In this study we show that intact DNA can be recovered from both alcohol- and formalin-preserved specimens of the parasitic tapeworm Bertiella studeri for >1 yr after the original fixation and can be successfully amplified and quantified using real-time polymerase chain reaction (RT-PCR). Three different DNA extraction techniques on formalin- and alcohol-fixed material were evaluated to determine which is best suited for obtaining DNA of sufficient yield and purity to be used in downstream applications such as RT-PCR. These techniques included a first organic extraction procedure with an extensive washing step based on a glycine-containing buffer, a second organic extraction procedure that omits the glycine-containing buffer and halves the number of washes in organic solvents, and a third procedure that involves the use of a silica-based DNA binding column from Qiagen^©. The quality of extracted DNA was first examined by agarose gel electrophoresis and ethidium bromide staining and the concentrations were evaluated by OD₂₆₀. We then used an RT-PCR Applied Biosystems TaqMan® Fluorogenic 5′ Nuclease Gene Expression Assay based on the detection of a universal eukaryotic 18S rRNA gene sequence to evaluate the efficiency of the 3 methods. Here we report that, first, the use of a silica column-based DNA extraction technique results in the maximum yield of DNA, on average 55% higher than for the organic extraction methods; second, the use of glycine as a formaldehyde-binding agent in the washing buffer does not necessarily result in a better DNA yield and; third, specimens preserved in ethanol result in significantly higher yields of amplifiable DNA than do specimens preserved in formalin. We, therefore, strongly recommend the use of ethanol as the appropriate fixative if specimens are to be used for molecular work. This is the first report of DNA extraction from preserved specimens of Bertiella studeri.

Coral diseases represent a prominent menace to coral reefs and to the associated ecological services they provide to the surrounding coastal communities. Studies on diseases and compromised health conditions (CHCs) on hard corals in the Southwest Indian Ocean (SWIO) are scarce, and their consequences are often overlooked. This study aimed to establish the baseline prevalence of diseases and CHC of hard corals around Rodrigues Island. Coral disease and CHC prevalence were visually assessed using 2 m × 50 m belt transects at eight sites around the island. This is the first report of four coral diseases, namely White Plague (WP), White Syndrome (WS), Black Band (BB), and Growth Anomalies (GA), and two CHCs, two forms of Pink Pigmentation Responses (PPR)—Pink Patches (PP) and Pink Line Syndrome (PLS)—observed on six genera of hard corals from the island of Rodrigues. PP on Fungia (15.92 ± 5.65%), followed by the WS on Montipora (4.67 ± 3.72%) and GA on Gardineroseris (4.16 ± 4.16%), so far unreported from the SWIO, were the most prevalent around the island. The least prevalent disease was BB on Montipora (0.13 ± 0.13%). Although the overall disease and CHC prevalence for Rodrigues Island (0.98 ± 0.30%) were much lower than the surrounding islands in the SWIO, the observations of these diseases and CHCs on hard corals and relevant environmental parameters warrant further in-depth characterization to better inform coral reefs management and conservation actions.