The purpose of this study was to analyze midwifery practice in the Mazahua and Otomi (MO) indigenous community and its relationship with the persistence of the native language and the use of medicinal plants. A semi-structured survey with qualitative and quantitative information was designed and validated. Data were collected from the students enrolled in the Intercultural Health Degree at the Intercultural University of the State of Mexico, their parents, their grandparents, and people from their community of the MO ethnic group. The variables mother tongue and births attended by midwives among both MO and non-indigenous people were correlated with three generations (grandparents, parents, and grandchildren). In the MO indigenous group, births attended by midwives and mother tongue concerning the three generations were lost in proportions of 25.5% and 17.05%, respectively. There are 23% more midwife-attended births in the MO community than among non-ethnic people. The medicinal plants most used by the MO indigenous group are “too” (Montanoa tomentosa Cerv.) and “lengua de vaca” (Rumex crispus L.). As regards family economy, 79.3% of the adults surveyed consider that there is a saving of between 25% and 75% with midwifery practice in the MO community. There is a generational correspondence between the loss of native language and midwifery practice.