Pomegranate (Punica granatum) is an ancient perennial plant species of the Punicaceae family and is regarded as the ‘miracle fruit’ for its seeds being consumed as food, juice and as a functional food. Significant modern pharmacological and clinical evidence has highlighted the wide medicinal applications of pomegranate fruit parts and its juice. Pomegranate juice (PJ) that is superior to other fruit juices is a fortified source of dietary polyphenols with potential antioxidant capacity. Polyphenols of PJ include tannins, anthocyanins, and flavonoids. The presence of these beneficial phytochemicals is directly linked to its favourable health benefits viz., obesity and diabetes management and anti-inflammatory effects. This comprehensive review capitalizes on PJ with emphasis on the interrelationship between its holistic chemical composition, metabolism and biological effects. Moreover, the review recapitulates on the diverse health benefits of PJ and related patents in the field of PJ production to ensure the best produced juice quality.

Gold nanoparticles (Au) have attracted considerable attention in the field of biomedicine in recent years. The present work was designed to investigate gold nanoparticles obtained using a soil-associated actinomycetes, Streptomyces sp. ASM19. This microorganism was isolated and identified using DNA sequencing. The chemical profile of the Streptomyces sp. ASM19 extract was analyzed using LC-HRES-MS. Streptomyces sp. ASM19 extract was utilized to synthesize actinomycetes-based gold nanoparticles (Ac-AuNPs), which were analyzed using ultraviolet-visible (UV-vis) spectrophotometry, Fourier transform infrared (FTIR) spectroscopy, and atomic force microscopy (AFM). In addition, the antibacterial, and anti-biofilm, as well as, the anti-proliferative properties of Ac-AuNPs against seven cancer lines were investigated. LC-HRES-MS analysis traced a total of 111 peaks, 14 of them are key peaks belonging to the chemical classes, alkaloids, steroids, and polyketides. Analysis of the synthesized Ac-AuNPs revealed that they exhibited a wine-red color and a plasmon band appeared at 540 nm, confirming the formation of the Ac-AuNPs. Further, FTIR confirmed various functional groups present in Ac-AuNPs. The crude extract of Streptomyces sp. ASM19 demonstrated consistent antibacterial activity in contrast to Ac-AuNPs. The anti-proliferative properties of Ac-AuNPs demonstrated encouraging anticancer properties against SCC9 and SCC25 cell lines with IC₅₀ values of 3.77 and 1.56 μg mL⁻¹. Furthermore, Ac-AuNPs had total apoptotic percentages of 26.37% (SCC9) and 32.08% (SCC25), which are around 25 times higher than the control (0.95%). Additionally, it caused a notable G2/M phase cell-cycle arrest. On the other hand, molecular docking study carried out for the annotated compounds; tomaymycin (8) and nocapyrone P (5), showed considerable binding affinities compared to the co-crystallized inhibitor (fisetin) against the cyclin-dependent kinase 6 active site. Overall, the present study could be useful for nano drug delivery and may be applied for clinical studies in the future.

Cheese is one of the most common fermented dairy foods consumed worldwide. Despite being extensively used, the heating effect on cheese flavor and chemistry has yet to be reported. The impact of heat on cheese aroma profile was assessed using headspace solid‐phase microextraction (SPME) coupled to gas chromatography coupled to mass spectrometry (GC‐MS). A total of 50 volatiles were identified to encompass fatty acid‐derived compounds (82%, 75%, and 87%), nitrogenous (7%, 12%, and 10%), aromatics (6%, 2%, and 0.8%), amino acids derived volatiles (1%, 5%, and 0.7%), sugar derived volatile organic compounds (1%–3%), and terpenes (0.5%–3%) in the heated edam, emmental, and gouda cheese, respectively. The fatty acid‐derived volatiles (methyl ketones and fatty acids) are likely to contribute to its flavor amounting to 75%–87% in heated gouda, edam, and emmental cheese aroma profile. Nitrogenous compounds amounted for the second abundant class in all heated cheese at 7%–12% represented by alkyl pyrazines, whereas N‐nitrosopiperidine a toxic chemical was found only in heated emmental cheese. This study provides the first approach for the use of cold extraction techniques such as SPME coupled with GC‐MS to characterize the aroma profile of three major heated cheese, that is, edam, emmental, and gouda analyzed using chemometric tools in comparison to cold cheese. This study is the first to discuss the generation or not of hazardous compounds (N‐nitrosopiperidine) in heated cheese that could negatively affect human health upon consumption.

The anti-cancer and anti-bacterial potential of the Red Sea sponge Phyllospongia lamellosa in its bulk (crude extracts) and gold nanostructure (loaded on gold nanaoparticles) were investigated. Metabolomics analysis was conducted, and subsequently, molecular modeling studies were conducted to explore and anticipate the P. lamellosa secondary metabolites and their potential target for their various bioactivities. The chloroformic extract (CE) and ethyl acetate extract (EE) of the P. lamellosa predicted to include bioactive lipophilic and moderately polar metabolites, respectively, were used to synthesize gold nanoparticles (AuNPs). The prepared AuNPs were characterized through transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FTIR), and UV–vis spectrophotometric analyses. The cytotoxic activities were tested against MCF-7, MDB-231, and MCF-10A. Moreover, the anti-bacterial, antifungal, and anti-biofilm activity were assessed. Definite classes of metabolites were identified in CE (terpenoids) and EE (brominated phenyl ethers and sulfated fatty amides). Molecular modeling involving docking and molecular dynamics identified Protein-tyrosine phosphatase 1B (PTP1B) as a potential target for the anti-cancer activities of terpenoids. Moreover, CE exhibited the most powerful activity against breast cancer cell lines, matching our molecular modeling study. On the other hand, only EE was demonstrated to possess powerful anti-bacterial and anti-biofilm activity against Escherichia coli. In conclusion, depending on their bioactive metabolites, P. lamellosa-derived extracts, after being loaded on AuNPs, could be considered anti-cancer, anti-bacterial, and anti-biofilm bioactive products. Future work should be completed to produce drug leads.