The land crab Johngarthia lagostoma is endemic to Trindade Island, Atol das Rocas, Fernando de Noronha and Ascension Islands. The natural history of the species in non-breeding periods is little known. Therefore, here we reported the formation of non-breeding groups and evaluated the chromatic populational pattern of J. lagostoma in Trindade Island. Records were obtained between April and June 2015. The groups were characterized according to their location, terrain elevation, environmental characteristics and specimens’ behavior. The chromatic pattern was defined by the classification of individuals between yellow and purple, and the proportion of each color was compared between populational units (previously defined based on genetic differences). Non-breeding groups were recorded in four locations in Trindade Island, at altitudes < 40 m, and all of them were in locations with food resources and sediment suitable for the construction of shelters. Isolated individuals or the absence of the species were observed in the most inhospitable places, indicating that the maintenance of the species depends on portions of suitable habitat amid the currently arid matrix. Yellow individuals (96.4%) were predominant on Trindade Island and the chromatic pattern differed from the other populations. Color patterns seem to follow genetic differences between populations, and the founder effect may account for current patterns. From the data obtained, we emphasize that the maintenance of the species may depend on food, sediment suitable for shelters construction, humidity and shade. Due to the significant population decline in other regions, the need to define guidelines for the conservation of the species on Trindade Island is highlighted. In this context, the regeneration of insular vegetation and prohibiting the known anthropic consumption of individuals may represent important strategies for the maintenance of the species.

Roadkill is one of the main threats associated with highways, directly contributing to the depletion of natural populations. Here we establish the effect of landscape, seasonality and climatic variables on roadkill of wild vertebrates on two highways associated with Pedra Azul State Park (PEPAZ), state of Espírito Santo, southeastern Brazil. The highways BR-262 and ES-164 (20 km stretch in each road) were sampled monthly, between January and December 2015, totaling 60 days sampled/stretch. A total of 355 roadkilled vertebrates (Amphibia = 10; Reptilia = 40; Aves = 155; Mammalia = 149; undetermined = 1) distributed among at least 82 taxa were recorded (Amphibia = 4; Reptilia = 11; Aves = 47; Mammalia = 20). The roadkill rate was equivalent between highways, but birds were the most affected group on the ES-164 and mammals on the BR-262. A greater number of records were obtained during the rainy season for both highways. Temperature had a positive influence on the monthly roadkill rate, but no influence was observed from precipitation. The distance from water bodies and the type of land use and occupation influenced the roadkill records distribution. Roadkill of mammals was associated with areas of overgrown vegetation; birds with agricultural crops and built-up areas; and reptiles marginally with pasture areas. The effect of the highways was complementary because they affected the vertebrate classes differently. This could have severe impacts on biodiversity, highlighting that PEPAZ is part of an important ecological corridor of the Atlantic Forest in Espírito Santo. Speed control and installation of warning signs and wildlife crossing mechanisms are recommended to promote the long-term conservation of the regional biota.

Wild felids are vital to maintaining the ecological balance in natural environments as they regulate prey populations at different levels of the food chain. Changes in the dynamics of predator populations can impact the entire biodiversity of an ecosystem. There are few reports of Adenovirus infections in these animals, and little is known about their epidemiology. Therefore, a deeper understanding of these viruses within a One Health framework is essential, given their importance to animal, human, and environmental health. This study aimed to detect Adenovirus DNA in fecal samples of wild felids from a remnant of the Atlantic Forest in southeastern Brazil, renowned for its high biodiversity. A total of 43 fecal samples, 11 from jaguar (Panthera onca) and 32 from ocelot (Leopardus pardalis), were collected. The samples were subjected to viral nucleic acid extraction and genetic material amplification through PCR, followed by nucleotide sequencing. All phylogenetic analyses were based on the amino acid sequences of the DNA polymerase and IV2a genes. Adenovirus DNA was detected in the feces of both species, with two samples of each feline testing positive. This study reports, for the first time, the occurrence of Adenovirus associated with feces of Panthera onca and Leopardus pardalis. All detected sequences were grouped within the Mastadenovirus genus. Based solely on phylogenetic distance criteria, the identified sequences could be classified as Mastadenovirus bosprimum and Mastadenovirus from the vampire bat Desmodus rotundus. We hypothesize that Adenoviruses were associated with the prey consumed, which may allow the felines to act as eventual viral dispersing agents in the environment, in addition to the risk of being infected. This study provides new information on the association of Adenoviruses with wild felids and their prey, and offers important insights into the ecological dynamics of these viruses in natural environments. It suggests that wild felines may play a crucial role in viral surveillance programs.

The coexistence of functionally similar predators offers a framework for understanding the mechanisms shaping ecological communities. Jaguars and pumas are broadly sympatric in the Neotropics, yet the processes sustaining their persistence remain unclear. Classical niche theory predicts that coexistence requires ecological differentiation, whereas modern models emphasize balancing stabilizing (reducing interspecific competition) and equalizing mechanisms (minimizing fitness differences). Although demographic components were not directly estimated, we integrated secondary dietary data compiled from 21 sympatric populations through a systematic literature review. This integration allowed us to identify trophic patterns consistent with coexistence mechanisms across taxonomic and functional prey axes. Analyses revealed strong taxonomic segregation at finer scales, with jaguars primarily consuming Artiodactyla, while pumas exploited a broader spectrum including Rodentia, Cingulata, and Pilosa. This divergence reduces direct competition, consistent with stabilizing resource partitioning. In contrast, high overlap in functional traits (e.g., body mass and locomotor habit) indicated functional convergence potentially increasing fitness equivalence, consistent with equalizing mechanisms. These dynamics suggested that jaguar–puma macroecological coexistence may be maintained by a dynamic balance between patterns consistent with stabilizing and equalizing mechanisms expressed within trophic niche axes. From a conservation perspective, strategies should move beyond species-specific approaches; preserving both taxonomic and functional prey diversity is essential to sustain the trophic requirements of predators and the ecosystems they regulate.