermarkets and roadside vendors. We investigated the culturable mycobiota diversity in supermarket and roadside-vended peanuts as well as the presence of five aflatoxin biosynthesis pathway–related genes (aflR, aflJ, aflM, aflD, and aflP) in 15 suspected aflatoxigenic isolates, with a focus on Mafikeng, South Africa. Mean colony-forming unit (CFU) counts of 288.7 and 619.7 CFU/g were observed in supermarket and roadside-vended peanuts, respectively. A total of 145 fungal isolates comprising 26 distinct taxa (based on 97% internal transcribed spacer region [ITS1-5.8S-ITS2] sequence similarity) were obtained, including strains representing Aspergillus, Acremonium, Alternaria, Bipolaris, Chaetomium, Ectophoma, Epicoccum, Hamigera, Leancillium, Monascus, Penicillium, Periconia, Talaromyces, and Trichoderma. Phylogenetic analyses of concatenated sequences of the ITS1-5.8S-ITS2, β-tubulin, and calmodulin genes delineated the species of Aspergillus, which included A. flavus, A. fumigatus, A. hiratsukae, A. niger, and A. parasiticus. Higher species richness was obtained from supermarket peanuts compared with roadside-vended peanuts, with eight species common to both sources. Across supermarket or roadside-vended peanuts, A. fumigatus, A. niger, and A. flavus were prevalent (>40% incidence). In contrast, strains related to or representing Ectophoma multirostata, Aspergillus hiratsukae, Bipolaris zeae, Chaetomium bostrychodes, Epicoccum nigrum, Hamigera paravellanea, Lecanicillium aphanocladii, Monascus ruber, Periconia macrospinosa, Periconia lateralis, Talaromyces funiculosus, Talaromyces minioluteus, Talaromyces wortmannii, Talaromyces spp., and Trichoderma sp. were detected in either supermarket or roadside-vended peanuts. Among the five aflatoxin biosynthesis pathway–related genes, aflD and aflM were more prevalent (87%) and aflR was the least prevalent (40%). Findings suggest that roasted peanuts meant for human consumption and sold at supermarkets and by roadside vendors are contaminated with potential toxin-producing fungi. Hence, proper processing and packaging of peanuts before vending is recommended.

Goat milk could be used to reduce malnutrition since it is highly nutritious, and many people in rural communities in South Africa rear small ruminants for survival. However, the risk of food contamination by antibiotic residues is one of the significant problems facing public health, and is a result of the irresponsible use of veterinary drugs. One hundred goat farmers were interviewed using a questionnaire, and raw milk samples from 266 goats were collected and analysed for the presence of antibiotic residues. Screening for amoxicillin, tetracycline, sulfamethazine, erythromycin, and streptomycin residues was done using the enzymelinked immunosorbent assay (ELISA) while high-performance liquid chromatography (HPLC) was carried out for confirmation. The questionnaire shows that all (100%) of the participants acknowledged the use of antibiotics on their goats and 99% of them were aware of the possibility of antibiotic residues in milk. ELISA results for residues of erythromycin, sulfamethazine and amoxicillin exceeded the Codex Alimentarius maximum residue levels (MRLs) in 94.7%, 82.3%, and 35.3% of analysed samples, respectively. Tetracycline was present in all (100%) analysed milk samples, and streptomycin was detected in 18.7% of samples; however, these results were below the recommended MRLs. The HPLC method confirmed the presence of streptomycin and tetracycline residues in 90% and 40% of the samples analysed. However, the concentrations were below the accepted MRL standards. Approximately 76.6% of samples exceeded the established MRL for sulfamethazine and 10% for erythromycin. Amoxicillin was not detected by the HPLC method. The results obtained in this study indicate a high level of contamination of goat milk with antibiotic residues, which may harm the health of the consumers.

Background and Aim: Leptospirosis is one of the major emerging global economic and health problems affecting donkeys, thereby reducing their work output. Furthermore, the disease has public health importance because of its zoonotic nature. Despite the significant contribution donkeys make to the national economy, less attention is given to diseases that afflict donkeys and reduce their productivity and performance. A cross-sectional study was conducted to investigate the seroprevalence of Leptospira and identify risk factors associated with the occurrence of the disease among donkeys in the study area. Materials and Methods: A questionnaire survey was used to collect the following data: Demographic, environmental, management, and health-related factors. Blood samples were aseptically collected from 365 randomly selected donkeys from 19 villages. The sera were tested using the microscopic agglutination test. Categorical variables were summarized and presented as proportions and their 95% confidence interval (CI). A binary logistic regression model was fitted to the data to identify risk factors associated with Leptospira seroprevalence in donkeys within the study areas. Results: The majority of the donkeys (29.6%; n=108/365) were from Mafikeng local municipality, and the rest (19.7%; n=72/365) were from Ratlou. Just over half (58.1%; n=212/365) of the donkeys tested were female, and the remaining (41.9%; n=153/365) were males. In addition, most donkeys (42.7%; n=156/365) were between 6 and 12 years old, followed by those between 0 and 5 years (37%; n=135/365), and only 20.3% (n=74/365) were above 12 years. Out of the donkeys tested, 11.5% (95% CI: 4.86-18.14) donkeys tested positive for Leptospira antibodies. The most common serovar was Bratislava (81%; n=34/42), followed by Tarassovi (19.04%; n=8/42). While gender was positively associated with seroprevalence of the disease (Adjusted Odds Ratio [AOR]=4.88; p=0.0001), the presence of horses (AOR=0.226; p=0.002) and agricultural activities (AOR=0.093; p=0.0001) in the vicinity of the dwellings of the donkeys were negatively associated with Leptospira seropositivity in the study area. Conclusion: Findings reported here show that donkeys in the study area are reservoirs for the predominant serovar Bratislava and the less dominant serovar Tarassovi. The gender of the donkey was a risk factor for Leptospira seroprevalence. Further studies are needed to investigate the role of agricultural activities in the vicinity of the dwellings of donkeys on the occurrence of Leptospira in the study area.

This study aimed to examine fungi diversity in dried beef meat sold in Ekiti State, characterize the isolated fungi, and determine the aflatoxin-producing ability of the Aspergillus fungi in the samples. Dried beef meat was collected from different markets in Ekiti State and screened for the presence of filamentous fungi using molecular methods. Samples were cultured aseptically on potato dextrose agar (PDA) for fungi isolation, and molecular identification was performed using DNA extraction, Polymerase chain Reaction (PCR), ITS-1/ITS-4 primer pair, and nucleotide sequencing. The results obtained indicated a range of filamentous fungi genera including Aspergillus, Rhizopus, Penicillium, Fusarium, Cladosporium, Alternaria, and other fungi species contaminating the dried meat at (43%), (42%), (3%), (2%), (2%), (1%), and (7%), respectively. High incidences were recorded for Aspergillus flavus, Aspergillus niger, and Aspergillus fumigatus in most of the screened samples. Aspergillus flavus accounted for (24.7%) of all the Aspergillus species isolated with the presence of the gene needed for aflatoxin production. The occurrences of these filamentous fungal species pose a cause for concern, as most of these fungal species are known producers of certain toxic substances. Maximum likelihood phylogenetic analysis showed a high similarity index score, which indicated a good relationship between isolated Aspergillus Species and the closely related strains from GenBank, isolated from different sources and countries. The implication of this study is that consumer health may be at risk through exposure to contaminated dried meat.