A willow short rotation coppice (SRC) trial was conducted on former mining land in northern Spain over a period of five years, with the purpose of evaluating the effects on yield of two planting densities (9876 and 14,815 cuttings ha⁻¹), three treatments (control, two levels of nitrogen, phosphorus and potassium compound fertilizer (NPK) plus weed control) and three willow clones (Björn, Inger, Olof). The area was subsoiled, ploughed, harrowed and fertilized with NPK before trial establishment. A randomized block design was applied, with three replications of each treatment in a total of 54 plots, each of an area of 400 m². The effects of the interactions between the various factors on yield and other growth parameters were also studied. The clone factor significantly affected the number of shoots per stool (greatest for the Inger clone) and the Olof clone, which showed the lowest mortality rate and produced the largest trees and largest quantity of biomass. The combined application of fertilizer and herbicide also significantly increased the values of all response variables considered, except the mortality rate. The planting density did not significantly affect the response variables. Clone × treatment interactions were significant for the shoots per stool, height, diameter and biomass variables, and the Olof clone displayed the highest height and diameter growth and yield. The results obtained in the first rotation indicate that the Olof clone adapted well to the trial conditions and therefore would be appropriate for producing biomass in abandoned mine land in Asturias. These findings will help in the development of strategies for the establishment and management of SRC on marginal land.