Alstroemeria spp. and carnation (Dianthus caryophyllus L.) have considerable and increasing economic importance in the floriculture market, therefore breeders carry out intense breeding programs to select new superior varieties. However, poor germination of hybrid seeds remains a bottleneck. Based on this assumption, seed pre-treatments and in vitro germination protocols, using different germination substrates, were applied in Alstroemeria spp. and carnation to improve germinability. Seed viability was tested using the 2,3,5-triphenyl tetrazolium chloride (TTC) test, and resulted in 91.10% ± 2.33 and 86.66% ± 3.85 in Alstroemeria and carnation, respectively. In Alstroemeria, pre-treatment with potassium nitrate (KNO₃) in combination with modified ½ Murashige and Skoog (MS) medium ensured high germination uniformity combined with high germination percentage, showing significantly higher values than the control. In carnation, a suitable seed sterilization procedure was set up (up to 95.8% sterility); treatments with gibberellic acid (GA₃) and KNO₃ did not influence germination percentage compared to the control. A high multiplication rate of seedling lines was obtained on hormone-free MS medium.