This study investigated the potential of Cranberry extract to stimulate osteogenesis in vitro. The total phenolic and monomeric anthocyanin contents in the Cranberry were determined. Liquid chromatography coupled with mass spectrometry was used to identify the Cranberry’s constituents. To assess the Cranberry’s cytotoxicity, a thiazolyl blue tetrazolium bromide assay was employed. Concerning the osteogenesis potential of Cranberry, alkaline phosphatase (ALP) activity, bone morphogenetic protein 2 (BMP-2) expression, and extracellular matrix mineralization were evaluated. The total phenolic content was 522.72 ± 9.80 mg GAE g-1 ES and 364.95 ± 12.49 mg GAE/g detected by the Fast Blue BB and Folin–Ciocalteu method, respectively. For monomeric anthocyanin, the content was 460 ± 30 mg ECG g-1 ES. Moreover, Cranberry concentrations ranged from 62.5 to 500 mg/mL and were found to be biocompatible with osteoblasts and mesenchymal stromal cells. Regarding osteogenesis, 20 mg/mL of Cranberry promoted 2-fold more ALP activity and almost 1.5-fold more BMP-2 than compared to the positive control group. Additionally, 200 mg/mL of Cranberry stimulated a 1.7-fold increase in extracellular matrix mineralization compared to the positive control group. In conclusion, Cranberry displayed potential in stimulating early and late markers of osteogenesis. Its ability to promote osteogenesis and its biocompatibility at higher concentrations hold promise for future application into biomaterials for bone regeneration.

Objectives: Despite the increasing scientific evidence regarding the application of Cranberries in dentistry, a comprehensive understanding of their potential benefits, active constituents, and mechanisms of action remains lacking. Consequently, this narrative review aims to meticulously analyze and consolidate the existing scientific literature on the utilization of Cranberries for the prevention and treatment of oral diseases. Materials and Methods: Electronic databases (PubMed, Scopus, and Web of Science) were searched up to October 2025. This review included in vitro, in vivo, and clinical research studies. A two-phase selection process was carried out. In phase 1, two reviewers independently screened titles and abstracts to identify potentially eligible studies. In phase 2, the same reviewers performed the full-text assessments of the eligible articles. Results: Among the 93 eligible articles, most assessed Cranberry use in Cariology (n = 28) and Periodontics (n = 26). Biofilm and microbial virulence factors (n = 46) were the most frequently studied topics. Cranberry extract (n = 32) and high-molecular-weight non-dialyzable material (NDM) (n = 23) were the most evaluated Cranberry fractions. Overall, Cranberry-derived compounds were identified as non-toxic and demonstrated promising antimicrobial activity against dental caries-related microorganisms in preclinical studies (n = 20). Regarding periodontal and peri-implant diseases, Cranberry demonstrated host immune modulator effects, counteracting the inflammatory and destructive mechanisms (n = 8). Additionally, Cranberries presented benefits in reducing the inflammation associated with periodontal disease and temporal mandibular joint lesions (n = 1). Regarding dental erosion, Cranberry inhibited dentin erosion (n = 4); however, no effect was observed on enamel lesions (n = 2). As an antioxidant agent, Cranberry showed effectiveness in preventing dental erosion (n = 18). Beyond that, Cranberry neutralized reactive oxygen species generated immediately after dental bleaching, enhancing bond strength (n = 2) and counteracting the oxygen ions formed on the tooth surface following bleaching procedures (n = 3). In osteoclastogenesis assays, A-type proanthocyanidins inhibited bone resorption (n = 1). In osteogenic analysis, preservation of hydroxycarbonate apatite deposition and an increase in early and late osteogenic markers were observed (n = 2). Conclusions: Cranberry bioactive compounds, both individually and synergistically, exhibit substantial potential for diverse applications within dentistry, particularly in the prevention and management of oral and maxillofacial diseases. This review provides insights into the plausible incorporation of Cranberries in contemporary dentistry, offering readers an informed perspective on their potential role.

Objective: To identify factors associated with the use of dental implants in Brazilian adults and older adults. Methods: This cross-sectional analytical study utilized population-based data from the 2023 National Oral Health Survey. The sample comprised adults (35–44 years) and older adults (65–74 years). Two dichotomous outcomes were modeled: self-reported implant use (yes/no) and clinically observed presence of implants (none/one or more). Adjusted odds ratios (ORs) were estimated using multiple logistic regression for complex samples, with demographic and socioeconomic factors, type of dental service used, and number of missing teeth as independent variables. Results: The prevalence of dental implants ranged from approximately one-tenth to one-fifth of the sample, depending on the method of identification. In the adjusted analysis for self-reported use, older adults, individuals with higher income, those who used private dental services, and those who sought care for treatment or rehabilitation had significantly higher odds of reporting implant use. Conversely, when analyzing clinically observed implants, only the number of permanent teeth lost showed a significant association. Conclusion: The use of dental implants in the Brazilian population reflects both the accessibility of dental services and the clinical need for rehabilitation. Using different identification methods provides a more comprehensive understanding of the distribution of implants across the country. These findings can allow the refinement of clinical and organizational criteria for the provision of implant-supported oral rehabilitation within the public system, thereby promoting greater equity and sustainability.

Muitos experimentos in vitro podem ser conduzidos para determinar a inocuidade de substâncias e biomateriais para serem empregados como agentes terapêuticos e cosméticos, lembrando que estes idealmente não podem apresentar citotoxicidade para desempenharem suas funções no organismo humano. Assim, novos fármacos, cosméticos, aditivos alimentares e biomateriais devem ter seu efeito citotóxico testado antes de serem liberados para uso humano. Historicamente, estes testes incluíam um grande número de experimentação em animais; porém, atualmente há vários métodos alternativos elaborados pela Organização para Cooperação e Desenvolvimento Econômico (OCDE) que foram reconhecidos pelo Conselho Nacional de Controle de Experimentação Animal (CONCEA) e pela Agência Nacional de Vigilância Sanitária (ANVISA) no Brasil. Adicionalmente, a comunidade científica internacional continua se esforçando para seguir o princípio dos 3Rs em relação ao uso de animais, ao mesmo tempo que instituições públicas e privadas vêm alocando recursos humanos e financeiros para desenvolver novas metodologias in vitro. Deste modo, no presente capítulo serão abordados os principais métodos destinados à avaliação de citotoxicidade in vitro. Acredita-se que a adoção destes métodos alternativos possa contribuir para a aceleração do processo de avaliação de novos produtos, com potencial redução de tempo e de custos para que sejam colocados à disposição da população

RESUMO Objetivo: Identificar fatores associados a desfechos de reabilitação com implantes dentários em adultos e idosos no Brasil. Métodos: Estudo transversal, analítico, com dados de base populacional da Pesquisa Nacional de Saúde Bucal, 2023. A amostra incluiu adultos (35–44 anos) e idosos (65–74 anos). Foram modelados dois desfechos dicotômicos: autorrelato de uso de implante (sim/não) e presença clínica de implante (nenhum/um ou mais). As Razões de Chances (RC) ajustadas foram estimadas por meio de regressão logística múltipla para amostras complexas, considerando como variáveis independentes fatores demográficos e socioeconômicos, uso de serviços e número de dentes perdidos. Resultados: A prevalência de implantes variou entre aproximadamente um décimo e um quinto da amostra, dependendo do método de identificação. Idosos, indivíduos com maior renda, usuários de serviços privados e aqueles que buscaram atendimento por tratamento ou reabilitação apresentaram significativamente maior chance de relatar uso de implante. Na análise da presença clínica, observou-se associação apenas com o número de dentes permanentes perdidos. Conclusão: O uso de implantes dentários na população brasileira reflete o acesso aos serviços odontológicos e a necessidade clínica de reabilitação. Diferentes formas de identificação ampliam a compreensão sobre o padrão de distribuição dos implantes no país. Os achados oferecem subsídios para o aprimoramento de critérios clínicos e organizacionais para a oferta de reabilitação oral implantossuportada no sistema público, que favoreçam a equidade e a sustentabilidade.