The present study enumerates the attenuating effects of curcumin and α-tocopherol against propoxur induced oxidative DNA damage in human peripheral blood mononuclear cells (PBMC). Cultured cells were isolated from peripheral blood of healthy volunteers, and were exposed to varying concentrations of propoxur (0–21 μg/ml) for 6, 12, and 24 h, and in combination with curcumin (9.2 μg/ml) or α-tocopherol (4.3 μg/ml) or both. Cytotoxic effect of propoxur was examined by MTT assay. The role of oxidative stress beneath the cytotoxicity of propoxur was evaluated by the measurement of reduced glutathione (GSH), malondialdehyde (MDA) and 8-hydroxy-2′-deoxyguanosine (8-OH-dG) levels in cell lysate. A concentration-dependent cell death, depletion of GSH, an increase in the level of both MDA and 8-OH-dG were observed. Co-treatment with curcumin or α-tocopherol significantly attenuates depleted GSH, decrease in MDA and 8-OH-dG levels in propoxur exposed cells (p < 0.05). The results of the present study provide experimental evidence of involvement of oxidative stress in propoxur-mediated genotoxicity in human PBMC and highlight the antioxidant role of curcumin and α-tocopherol following propoxur exposure.

This research outlines that the manufacture of palladium nanoparticles (PdNPs) as being straight forward, inexpensive, and environmentally friendly. Rosa damascena flower extract was successfully used in this investigation to reduce palladium nanoparticles (PdNPs). UV-VIS, FTIR, XRD, TEM, EDX, DLS, and SEM were used to the characterized of the biologically formed of PdNPs. Upon UV-visible irradiation of physico-chemically produced PdNPs, the SPR peak was measured at 360 nanometers. The TEM investigation indicated that the produced palladium nanoparticles had a spherical form and a diameter of around 50 nm. The biologically synthesized PdNPs demonstrated notable antimicrobial activity, including antifungal activity. PdNPs shows antifungal activity against some fungal species such as Aspergillus niger, Aspergillus flavus and Candida albicans. Based on findings, zone of inhibition of fungal strains is less than from fungal strains with Fluconazole. Bio-inspired production of PdNPs allows for adaptability to fungus strains. This makes PdNPs more suitable for biomedical applications.

Endosulfan, a well-known organochlorine pesticide, induces apoptosis and depletion of reduced glutathione (GSH) in human peripheral blood mononuclear cells (PBMC). Thus, for the amelioration of its effect, antioxidant and antiapoptotic potential of curcumin was evaluated. For ascertaining the attenuating effect of curcumin, various biochemical indices of cell damage such as cytotoxicity, oxidative stress, apoptosis (phosphatidylserine externalization, DNA fragmentation, and cytochrome c) in human PBMC was evaluated following endosulfan exposure (0–100 µM). To assess the role of HSP27 on endosulfan-induced apoptosis, the expression of HSP27 was examined. Curcumin (25 µM) increased cell viability significantly. As evident from the restoration of GSH, antiapoptotic potential was directly proportional to their antioxidant nature of curcumin. The present study indicates that the beneficial effect of curcumin on endosulfan-induced cytotoxicity is related to the induced synthesis of HSP27, emphasizing its antioxidant and therapeutic potential as well as underscoring the mechanism of pesticide-induced toxicity at cellular level. Taken together, these findings suggest that curcumin protects against endosulfan-induced immunotoxicity in human PBMC by attenuating apoptosis.

Aim: To carry out a case–control study of the association of GST gene polymorphisms in pediatric asthma-related oxidative stress. Materials & methods: Asthma patients (n = 250) and age-matched heal...