The guaraná tree is a species known for the stimulating properties found in its seeds, leading to increased consumption and promising production. Cultivation is primarily carried out in family farming systems, and one of the challenges in the production process is the acquisition of seedlings. The conventional nursery system, which employs intermittent misting for the production of guaraná clones, raises the cost of seedlings, necessitating the exploration of alternatives to this approach. The aim of this study was to evaluate different conditions as alternatives to the conventional nursery system for rooting guaraná cuttings. The experimental design was entirely randomized, featuring three guaraná genotypes, BRS Maués, BRS CG 611, and BRS Luzeia, alongside two rooting conditions, the conventional nursery and a humid chamber with nebulization, with four repetitions. The rooting of guaraná clones was significantly influenced by the interaction between conditions and genotypes. The humid chamber with nebulization demonstrated superior root system characteristics among the tested conditions, with a rooting rate of 56.66% for BRS Luzeia. This method proved to be efficient and accessible for small producers, making it a satisfactory option for rooting guaraná trees.