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Exploring the molecular foundation of the gene-regulatory systems underlying agronomic parameters or/and plant responses to both abiotic and biotic stresses is crucial for crop improvement. Thus, transcription factors, which alone or in combination directly regulated the targeted gene expression levels, are appropriate players for enlightening agronomic parameters through genetic engineering. In this regard, homeodomain leucine zipper (HD-ZIP) genes family concerned with enlightening plant growth and tolerance to environmental stresses are considered key players for crop improvement. This gene family containing HD and LZ domain belongs to the homeobox superfamily. It is further classified into four subfamilies, namely HD-ZIP I, HD-ZIP II, HD-ZIP III, and HD-ZIP IV. The first HD domain-containing gene was discovered in maize cells almost three decades ago. Since then, with advanced technologies, these genes were functionally characterized for their distinct roles in overall plant growth and development under adverse environmental conditions. This review summarized the different functions of HD-ZIP genes in plant growth and physiological-related activities from germination to fruit development. Additionally, the HD-ZIP genes also respond to various abiotic and biotic environmental stimuli by regulating defense response of plants. This review, therefore, highlighted the various significant aspects of this important gene family based on the recent findings. The practical application of HD-ZIP biomolecules in developing bioengineered plants will not only mitigate the negative effects of environmental stresses but also increase the overall production of crop plants.
Rahat Sharif; Ali Raza; Peng Chen; Yuhong Li; Enas M. El-Ballat; Abdur Rauf; Christophe Hano; Mohamed A. El-Esawi. HD-ZIP Gene Family: Potential Roles in Improving Plant Growth and Regulating Stress-Responsive Mechanisms in Plants. Genes 2021, 12, 1256 .
AMA StyleRahat Sharif, Ali Raza, Peng Chen, Yuhong Li, Enas M. El-Ballat, Abdur Rauf, Christophe Hano, Mohamed A. El-Esawi. HD-ZIP Gene Family: Potential Roles in Improving Plant Growth and Regulating Stress-Responsive Mechanisms in Plants. Genes. 2021; 12 (8):1256.
Chicago/Turabian StyleRahat Sharif; Ali Raza; Peng Chen; Yuhong Li; Enas M. El-Ballat; Abdur Rauf; Christophe Hano; Mohamed A. El-Esawi. 2021. "HD-ZIP Gene Family: Potential Roles in Improving Plant Growth and Regulating Stress-Responsive Mechanisms in Plants." Genes 12, no. 8: 1256.
The plant growth regulator forchlorfenuron is often applied to promote fruit setting and development in cucumber production. However, the effect of forchlorfenuron on the appearance and nutritional quality of cucumber is unknown. In the present study, forchlorfenuron was applied to female flowers 1 day before anthesis and at the day of anthesis. The application of forchlorfenuron successfully induced parthenocarpic fruit formation in cucumber. In addition, cytokinin-responsive genes were upregulated under forchlorfenuron treatment. Fruit treated with forchlorfenuron did not differ from pollinated fruit in shape, texture and major nutrients, such as protein, total flavonoids and vitamin C, with the exception of the lower phenolic acid content. Overall, our finding suggests that forchlorfenuron could be vital in promoting the parthenocarpic fruit set without altering the quality of cucumber.
Li Su; Miaoqing Wang; Yuean Wang; Rahat Sharif; Nannan Ren; Chunlu Qian; Jun Xu; Xuehao Chen; Xiaohua Qi. Forchlorfenuron Application Induced Parthenocarpic Fruit Formation without Affecting Fruit Quality of Cucumber. Horticulturae 2021, 7, 128 .
AMA StyleLi Su, Miaoqing Wang, Yuean Wang, Rahat Sharif, Nannan Ren, Chunlu Qian, Jun Xu, Xuehao Chen, Xiaohua Qi. Forchlorfenuron Application Induced Parthenocarpic Fruit Formation without Affecting Fruit Quality of Cucumber. Horticulturae. 2021; 7 (6):128.
Chicago/Turabian StyleLi Su; Miaoqing Wang; Yuean Wang; Rahat Sharif; Nannan Ren; Chunlu Qian; Jun Xu; Xuehao Chen; Xiaohua Qi. 2021. "Forchlorfenuron Application Induced Parthenocarpic Fruit Formation without Affecting Fruit Quality of Cucumber." Horticulturae 7, no. 6: 128.
TAZ (transcriptional coactivator with PDZ-binding) zinc finger domains, also known as transcription adaptor putative zinc finger domains, that control diverse function in plant growth and development. Here, in the present study, we evaluated the role of the TAZ domain-containing gene in response to various heavy metals. Initially, we found a total of 3, 7, 8, 9, 9, 9, 7, 14, 6, 10, and 6 proteins containing TAZ domain in stiff brome, millet, sorghum, potato, pepper, maize, rice, apple, peach, pear, and tomato genome that could trigger the plant resistance against various heavy metals, respectively. Various in-silico approaches were applied such as duplication, phylogenetic analysis, and gene structure, to understand the basic features of the TAZ domain-containing genes in plants. Gene expression analyses were also performed under heavy metals (Cr, Zn, Ni, Cd, Co, Fe, Mn, and Pb). The results of quantitative real-time PCR analysis indicated that the TAZ gene family members were differentially expressed under different heavy metals. We further characterized the functions of the TAZ domain-containing gene under the heavy metal stresses by overexpressing the OsTAZ4 gene in Arabidopsis. The TAZ genes could promote plant resistance against various heavy metals by interacting with OsMYB34 and OsFHA9 transcription factors. The results will contribute to elucidate the relationship of TAZ proteins with heavy metals stresses and also ascertain the biological function in plant growth and development.
Abdullah Shalmani; Uzair Ullah; Izhar Muhammad; Dong Zhang; Rahat Sharif; Peng Jia; Noor Saleem; Nazish Gul; Aizhan Rakhmanova; Muhammad Mobeen Tahir; Kun-Ming Chen; Na An. The TAZ domain-containing proteins play important role in the heavy metals stress biology in plants. Environmental Research 2021, 197, 111030 .
AMA StyleAbdullah Shalmani, Uzair Ullah, Izhar Muhammad, Dong Zhang, Rahat Sharif, Peng Jia, Noor Saleem, Nazish Gul, Aizhan Rakhmanova, Muhammad Mobeen Tahir, Kun-Ming Chen, Na An. The TAZ domain-containing proteins play important role in the heavy metals stress biology in plants. Environmental Research. 2021; 197 ():111030.
Chicago/Turabian StyleAbdullah Shalmani; Uzair Ullah; Izhar Muhammad; Dong Zhang; Rahat Sharif; Peng Jia; Noor Saleem; Nazish Gul; Aizhan Rakhmanova; Muhammad Mobeen Tahir; Kun-Ming Chen; Na An. 2021. "The TAZ domain-containing proteins play important role in the heavy metals stress biology in plants." Environmental Research 197, no. : 111030.
The effects of salt stress on the growth, nodulation, and nitrogen (N) fixation of legumes are well known, but the relationship between symbiotic nitrogen fixation (SNF) driven by rhizobium–legume symbiosis and salt tolerance in Medicago truncatula is not well studied. The effects of the active nodulation process on salt stress tolerance of Medicago truncatula were evaluated by quantifying the compatible solutes, soluble sugars, and antioxidants enzymes, as well as growth and survival rate of plants. Eight weeks old plants, divided in three groups: (i) no nodules (NN), (ii) inactive nodules (IN), and (iii) active nodules (AN), were exposed to 150 mM of NaCl salt stress for 0, 8, 16, 24, 32, 40, and 48 h in hydroponic system. AN plants showed a higher survival rate (30.83% and 38.35%), chlorophyll contents (37.18% and 44.51%), and photosynthesis compared to IN and NN plants, respectively. Improved salt tolerance in AN plants was linked with higher activities of enzymatic and nonenzymatic antioxidants and higher K+ (20.45% and 39.21%) and lower Na+ accumulations (17.54% and 24.51%) when compared with IN and NN plants, respectively. Additionally, higher generation of reactive oxygen species (ROS) was indicative of salt stress, causing membrane damage as revealed by higher electrolyte leakage and lipid peroxidation. All such effects were significantly ameliorated in AN plants, showing higher compatible solutes (proline, free amino acids, glycine betaine, soluble sugars, and proteins) and maintaining higher relative water contents (61.34%). This study advocates positive role of Rhizobium meliloti inoculation against salt stress through upregulation of antioxidant system and a higher concentration of compatible solutes.
Annie Irshad; Rana Rehman; Muhammad Abrar; Qudsia Saeed; Rahat Sharif; Tianming Hu. Contribution of Rhizobium–Legume Symbiosis in Salt Stress Tolerance in Medicago truncatula Evaluated through Photosynthesis, Antioxidant Enzymes, and Compatible Solutes Accumulation. Sustainability 2021, 13, 3369 .
AMA StyleAnnie Irshad, Rana Rehman, Muhammad Abrar, Qudsia Saeed, Rahat Sharif, Tianming Hu. Contribution of Rhizobium–Legume Symbiosis in Salt Stress Tolerance in Medicago truncatula Evaluated through Photosynthesis, Antioxidant Enzymes, and Compatible Solutes Accumulation. Sustainability. 2021; 13 (6):3369.
Chicago/Turabian StyleAnnie Irshad; Rana Rehman; Muhammad Abrar; Qudsia Saeed; Rahat Sharif; Tianming Hu. 2021. "Contribution of Rhizobium–Legume Symbiosis in Salt Stress Tolerance in Medicago truncatula Evaluated through Photosynthesis, Antioxidant Enzymes, and Compatible Solutes Accumulation." Sustainability 13, no. 6: 3369.
: The small YABBY plant-specific transcription factor has a prominent role in regulating plant growth and developmental activities. However, little information is available about YABBY gene family in Triticum aestivum L. Herein, we identified 21 TaYABBY genes in the Wheat genome database. Then, we performed the conserved motif and domain analysis of TaYABBY proteins. The phylogeny of the TaYABBY was further sub-divided into 6 subfamilies (YABBY1/YABBY3, YABB2, YABBY5, CRC and INO) based on the structural similarities and functional diversities. The GO (Gene ontology) analysis of TaYABBY proteins showed that they are involved in numerous developmental processes and showed response against environmental stresses. The analysis of all identified genes in RNA-seq data showed that they are expressed in different tissues of wheat. Differential expression patterns were observed in not only control samples but also in stressed samples such as biotic stress (i.e., Fusarium graminearum (F.g), septoria tritici (STB), Stripe rust (Sr) and Powdery mildew (Pm), and abiotic stress (i.e., drought, heat, combined drought and heat and phosphorus deficiency), especially at different grain development stages. All identified TaYABBY-genes were localized in the nucleus which implies their participation in the regulatory mechanisms of various biological and cellular processes. In light of the above-mentioned outcomes, it has been deduced that TaYABBY-genes in the wheat genome play an important role in mediating various development, growth, and resistance mechanism, which could provide significant clues for future functional studies.
Zeeshan Ali Buttar; Yuan Yang; Rahat Sharif; Sheng Nan Wu; Yanzhou Xie; Chengshe Wang. Genome Wide Identification, Characterization, and Expression Analysis of YABBY-Gene Family in WHEAT (Triticum aestivum L). Agronomy 2020, 10, 1189 .
AMA StyleZeeshan Ali Buttar, Yuan Yang, Rahat Sharif, Sheng Nan Wu, Yanzhou Xie, Chengshe Wang. Genome Wide Identification, Characterization, and Expression Analysis of YABBY-Gene Family in WHEAT (Triticum aestivum L). Agronomy. 2020; 10 (8):1189.
Chicago/Turabian StyleZeeshan Ali Buttar; Yuan Yang; Rahat Sharif; Sheng Nan Wu; Yanzhou Xie; Chengshe Wang. 2020. "Genome Wide Identification, Characterization, and Expression Analysis of YABBY-Gene Family in WHEAT (Triticum aestivum L)." Agronomy 10, no. 8: 1189.
Cadmium (Cd) is one of the most toxic metals in the environment, and has noxious effects on plant growth and production. Cd-accumulating plants showed reduced growth and productivity. Therefore, remediation of this non-essential and toxic pollutant is a prerequisite. Plant-based phytoremediation methodology is considered as one a secure, environmentally friendly, and cost-effective approach for toxic metal remediation. Phytoremediating plants transport and accumulate Cd inside their roots, shoots, leaves, and vacuoles. Phytoremediation of Cd-contaminated sites through hyperaccumulator plants proves a ground-breaking and profitable choice to combat the contaminants. Moreover, the efficiency of Cd phytoremediation and Cd bioavailability can be improved by using plant growth-promoting bacteria (PGPB). Emerging modern molecular technologies have augmented our insight into the metabolic processes involved in Cd tolerance in regular cultivated crops and hyperaccumulator plants. Plants’ development via genetic engineering tools, like enhanced metal uptake, metal transport, Cd accumulation, and the overall Cd tolerance, unlocks new directions for phytoremediation. In this review, we outline the physiological, biochemical, and molecular mechanisms involved in Cd phytoremediation. Further, a focus on the potential of omics and genetic engineering strategies has been documented for the efficient remediation of a Cd-contaminated environment.
Ali Raza; Madiha Habib; Shiva Najafi Kakavand; Zainab Zahid; Noreen Zahra; Rahat Sharif; Mirza Hasanuzzaman. Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms. Biology 2020, 9, 177 .
AMA StyleAli Raza, Madiha Habib, Shiva Najafi Kakavand, Zainab Zahid, Noreen Zahra, Rahat Sharif, Mirza Hasanuzzaman. Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms. Biology. 2020; 9 (7):177.
Chicago/Turabian StyleAli Raza; Madiha Habib; Shiva Najafi Kakavand; Zainab Zahid; Noreen Zahra; Rahat Sharif; Mirza Hasanuzzaman. 2020. "Phytoremediation of Cadmium: Physiological, Biochemical, and Molecular Mechanisms." Biology 9, no. 7: 177.
Sugarcane is an important crop from Poaceae family, contributing about 80% of the total world’s sucrose with an annual value of around US$150 billion. In addition, sugarcane is utilized as a raw material for the production of bioethanol, which is an alternate source of renewable energy. Moving towards sugarcane omics, a remarkable success has been achieved in gene transfer from a wide variety of plant and non-plant sources to sugarcane, with the accessibility of efficient transformation systems, selectable marker genes, and genetic engineering gears. Genetic engineering techniques make possible to clone and characterize useful genes and also to improve commercially important traits in elite sugarcane clones that subsequently lead to the development of an ideal cultivar. Sugarcane is a complex polyploidy crop, and hence no single technique has been found to be the best for the confirmation of polygenic and phenotypic characteristics. To better understand the application of basic omics in sugarcane regarding agronomic characters and industrial quality traits as well as responses to diverse biotic and abiotic stresses, it is important to explore the physiology, genome structure, functional integrity, and collinearity of sugarcane with other more or less similar crops/plants. Genetic improvements in this crop are hampered by its complex genome, low fertility ratio, longer production cycle, and susceptibility to several biotic and abiotic stresses. Biotechnology interventions are expected to pave the way for addressing these obstacles and improving sugarcane crop. Thus, this review article highlights up to date information with respect to how advanced data of omics (genomics, transcriptomic, proteomics and metabolomics) can be employed to improve sugarcane crops.
Ahmad Ali; Mehran Khan; Rahat Sharif; Muhammad Mujtaba; San-Ji Gao. Sugarcane Omics: An Update on the Current Status of Research and Crop Improvement. Plants 2019, 8, 344 .
AMA StyleAhmad Ali, Mehran Khan, Rahat Sharif, Muhammad Mujtaba, San-Ji Gao. Sugarcane Omics: An Update on the Current Status of Research and Crop Improvement. Plants. 2019; 8 (9):344.
Chicago/Turabian StyleAhmad Ali; Mehran Khan; Rahat Sharif; Muhammad Mujtaba; San-Ji Gao. 2019. "Sugarcane Omics: An Update on the Current Status of Research and Crop Improvement." Plants 8, no. 9: 344.
Malectin/malectin-like domain containing proteins are a novel identified protein subfamily of lectins that regulates various functional bioprocesses in plants. However, little is known about the phylogenetic relationships and functions of this gene family in rice. Here, in this study, a total of 84 malectin/malectin-like domain containing proteins, including 67 malectin/malectin-like receptor-like kinases (MRLKs) and 17 malectin/malectin-like receptor-like proteins (MRLPs), were identified in rice. These MRLK and MRLP proteins can be classified into four subfamilies based on the malectin/malectin-like domain present in their extracellular regions. The OsMRLK and OsMRLP gene family members are localized on all the chromosomes in rice genome with the members within the same subfamily sharing similar exon–intron distribution and motif composition. The multiple members and diverse gene and protein structures demonstrate that the malectin/malectin-like domain protein family underscores a functional diversity and divergence in rice. In addition, the expression levels of the OsMRLK gene family members are greatly varied between the tissues and organs at different developmental stages of rice. Furthermore, the majority of the tested OsMRLKs showed significant upregulation in transcripts under a number of abiotic stresses, hormonal applications, and heavy metals, suggesting the vital roles of the OsMRLK genes in rice development and multi-stress tolerance. The results obtained here provide a well fundament for further clarification of the roles of the malectin/malectin-like domain containing proteins in plants.
Xiu-Qing Jing; Abdullah Shalmani; Meng-Ru Zhou; Peng-Tao Shi; Izhar Muhammad; Yi Shi; Rahat Sharif; Wen-Qiang Li; Wen-Ting Liu; Kun-Ming Chen. Genome-Wide Identification of Malectin/Malectin-Like Domain Containing Protein Family Genes in Rice and Their Expression Regulation Under Various Hormones, Abiotic Stresses, and Heavy Metal Treatments. Journal of Plant Growth Regulation 2019, 39, 492 -506.
AMA StyleXiu-Qing Jing, Abdullah Shalmani, Meng-Ru Zhou, Peng-Tao Shi, Izhar Muhammad, Yi Shi, Rahat Sharif, Wen-Qiang Li, Wen-Ting Liu, Kun-Ming Chen. Genome-Wide Identification of Malectin/Malectin-Like Domain Containing Protein Family Genes in Rice and Their Expression Regulation Under Various Hormones, Abiotic Stresses, and Heavy Metal Treatments. Journal of Plant Growth Regulation. 2019; 39 (1):492-506.
Chicago/Turabian StyleXiu-Qing Jing; Abdullah Shalmani; Meng-Ru Zhou; Peng-Tao Shi; Izhar Muhammad; Yi Shi; Rahat Sharif; Wen-Qiang Li; Wen-Ting Liu; Kun-Ming Chen. 2019. "Genome-Wide Identification of Malectin/Malectin-Like Domain Containing Protein Family Genes in Rice and Their Expression Regulation Under Various Hormones, Abiotic Stresses, and Heavy Metal Treatments." Journal of Plant Growth Regulation 39, no. 1: 492-506.
Recently-developed molecular markers are becoming powerful tools, with applications in crop genetics and improvement. Microsatellites, or simple sequence repeats (SSRs), are widely used in genetic fingerprinting, kinship analysis, and population genetics, because of the advantages of high variability from co-dominant and multi-allelic polymorphisms, and accurate and rapid detection. However, more recent evidence suggests they may play an important role in genome evolution and provide hotspots of recombination. This review describes the development of SSR markers through different techniques, and the detection of SSR markers and applications for sugarcane genetic research and breeding, such as cultivar identification, genetic diversity, genome mapping, quantitative trait loci (QTL) analysis, paternity analysis, cross-species transferability, segregation analysis, phylogenetic relationships, and identification of wild cross hybrids. We also discuss the advantages and disadvantages of SSR markers and highlight some future perspectives.
Ali Ahmad; Jin-Da Wang; Yong-Bao Pan; Rahat Sharif; San-Ji Gao. Development and Use of Single Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies. Agronomy 2018, 8, 260 .
AMA StyleAli Ahmad, Jin-Da Wang, Yong-Bao Pan, Rahat Sharif, San-Ji Gao. Development and Use of Single Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies. Agronomy. 2018; 8 (11):260.
Chicago/Turabian StyleAli Ahmad; Jin-Da Wang; Yong-Bao Pan; Rahat Sharif; San-Ji Gao. 2018. "Development and Use of Single Sequence Repeats (SSRs) Markers for Sugarcane Breeding and Genetic Studies." Agronomy 8, no. 11: 260.
Melatonin (N-acetyl-5-methoxytryptamine) is a nontoxic biological molecule produced in a pineal gland of animals and different tissues of plants. It is an important secondary messenger molecule, playing a vital role in coping with various abiotic and biotic stresses. Melatonin serves as an antioxidant in postharvest technology and enhances the postharvest life of fruits and vegetables. The application of exogenous melatonin alleviated reactive oxygen species and cell damage induced by abiotic and biotic stresses by means of repairing mitochondria. Additionally, the regulation of stress-specific genes and the activation of pathogenesis-related protein and antioxidant enzymes genes under biotic and abiotic stress makes it a more versatile molecule. Besides that, the crosstalk with other phytohormones makes inroads to utilize melatonin against non-testified stress conditions, such as viruses and nematodes. Furthermore, different strategies have been discussed to induce endogenous melatonin activity in order to sustain a plant system. Our review highlighted the diverse roles of melatonin in a plant system, which could be useful in enhancing the environmental friendly crop production and ensure food safety.
Rahat Sharif; Chen Xie; Haiqiang Zhang; Marino B. Arnao; Muhammad Ali; Qasid Ali; Izhar Muhammad; Abdullah Shalmani; Muhammad Azher Nawaz; Peng Chen; Yuhong Li. Melatonin and Its Effects on Plant Systems. Molecules 2018, 23, 2352 .
AMA StyleRahat Sharif, Chen Xie, Haiqiang Zhang, Marino B. Arnao, Muhammad Ali, Qasid Ali, Izhar Muhammad, Abdullah Shalmani, Muhammad Azher Nawaz, Peng Chen, Yuhong Li. Melatonin and Its Effects on Plant Systems. Molecules. 2018; 23 (9):2352.
Chicago/Turabian StyleRahat Sharif; Chen Xie; Haiqiang Zhang; Marino B. Arnao; Muhammad Ali; Qasid Ali; Izhar Muhammad; Abdullah Shalmani; Muhammad Azher Nawaz; Peng Chen; Yuhong Li. 2018. "Melatonin and Its Effects on Plant Systems." Molecules 23, no. 9: 2352.
BBX proteins play important roles in regulating plant growth and development including photomorphogenesis, photoperiodic regulation of flowering, and responses to biotic and abiotic stresses. At present, the genomes of seven Rosacea fruit species have been fully sequenced. However, little is known about the BBX gene family and their evolutionary history in these Rosacea species. Therefore, in this study total, 212 BBX genes were investigated from seven Rosacea species (67 from Malus × domestica, 40 from Pyruscommunis, 22 from Rosa Chinesis, 20 from Prunuspersica, 21 from Fragariavesca, 22 from Prunusavium, and 20 from Rubusoccidentalis). The chemical properties, gene structures, and evolutionary relationships of the BBX genes were also studied. All the BBX genes were grouped into six subfamilies on the basis of their phylogenetic relationships and structural features. Analysis of gene structure, segmental and tandem duplication, gene phylogeny, and tissue-specific expression with the ArrayExpress database showed their diversification in function, quantity, and structure. The expression profiles of 19 MdBBX genes in different tissues were evaluated through qRT-PCR. These genes showed distinct transcription level among the tested tissues (bud, flower, fruit, stem, and leaf). Moreover, expression patterns of 19 MdBBX genes were examined during flowering induction time under flowering-related hormones and treatments (GA3, 6-BA, and sucrose). The expressions of the candidates BBX genes were affected and showed diverse expression profile. Furthermore, changes in response to these flowering-related hormones and treatment specifying their potential involvement in flowering induction. Based on these findings, BBX genes could be used as potential genetic markers for the growth and development of plants particularly in the area of functional analysis, and their involvement in flower induction in fruit plants.
Abdullah Shalmani; Sheng Fan; Peng Jia; Guofang Li; Izhar Muhammad; Youmei Li; Rahat Sharif; Feng Dong; Xiya Zuo; Ke Li; Kun-Ming Chen; Mingyu Han. Genome Identification of B-BOX Gene Family Members in Seven Rosacea Species and Their Expression Analysis in Response to Flower Induction in Malus domestica. Molecules 2018, 23, 1763 .
AMA StyleAbdullah Shalmani, Sheng Fan, Peng Jia, Guofang Li, Izhar Muhammad, Youmei Li, Rahat Sharif, Feng Dong, Xiya Zuo, Ke Li, Kun-Ming Chen, Mingyu Han. Genome Identification of B-BOX Gene Family Members in Seven Rosacea Species and Their Expression Analysis in Response to Flower Induction in Malus domestica. Molecules. 2018; 23 (7):1763.
Chicago/Turabian StyleAbdullah Shalmani; Sheng Fan; Peng Jia; Guofang Li; Izhar Muhammad; Youmei Li; Rahat Sharif; Feng Dong; Xiya Zuo; Ke Li; Kun-Ming Chen; Mingyu Han. 2018. "Genome Identification of B-BOX Gene Family Members in Seven Rosacea Species and Their Expression Analysis in Response to Flower Induction in Malus domestica." Molecules 23, no. 7: 1763.
Chitosan is a naturally occurring compound and is commercially produced from seafood shells. It has been utilized in the induction of the defense system in both pre and post-harvest fruits and vegetables against fungi, bacteria, viruses, and other abiotic stresses. In addition to that, chitosan effectively improves the physiological properties of plants and also enhances the shelf life of post-harvest produces. Moreover, chitosan treatment regulates several genes in plants, particularly the activation of plant defense signaling pathways. That includes the elicitation of phytoalexins and pathogenesis-related (PR) protein. Besides that, chitosan has been employed in soil as a plant nutrient and has shown great efficacy in combination with other industrial fertilizers without affecting the soil’s beneficial microbes. Furthermore, it is helpful in reducing the fertilizer losses due to its coating ability, which is important in keeping the environmental pollution under check. Based on exhibiting such excellent properties, there is a striking interest in using chitosan biopolymers in agriculture systems. Therefore, our current review has been centered upon the multiple roles of chitosan in horticultural crops that could be useful in future crop improvement programs.
Rahat Sharif; Muhammad Mujtaba; Mati Ur Rahman; Abdullah Shalmani; Husain Ahmad; Toheed Anwar; Deng Tianchan; Xiping Wang. The Multifunctional Role of Chitosan in Horticultural Crops; A Review. Molecules 2018, 23, 872 .
AMA StyleRahat Sharif, Muhammad Mujtaba, Mati Ur Rahman, Abdullah Shalmani, Husain Ahmad, Toheed Anwar, Deng Tianchan, Xiping Wang. The Multifunctional Role of Chitosan in Horticultural Crops; A Review. Molecules. 2018; 23 (4):872.
Chicago/Turabian StyleRahat Sharif; Muhammad Mujtaba; Mati Ur Rahman; Abdullah Shalmani; Husain Ahmad; Toheed Anwar; Deng Tianchan; Xiping Wang. 2018. "The Multifunctional Role of Chitosan in Horticultural Crops; A Review." Molecules 23, no. 4: 872.
Endonuclease I is a widely distributed periplasmic or extracellular enzyme. A method for the high-level production of recombinant AsEndI (endonuclease I from Aliivibrio salmonicida) in Escherichia coli with secretion expression is investigated. The coding sequence of AsEndI gene was assembled according to the E. coli codon usage bias, and AsEndI was expressed in the periplasm of E. coli TOP10 with a C-terminal 6× His-tagged fusion. The recombinant AsEndI (His-AsEndI) was purified by Ni-NTA resin with a yield of 1.29 × 107 U from 1-L LB medium. His-AsEndI could be classified into Ca2+/Mg2+-dependent nucleases and showed highest nuclease activity to dsDNA at pH 8.0 and 37 °C. His-AsEndI is highly active in a broad range of salt concentration range up to 1.0 M with optimal NaCl concentration at 0.4 M. His-AsEndI can effectively remove DNA contamination in RNA sample or in PCR reagents to the level that cannot be detected by highly sensitive nested PCR and without adverse effects on the subsequent PCR reaction. His-AsEndI can remove DNA contamination at high salt conditions, especially for the DNA that may be shielded by DNA-binding protein at low salt conditions.
Yuan Wang; Peng Jia; Rahat Sharif; Zhengchen Li; Yuhong Li; Li ZhengChen. High-Level Production of DNA-Specific Endonuclease AsEndI with Synonymous Codon and its Potential Utilization for Removing DNA Contamination. Applied Biochemistry and Biotechnology 2017, 185, 641 -654.
AMA StyleYuan Wang, Peng Jia, Rahat Sharif, Zhengchen Li, Yuhong Li, Li ZhengChen. High-Level Production of DNA-Specific Endonuclease AsEndI with Synonymous Codon and its Potential Utilization for Removing DNA Contamination. Applied Biochemistry and Biotechnology. 2017; 185 (3):641-654.
Chicago/Turabian StyleYuan Wang; Peng Jia; Rahat Sharif; Zhengchen Li; Yuhong Li; Li ZhengChen. 2017. "High-Level Production of DNA-Specific Endonuclease AsEndI with Synonymous Codon and its Potential Utilization for Removing DNA Contamination." Applied Biochemistry and Biotechnology 185, no. 3: 641-654.