Abiotic stresses such as drought, heat or salinity are major causes of yield loss worldwide. Recent studies have revealed that the acclimation of plants to a combination of different environmental stresses is unique and therefore cannot be directly deduced from studying the response of plants to each of the different stresses applied individually. The efficient detoxification of reactive oxygen species (ROS) is thought to play a key role in enhancing the tolerance of plants to abiotic stresses. Here, we report on the role of melatonin in the protection of the photosynthetic apparatus through the increase in ROS detoxification in tomato plants grown under the combination of salinity and heat, two of the most common abiotic stresses known to act jointly. Plants treated with exogenous melatonin showed a different modulation in the expression on some antioxidant-related genes and their related enzymes. More specifically, ascorbate peroxidase, glutathione reductase, glutathione peroxidase and phospholipid hydroperoxide glutathione peroxidase (APX, GR, GPX and Ph-GPX, resepctively) showed an antagonistic regulation as compared to plants that did not receive melatonin. This translated into a better antioxidant capacity and to a lesser ROS accumulation under stress combination. The performance of the photosynthesis parameters and the photosystems was also increased in plants treated with exogenous melatonin under the combination of salinity and heat. In accordance with these findings, tomato plants treated with melatonin were found to grow better under stress combination that the non-treated ones. Our study highlights the important role that exogenous melatonin plays in the acclimation of plants to a combination of two different abiotic stresses, and how this compound can specifically regulate oxidative stress-related genes and enzymes to increase plant tolerance.

K(+) uptake in the high-affinity range of concentrations and its components have been widely studied. In Arabidposis thaliana, the AtHAK5 transporter and the AtAKT1 channel have been shown to be the main transport proteins involved in this process. Here, we study the role of these two systems under two important stress conditions: low K(+) supply or the presence of salinity. T-DNA insertion lines disrupting AtHAK5 and AtAKT1 are employed for long-term experiments that allow physiological characterization of the mutant lines. We found that AtHAK5 is required for K(+) absorption necessary to sustain plant growth at low K(+) in the absence as well as in the presence of salinity. Salinity greatly reduced AtHAK5 transcript levels and promoted AtAKT1-mediated K(+) efflux, resulting in an important impairment of K(+) nutrition. Although having a limited capacity, AtHAK5 plays a major role for K(+) acquisition from low K(+) concentrations in the presence of salinity.

Summary: Salinization of agricultural lands is a major threat to agriculture. Many different factors affect and determine plant salt tolerance. Nonetheless, there is a consensus on the relevance of maintaining an optimal cytosolic potassium : sodium ion (K⁺ : Na⁺) ratio for salinity tolerance in plants. This ratio depends on the operation of plasma membrane and tonoplast transporters. In the present review we focus on some aspects related to the energetic cost of maintaining that K⁺ : Na⁺ ratio. One of the factors that affect the cost of the first step of K⁺ acquisition – root K⁺ uptake through High Affinity K⁺ transporter and Arabidopsis K⁺ transport system 1 transport systems – is the value of the plasma membrane potential of root cells, a parameter that may differ amongst plant species. In addition to its role in nutrition, cytosolic K⁺ also is important for signalling, and K⁺ efflux through gated outward‐rectifying K⁺ and nonselective cation channels can be regarded as a switch to redirect energy towards defence reactions. In maintaining cytosolic K⁺, the great buffer capacity of the vacuole should be considered. The possible role of high‐affinity K⁺ transporters (HKT)2s in mediating K⁺ uptake under saline conditions and the importance of cycling of K⁺ throughout the plant also are discussed.

Summary: The occurrence of radiocesium in food has raised sharp health concerns after nuclear accidents. Despite being present at low concentrations in contaminated soils (below μm), cesium (Cs⁺) can be taken up by crops and transported to their edible parts. This plant capacity to take up Cs⁺ from low concentrations has notably affected the production of rice (Oryza sativa L.) in Japan after the nuclear accident at Fukushima in 2011. Several strategies have been put into practice to reduce Cs⁺ content in this crop species such as contaminated soil removal or adaptation of agricultural practices, including dedicated fertilizer management, with limited impact or pernicious side‐effects. Conversely, the development of biotechnological approaches aimed at reducing Cs⁺ accumulation in rice remain challenging. Here, we show that inactivation of the Cs⁺‐permeable K⁺ transporter OsHAK1 with the CRISPR‐Cas system dramatically reduced Cs⁺ uptake by rice plants. Cs⁺ uptake in rice roots and in transformed yeast cells that expressed OsHAK1 displayed very similar kinetics parameters. In rice, Cs⁺ uptake is dependent on two functional properties of OsHAK1: (i) a poor capacity of this system to discriminate between Cs⁺ and K⁺; and (ii) a high capacity to transport Cs⁺ from very low external concentrations that is likely to involve an active transport mechanism. In an experiment with a Fukushima soil highly contaminated with ¹³⁷Cs⁺, plants lacking OsHAK1 function displayed strikingly reduced levels of ¹³⁷Cs⁺ in roots and shoots. These results open stimulating perspectives to smartly produce safe food in regions contaminated by nuclear accidents.