Scientific mapping using bibliometric data network analysis was applied to analyze research works related to lipases and their industrial applications, evaluating the current state of research, challenges, and opportunities in the use of these biocatalysts, based on the evaluation of a large number of publications on the topic, allowing a comprehensive systematic data analysis, which had not yet been conducted in relation to studies specifically covering lipases and their industrial applications. Thus, studies involving lipase enzymes published from 2018 to 2022 were accessed from the Web of Science database. The extracted records result in the analysis of terms of bibliographic compatibility among the articles, co-occurrence of keywords, and co-citation of journals using the VOSviewer algorithm in the construction of bibliometric maps. This systematic review analysis of 357 documents, including original and review articles, revealed studies inspired by lipase enzymes in the research period, showing that the development of research, together with different areas of knowledge, presents good results related to the applications of lipases, due to information synchronization. Furthermore, this review showed the main challenges in lipase applications regarding increased production and operational stability; establishing well-defined evaluation criteria, such as cultivation conditions, activity, biocatalyst stability, type of support and reactor; thermodynamic studies; reuse cycles; and it can assist in defining goals for the development of successful large-scale applications, showing several points for improvement of future studies on lipase enzymes.

This study investigated the effect of low-cost carbon and nitrogen sources on fructosyltransferase (FTase) production by Aspergillus oryzae IPT-301, aiming to optimize the enzymatic synthesis of fructooligosaccharides (FOS), prebiotic compounds valued for their bifidogenic effects. FTase is a key enzyme in transfructosylation, the central step in FOS production. To reduce production costs, Very High Polarization (VHP) and Demerara (DM) sugars were evaluated as carbon sources, while sodium nitrate (NaNO₃), ammonium sulfate (NH₄)₂SO₄, and urea were tested as nitrogen sources. FTase production, both extracellular and intracellular, was conducted under submerged fermentation at 30 °C and 200 rpm for 72 h. DM sugar outperformed VHP, increasing extracellular and intracellular transfructosylation activity (A_T) by 2.3-fold and 2.1-fold, respectively. Among nitrogen sources, NaNO₃ was most effective in DM-containing media, yielding 1.6–2.0 times higher extracellular A_T and up to 4.7 times greater intracellular activity compared to other nitrogen sources. These findings suggest that the combination of DM sugar and NaNO₃ significantly enhances FTase yield, providing a cost-effective strategy for industrial-scale FOS production.

Anais do CBCP 2020 - Congresso on-line Brasileiro de Tecnologia de Cereais e Panificação (978-65-88243-73-2) - Caracterização Da Farinha Do Caroço De Açaí (Euterpe Oleracea )E Aplicação Tecnológica

Hidrolisados enzimáticos são os produtos obtidos pela hidrólise proteica, que quebram ligações peptídicas, gerando peptídeos de diferentes tamanhos e aminoácidos livres. Os peptídeos podem ser obtidos por fontes como animais, plantas e microrganismos. A partir dessas, a bioatividade adquirida através da hidrólise agrega maior valor ao hidrolisado. Dentre as propriedades bioativas, destacam-se os efeitos antioxidante e antibacteriano, que contribuem para a saúde humana e preservação dos alimentos. A composição química varia em função do substrato, das condições de hidrólise e da purificação aplicada. Quando as proteínas são hidrolisadas, aumentam sua solubilidade e digestibilidade, podendo facilitar a absorção de nutrientes, pois os peptídeos e aminoácidos resultantes são geralmente de tamanho menor e mais facilmente absorvidos pelo trato gastrointestinal. Os hidrolisados são de extrema importância e por isso muito explorados pela indústria alimentícia, que pode utilizá-los para o melhoramento de texturas, aumento do valor nutricional e até mesmo realçar o sabor. DOI:https://doi.org/10.56238/tecnolocienagrariabiosoci-038

Fructooligosaccharides are prebiotic sugars that are widely used in the production of functional foods, which can be produced enzymatically by the transfructosylation reaction of sucrose. This work aimed to optimize the production of an invertase with high transfructosylation activity from Aspergillus carbonarius PC-4 using pineapple crown as the inducer substrate and evaluate its biochemical properties. The culture medium was optimized using a Plackett–Burman experimental design and a central composite rotatable design, resulting in a maximum transfructosylation activity of 65.33 U/mL at 72 h of cultivation. The cultivation parameters were Yp/s = 1070.75 U/g and P_P = 2771.48 U/h, which showed an increase of 5.2-fold in the enzyme produced. The optimum temperature (50 °C) and pH (5.0) for the enzymatic activity were obtained by a CCR design. The enzyme showed a half-life of 60 min at 40 °C. In conclusion, the invertase produced from A. carbonarius PC-4 using agro-industrial waste (pineapple crown) and an inorganic nitrogen source (ammonium nitrate) exhibits high transfructosylation activity that can be used as a potential source for the production of fructooligosaccharides.