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Bifidobacterium longum is one of the most widely distributed and abundant Bifidobacterium in the human intestine, and has been proven to have a variety of physiological functions. In this study, 80 strains of B. longum isolated from human subjects were classified into subspecies by ANI and phylogenetic analyses, and the functional genes were compared. The results showed that there were significant differences in carbohydrate metabolism between the two subspecies, which determined their preference for human milk oligosaccharides or plant-derived carbohydrates. The predicted exopolysaccharide (EPS) gene clusters had large variability within species but without difference at the subspecies level. Four subtype CRISPR-Cas systems presented in B. longum, while the subtypes I-U and II-C only existed in B. longum subsp. longum. The bacteriocin operons in B. longum subsp. infantis were more widely distributed compared with B. longum subsp. longum. In conclusion, this study revealed the similarities and differences between B. longum subsp. infantis and B. longum subsp. longum, which could provide a theoretical basis for further exploring the probiotic characteristics of B. longum.
Mingjie Li; Xingya Zhou; Catherine Stanton; R. Paul Ross; Jianxin Zhao; Hao Zhang; Bo Yang; Wei Chen. Comparative Genomics Analyses Reveal the Differences between B. longum subsp. infantis and B. longum subsp. longum in Carbohydrate Utilisation, CRISPR-Cas Systems and Bacteriocin Operons. Microorganisms 2021, 9, 1713 .
AMA StyleMingjie Li, Xingya Zhou, Catherine Stanton, R. Paul Ross, Jianxin Zhao, Hao Zhang, Bo Yang, Wei Chen. Comparative Genomics Analyses Reveal the Differences between B. longum subsp. infantis and B. longum subsp. longum in Carbohydrate Utilisation, CRISPR-Cas Systems and Bacteriocin Operons. Microorganisms. 2021; 9 (8):1713.
Chicago/Turabian StyleMingjie Li; Xingya Zhou; Catherine Stanton; R. Paul Ross; Jianxin Zhao; Hao Zhang; Bo Yang; Wei Chen. 2021. "Comparative Genomics Analyses Reveal the Differences between B. longum subsp. infantis and B. longum subsp. longum in Carbohydrate Utilisation, CRISPR-Cas Systems and Bacteriocin Operons." Microorganisms 9, no. 8: 1713.
Increasing attention has been paid to the potential probiotic effects of Latilactobacillus sakei. To explore the genetic diversity of L. sakei, 14 strains isolated from different niches (feces, fermented kimchi, and meat products) and 54 published strains were compared and analyzed. The results showed that the average genome size and GC content of L.sakei were 1.98 Mb and 41.22%, respectively. Its core genome mainly encodes translation and transcription, amino acid synthesis, glucose metabolism, and defense functions. L.sakei has open pan-genomic characteristics, and its pan-gene curve shows an upward trend. The genetic diversity of L.sakei is mainly reflected in carbohydrate utilization, antibiotic tolerance, and immune/competition-related factors, such as clustering regular interval short palindromic repeat sequence (CRISPR)–Cas. The CRISPR system is mainly IIA type, and a few are IIC types. This work provides a basis for the study of this species.
Ying Chen; Nan Li; Shenxi Zhao; Chuan Zhang; Nanzhen Qiao; Hui Duan; Yue Xiao; Bowen Yan; Jianxin Zhao; Fengwei Tian; Qixiao Zhai; Leilei Yu; Wei Chen. Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches. Foods 2021, 10, 1717 .
AMA StyleYing Chen, Nan Li, Shenxi Zhao, Chuan Zhang, Nanzhen Qiao, Hui Duan, Yue Xiao, Bowen Yan, Jianxin Zhao, Fengwei Tian, Qixiao Zhai, Leilei Yu, Wei Chen. Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches. Foods. 2021; 10 (8):1717.
Chicago/Turabian StyleYing Chen; Nan Li; Shenxi Zhao; Chuan Zhang; Nanzhen Qiao; Hui Duan; Yue Xiao; Bowen Yan; Jianxin Zhao; Fengwei Tian; Qixiao Zhai; Leilei Yu; Wei Chen. 2021. "Integrated Phenotypic–Genotypic Analysis of Latilactobacillus sakei from Different Niches." Foods 10, no. 8: 1717.
Accumulating evidence from animal studies supports the potential role of probiotics and prebiotics in alleviating neurodegenerative diseases. However, whether dietary supplementation with probiotics improves cognitive function in patients with Alzheimer’s disease (AD) or mild cognitive impairment (MCI) is unclear. We searched literature databases for relevant randomized control trials and compared the outcomes between control/placebo and intervention groups. The results of the included studies were meta-analyzed using a random-effects model, with standardized mean differences (SMDs) and 95% confidence intervals (CIs) calculated as summary statistics. We also performed a risk-of-bias assessment, sensitivity analysis and subgroup analysis. Among the 294 articles identified, eight articles involving 174 patients with AD and 446 with MCI were included in the qualitative synthesis and seven studies were meta-analyzed. Our analysis detected high between-group heterogeneity (SMD = 0.43, 95% CI −0.02–0.88, p< 0.0001, I2 = 86.4%) in cognitive function across the included studies. Subgroup analyses identified a significant effect of probiotics on cognitive function only in the studies involving people with MCI (I2 = 44%, p = 0.15 for heterogeneity, p = 0.0002 for overall effect). Our findings suggest that dietary supplementation with probiotics improves cognitive function, especially in people with MCI.
Guangsu Zhu; Jianxin Zhao; Hao Zhang; Wei Chen; Gang Wang. Probiotics for Mild Cognitive Impairment and Alzheimer’s Disease: A Systematic Review and Meta-Analysis. Foods 2021, 10, 1672 .
AMA StyleGuangsu Zhu, Jianxin Zhao, Hao Zhang, Wei Chen, Gang Wang. Probiotics for Mild Cognitive Impairment and Alzheimer’s Disease: A Systematic Review and Meta-Analysis. Foods. 2021; 10 (7):1672.
Chicago/Turabian StyleGuangsu Zhu; Jianxin Zhao; Hao Zhang; Wei Chen; Gang Wang. 2021. "Probiotics for Mild Cognitive Impairment and Alzheimer’s Disease: A Systematic Review and Meta-Analysis." Foods 10, no. 7: 1672.
The oleaginous fungus Mortierella alpina has distinct advantages in long-chain PUFAs production, and it is the only source for dietary arachidonic acid (ARA) certificated by FDA and European Commission. This review provides an overall introduction to M. alpina, including its major research methods, key factors governing lipid biosynthesis, metabolic engineering and omics studies. Currently, the research interests in M. alpina focus on improving lipid yield and fatty acid desaturation degree by enhancing fatty acid precursors and the reducing power NADPH, and genetic manipulation on PUFAs synthetic pathways is carried to optimise fatty acid composition. Besides, multi-omics studies have been applied to elucidate the global regulatory mechanism of lipogenesis in M. alpina. However, research challenges towards achieving a lipid cell factory lie in strain breeding and cost control due to the coenocytic mycelium, long fermentation period and insufficient conversion rate from carbon to lipid. We also proposed future research goals based on a multilevel regulating strategy: obtaining ideal chassis by directional evolution and high-throughput screening; rewiring central carbon metabolism and inhibiting competitive pathways by multi-gene manipulation system to enhance carbon to lipid conversion rate; optimisation of protein function based on post-translational modification; application of dynamic fermentation strategies suitable for different fermentation phases. By reviewing the comprehensive research progress of this oleaginous fungus, we aim to further comprehend the fungal lipid metabolism and provide reference information and guidelines for the exploration of microbial oils from the perspectives of fundamental research to industrial application.
Lulu Chang; Hengqian Lu; Haiqin Chen; Xin Tang; Jianxin Zhao; Hao Zhang; Yong Q. Chen; Wei Chen. Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects. Biotechnology Advances 2021, 107794 .
AMA StyleLulu Chang, Hengqian Lu, Haiqin Chen, Xin Tang, Jianxin Zhao, Hao Zhang, Yong Q. Chen, Wei Chen. Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects. Biotechnology Advances. 2021; ():107794.
Chicago/Turabian StyleLulu Chang; Hengqian Lu; Haiqin Chen; Xin Tang; Jianxin Zhao; Hao Zhang; Yong Q. Chen; Wei Chen. 2021. "Lipid metabolism research in oleaginous fungus Mortierella alpina: Current progress and future prospects." Biotechnology Advances , no. : 107794.
Shenyan Ma; Jiang Cao; Ruolan Liliu; Nan Li; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. Effects of Bacillus coagulans as an adjunct starter culture on yogurt quality and storage. Journal of Dairy Science 2021, 104, 7466 -7479.
AMA StyleShenyan Ma, Jiang Cao, Ruolan Liliu, Nan Li, Jianxin Zhao, Hao Zhang, Wei Chen, Qixiao Zhai. Effects of Bacillus coagulans as an adjunct starter culture on yogurt quality and storage. Journal of Dairy Science. 2021; 104 (7):7466-7479.
Chicago/Turabian StyleShenyan Ma; Jiang Cao; Ruolan Liliu; Nan Li; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. 2021. "Effects of Bacillus coagulans as an adjunct starter culture on yogurt quality and storage." Journal of Dairy Science 104, no. 7: 7466-7479.
The role of supplementation with different Bacteroides fragilis (B. fragilis) strains in alleviating ulcerative colitis (UC) is unclear due to the controversial results from animal experiments.
Chen Wang; Sijia Li; Kan Hong; Leilei Yu; Fengwei Tian; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. The roles of different Bacteroides fragilis strains in protecting against DSS-induced ulcerative colitis and related functional genes. Food & Function 2021, 1 .
AMA StyleChen Wang, Sijia Li, Kan Hong, Leilei Yu, Fengwei Tian, Jianxin Zhao, Hao Zhang, Wei Chen, Qixiao Zhai. The roles of different Bacteroides fragilis strains in protecting against DSS-induced ulcerative colitis and related functional genes. Food & Function. 2021; ():1.
Chicago/Turabian StyleChen Wang; Sijia Li; Kan Hong; Leilei Yu; Fengwei Tian; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. 2021. "The roles of different Bacteroides fragilis strains in protecting against DSS-induced ulcerative colitis and related functional genes." Food & Function , no. : 1.
Psoriasis is an immune-mediated systemic disease that may be treated with probiotics. In this study, probiotic strains that could or could not decrease interleukin (IL)-17 levels were applied to imiquimod (IMQ)-induced psoriasis-like mice via oral administration. Bifidobacteriumadolescentis CCFM667, B. breve CCFM1078, Lacticaseibacillusparacasei CCFM1074, and Limosilactobacillus reuteri CCFM1132 ameliorated psoriasis-like pathological characteristics and suppressed the release of IL-23/T helper cell 17 (Th17) axis-related inflammatory cytokines, whereas B. animalis CCFM1148, L. paracasei CCFM1147, and L. reuteri CCFM1040 neither alleviated the pathological characteristics nor reduced the levels of inflammatory cytokines. All effective strains increased the contents of short-chain fatty acids, which were negatively correlated with the levels of inflammatory cytokines. By performing 16S rRNA gene sequencing, the diversity of gut microbiota in psoriasis-like mice was found to decrease, but all effective strains made some specific changes to the composition of gut microbiota compared to the ineffective strains. Furthermore, except for B. breve CCFM1078, all other effective strains decreased the abundance of the family Rikenellaceae, which was positively correlated with psoriasis-like pathological characteristics and was negatively correlated with propionate levels. These findings demonstrated effects of strain-specificity, and how probiotics ameliorated psoriasis and provide new possibilities for the treatment of psoriasis.
Wenwei Lu; Yadan Deng; Zhifeng Fang; Qixiao Zhai; Shumao Cui; Jianxin Zhao; Wei Chen; Hao Zhang. Potential Role of Probiotics in Ameliorating Psoriasis by Modulating Gut Microbiota in Imiquimod-Induced Psoriasis-Like Mice. Nutrients 2021, 13, 2010 .
AMA StyleWenwei Lu, Yadan Deng, Zhifeng Fang, Qixiao Zhai, Shumao Cui, Jianxin Zhao, Wei Chen, Hao Zhang. Potential Role of Probiotics in Ameliorating Psoriasis by Modulating Gut Microbiota in Imiquimod-Induced Psoriasis-Like Mice. Nutrients. 2021; 13 (6):2010.
Chicago/Turabian StyleWenwei Lu; Yadan Deng; Zhifeng Fang; Qixiao Zhai; Shumao Cui; Jianxin Zhao; Wei Chen; Hao Zhang. 2021. "Potential Role of Probiotics in Ameliorating Psoriasis by Modulating Gut Microbiota in Imiquimod-Induced Psoriasis-Like Mice." Nutrients 13, no. 6: 2010.
Lactobacillus ruminis can stimulate the immune response in vitro, but previous studies were only carried out in vitro and the anti-inflammatory effects of L. ruminis needs more in vivo evidences. In this study, the immune regulation and potential mechanisms of L. ruminis was investigated in DSS-induced colitis mice. L. ruminis FXJWS27L3 and L. ruminis FXJSW17L1 relieved the symptoms of colitis, including inhibition of colon shortening and colon tissue damage. L. ruminis FXJWS27L3 significantly reduced the pro-inflammatory cytokines IL-1β, TNF-α, and IL-17, while L. ruminis FXJSW17L1 significantly increased short chain fatty acids in mice feces. Moreover, L. ruminis FXJWS27L3 and L. ruminis FXJSW17L1 treatments significantly increased the gut microbiota diversity and balance the intestine microbiota profiles, which improved the imbalance of intestine microbiota composition to a certain extent. The results showed that L. ruminis can alleviate DSS-induced colitis, which possibly was related to promoting the expression of pro-inflammatory cytokines, up-regulating SCFAs and restoring the imbalance of gut microbiota.
Bo Yang; Mingjie Li; Shuo Wang; R. Ross; Catherine Stanton; Jianxin Zhao; Hao Zhang; Wei Chen. Lactobacillus ruminis Alleviates DSS-Induced Colitis by Inflammatory Cytokines and Gut Microbiota Modulation. Foods 2021, 10, 1349 .
AMA StyleBo Yang, Mingjie Li, Shuo Wang, R. Ross, Catherine Stanton, Jianxin Zhao, Hao Zhang, Wei Chen. Lactobacillus ruminis Alleviates DSS-Induced Colitis by Inflammatory Cytokines and Gut Microbiota Modulation. Foods. 2021; 10 (6):1349.
Chicago/Turabian StyleBo Yang; Mingjie Li; Shuo Wang; R. Ross; Catherine Stanton; Jianxin Zhao; Hao Zhang; Wei Chen. 2021. "Lactobacillus ruminis Alleviates DSS-Induced Colitis by Inflammatory Cytokines and Gut Microbiota Modulation." Foods 10, no. 6: 1349.
L. rhamnosus R31, L. rhamnosus R28-1 and L. reuteri L20M3 relieved hyperuricaemia in mouse model by promoting SCFA production.
Caixin Ni; Xin Li; Linlin Wang; Xiu Li; Jianxin Zhao; Hao Zhang; Gang Wang; Wei Chen. Lactic acid bacteria strains relieve hyperuricaemia by suppressing xanthine oxidase activity via a short-chain fatty acid-dependent mechanism. Food & Function 2021, 1 .
AMA StyleCaixin Ni, Xin Li, Linlin Wang, Xiu Li, Jianxin Zhao, Hao Zhang, Gang Wang, Wei Chen. Lactic acid bacteria strains relieve hyperuricaemia by suppressing xanthine oxidase activity via a short-chain fatty acid-dependent mechanism. Food & Function. 2021; ():1.
Chicago/Turabian StyleCaixin Ni; Xin Li; Linlin Wang; Xiu Li; Jianxin Zhao; Hao Zhang; Gang Wang; Wei Chen. 2021. "Lactic acid bacteria strains relieve hyperuricaemia by suppressing xanthine oxidase activity via a short-chain fatty acid-dependent mechanism." Food & Function , no. : 1.
We adopted a bioinformatics-based technique to identify strain-specific markers, which were then used to quantify the abundances of three distinct B. longum sup. longum strains in fecal samples of humans and mice. A pangenome analysis of 205 B. longum sup. longum genomes revealed the accumulation of considerable strain-specific genes within this species; specifically, 28.7% of the total identified genes were strain-specific. We identified 32, 14, and 49 genes specific to B. longum sup. longum RG4-1, B. longum sup. longum M1-20-R01-3, and B. longum sup. longum FGSZY6M4, respectively. After performing an in silico validation of these strain-specific markers using a nucleotide BLAST against both the B. longum sup. longum genome database and an NR/NT database, RG4-1_01874 (1331 bp), M1-20-R01-3_00324 (1745 bp), and FGSZY6M4_01477 (1691 bp) were chosen as target genes for strain-specific quantification. The specificities of the qPCR primers were validated against 47 non-target microorganisms and fecal baseline microbiota to ensure that they produced no PCR amplification products. The performance of the qPCR primer-based analysis was further assessed using fecal samples. After oral administration, the target B. longum strains appeared to efficiently colonize both the human and mouse guts, with average population levels of >108 CFU/g feces. The bioinformatics pipeline proposed here can be applied to the quantification of various bacterial species.
Yue Xiao; Chen Wang; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. Quantitative Detection of Bifidobacterium longum Strains in Feces Using Strain-Specific Primers. Microorganisms 2021, 9, 1159 .
AMA StyleYue Xiao, Chen Wang, Jianxin Zhao, Hao Zhang, Wei Chen, Qixiao Zhai. Quantitative Detection of Bifidobacterium longum Strains in Feces Using Strain-Specific Primers. Microorganisms. 2021; 9 (6):1159.
Chicago/Turabian StyleYue Xiao; Chen Wang; Jianxin Zhao; Hao Zhang; Wei Chen; Qixiao Zhai. 2021. "Quantitative Detection of Bifidobacterium longum Strains in Feces Using Strain-Specific Primers." Microorganisms 9, no. 6: 1159.
The decrease in ovarian hormone secretion that occurs during menopause results in an increase in body weight and adipose tissue mass. Probiotics and soy isoflavones (SIFs) could affect the gut microbiota and exert anti-obesity effects. The objective of this study was to investigate the effects of probiotics and a diet containing SIF (SIF diet) on ovariectomized mice with menopausal obesity, including the gut microbiome. The results demonstrate that Bifidobacterium longum 15M1 can reverse menopausal obesity, whilst the combination of Lactobacillus plantarum 30M5 and a SIF diet was more effective in alleviating menopausal lipid metabolism disorder than either components alone. Probiotics and SIFs play different anti-obesity roles in menopausal mice. Furthermore, 30M5 alters the metabolites of the gut microbiota that increase the circulating estrogen level, upregulates the expression of estrogen receptor α in abdominal adipose tissue and improves the production of short-chain fatty acids (SCFAs). A SIF diet can significantly alter the structure of the fecal bacterial community and enrich the pathways related to SCFAs production. Moreover, 30M5 and a SIF diet acted synergistically to effectively resolve abnormal serum lipid levels in ovariectomized mice, and these effects appear to be associated with regulation of the diversity and structure of the intestinal microbiota to enhance SCFAs production and promote estrogen circulation.
Qian Chen; Botao Wang; Shunhe Wang; Xin Qian; Xiu Li; Jianxin Zhao; Hao Zhang; Wei Chen; Gang Wang. Modulation of the Gut Microbiota Structure with Probiotics and Isoflavone Alleviates Metabolic Disorder in Ovariectomized Mice. Nutrients 2021, 13, 1793 .
AMA StyleQian Chen, Botao Wang, Shunhe Wang, Xin Qian, Xiu Li, Jianxin Zhao, Hao Zhang, Wei Chen, Gang Wang. Modulation of the Gut Microbiota Structure with Probiotics and Isoflavone Alleviates Metabolic Disorder in Ovariectomized Mice. Nutrients. 2021; 13 (6):1793.
Chicago/Turabian StyleQian Chen; Botao Wang; Shunhe Wang; Xin Qian; Xiu Li; Jianxin Zhao; Hao Zhang; Wei Chen; Gang Wang. 2021. "Modulation of the Gut Microbiota Structure with Probiotics and Isoflavone Alleviates Metabolic Disorder in Ovariectomized Mice." Nutrients 13, no. 6: 1793.
B. bifidum had intraspecies differences in relieving constipation. It can promote intestinal peristalsis by gastrointestinal active peptides. Specially, FXJCJ32M2 may promote the expression of AQP8 and VIPR1 through gut microbiota modulation.
Mao Chai; Linlin Wang; Xinping Li; Jianxin Zhao; Hao Zhang; Gang Wang; Wei Chen. Different Bifidobacterium bifidum strains change the intestinal flora composition of mice via different mechanisms to alleviate loperamide-induced constipation. Food & Function 2021, 1 .
AMA StyleMao Chai, Linlin Wang, Xinping Li, Jianxin Zhao, Hao Zhang, Gang Wang, Wei Chen. Different Bifidobacterium bifidum strains change the intestinal flora composition of mice via different mechanisms to alleviate loperamide-induced constipation. Food & Function. 2021; ():1.
Chicago/Turabian StyleMao Chai; Linlin Wang; Xinping Li; Jianxin Zhao; Hao Zhang; Gang Wang; Wei Chen. 2021. "Different Bifidobacterium bifidum strains change the intestinal flora composition of mice via different mechanisms to alleviate loperamide-induced constipation." Food & Function , no. : 1.
Enterotoxigenic Escherichia coli (ETEC) is the main bacterial cause of diarrhea among children in developing countries and of traveler's diarrhea.
Bo Yang; Zheng Huang; Zhujun He; Yue Yue; Yonghua Zhou; R. Paul Ross; Catherine Stanton; Hao Zhang; Jianxin Zhao; Wei Chen. Protective effect of Bifidobacterium bifidum FSDJN7O5 and Bifidobacterium breve FHNFQ23M3 on diarrhea caused by enterotoxigenic Escherichia coli. Food & Function 2021, 12, 7271 -7282.
AMA StyleBo Yang, Zheng Huang, Zhujun He, Yue Yue, Yonghua Zhou, R. Paul Ross, Catherine Stanton, Hao Zhang, Jianxin Zhao, Wei Chen. Protective effect of Bifidobacterium bifidum FSDJN7O5 and Bifidobacterium breve FHNFQ23M3 on diarrhea caused by enterotoxigenic Escherichia coli. Food & Function. 2021; 12 (16):7271-7282.
Chicago/Turabian StyleBo Yang; Zheng Huang; Zhujun He; Yue Yue; Yonghua Zhou; R. Paul Ross; Catherine Stanton; Hao Zhang; Jianxin Zhao; Wei Chen. 2021. "Protective effect of Bifidobacterium bifidum FSDJN7O5 and Bifidobacterium breve FHNFQ23M3 on diarrhea caused by enterotoxigenic Escherichia coli." Food & Function 12, no. 16: 7271-7282.
Lactobacillus plantarum proliferates inefficiently in milk, mainly because of its lack of cell envelope proteases and its inability to hydrolyse proteins in milk. Our previous study showed that this strain could grow well in milk with the addition of oat and malt extracts. To investigate the usage and preference for polypeptides and oligopeptides for this strain, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), o-phthaldialdehyde (OPA), high-performance liquid chromatography (HPLC), plate counting and other methods were used in this study. The results showed that proteins in fermented milk cannot be absorbed and utilised by L. plantarum, whereas polypeptides and oligopeptides provide available nitrogen sources for their growth. Short-chain peptides were more conducive to absorption and utilisation than long-chain peptides. In particular, peptides with molecular weights in the range of 200–1400 Da in the oat extract and 100–700 Da in the malt extract were preferentially absorbed and utilised.
Yuanzhi Jiang; Yangyang Shi; Rui Li; Feng Hang; Jianxin Zhao; Hao Zhang; Wei Chen. The peptides in oat and malt extracts that are preferentially absorbed by Lactobacillus plantarum and stimulates its proliferation in milk. International Journal of Food Science & Technology 2021, 1 .
AMA StyleYuanzhi Jiang, Yangyang Shi, Rui Li, Feng Hang, Jianxin Zhao, Hao Zhang, Wei Chen. The peptides in oat and malt extracts that are preferentially absorbed by Lactobacillus plantarum and stimulates its proliferation in milk. International Journal of Food Science & Technology. 2021; ():1.
Chicago/Turabian StyleYuanzhi Jiang; Yangyang Shi; Rui Li; Feng Hang; Jianxin Zhao; Hao Zhang; Wei Chen. 2021. "The peptides in oat and malt extracts that are preferentially absorbed by Lactobacillus plantarum and stimulates its proliferation in milk." International Journal of Food Science & Technology , no. : 1.
Branched-chain amino acids (BCAAs) play an important role in lipid metabolism by serving as signal molecules as well as a potential acetyl-CoA source. Our previous study found that in the oleaginous fungus Mucor circinelloides, beta-isopropylmalate dehydrogenase (IPMDH), an important enzyme participating in the key BCAA leucine biosynthesis, was differentially expressed during lipid accumulation phase and has a positive role on lipogenesis. To further analyze its effects on lipogenesis in another oleaginous fungus Mortierella alpina, the IPMDH-encoding gene MaLeuB was homologously expressed. It was found that the total fatty acid content in the recombinant strain was increased by 20.2% compared with the control strain, which correlated with a 4-fold increase in the MaLeuB transcriptional level. Intracellular metabolites analysis revealed significant changes in amino acid biosynthesis and metabolism, tricarboxylic acid cycle and butanoate metabolism; specifically, leucine and isoleucine levels were upregulated by 6.4-fold and 2.2-fold, respectively. Our genetic engineering approach and metabolomics study demonstrated that MaLeuB is involved in fatty acid metabolism in M. alpina by affecting BCAAs metabolism, and this newly discovered role of IPMDH provides a potential bypass route to increase lipogenesis in oleaginous fungi.
Xin Tang; Lulu Chang; Shujie Gu; Hao Zhang; Yong Q. Chen; Haiqin Chen; Jianxin Zhao; Wei Chen. Role of beta-isopropylmalate dehydrogenase in lipid biosynthesis of the oleaginous fungus Mortierella alpina. Fungal Genetics and Biology 2021, 152, 103572 .
AMA StyleXin Tang, Lulu Chang, Shujie Gu, Hao Zhang, Yong Q. Chen, Haiqin Chen, Jianxin Zhao, Wei Chen. Role of beta-isopropylmalate dehydrogenase in lipid biosynthesis of the oleaginous fungus Mortierella alpina. Fungal Genetics and Biology. 2021; 152 ():103572.
Chicago/Turabian StyleXin Tang; Lulu Chang; Shujie Gu; Hao Zhang; Yong Q. Chen; Haiqin Chen; Jianxin Zhao; Wei Chen. 2021. "Role of beta-isopropylmalate dehydrogenase in lipid biosynthesis of the oleaginous fungus Mortierella alpina." Fungal Genetics and Biology 152, no. : 103572.
Psychobiotics are used to treat neurological disorders, including mild cognitive impairment (MCI) and Alzheimer’s disease (AD). However, the mechanisms underlying their neuroprotective effects remain unclear. Herein, we report that the administration of bifidobacteria in an AD mouse model improved behavioral abnormalities and modulated gut dysbiosis. Bifidobacterium breve CCFM1025 and WX treatment significantly improved synaptic plasticity and increased the concentrations of brain-derived neurotrophic factor (BDNF), fibronectin type III domain-containing protein 5 (FNDC5), and postsynaptic density protein 95 (PSD-95). Furthermore, the microbiome and metabolomic profiles of mice indicate that specific bacterial taxa and their metabolites correlate with AD-associated behaviors, suggesting that the gut–brain axis contributes to the pathophysiology of AD. Overall, these findings reveal that B. breve CCFM1025 and WX have beneficial effects on cognition via the modulation of the gut microbiome, and thus represent a novel probiotic dietary intervention for delaying the progression of AD.
Guangsu Zhu; Jianxin Zhao; Hao Zhang; Wei Chen; Gang Wang. Administration of Bifidobacterium breve Improves the Brain Function of Aβ1-42-Treated Mice via the Modulation of the Gut Microbiome. Nutrients 2021, 13, 1602 .
AMA StyleGuangsu Zhu, Jianxin Zhao, Hao Zhang, Wei Chen, Gang Wang. Administration of Bifidobacterium breve Improves the Brain Function of Aβ1-42-Treated Mice via the Modulation of the Gut Microbiome. Nutrients. 2021; 13 (5):1602.
Chicago/Turabian StyleGuangsu Zhu; Jianxin Zhao; Hao Zhang; Wei Chen; Gang Wang. 2021. "Administration of Bifidobacterium breve Improves the Brain Function of Aβ1-42-Treated Mice via the Modulation of the Gut Microbiome." Nutrients 13, no. 5: 1602.
L. fermentum CCFM1139 can decrease P. gingivalis and F. nucleatum colonizations in the oral cavity of rats, and regulate the expression of TNF-α, IL-1β, and IL-8 in the periodontal tissue, and alveolar bone loss caused by periodontitis.
Qiuxiang Zhang; Wanqing Xu; Xianyin Xu; Wenwei Lu; Jianxin Zhao; Hao Zhang; Wei Chen. Effects of Limosilactobacillus fermentum CCFM1139 on experimental periodontitis in rats. Food & Function 2021, 12, 4670 -4678.
AMA StyleQiuxiang Zhang, Wanqing Xu, Xianyin Xu, Wenwei Lu, Jianxin Zhao, Hao Zhang, Wei Chen. Effects of Limosilactobacillus fermentum CCFM1139 on experimental periodontitis in rats. Food & Function. 2021; 12 (10):4670-4678.
Chicago/Turabian StyleQiuxiang Zhang; Wanqing Xu; Xianyin Xu; Wenwei Lu; Jianxin Zhao; Hao Zhang; Wei Chen. 2021. "Effects of Limosilactobacillus fermentum CCFM1139 on experimental periodontitis in rats." Food & Function 12, no. 10: 4670-4678.
In recent years, Blautia has attracted attention for its role in ameliorating host diseases. In particular, Blautia producta DSM 2950 has been considered a potential probiotic due to its ability to mitigate inflammation in poly(I:C) induced HT-29 cells. Thus, to promote the development of indigenous intestinal microorganisms with potential probiotic function, we conducted a comprehensive experimental analysis of DSM 2950 to determine its safety. This comprised a study of its potential virulence genes, antibiotic resistance genes, genomic islands, antibiotic resistance, and hemolytic activity and a 14-day test of its acute oral toxicity in mice. The results indicated no toxin-related virulence genes in the DSM 2950 genome. Most of the genomic islands in DSM 2950 were related to metabolism, rather than virulence expression. DSM 2950 was sensitive to most of the tested antibiotics but was tolerant of treatment with kanamycin, neomycin, clindamycin, or ciprofloxacin, probably because it possessed the corresponding antibiotic resistance genes. Oral acute toxicity tests indicated that the consumption of DSM 2950 does not cause toxic side effects in mice. Overall, the safety profile of DSM 2950 confirmed that it could be a candidate probiotic for use in food and pharmaceutical preparations.
Xuemei Liu; Weiling Guo; Shumao Cui; Xin Tang; Jianxin Zhao; Hao Zhang; Bingyong Mao; Wei Chen. A Comprehensive Assessment of the Safety of Blautia producta DSM 2950. Microorganisms 2021, 9, 908 .
AMA StyleXuemei Liu, Weiling Guo, Shumao Cui, Xin Tang, Jianxin Zhao, Hao Zhang, Bingyong Mao, Wei Chen. A Comprehensive Assessment of the Safety of Blautia producta DSM 2950. Microorganisms. 2021; 9 (5):908.
Chicago/Turabian StyleXuemei Liu; Weiling Guo; Shumao Cui; Xin Tang; Jianxin Zhao; Hao Zhang; Bingyong Mao; Wei Chen. 2021. "A Comprehensive Assessment of the Safety of Blautia producta DSM 2950." Microorganisms 9, no. 5: 908.
Correction for ‘Lactic acid bacteria alleviate polycystic ovarian syndrome by regulating sex hormone related gut microbiota’ by Yufeng He et al., Food Funct., 2020, 11, 5192–5204, DOI: 10.1039/C9FO02554E.
Yufeng He; Qianqian Wang; Xiu Li; Gang Wang; Jianxin Zhao; Hao Zhang; Wei Chen. Correction: Lactic acid bacteria alleviate polycystic ovarian syndrome by regulating sex hormone related gut microbiota. Food & Function 2021, 12, 4720 -4721.
AMA StyleYufeng He, Qianqian Wang, Xiu Li, Gang Wang, Jianxin Zhao, Hao Zhang, Wei Chen. Correction: Lactic acid bacteria alleviate polycystic ovarian syndrome by regulating sex hormone related gut microbiota. Food & Function. 2021; 12 (10):4720-4721.
Chicago/Turabian StyleYufeng He; Qianqian Wang; Xiu Li; Gang Wang; Jianxin Zhao; Hao Zhang; Wei Chen. 2021. "Correction: Lactic acid bacteria alleviate polycystic ovarian syndrome by regulating sex hormone related gut microbiota." Food & Function 12, no. 10: 4720-4721.
Bifidobacteria are among the first colonizers of the human gastrointestinal tract. Different bacterial species use different mechanisms for utilization of various carbon sources in order to establish themselves in the complex microbial ecosystem of the gut. However, these mechanisms still need to be explored. Here, a large gene–phenotype correlation analysis was carried out to explore the metabolic and genetic diversity of bifidobacterial carbohydrate utilization abilities. In this study, we used 21 different carbohydrates to determine the growth phenotypes, the distribution of glycoside hydrolases (GHs), and gene clusters related to the utilization of multiple carbon sources in six human-residential Bifidobacterium species. Five carbohydrates significantly stimulated growth of almost all strains, while the remaining sugars exhibited species- and strain-specificity. Correspondingly, different Bifidobacterium species also had specific GHs involved in fermentation of plant or host glycans. Moreover, we analyzed several carbohydrate utilization gene clusters, such as 2-fucosyllactose (2′FL), sialic acid (SA), and fructooligosaccharide (FOS). In summary, by using 217 bifidobacterial strains and a wide range of growth substrates, our research revealed inter- and intra-species differences in bifidobacterial in terms of carbohydrate utilization. The findings of this study are useful for the process of developing prebiotics for optimum growth of probiotics, especially Bifidobacterium species.
Shijie Liu; Zhifeng Fang; Hongchao Wang; Qixiao Zhai; Feng Hang; Jianxin Zhao; Hao Zhang; Wenwei Lu; Wei Chen. Gene–Phenotype Associations Involving Human-Residential Bifidobacteria (HRB) Reveal Significant Species- and Strain-Specificity in Carbohydrate Catabolism. Microorganisms 2021, 9, 883 .
AMA StyleShijie Liu, Zhifeng Fang, Hongchao Wang, Qixiao Zhai, Feng Hang, Jianxin Zhao, Hao Zhang, Wenwei Lu, Wei Chen. Gene–Phenotype Associations Involving Human-Residential Bifidobacteria (HRB) Reveal Significant Species- and Strain-Specificity in Carbohydrate Catabolism. Microorganisms. 2021; 9 (5):883.
Chicago/Turabian StyleShijie Liu; Zhifeng Fang; Hongchao Wang; Qixiao Zhai; Feng Hang; Jianxin Zhao; Hao Zhang; Wenwei Lu; Wei Chen. 2021. "Gene–Phenotype Associations Involving Human-Residential Bifidobacteria (HRB) Reveal Significant Species- and Strain-Specificity in Carbohydrate Catabolism." Microorganisms 9, no. 5: 883.