The thin layers in the ocean are temporally-coherent aggregations of phytoplankton with high concentrations at small vertical scales, presenting important hotspots of ecological activity. Lidar could identify thin phytoplankton layers at a large spatial scale due to its capabilities of profile detection with a high efficiency. However, studies that linked thin layers to environmental factors are few, which limits our understanding of the layer formation mechanism. This paper investigates the characteristics and formation conditions of thin phytoplankton layers in the northern Gulf of Mexico using airborne lidar. The results depict that the chlorophyll concentration determines the formation probability of the phytoplankton layer. The layer is mainly formed at concentrations less than 6 mg m⁻³ and mostly distributed at 2 mg m⁻³. In addition, layer thicknesses were within 5 m and layer depths were mainly in the range of 10–15 m. Layer depths in the nearshore region were shallower than those in the offshore region. We conclude that the characteristics and formation conditions of the thin phytoplankton layers depend on the nutrients and light that are related to the seabed topography, turbidity, eddies and upwelling. The findings of this paper will enhance the understanding of layer formation mechanisms.

The diel vertical variations of chlorophyll a (Chl-a) concentration are thought of primarily as an external manifestation of regulating phytoplankton’s biomass, which is essential for dynamically estimating the biogeochemical cycle in inland waters. However, information on these variations is limited due to insufficient measurements. Undersampled observations lead to delayed responses in phytoplankton assessment, impacting accurate evaluations of carbon export and water quality in dynamic inland waters. Here, we report the first lidar-observed diel vertical variations of inland Chl-a concentration. Strong agreement with r² of 0.83 and a root mean square relative difference (RMSRD) of 9.0% between the lidar-retrieved and in situ measured Chl-a concentration verified the feasibility of the Mie–fluorescence–Raman lidar (MFRL). An experiment conducted at a fixed observatory demonstrated the lidar-observed diel Chl-a concentration variations. The results showed that diel variations of Chl-a and the formation of subsurface phytoplankton layers were driven by light availability and variations in water temperature. Furthermore, the facilitation from solar radiation-regulated water temperature on the phytoplankton growth rate was revealed by the high correlation between water temperature and Chl-a concentration anomalies. Lidar technology is expected to provide new insights into continuous three-dimension observations and be of great importance in dynamic inland water ecosystems.

The lidar ratio of seawater is an essential quantity related to both lidar retrieval and water constituent. However, few studies discuss its spatial–temporal characteristics and ecological significance, which limits its applications in lidar remote sensing and marine science. This paper investigates the spatial–temporal characteristics and ecological significance of the lidar ratio of seawater using satellite passive remote sensing, which is validated by in situ measurements. Spatially, nearshore lidar ratio values are higher than offshore, mainly owing to the high concentration of colored dissolved organic matter in nearshore water. Temporally, the lidar ratio in each hemisphere exhibits lower values in summer than in winter due to the annual boom–bust cycle of phytoplankton. Furthermore, the variability patterns of the lidar ratio are nearly consistent with those of the chlorophyll-to-carbon ratio, implying the high ecological significance of phytoplankton physiology. These findings will provide the foundation for the application of lidar ratio in marine science and lidar remote sensing.