The planting of inter-row cover vegetation has long been practised in Western European fruit-growing regions, yet research in this area remains limited in many countries. With the rise of organic farming, the role and selection of inter-row cover species are becoming increasingly important. Proper species selection enhances weed control and promotes biodiversity, supporting flowering plants and beneficial insects in orchards. The six-year research aimed to develop a multi-species, perennial, multifunctional inter-row cover species mixture for a dry continental climate, which is suitable for soil protection and the displacement of heat-loving, drought-tolerant, invasive weed species. There was another expectation for this native seed mixture to be able to regenerate spontaneously and to be sustainable in the orchard for a long time with minimal maintenance. Based on our results, the two multi-species mixtures (15 and 18 species) successfully reduced the cover of weed species, and their composition changed in the direction of the natural flora characteristic of the area. According to general management practices, native species could not widely appear in the control rows created by mowing the local weed flora.