Chemical modifications of oligonucleotides are routinely employed to enhance their functional properties. Amino‐modifiers serve as versatile chemical handles for postsynthetic (bio)conjugation, nucleic acid immobilization on solid supports, and investigations into nonenzymatic genome replication relevant to the origins of life, to name a few. Here, we report a cost‐effective, disulfide‐containing solid‐support linkage that enables the on‐column synthesis of nucleic acids with 3′‐amino or 3′‐phosphate modifications. The orthogonality of this solid‐support linker facilitates an on‐column protecting group strategy, enabling the synthesis of DNA and RNA containing 3′‐amino‐2′,3′‐dideoxyribosides from commercial unprotected mononucleosides. Additionally, we present an on‐column deprotection protocol for DNA and RNA, prior to cleavage from the solid support, eliminating the precipitation step typically required in conventional RNA workflows, leading to higher recovery for certain strands. Expanding on our previous work, we introduce a versatile modular tandem oligonucleotide synthesis (mTOS) approach, allowing selective release of downstream strands from the one directly bound to the solid‐support via the disulfide‐containing linker. Together, these advances in solid‐support design and oligonucleotide synthesis unlock new opportunities in bioconjugation, biotechnology, and the study of prebiotic replication mechanisms, broadening the utility of chemically modified nucleic acids across research disciplines.

Magneto-priming (MP) has been reported as a sustainable method to enhance crop yield and resistance to adverse environmental conditions; however, any physiochemical basis for these findings remains unavailable. In the present study, soybean (Glycine max) seeds (n = 232) were exposed to a static magnetic field (SMF) of 150–205 mT for 60 min. The effect of SMF exposure on seedling mass, hypocotyl length, radicleemergence rate, total seedling height, leaf area, chlorophyl content, and physiological attributes was evaluated. Differential analysis of the cytokinin (CK) profile of seedling tissues was achieved using ultra-high-performance liquid chromatography coupled with electrospray-ionization high-resolution tandem mass spectrometry (UHPLC-(ESI)-HRMS/MS). Results indicate that MP seedlings achieved radicle emergence earlier; however, no other statistically significant differences could be established. The absence in significant improvement in the agronomic and physiological attributes measured is in stark contrast to the results reported in other studies. Nonetheless, significant differences were found in the concentrations of trans-Zeatin (tZ) and cis-Zeatin (cZ) between control and MP plants. These results are incongruent as it would be assumed that insignificant differences in agronomic and physiological properties would align with phytohormone profiles. Future work should be performed to determine if there are any meaningful bioeffects that can be assigned to MP-generated hormone changes.