Raptors, including eagles, are geographically widespread and sit atop the food chain, thereby serving an important role in maintaining ecosystem balance. After facing population declines associated with exposure to organochlorine insecticides such as dichlorodiphenyltrichloroethane (DDT), bald eagles (Haliaeetus leucocephalus) have recovered from the brink of extinction. However, both bald and golden eagles (Aquila chrysaetos) are exposed to a variety of other toxic compounds in the environment that could have population impacts. Few studies have focused on anticoagulant rodenticide (AR) exposure in eagles. Therefore, the purpose of this study was to determine the types of ARs that eagles are exposed to in the USA and better define the extent of toxicosis (i.e., fatal illness due to compound exposure). Diagnostic case records from bald and golden eagles submitted to the Southeastern Cooperative Wildlife Disease Study (University of Georgia) 2014 through 2018 were reviewed. Overall, 303 eagles were examined, and the livers from 116 bald eagles and 17 golden eagles were tested for ARs. The percentage of AR exposure (i.e., detectable levels but not associated with mortality) in eagles was high; ARs were detected in 109 (82%) eagles, including 96 (83%) bald eagles and 13 (77%) golden eagles. Anticoagulant rodenticide toxicosis was determined to be the cause of mortality in 12 (4%) of the 303 eagles examined, including 11 bald eagles and 1 golden eagle. Six different AR compounds were detected in these eagles, with brodifacoum and bromadiolone most frequently detected (81% and 25% of eagles tested, respectively). These results suggest that some ARs, most notably brodifacoum, are widespread in the environment and are commonly consumed by eagles. This highlights the need for research to understand the pathways of AR exposure in eagles, which may help inform policy and regulatory actions to mitigate AR exposure risk.

The Cerulean Warbler (Setophaga cerulea) is a declining Nearctic–Neotropical migratory songbird of conservation concern. Implementing full annual cycle conservation strategies to facilitate recovery has been difficult because we know little about the migratory period or strength of migratory connectivity between North American breeding and South American nonbreeding regions. Between 2014 and 2017, we deployed geolocators on 282 males at 14 study sites throughout the species’ range to (1) evaluate the strength and pattern of connectivity between breeding and nonbreeding regions, (2) identify approximate routes and stopover regions, and (3) document migration phenology. We obtained data from 26 birds and observed moderate migratory connectivity overall but documented strong parallel migration for birds breeding in two longitudinally disparate regions. Most (14 of 15; 93%) Appalachian breeders spent the stationary nonbreeding period in the Colombian/Venezuelan Andes, whereas most (5 of 7; 71%) Ozark-breeders spent the stationary nonbreeding period in Peru/Ecuador. The majority of spring migration (62%) was spent in Central America at multiple stopover locations between Panama and southern Mexico. The 2 migratory periods were approximately equal in duration: 38 ± 2 days (SE) in fall and 42 ± 2 days (SE) in spring. Based on the observed connectivity pattern, conservation of Appalachian-breeding populations during the stationary nonbreeding period should focus on forest conservation and restoration in pre-montane/lower montane forests of Colombia and Venezuela, whereas Ozark-breeding population conservation should focus on forest conservation and restoration efforts in Ecuador and Peru. Further conservation efforts are also needed on the breeding grounds, especially for the most sharply declining populations. And finally, conservation of forests used by Cerulean Warblers during stopover periods throughout Central America and southern Mexico, in southeastern United States coastal areas, and in the Mississippi Alluvial Valley will benefit individuals from multiple breeding locations and populations. • Many bird species that migrate between North and South America are declining in population and to inform conservation strategies we must identify where vulnerable populations spend time at different points in the year. • Cerulean Warblers are declining forest songbirds that breed in eastern North America and travel annually to the tropical Andes of South America, where they spend ~ 6 months per year. • We captured and equipped Cerulean Warblers with light-level sensors (geolocators) to track their annual movements. • Birds from the east of the breeding range tended to travel to the eastern nonbreeding range in the Andes of Colombia and Venezuela and birds from the west of the breeding range tended to travel to the south-western portions of the nonbreeding range in Peru and Ecuador. • We also identified the extensive use of migratory stopover locations (rest/refueling) in Central America and southern Mexico. • Our findings are important for informing the management and conservation of this species by highlighting the importance of stopover regions and connecting specific breeding and nonbreeding regions.

Anticoagulant rodenticides (ARs) are widely used across North America to control rodent infestations but may cause direct mortality or nonlethal effects when secondarily consumed by raptors. Barn Owls (Tyto alba) are at high risk for secondary consumption because they specialize in rodent prey and often live in human-made structures. We investigated the exposure of Barn Owls in Kentucky, US, to ARs and to dicoumarol, an anticoagulant compound naturally found in certain moldy forages. We tested the liver tissue of 48 Barn Owl carcasses collected during 2012–16. We confirmed exposure to one or more ARs in 33% of the birds examined and detected dicoumarol in 13% of the samples. Rodenticides detected included brodifacoum, coumachlor, and bromadiolone. The prevalence of detected exposure to brodifacoum for after-hatch-year birds (65%) was significantly (P=0.012) higher than hatch-year birds (22%). Brodifacoum was the most commonly detected AR, found in 88% of AR-positive birds. The pesticide registration for this chemical in the US was canceled in 2015 for general consumer products, which likely resulted in a decreasing rate of detected exposure to brodifacoum during our study. We present these results as an example of secondary exposure rates during a period when a pesticide has been restricted and then removed from the consumer market.

Haliaeetus leucocephalus (Bald Eagle) is a well-studied species, and information on their nest abundance and population trends is widely available. However, research on the movements and home range of nesting Bald Eagles in the interior US is lacking. In this study, we used a solar-powered Argos platform transmitter terminal (PTT) with global positioning system (GPS) capabilities to monitor the movements of an adult, breeding male Bald Eagle, captured at Ballard Wildlife Management Area, KY, from 29 April 2012 to 23 October 2013. We generated minimum convex polygons (MCP) for comparisons to previous studies and used 95% and 50% fixed kernel density estimators (KDE) to estimate home-range size and core-use areas, respectively, during nesting and non-nesting periods. Estimates of home range and core-use areas were slightly larger during the non-nesting period (MCP = 420.0 km2, 95% KDE = 1.6 km2, 50% KDE = 0.2 km2) when compared to the nesting period (nesting MCP=415.0 km2, 95% KDE =1.3 km2, 50% KDE = 0.01 km2). Our home range and core-use estimates were smaller than previous studies using comparable methods, suggesting that habitat quality at this site may be high.