Alternaria alternata and Botrytis cinerea are among the primary fungal pathogens of fruits, causing black spot and gray mold disease, respectively. They cause serious losses in yield as well as affect fruit quality. Controlling fruit postharvest diseases largely relies on the use of chemical fungicides. However, the overuse of fungicides makes the produce unsafe due to their residual effects on the environment and human health. Therefore, significant advancements are necessary to investigate and find sustainable ways to prevent postharvest disease of fruits and minimize postharvest losses. This review summarizes the recent developments in the application of biological control and other sustainable approaches in managing fruit postharvest diseases, with an emphasis on A. alternata and B. cinerea, respectively. Furthermore, several action mechanisms, challenges, and prospects for the application of biological control agents (BCAs) are also discussed. Biological control application has been proven to successfully reduce postharvest disease of fruits caused by A. alternata and B. cinerea. In recent years, it has gradually changed from being primarily an independent field to a more crucial part of integrated pest management. Due to their characteristics that are safe, eco-friendly, and non-toxic, several BCAs have also been developed and commercialized. Therefore, biological control has the potential to be a promising approach to replace the use of chemical fungicides in controlling postharvest disease of fruits.

Raisins are an important source of polyphenolic compounds in plant foods, and polyphenols are associated with antioxidant and anti-aging activity. In this work, 628 polyphenols in raisin extracts were characterized using UPLC-MS/MS, mainly including tricetin 3′-glucuronide, diisobutyl phthalate, butyl isobutyl phthalate, isoquercitrin and 6-hydroxykaempferol-7-O-glucoside. The oxidative stress in H₂O₂-induced HepG2 cells and D-gal-induced aging mice was alleviated by raisin polyphenols (RPs) via increases in the cellular levels of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH), along with decreases in malonaldehyde (MDA), reactive oxygen species (ROS) and advanced glycosylation end-products (AGEs) levels. In addition, it was observed that RPs enhanced Sirt1 and Sirt3 expression, initiating the Keap1-Nrf2 signaling pathway, by upregulating the levels of nuclear Nrf2, facilitating the expressions of the antioxidant proteins NQO1 and HO-1, and downregulating Keap1 and cytoplasmic Nrf2 protein levels in H₂O₂-induced HepG2 cells and D-gal-induced aging mice. In summary, RP exerted antioxidant and anti-aging effects via regulating the Sirt1–Nrf2 signaling pathway in vitro and in vivo.