Traditional villages are an important cultural heritage left by China’s agrarian civilization and serve as a testament to the historical development of the Chinese nation. The study of spatial and cultural differentiation in traditional villages is significant for their future preservation and development. Existing studies predominantly adopt a macro perspective, focusing on large-scale regions, and lack investigations from a micro perspective in medium- and small-scale areas. This study utilizes ArcGIS 10.8 for spatial analysis, multi-factor geographic detectors, and cultural geography spatial zoning methods to explore the spatial structure and cultural differentiation of 305 traditional villages in western Henan. The results indicate that the distribution of traditional villages in this region is significantly clustered and uneven, primarily concentrated in specific districts of Sanmenxia and Luoyang. Per capita GDP and the distance to roads are negatively correlated with the distribution of traditional settlements, reflecting the positive impact of lower economic levels and remote locations on village preservation. The spatial layout of traditional villages in western Henan exhibits clustering patterns, with cultural zoning characterized by distinct residential forms. This study, through the analysis of the spatial structure characteristics and influencing factors of traditional villages in the western Henan region, provides a new perspective on the formation and evolution of traditional villages, revealing the cultural differentiation characteristics of western Henan. The research results offer directional guidance for the conservation strategies of traditional villages in western Henan and provide a decision-making reference for cultural heritage conservation practices in similar regions.