This study focused on evaluating the effect of different concentrations of nutrients and total suspended solids on the removal rate of nutrients and biocompounds from the macroalgae U. lactuca in an integrated system with the shrimp Penaeus vannamei in biofloc. The experiment lasted 45 days and included five treatments with three replicates each, with percentages of 0 (control), 25, 50, 75, and 100% biofloc inoculum (73.3 ± 5.7 and 325.0 ± 21.2 mg L⁻¹ initial nitrate and solids, respectively, in the 100% inoculum), from a shrimp farm, resulting in different concentrations of solids and nutrients. The macroalgae were introduced into 280 L tanks at a density of 0.88 kg m⁻², along with 200 shrimp m⁻³. The algae were separated by a floating structure. Water quality parameters were measured, and the nutrient removal rate was evaluated. The treatment with 75% inoculum showed a removal rate of 55.0 ± 4.0 and 31.0 ± 10.0% of nitrate and phosphate, respectively. There was no difference in macroalgae growth between the treatments; however, macroalgae grown in 75% inoculum had higher protein, chlorophyll-a, and lower ash values compared with the control. The use of macroalgae in integrated production with shrimp under the conditions of the treatment with 75% biofloc inoculum proved to be viable and sustainable.