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Dairy farm slurry is an important biomass resource that can be used as a fertilizer and in energy utilization and chemical production. This study aimed to establish an innovative ultrasound-assisted electrochemical oxidation (UAEO) digestion method for the rapid and onsite analysis of the heavy metal (HM) contamination level of dairy slurry. The effects of UAEO operating parameters on digestion efficiency were tested based on Cu and Zn concentrations in a dairy slurry sample. The results showed that Cu and Zn digestion efficiency was (96.8 ± 2.6) and (98.5 ± 2.9)%, respectively, with the optimal UAEO operating parameters (digestion time: 45 min; ultrasonic power: 400 W; NaCl concentration: 10 g/L). The digestion recovery rate experiments were then operated with spiked samples to verify the digestion effect on broad-spectrum HMs. When the digestion time reached 45 min, all digestion recovery rates exceeded 90%. Meanwhile, free chlorine concentration, particle size distribution, and micromorphology were investigated to demonstrate the digestion mechanism. It was found that 414 mg/L free chorine had theoretically enough oxidative ability, and the ultrasound intervention could deal with the blocky undissolved particles attributed to its crushing capacity. The results of particle size distribution showed that the total volume and bulky particle proportion had an obvious decline. The micromorphology demonstrated that the ultrasound intervention fragmented the bulky particles, and electrochemical oxidation made irregular blocky structures form arc edge and cellular structures. The aforementioned results indicated that UAEO was a novel and efficient method. It was fast and convenient. Additionally, it ensured digestion efficiency and thus had a good application prospect.
Chenyu Li; Bin Xue; Shang Wang; Xi Zhang; Chen Zhao; Xiaobo Yang; Run Zhao; Lin Dai; Shengqi Su; Haoqi Xu; Zhiqiang Shen; Zhigang Qiu; Jingfeng Wang. An Innovative Digestion Method: Ultrasound-Assisted Electrochemical Oxidation for the Onsite Extraction of Heavy Metal Elements in Dairy Farm Slurry. Materials 2021, 14, 4562 .
AMA StyleChenyu Li, Bin Xue, Shang Wang, Xi Zhang, Chen Zhao, Xiaobo Yang, Run Zhao, Lin Dai, Shengqi Su, Haoqi Xu, Zhiqiang Shen, Zhigang Qiu, Jingfeng Wang. An Innovative Digestion Method: Ultrasound-Assisted Electrochemical Oxidation for the Onsite Extraction of Heavy Metal Elements in Dairy Farm Slurry. Materials. 2021; 14 (16):4562.
Chicago/Turabian StyleChenyu Li; Bin Xue; Shang Wang; Xi Zhang; Chen Zhao; Xiaobo Yang; Run Zhao; Lin Dai; Shengqi Su; Haoqi Xu; Zhiqiang Shen; Zhigang Qiu; Jingfeng Wang. 2021. "An Innovative Digestion Method: Ultrasound-Assisted Electrochemical Oxidation for the Onsite Extraction of Heavy Metal Elements in Dairy Farm Slurry." Materials 14, no. 16: 4562.
Infection caused by pathogenic bacteria carrying antibiotic resistance genes (ARGs) is a serious challenge to human health. Water environment, including water and surface sediments, is an important repository of ARGs, and the activity of aquatic animal can affect the development of ARG pollution in the water environment. Macrobenthic invertebrates are an important component of aquatic ecosystems, and their effects on ARG development in aquatic environments remain unreported. The distribution of ARGs, including tetA gene, sul2 gene, and kan gene, in Chironomidae larvae is demonstrated in this study for the first time. The ARG distribution was related to sampling points, metal elements, and seasons. Animal models demonstrated that Chironomidae larvae enriched ARGs from water and passed them on to downstream predators in the food chain. Conjugative transfer mediated by resistant plasmids was crucial in the spread of ARG in Chironomidae larvae, and upregulated expression of trfAp gene and trbBp gene was the molecular mechanism. Escherichia in Proteobacteria and Flavobacterium in Bacteroidetes, which are gram-negative bacteria in Chironomidae larvae, are the primary host bacteria of ARGs confirmed via resistance screening and DNA sequencing of V4 region of 16S rRNA gene. Feeding experiments further confirmed that ARGs from Chironomidae larvae can be enriched in the fish gut. Research gaps in food chain between sediments and fish are addressed in this study, and Chironomidae larvae is an important enricher of ARGs in the freshwater environment.
Chengshi Ding; Jing Ma; Wanxiang Jiang; Hanyu Zhao; Mengmeng Shi; Guoqing Cui; Tongdi Yan; Qi Wang; Junwen Li; Zhigang Qiu. Chironomidae larvae: A neglected enricher of antibiotic resistance genes in the food chain of freshwater environments. Environmental Pollution 2021, 285, 117486 .
AMA StyleChengshi Ding, Jing Ma, Wanxiang Jiang, Hanyu Zhao, Mengmeng Shi, Guoqing Cui, Tongdi Yan, Qi Wang, Junwen Li, Zhigang Qiu. Chironomidae larvae: A neglected enricher of antibiotic resistance genes in the food chain of freshwater environments. Environmental Pollution. 2021; 285 ():117486.
Chicago/Turabian StyleChengshi Ding; Jing Ma; Wanxiang Jiang; Hanyu Zhao; Mengmeng Shi; Guoqing Cui; Tongdi Yan; Qi Wang; Junwen Li; Zhigang Qiu. 2021. "Chironomidae larvae: A neglected enricher of antibiotic resistance genes in the food chain of freshwater environments." Environmental Pollution 285, no. : 117486.
The prevalence of various antibiotic resistance genes (ARGs) and resistant bacteria has caused global public health risks. The carrier transport mediated by phages or membrane vesicles is an important way for horizontal transfer of ARGs. Nano metal oxide particles (NMOPs), which can enter cell through the cell membrane, may be used as the carriers of genes. However, whether they can be used as transmembrane delivery vectors for the horizontal ARG transfer remains unknown. Here, we set up a model of MONPs-mediated transfer of ARGs, and demonstrate that NMOPs, especially for nano-Al2O3, can act as carriers mediating the transduction-like ARG transformation in water. The highest transfer rate mediated by nano-Al2O3 is 4.53 × 104 cfu/mmol, and it is 104 times higher than that of control. Nano-Al2O3 can combine with plasmid coding for ARGs to form high-density package and prevent ARGs from degradation by endonuclease. The results of superresolution fluorescence microscopy and transmission electron microscopy show that nano-Al2O3 can carry ARGs for transmembrane transport. Genome-wide transcription microarray and qPCR indicate that SOS response was closely related to transduction-like ARG transformation mediated by nano-Al2O3. This study is the first to demonstrate that as a new transmembrane carrier, nano-Al2O3 can also cause ARGs diffusion in water.
Chengshi Ding; Min Jin; Jing Ma; Zhaoli Chen; Zhiqiang Shen; Dong Yang; Danyang Shi; Weili Liu; Meiling Kang; Jingfeng Wang; Junwen Li; Zhigang Qiu. Nano-Al2O3 can mediate transduction-like transformation of antibiotic resistance genes in water. Journal of Hazardous Materials 2020, 405, 124224 .
AMA StyleChengshi Ding, Min Jin, Jing Ma, Zhaoli Chen, Zhiqiang Shen, Dong Yang, Danyang Shi, Weili Liu, Meiling Kang, Jingfeng Wang, Junwen Li, Zhigang Qiu. Nano-Al2O3 can mediate transduction-like transformation of antibiotic resistance genes in water. Journal of Hazardous Materials. 2020; 405 ():124224.
Chicago/Turabian StyleChengshi Ding; Min Jin; Jing Ma; Zhaoli Chen; Zhiqiang Shen; Dong Yang; Danyang Shi; Weili Liu; Meiling Kang; Jingfeng Wang; Junwen Li; Zhigang Qiu. 2020. "Nano-Al2O3 can mediate transduction-like transformation of antibiotic resistance genes in water." Journal of Hazardous Materials 405, no. : 124224.
The rapid spread of antibiotic resistance genes (ARGs) is a great challenge to the ecological safety and human health. The intestine of humans and animals is an important site for the increase and spread of ARGs due to the great diversity and abundance of microorganisms in the intestinal microecology. ARGs, including the intracellular (iARGs) and the extracellular (eARGs) ARGs, are usually introduced into the intestinal tract through the diet, and the iARGs are colonized and spread in the intestinal microbiota with the help of the host bacteria. However, whether the eARGs can enter the intestinal microorganisms in the absence of host bacteria is not known. Here, we show the transformation and the diffusion of the ampramycin resistance gene (Ap) carried by the free plasmid RK2 in the intestinal microbiota of mice. After two days of consecutive gavage with free RK2, the intracellular Ap gene increases from days 0–8 in the feces of mice, and has remained constant. Bacterial transformation happens in the small intestine, including proximal and distal jejuna and proximal and distal ilea, at the early stage (first two days), and the intracellular RK2 is diffused into the intestinal microbiota of mice by conjugation on days 2–8 day, which is based on the distribution of eARG and iARG and the mRNA expression levels of trbBp, trfAp, korA, korB, and trbA. The characteristics of ARGs susceptible microbiota for transformation are analyzed using 16s rRNA gene sequencing, transmission electron microscopy, and flow cytometric. The ingestion of RK2 affects the composition of intestinal microbiota especially for Proteobacteria, and the antibiotic residue promotes the increase in Escherichia coli. These findings are important to assess the risk of ARGs, especially the eARGs in the intestinal microecology.
Chengshi Ding; Dong Yang; Jing Ma; Min Jin; Zhiqiang Shen; Danyang Shi; Zhongjing Tian; Meiling Kang; Junwen Li; Zhigang Qiu. Effects of free antibiotic resistance genes in the environment on intestinal microecology of mice. Ecotoxicology and Environmental Safety 2020, 204, 111119 .
AMA StyleChengshi Ding, Dong Yang, Jing Ma, Min Jin, Zhiqiang Shen, Danyang Shi, Zhongjing Tian, Meiling Kang, Junwen Li, Zhigang Qiu. Effects of free antibiotic resistance genes in the environment on intestinal microecology of mice. Ecotoxicology and Environmental Safety. 2020; 204 ():111119.
Chicago/Turabian StyleChengshi Ding; Dong Yang; Jing Ma; Min Jin; Zhiqiang Shen; Danyang Shi; Zhongjing Tian; Meiling Kang; Junwen Li; Zhigang Qiu. 2020. "Effects of free antibiotic resistance genes in the environment on intestinal microecology of mice." Ecotoxicology and Environmental Safety 204, no. : 111119.
Currently, due to abuse in the use of human antibiotics and the weak regulatory control that the authorities have over sewage discharge and manure management, antibiotic resistance genes (ARGs) have become a new type of environmental pollutant. Three different natural water bodies (Poyang Lake, Haihe River and Qingdao No.1 Bathing Beach seawater) were sampled during the same periods to conduct a longitudinal comparison of distribution. The distribution and expression of 11 ARGs in 20 species were studied, and the correlations between the expression and the distribution of time and space of the ARGs in different water bodies were also analyzed. With the exception of ermA, blaNDM-1 and vanA, which were not detected in seawater, the other ARGs could be detected in all three water bodies. Tetracycline resistance genes (tetC, tetM and tetQ) in the seawater and Haihe River had even reached 100%, and sulfa ARGs (sul1 and sul2) in the seawater and Poyang Lake, as well as sul2 and sul3 in the Haihe River, had also reached 100%. The ARG pollution in Haihe River was much more serious, since 14 and 17 of 20 ARG species were significantly higher compared with seawater and Poyang Lake, respectively. Some ARGs also had a high absolute abundance. The absolute abundance of macrolide resistance genes (ermB) in seawater was as high as 8.61 × 107 copies/L, and the anti-tuberculosis resistant genes (rpoB and katG) in the Haihe River Basin were highly abundant at 1.32 × 106 copies/L and 1.06 × 107 copies/L, respectively. This indicates that ARGs have gradually become more diverse and extensive in natural water bodies. The results of a redundancy analysis (RDA) of the three water bodies showed that although each water body is affected by different factors in space and time, overall, the presence of AGRs is closely related to the production and life of human beings and the migration of animals.
Sicong Su; Chenyu Li; Jiping Yang; Qunying Xu; Zhigang Qiu; Bin Xue; Shang Wang; Chen Zhao; Zhonghai Xiao; Jingfeng Wang; Zhiqiang Shen. Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea. International Journal of Environmental Research and Public Health 2020, 17, 552 .
AMA StyleSicong Su, Chenyu Li, Jiping Yang, Qunying Xu, Zhigang Qiu, Bin Xue, Shang Wang, Chen Zhao, Zhonghai Xiao, Jingfeng Wang, Zhiqiang Shen. Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea. International Journal of Environmental Research and Public Health. 2020; 17 (2):552.
Chicago/Turabian StyleSicong Su; Chenyu Li; Jiping Yang; Qunying Xu; Zhigang Qiu; Bin Xue; Shang Wang; Chen Zhao; Zhonghai Xiao; Jingfeng Wang; Zhiqiang Shen. 2020. "Distribution of Antibiotic Resistance Genes in Three Different Natural Water Bodies-A Lake, River and Sea." International Journal of Environmental Research and Public Health 17, no. 2: 552.
Enteric viruses in surface water pose considerable risk to morbidity in populations living around water catchments and promote outbreaks of waterborne diseases. However, due to poor understanding of the correlation between water quality and the presence of human enteric viruses, the failure to assess viral contamination through alternative viral indicators makes it difficult to control disease transmission. We investigated the occurrence of Enteroviruses (EnVs), Rotaviruses (HRVs), Astroviruses (AstVs), Noroviruses GII (HuNoVs GII) and Adenoviruses (HAdVs) from Jinhe River over 4 years and analyzed their correlation with physicochemical and bacterial parameters in water samples. The findings showed that all target viruses were detected in water at frequencies of 91.7% for HAdVs, 81.3% for HuNoVs GII, 79.2% for EnVs and AstVs, and 70.8% for HRVs. These viruses had a seasonal pattern, which showed that EnVs were abundant in summer but rare in winter, while HAdVs, HRVs, AstVs, and HuNoVs GII exhibited opposite seasonal trends. Pearson correlation analysis showed that total coliforms (TC) was significantly positively correlated with EnVs concentrations while no consistent significant correlations were observed between bacterial indices and viruses that precipitate acute gastroenteritis. Taken together, the findings provide insights into alternative viral indicators, suggesting that TC is a potentially promising candidate for assessment of EnVs contamination. However, it failed to predict the presence of HAdVs, HRVs, AstVs, and HuNoVs GΙΙ in surface water across the city of Tianjin.
Jing Miao; Xuan Guo; Weili Liu; Dong Yang; Zhiqiang Shen; Zhigang Qiu; Xiang Chen; Kunming Zhang; Hui Hu; Jing Yin; Zhongwei Yang; Junwen Li; Min Jin. Total coliforms as an indicator of human enterovirus presence in surface water across Tianjin city, China. BMC Infectious Diseases 2018, 18, 542 .
AMA StyleJing Miao, Xuan Guo, Weili Liu, Dong Yang, Zhiqiang Shen, Zhigang Qiu, Xiang Chen, Kunming Zhang, Hui Hu, Jing Yin, Zhongwei Yang, Junwen Li, Min Jin. Total coliforms as an indicator of human enterovirus presence in surface water across Tianjin city, China. BMC Infectious Diseases. 2018; 18 (1):542.
Chicago/Turabian StyleJing Miao; Xuan Guo; Weili Liu; Dong Yang; Zhiqiang Shen; Zhigang Qiu; Xiang Chen; Kunming Zhang; Hui Hu; Jing Yin; Zhongwei Yang; Junwen Li; Min Jin. 2018. "Total coliforms as an indicator of human enterovirus presence in surface water across Tianjin city, China." BMC Infectious Diseases 18, no. 1: 542.
While nanoparticles exert bactericidal effects through the generation of reactive oxygen species (ROS), the processes of the internalization of and the direct physical damage caused by iron oxide nanoparticles are not completely clear. We hypothesize that direct physical or mechanical damage of the cell membrane and cytoplasmic integrity by nanoparticles is another major cause of bacterial death besides ROS. The aim of this study is to investigate the process of the internalization of iron oxide nanoparticles, and to evaluate the effect of direct physical or mechanical damage on bacterial cell growth and death. The results demonstrate that iron oxide nanoparticles not only inhibited E. coli cell growth, but also caused bacterial cell death. Iron oxide nanoparticles produced significantly elevated ROS levels in bacteria. Transmission electronic microscopy demonstrated that iron oxide nanoparticles were internalized into and condensed the cytoplasm. Strikingly, we observed that the internalized nanoparticles caused intracellular vacuole formation, instead of simply adsorbing thereon; and formed clusters on the bacterial surface and tore up the outer cell membrane to release cytoplasm. This is the first time that the exact process of the internalization of iron oxide nanoparticles has been observed. We speculate that the intracellular vacuole formation and direct physical or mechanical damage caused by the iron oxide nanoparticles caused the bactericidal effect, along with the effects of ROS.
Yunqiao Li; Dong Yang; Shang Wang; Chenyu Li; Bin Xue; Lin Yang; Zhiqiang Shen; Min Jin; Jingfeng Wang; Zhigang Qiu. The Detailed Bactericidal Process of Ferric Oxide Nanoparticles on E. coli. Molecules 2018, 23, 606 .
AMA StyleYunqiao Li, Dong Yang, Shang Wang, Chenyu Li, Bin Xue, Lin Yang, Zhiqiang Shen, Min Jin, Jingfeng Wang, Zhigang Qiu. The Detailed Bactericidal Process of Ferric Oxide Nanoparticles on E. coli. Molecules. 2018; 23 (3):606.
Chicago/Turabian StyleYunqiao Li; Dong Yang; Shang Wang; Chenyu Li; Bin Xue; Lin Yang; Zhiqiang Shen; Min Jin; Jingfeng Wang; Zhigang Qiu. 2018. "The Detailed Bactericidal Process of Ferric Oxide Nanoparticles on E. coli." Molecules 23, no. 3: 606.
The aqueous environment is one of many reservoirs of antibiotic resistance genes (ARGs). Fish, as important aquatic animals which possess ideal intestinal niches for bacteria to grow and multiply, may ingest antibiotic resistance bacteria from aqueous environment. The fish gut would be a suitable environment for conjugal gene transfer including those encoding antibiotic resistance. However, little is known in relation to the impact of ingested ARGs or antibiotic resistance bacteria (ARB) on gut microbiota. Here, we applied the cultivation method, qPCR, nuclear molecular genetic marker and 16S rDNA amplicon sequencing technologies to develop a plasmid-mediated ARG transfer model of zebrafish. Furthermore, we aimed to investigate the dissemination of ARGs in microbial communities of zebrafish guts after donors carrying self-transferring plasmids that encode ARGs were introduced in aquaria. On average, 15% of faecal bacteria obtained ARGs through RP4-mediated conjugal transfer. The hindgut was the most important intestinal region supporting ARG dissemination, with concentrations of donor and transconjugant cells almost 25 times higher than those of other intestinal segments. Furthermore, in the hindgut where conjugal transfer occurred most actively, there was remarkable upregulation of the mRNA expression of the RP4 plasmid regulatory genes, trbBp and trfAp. Exogenous bacteria seem to alter bacterial communities by increasing Escherichia and Bacteroides species, while decreasing Aeromonas compared with control groups. We identified the composition of transconjugants and abundance of both cultivable and uncultivable bacteria (the latter accounted for 90.4%–97.2% of total transconjugants). Our study suggests that aquatic animal guts contribute to the spread of ARGs in water environments.
Jialun Fu; Dong Yang; Wang Jingfeng; Weili Liu; Xin Zhao; Chenyu Li; Tianyu Zhao; Jingfeng Wang; Zhixian Gao; Zhiqiang Shen; Zhigang Qiu; Jun-Wen Li. Aquatic animals promote antibiotic resistance gene dissemination in water via conjugation: Role of different regions within the zebra fish intestinal tract, and impact on fish intestinal microbiota. Molecular Ecology 2017, 26, 5318 -5333.
AMA StyleJialun Fu, Dong Yang, Wang Jingfeng, Weili Liu, Xin Zhao, Chenyu Li, Tianyu Zhao, Jingfeng Wang, Zhixian Gao, Zhiqiang Shen, Zhigang Qiu, Jun-Wen Li. Aquatic animals promote antibiotic resistance gene dissemination in water via conjugation: Role of different regions within the zebra fish intestinal tract, and impact on fish intestinal microbiota. Molecular Ecology. 2017; 26 (19):5318-5333.
Chicago/Turabian StyleJialun Fu; Dong Yang; Wang Jingfeng; Weili Liu; Xin Zhao; Chenyu Li; Tianyu Zhao; Jingfeng Wang; Zhixian Gao; Zhiqiang Shen; Zhigang Qiu; Jun-Wen Li. 2017. "Aquatic animals promote antibiotic resistance gene dissemination in water via conjugation: Role of different regions within the zebra fish intestinal tract, and impact on fish intestinal microbiota." Molecular Ecology 26, no. 19: 5318-5333.
Bacteriophages are widely used to the treatment of drug-resistant bacteria and the improvement of food safety through bacterial lysis. However, the limited investigations on bacteriophage restrict their further application. In this study, a novel and highly efficient method was developed for isolating bacteriophage from water based on the electropositive silica gel particles (ESPs) method. To optimize the ESPs method, we evaluated the eluent type, flow rate, pH, temperature, and inoculation concentration of bacteriophage using bacteriophage f2. The quantitative detection reported that the recovery of the ESPs method reached over 90%. The qualitative detection demonstrated that the ESPs method effectively isolated 70% of extremely low-concentration bacteriophage (100 PFU/100L). Based on the host bacteria composed of 33 standard strains and 10 isolated strains, the bacteriophages in 18 water samples collected from the three sites in the Tianjin Haihe River Basin were isolated by the ESPs and traditional methods. Results showed that the ESPs method was significantly superior to the traditional method. The ESPs method isolated 32 strains of bacteriophage, whereas the traditional method isolated 15 strains. The sample isolation efficiency and bacteriophage isolation efficiency of the ESPs method were 3.28 and 2.13 times higher than those of the traditional method. The developed ESPs method was characterized by high isolation efficiency, efficient handling of large water sample size and low requirement on water quality.
Weili Liu; Chao Li; Zhi-Gang Qiu; Min Jin; Jing-Feng Wang; Ng Yang; Zhong-Hai Xiao; Zhao-Kang Yuan; Jun-Wen Li; Qun-Ying Xu; Zhi-Qiang Shen. Development of a novel and highly efficient method of isolating bacteriophages from water. Journal of Microbiological Methods 2017, 139, 143 -149.
AMA StyleWeili Liu, Chao Li, Zhi-Gang Qiu, Min Jin, Jing-Feng Wang, Ng Yang, Zhong-Hai Xiao, Zhao-Kang Yuan, Jun-Wen Li, Qun-Ying Xu, Zhi-Qiang Shen. Development of a novel and highly efficient method of isolating bacteriophages from water. Journal of Microbiological Methods. 2017; 139 ():143-149.
Chicago/Turabian StyleWeili Liu; Chao Li; Zhi-Gang Qiu; Min Jin; Jing-Feng Wang; Ng Yang; Zhong-Hai Xiao; Zhao-Kang Yuan; Jun-Wen Li; Qun-Ying Xu; Zhi-Qiang Shen. 2017. "Development of a novel and highly efficient method of isolating bacteriophages from water." Journal of Microbiological Methods 139, no. : 143-149.
Human rotaviruses (HRVs) are the major cause of acute diarrhea in infants and young children. Here, a real-time reverse transcription polymerase chain reaction assay targeting the rotaviral VP4 gene (VP4-RT-qPCR) was established to evaluate the inactivation of HRV upon chlorine disinfection, based on a previous report that damage to the 1227-2354bp region of the VP4 gene was associated with eliminated HRV infectivity by chlorine. In this study, inactivation of HRV by 0.6mg/L free chlorine was assessed in phosphate buffered saline (PBS; pH 7.2), and tap and river water samples, using both TCID50 and RT-qPCR (VP2- and VP4-RT-qPCR) assays, respectively. Among the samples tested, the VP2-RT-qPCR method did not show significant inactivation after chlorine disinfection; however, the reduction in VP4-RT-qPCR signal was correlated with decreased HRV infectivity. Moreover, the higher sensitivity of the VP4-RT-qPCR assay allowed for assessment of chlorine HRV inactivation at longer exposure times compared with the conventional TCID50 assay. Collectively, these results indicated that the VP4-RT-qPCR assay is a rapid, sensitive, and reliable tool to detect infectious HRV following chlorine inactivation, and highlights the potential for further development of qPCR/RT-qPCR assays to provide information regarding viral infectivity from drinking water plants.
Bin Xue; Chenyu Li; Bin Zhang; Tianyu Zhao; Zhiqiang Shen; Zhigang Qiu; Min Jin; Jingfeng Wang; Junwen Li. Quantitative reverse transcription PCR to determine the inactivation of Human Rotavirus by chlorine. International Journal of Hygiene and Environmental Health 2017, 220, 719 -725.
AMA StyleBin Xue, Chenyu Li, Bin Zhang, Tianyu Zhao, Zhiqiang Shen, Zhigang Qiu, Min Jin, Jingfeng Wang, Junwen Li. Quantitative reverse transcription PCR to determine the inactivation of Human Rotavirus by chlorine. International Journal of Hygiene and Environmental Health. 2017; 220 (4):719-725.
Chicago/Turabian StyleBin Xue; Chenyu Li; Bin Zhang; Tianyu Zhao; Zhiqiang Shen; Zhigang Qiu; Min Jin; Jingfeng Wang; Junwen Li. 2017. "Quantitative reverse transcription PCR to determine the inactivation of Human Rotavirus by chlorine." International Journal of Hygiene and Environmental Health 220, no. 4: 719-725.
Antibiotic failure is occurring worldwide. In a routine surveillance study on antibiotic resistance genes (ARGs) in natural water bodies, we noted the detection of colistin-resistance gene mcr-1, previously identified in Escherichia coli and Klebsiella pneumoniae isolates from human beings and animals in several countries. The mcr-1 gene might be present in water environments, because aquatic ecosystems are recognized as reservoirs for antibiotic resistant bacteria (ARB) and ARGs. In this study, a qPCR assay was developed to monitor and quantify the mcr-1 gene in the Haihe River, China. The results showed that all 18 samples collected from different locations over 6 months along the Haihe River were positive for the mcr-1 gene, and the highest level of mcr-1 reached 3.81 × 105 gene copies (GC) per liter of water. This is the first study to quantify mcr-1 in a natural water system by qPCR. Our findings highlight the potential for this antibiotic resistance determinant to spread extensively, suggesting a significant health and ecological impact.
Dong Yang; Zhigang Qiu; Zhiqiang Shen; Hong Zhao; Min Jin; Huaying Li; Weili Liu; Jun-Wen Li. The Occurrence of the Colistin Resistance Gene mcr-1 in the Haihe River (China). International Journal of Environmental Research and Public Health 2017, 14, 576 .
AMA StyleDong Yang, Zhigang Qiu, Zhiqiang Shen, Hong Zhao, Min Jin, Huaying Li, Weili Liu, Jun-Wen Li. The Occurrence of the Colistin Resistance Gene mcr-1 in the Haihe River (China). International Journal of Environmental Research and Public Health. 2017; 14 (6):576.
Chicago/Turabian StyleDong Yang; Zhigang Qiu; Zhiqiang Shen; Hong Zhao; Min Jin; Huaying Li; Weili Liu; Jun-Wen Li. 2017. "The Occurrence of the Colistin Resistance Gene mcr-1 in the Haihe River (China)." International Journal of Environmental Research and Public Health 14, no. 6: 576.
The development of pathogenic mechanisms, specific antiviral treatments and preventive vaccines for hepatitis C virus (HCV) infection has been limited due to lack of cell culture models that can naturally imitate the entire HCV life cycle. Here, we established an HCV cell culture model based on human fetal liver stem cells (hFLSCs) that supports the entire blood‐borne hepatitis C virus (bbHCV) life cycle. More than 90% of cells remained infected by various genotypes. bbHCV was efficiently propagated, and progeny virus were infectious to hFLSCs. The virus could be passed efficiently between cells. The viral infectivity was partially blocked by specific antibodies or small interfering RNA against HCV entry factors, whereas HCV replication was inhibited by antiviral drugs. We observed viral particles of approximately 55 nm in diameter in both cell culture media and infected cells after bbHCV infection. Conclusion: Our data show that the entire bbHCV life cycle could be naturally imitated in hFLSCs. This model is expected to provide a powerful tool for exploring the process and the mechanism of bbHCV infection at the cellular level and for evaluating the treatment and preventive strategies of bbHCV infection. (Hepatology 2017;66:1045‐1057).
Xuan Guo; Shu Wang; Zhi-Gang Qiu; Ya-Ling Dou; Wei-Li Liu; Ng Yang; Zhi-Qiang Shen; Zhao-Li Chen; Jing-Feng Wang; Bin Zhang; Xin-Wei Wang; Xiang-Fei Guo; Xue-Lian Zhang; Min Jin; Jun-Wen Li. Efficient replication of blood-borne hepatitis C virus in human fetal liver stem cells. Hepatology 2017, 66, 1045 -1057.
AMA StyleXuan Guo, Shu Wang, Zhi-Gang Qiu, Ya-Ling Dou, Wei-Li Liu, Ng Yang, Zhi-Qiang Shen, Zhao-Li Chen, Jing-Feng Wang, Bin Zhang, Xin-Wei Wang, Xiang-Fei Guo, Xue-Lian Zhang, Min Jin, Jun-Wen Li. Efficient replication of blood-borne hepatitis C virus in human fetal liver stem cells. Hepatology. 2017; 66 (4):1045-1057.
Chicago/Turabian StyleXuan Guo; Shu Wang; Zhi-Gang Qiu; Ya-Ling Dou; Wei-Li Liu; Ng Yang; Zhi-Qiang Shen; Zhao-Li Chen; Jing-Feng Wang; Bin Zhang; Xin-Wei Wang; Xiang-Fei Guo; Xue-Lian Zhang; Min Jin; Jun-Wen Li. 2017. "Efficient replication of blood-borne hepatitis C virus in human fetal liver stem cells." Hepatology 66, no. 4: 1045-1057.
Antibiotic resistance genes (ARGs) have emerged as key factors in wastewater environmental contaminants and continue to pose a challenge for wastewater treatment processes. With the aim of investigating the performance of granular sludge system when treating wastewater containing a considerable amount of ARGs, a lab-scale granular sequencing batch reactor (GSBR) where flocculent and granular sludge coexisted was designed. The results showed that after inoculation of donor strain NH4(+)-N purification efficiency diminished from 94.7% to 32.8% and recovered to 95.2% after 10 days. Meanwhile, RP4 plasmid had varying effects on different forms of microbial aggregates. As the size of aggregates increased, the abundance of RP4 in sludge decreased. The residence time of RP4 in granules with particle size exceeding 0.9 mm (14 days) was far shorter than that in flocculent sludge (26 days). Therefore, our studies conclude that with increasing number of ARGs being detected in wastewater, the use of granular sludge system in wastewater treatment processes will allow the reduction of ARGs transmissions and lessen potential ecological threats.
Wenci Zou; Bin Xue; Weijia Zhi; Tianyu Zhao; Dong Yang; Zhigang Qiu; Zhiqiang Shen; Junwen Li; Bin Zhang; Jingfeng Wang. Effects of antibiotic resistance genes on the performance and stability of different microbial aggregates in a granular sequencing batch reactor. Journal of Hazardous Materials 2016, 304, 343 -351.
AMA StyleWenci Zou, Bin Xue, Weijia Zhi, Tianyu Zhao, Dong Yang, Zhigang Qiu, Zhiqiang Shen, Junwen Li, Bin Zhang, Jingfeng Wang. Effects of antibiotic resistance genes on the performance and stability of different microbial aggregates in a granular sequencing batch reactor. Journal of Hazardous Materials. 2016; 304 ():343-351.
Chicago/Turabian StyleWenci Zou; Bin Xue; Weijia Zhi; Tianyu Zhao; Dong Yang; Zhigang Qiu; Zhiqiang Shen; Junwen Li; Bin Zhang; Jingfeng Wang. 2016. "Effects of antibiotic resistance genes on the performance and stability of different microbial aggregates in a granular sequencing batch reactor." Journal of Hazardous Materials 304, no. : 343-351.
Yuan Gao; Hong-Jing Nie; Ng Yang; Cheng-Shi Ding; Min Jin; Zhi-Qiang Chen; Zhi-Gang Qiu; Xuan Guo; Zhao-Lila Chen; Jun-Wen Li. [Changes of the mitochondrial DNA copy number and the antioxidant system in the PBMC of hepatocellular carcinoma]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 2016, 32, 1 .
AMA StyleYuan Gao, Hong-Jing Nie, Ng Yang, Cheng-Shi Ding, Min Jin, Zhi-Qiang Chen, Zhi-Gang Qiu, Xuan Guo, Zhao-Lila Chen, Jun-Wen Li. [Changes of the mitochondrial DNA copy number and the antioxidant system in the PBMC of hepatocellular carcinoma]. Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology. 2016; 32 (1):1.
Chicago/Turabian StyleYuan Gao; Hong-Jing Nie; Ng Yang; Cheng-Shi Ding; Min Jin; Zhi-Qiang Chen; Zhi-Gang Qiu; Xuan Guo; Zhao-Lila Chen; Jun-Wen Li. 2016. "[Changes of the mitochondrial DNA copy number and the antioxidant system in the PBMC of hepatocellular carcinoma]." Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology 32, no. 1: 1.
The potential risks of nano-materials and the spread of antibiotic resistance genes (ARGs) have become two major global public concerns. Studies have confirmed that nano-alumina can promote the spread of ARGs mediated by plasmids. Nano-titanium dioxide (TiO2), an excellent photocatalytic nano-material, has been widely used and is often present in aqueous environments. At various nano-material concentrations, bacterial density, matting time, and matting temperature, nano-TiO2 can significantly promote the conjugation of RP4 plasmid in Escherichia coli. We developed a mathematical model to quantitatively describe the conjugation process and used this model to evaluate the effects of nano-TiO2 on the spread of ARGs. We obtained analytical solutions for total and resistant bacteria, which were enumerated by the abundance of genetic loci unique to the plasmid and the chromosome using qPCR. Our results showed that the mathematic model was able to fit the experimental data well and can be used to quantitatively evaluate the effects of nano-TiO2. According to our model, the presence of nano-TiO2 decreased the bacterial growth rate from 0.0360 to 0.0323 min(-1) and increased the conjugative transfer rate from 6.69 × 10(-12) to 3.93 × 10(-10 )mL cell(-1) min(-1). These results indicate that nano-TiO2 inhibited bacterial growth and promoted conjugation simultaneously. The data for morphology and mRNA expression also demonstrated this phenomenon. Our results confirm that environmental nano-TiO2 may cause the spread of ARGs and thus poses an environmental risk. In addition, we provide a potential method for monitoring changes in ARGs that result from conjugation and evaluating the effects of antimicrobial substances on ARG expression.
Zhigang Qiu; Zhiqiang Shen; Shen Zhiqiang; Wang Jingfeng; Dong Yang; Jingfeng Wang; Bin Zhang; Zhongwei Yang; Ding Chengshi; Xinwei Wang; Chengshi Ding; Daning Wang; Chen Zhaoli. Effects of nano-TiO2on antibiotic resistance transfer mediated by RP4 plasmid. Nanotoxicology 2015, 9, 895 -904.
AMA StyleZhigang Qiu, Zhiqiang Shen, Shen Zhiqiang, Wang Jingfeng, Dong Yang, Jingfeng Wang, Bin Zhang, Zhongwei Yang, Ding Chengshi, Xinwei Wang, Chengshi Ding, Daning Wang, Chen Zhaoli. Effects of nano-TiO2on antibiotic resistance transfer mediated by RP4 plasmid. Nanotoxicology. 2015; 9 (7):895-904.
Chicago/Turabian StyleZhigang Qiu; Zhiqiang Shen; Shen Zhiqiang; Wang Jingfeng; Dong Yang; Jingfeng Wang; Bin Zhang; Zhongwei Yang; Ding Chengshi; Xinwei Wang; Chengshi Ding; Daning Wang; Chen Zhaoli. 2015. "Effects of nano-TiO2on antibiotic resistance transfer mediated by RP4 plasmid." Nanotoxicology 9, no. 7: 895-904.
This paper presents a functional nanoparticle-enhanced enzyme-linked immunosorbent assay (FNP-ELISA) for detection of enterohemorrhagic Escherichia coli (EHEC) O157:H7. Immunomagnetic nanoparticles (IMMPs) conjugated with monoclonal anti-O157:H7 antibody were used to capture E. coli O157:H7. Beacon gold nanoparticles (B-GNPs) coated with polyclonal anti-O157:H7 and biotin single-stranded DNA (B-DNA) were then subjective to immunoreaction with E. coli O157:H7, which was followed by streptavidin-horseradish peroxidase (Strep-HRP) conjugated with B-GNPs based on a biotin-avidin system. The solutions containing E. coli O157:H7, IMMPs, B-GNPs, and Strep-HRP were collected for detecting color change. The signal was significantly amplified with detection limits of 68 CFU mL-1 in PBS and 6.8 × 102 to 6.8 × 103 CFU mL-1 in the food samples. The FNP-ELISA method developed in this study was two orders of magnitude more sensitive than immunomagnetic separation ELISA (IMS-ELISA) and four orders of magnitude more sensitive than C-ELISA. The entire detection process of E. coli O157:H7 lasted only 3 h, and thus FNP-ELISA is considered as a time-saving method.
Zhiqiang Shen; Nannan Hou; Min Jin; Zhigang Qiu; Jingfeng Wang; Bin Zhang; Xinwei Wang; Jie Wang; Dongsheng Zhou; Junwen Li. A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles. Gut Pathogens 2014, 6, 14 -14.
AMA StyleZhiqiang Shen, Nannan Hou, Min Jin, Zhigang Qiu, Jingfeng Wang, Bin Zhang, Xinwei Wang, Jie Wang, Dongsheng Zhou, Junwen Li. A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles. Gut Pathogens. 2014; 6 (1):14-14.
Chicago/Turabian StyleZhiqiang Shen; Nannan Hou; Min Jin; Zhigang Qiu; Jingfeng Wang; Bin Zhang; Xinwei Wang; Jie Wang; Dongsheng Zhou; Junwen Li. 2014. "A novel enzyme-linked immunosorbent assay for detection of Escherichia coli O157:H7 using immunomagnetic and beacon gold nanoparticles." Gut Pathogens 6, no. 1: 14-14.
Despite the health risks posed by waterborne human rotavirus (HRV), little information is available concerning the effectiveness of chlorine or chlorine dioxide (ClO2), two common disinfectants of public water sources, against HRV and their effects on its genome remain poorly understood. This study investigated the effects of chlorine and ClO2 on purified HRV by using cell culture and RT-PCR to assess virus infectivity and genetic integrity, respectively. The disinfection efficacy of ClO2 was found to be higher than that of chlorine. According to the efficiency factor Hom model, Ct value (mg/L min) ranges required for a 4-log reduction of HRV at 20 °C by chlorine and ClO2 were 5.55-5.59 and 1.21-2.47 mg/L min, respectively. Detection of the 11 HRV genome segments revealed that damage to the 1227-2354 bp of the VP4 gene was associated with the disappearance of viral infectivity by chlorine. However, no complete accordance between culturing and RT-PCR assays was observed after treatment of HRV with ClO2. These results collectively indicate that the current practice of chlorine disinfection may be inadequate to manage the risk of waterborne HRV infection, and offer the potential to monitor the infectivity of HRV adapting PCR-based protocols in chlorine disinfection.
Bin Xue; Min Jin; Dong Yang; Xuan Guo; Zhaoli Chen; Zhiqiang Shen; Xinwei Wang; Zhigang Qiu; Jingfeng Wang; Bin Zhang; Junwen Li. Effects of chlorine and chlorine dioxide on human rotavirus infectivity and genome stability. Water Research 2013, 47, 3329 -3338.
AMA StyleBin Xue, Min Jin, Dong Yang, Xuan Guo, Zhaoli Chen, Zhiqiang Shen, Xinwei Wang, Zhigang Qiu, Jingfeng Wang, Bin Zhang, Junwen Li. Effects of chlorine and chlorine dioxide on human rotavirus infectivity and genome stability. Water Research. 2013; 47 (10):3329-3338.
Chicago/Turabian StyleBin Xue; Min Jin; Dong Yang; Xuan Guo; Zhaoli Chen; Zhiqiang Shen; Xinwei Wang; Zhigang Qiu; Jingfeng Wang; Bin Zhang; Junwen Li. 2013. "Effects of chlorine and chlorine dioxide on human rotavirus infectivity and genome stability." Water Research 47, no. 10: 3329-3338.
Zhe Chen; Bin Zhang; Zhi-Qiang Sen; Zhi-Gang Qiu; Yin-Qing Guo; Jun-Wen Li; Jing-Feng Wang. [Characteristics of the extracellular polymeric substances of a heterotrophic nitrifying bacterium strain]. Huan jing ke xue= Huanjing kexue 2012, 33, 1 .
AMA StyleZhe Chen, Bin Zhang, Zhi-Qiang Sen, Zhi-Gang Qiu, Yin-Qing Guo, Jun-Wen Li, Jing-Feng Wang. [Characteristics of the extracellular polymeric substances of a heterotrophic nitrifying bacterium strain]. Huan jing ke xue= Huanjing kexue. 2012; 33 (4):1.
Chicago/Turabian StyleZhe Chen; Bin Zhang; Zhi-Qiang Sen; Zhi-Gang Qiu; Yin-Qing Guo; Jun-Wen Li; Jing-Feng Wang. 2012. "[Characteristics of the extracellular polymeric substances of a heterotrophic nitrifying bacterium strain]." Huan jing ke xue= Huanjing kexue 33, no. 4: 1.
Recently, the potential risks of nanomaterials and the spread of antibiotic resistance have become two important environmental concerns. The conjugative transfer of antibiotic resistance genes between bacteria is the most important pathway for the acquisition of antibiotic resistance by bacteria. Both environmental and genetic factors influence the conjugative transfer of antibiotic resistance genes in bacterial populations. The extent to which nanomaterials are able to bring about an increase in antibiotic resistance by regulating the conjugative transfer of antibiotic resistance genes in bacteria is still unknown. In this paper, an Orthogonal Design L64 (421) was used in duplicate to evaluate the effects of bacterial concentration, nano-alumina concentration, mating time, mating temperature and the interactions of those factors on the conjugation transfer in LB broth. The mechanisms by which nano-alumina promote the horizontal transfer of antibiotic multiresistance features were explored by morphological, biochemical, and molecular biological methods. We have shown that nano-alumina promotes the horizontal conjugative transfer multiresistance genes mediated by RP4 up to 250-fold in LB broth and 100-fold in PBS. And it would appear that the effect of promoting conjugative transfer of nano-alumina exceeds the effects of mating temperature and mating time. We also explored the mechanisms behind this phenomenon and demonstrated that nano-alumina is able to induce oxidative stress, cause the damage of bacterial cell membranes, enhance the transcriptional activity of conjugative genes and depress the global regulatory factor genes expression. The findings in this study support the notion that nano-alumina in the environment could result in ecological hazards. More important, an enhanced rate of plasmid transfer among micro-organisms may have an enormous impact on human health and environmental safety.
Zhigang Qiu; Ng Yang; Min Jin; Liangping Hu; Zhaoli Chen; Zhiqiang Shen; Jingfeng Wang; Bin Zhang; Sha Liu; Yunmei Yu; Jun-Wen Li. The Conjugative Transfer of the Multiresistance Gene Between Bacteria is Significantly Promoted by Nano-Alumina. Journal of Nanomedicine & Nanotechnology 2012, 3, 1 -9.
AMA StyleZhigang Qiu, Ng Yang, Min Jin, Liangping Hu, Zhaoli Chen, Zhiqiang Shen, Jingfeng Wang, Bin Zhang, Sha Liu, Yunmei Yu, Jun-Wen Li. The Conjugative Transfer of the Multiresistance Gene Between Bacteria is Significantly Promoted by Nano-Alumina. Journal of Nanomedicine & Nanotechnology. 2012; 3 (8):1-9.
Chicago/Turabian StyleZhigang Qiu; Ng Yang; Min Jin; Liangping Hu; Zhaoli Chen; Zhiqiang Shen; Jingfeng Wang; Bin Zhang; Sha Liu; Yunmei Yu; Jun-Wen Li. 2012. "The Conjugative Transfer of the Multiresistance Gene Between Bacteria is Significantly Promoted by Nano-Alumina." Journal of Nanomedicine & Nanotechnology 3, no. 8: 1-9.
Jing-Feng Wang; Min Jin; Zhi-Qiang Shen; Zhi-Gang Qiu; Bin Zhang; Qing-Xin Kong; Jun-Wen Li. [Screening of the aerobic simultaneous denitrobacteria and its denitrification characteristics]. Huan jing ke xue= Huanjing kexue 2011, 32, 1 .
AMA StyleJing-Feng Wang, Min Jin, Zhi-Qiang Shen, Zhi-Gang Qiu, Bin Zhang, Qing-Xin Kong, Jun-Wen Li. [Screening of the aerobic simultaneous denitrobacteria and its denitrification characteristics]. Huan jing ke xue= Huanjing kexue. 2011; 32 (8):1.
Chicago/Turabian StyleJing-Feng Wang; Min Jin; Zhi-Qiang Shen; Zhi-Gang Qiu; Bin Zhang; Qing-Xin Kong; Jun-Wen Li. 2011. "[Screening of the aerobic simultaneous denitrobacteria and its denitrification characteristics]." Huan jing ke xue= Huanjing kexue 32, no. 8: 1.